GB1021721A - Improvements in or relating to the production of metabolites by micro-organisms - Google Patents

Improvements in or relating to the production of metabolites by micro-organisms

Info

Publication number
GB1021721A
GB1021721A GB3782662A GB3782662A GB1021721A GB 1021721 A GB1021721 A GB 1021721A GB 3782662 A GB3782662 A GB 3782662A GB 3782662 A GB3782662 A GB 3782662A GB 1021721 A GB1021721 A GB 1021721A
Authority
GB
United Kingdom
Prior art keywords
culture
antimetabolite
parent
organism
micro
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3782662A
Inventor
George Harry Scherr
Max E Rafelson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
CONS LAB Inc
Original Assignee
CONS LAB Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by CONS LAB Inc filed Critical CONS LAB Inc
Priority to GB3782662A priority Critical patent/GB1021721A/en
Publication of GB1021721A publication Critical patent/GB1021721A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

There is obtained an increased yield of a metabolic product from a culture of a parent micro-organism by subjecting the culture to sufficient concentration of the specific antimetabolite of the metabolite which forms the metabolic product, or precursor thereof, to inhibit growth of the parent micro-organism and forming a culture of those mutant strains which survive by reason of their characteristic enhanced production of the metabolite. The culture of the parent mico-organism may be subjected to a concentration gradient of the antimetabolite, mutant strains being obtained in the form of isolated colonies of micro-organisms surviving in that part of the gradient where growth of the parent organism has ceased. Examples are directed to the isolation of (i) a mutant strain of Escherichia coli having a higher yield of nicotinic acid, nicotinamide and/or nicotinuric acid than the parent culture, employing pyridine-3-sulphonic acid as the antimetabolite, (ii) a mutant strain of yeast derived from Saccharomyces microsporus having a higher pyridoxine yield, employing isoniazid as the antimetabolite, and (iii) a mutant strain of Escherichia coli having a higher thiamine yield, employing pyrithiamine hydrogen bromide as the antimetabolite. Where the metabolites of interest are final products not in themselves essential to the organisms from whose culture they are isolated, as in the penicillin production by Penicillium chrysogenum or the fermentation by Rhizopus nigricans to give fumaric acid, the culture is subjected to an optimum concentration of an antimetabolite specific for a precursor to the final product.ALSO:There is obtained an increased yield of a metabolic product from a culture of a parent micro-organism by subjecting the culture to sufficient concentration of the specific antimetabolite of the metabolite which forms the metabolic product, or precursor thereof, to inhibit growth of the parent micro-organism and forming a culture of those mutant strains which survive by reason of their characteristic enhanced production of the metabolite. The culture of the parent micro-organism may be subjected to a concentration gradient of the antimetabolite, mutant strains being obtained in the form of isolated colonies of micro-organisms surviving in that part of the gradient where growth of the parent organism has ceased. Examples are directed to the isolation of (i) a mutant strain of Escherichia coli having a higher yield of nicotinic acid, nicotinamide and/or nicotinuric acid than the parent culture, employing pyridine-3-sulphonic acid as the antimetabolite, (ii) a mutant strain of yeast derived from Saccharomyces microsporus having a higher pyridoxine yield, employing isoniazid as the antimetabolite, and (3) a mutant strain of Escherichia coli having a higher thiamine yield, employing pyrithiamine hydrogen bromide as the antimetabolite. Where the metabolites of interest are final products not in themselves essential to the organisms from whose culture they are isolated, as in the penicillin production by Penicillium chrysogenum or the fermentation by Rhizopus nigricans to give fumaric acid, the culture is subjected to an optimum concentration of antimetabolite specific for a precursor to the final product.
GB3782662A 1962-10-05 1962-10-05 Improvements in or relating to the production of metabolites by micro-organisms Expired GB1021721A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB3782662A GB1021721A (en) 1962-10-05 1962-10-05 Improvements in or relating to the production of metabolites by micro-organisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB3782662A GB1021721A (en) 1962-10-05 1962-10-05 Improvements in or relating to the production of metabolites by micro-organisms

Publications (1)

Publication Number Publication Date
GB1021721A true GB1021721A (en) 1966-03-09

Family

ID=10399274

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3782662A Expired GB1021721A (en) 1962-10-05 1962-10-05 Improvements in or relating to the production of metabolites by micro-organisms

Country Status (1)

Country Link
GB (1) GB1021721A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4757010A (en) * 1982-03-08 1988-07-12 Institut Francais Du Petrole Production of clostridium acetobutylicum mutants of high butanol and acetone productivity, the resultant mutants and the use of these mutants in the joint production of butanol and acetone

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4757010A (en) * 1982-03-08 1988-07-12 Institut Francais Du Petrole Production of clostridium acetobutylicum mutants of high butanol and acetone productivity, the resultant mutants and the use of these mutants in the joint production of butanol and acetone

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