GB0110501D0 - Amplification process - Google Patents

Amplification process

Info

Publication number
GB0110501D0
GB0110501D0 GBGB0110501.4A GB0110501A GB0110501D0 GB 0110501 D0 GB0110501 D0 GB 0110501D0 GB 0110501 A GB0110501 A GB 0110501A GB 0110501 D0 GB0110501 D0 GB 0110501D0
Authority
GB
United Kingdom
Prior art keywords
amplification process
amplification
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GBGB0110501.4A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Government
UK Secretary of State for Defence
Original Assignee
UK Government
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UK Government, UK Secretary of State for Defence filed Critical UK Government
Priority to GBGB0110501.4A priority Critical patent/GB0110501D0/en
Publication of GB0110501D0 publication Critical patent/GB0110501D0/en
Priority to TW091106126A priority patent/TWI323284B/zh
Priority to CNB028132726A priority patent/CN1318604C/zh
Priority to KR1020037014191A priority patent/KR101039563B1/ko
Priority to NZ528919A priority patent/NZ528919A/en
Priority to CA2445864A priority patent/CA2445864C/en
Priority to AU2002249444A priority patent/AU2002249444B2/en
Priority to JP2002585667A priority patent/JP4177118B2/ja
Priority to DE60239456T priority patent/DE60239456D1/de
Priority to PCT/GB2002/001861 priority patent/WO2002088387A2/en
Priority to KR1020097025792A priority patent/KR20100005245A/ko
Priority to AT02718374T priority patent/ATE502118T1/de
Priority to GB0209052A priority patent/GB2377991B/en
Priority to EP02718374A priority patent/EP1390532B1/en
Priority to ARP020101570A priority patent/AR035238A1/es
Priority to US10/135,807 priority patent/US6951744B2/en
Priority to US11/205,667 priority patent/US7449312B2/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/525—Phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/101—Hot start

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
GBGB0110501.4A 2001-04-30 2001-04-30 Amplification process Ceased GB0110501D0 (en)

Priority Applications (17)

Application Number Priority Date Filing Date Title
GBGB0110501.4A GB0110501D0 (en) 2001-04-30 2001-04-30 Amplification process
TW091106126A TWI323284B (en) 2001-04-30 2002-03-28 Amplification process
EP02718374A EP1390532B1 (en) 2001-04-30 2002-04-22 Amplification process
DE60239456T DE60239456D1 (de) 2001-04-30 2002-04-22 Amplifizierungsverfahren
PCT/GB2002/001861 WO2002088387A2 (en) 2001-04-30 2002-04-22 Amplification process
NZ528919A NZ528919A (en) 2001-04-30 2002-04-22 Amplification process using pyrophosphate
CA2445864A CA2445864C (en) 2001-04-30 2002-04-22 A hot start amplification process using pyrophosphatase enzyme
AU2002249444A AU2002249444B2 (en) 2001-04-30 2002-04-22 Amplification process
JP2002585667A JP4177118B2 (ja) 2001-04-30 2002-04-22 増幅方法
CNB028132726A CN1318604C (zh) 2001-04-30 2002-04-22 扩增方法
KR1020037014191A KR101039563B1 (ko) 2001-04-30 2002-04-22 증폭 방법
KR1020097025792A KR20100005245A (ko) 2001-04-30 2002-04-22 핵산 증폭 반응 중에 비특이적 생산물의 생산을 억제하는 방법
AT02718374T ATE502118T1 (de) 2001-04-30 2002-04-22 Amplifizierungsverfahren
GB0209052A GB2377991B (en) 2001-04-30 2002-04-22 DNA amplification in the presence of a pyrophosphate salt and a pyrophosphatase
ARP020101570A AR035238A1 (es) 2001-04-30 2002-04-29 Un metodo para la conduccion de una reaccion de amplificacion de acido nucleico, un equipo para dicha conduccion y el uso de una enzima pirofosfatasa termoestable para llevar a cabo reacciones de amplificacion
US10/135,807 US6951744B2 (en) 2001-04-30 2002-04-30 Amplification process
US11/205,667 US7449312B2 (en) 2001-04-30 2005-08-17 Kit for conducting a polymerase chain reaction

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0110501.4A GB0110501D0 (en) 2001-04-30 2001-04-30 Amplification process

Publications (1)

Publication Number Publication Date
GB0110501D0 true GB0110501D0 (en) 2001-06-20

Family

ID=9913697

Family Applications (2)

Application Number Title Priority Date Filing Date
GBGB0110501.4A Ceased GB0110501D0 (en) 2001-04-30 2001-04-30 Amplification process
GB0209052A Expired - Fee Related GB2377991B (en) 2001-04-30 2002-04-22 DNA amplification in the presence of a pyrophosphate salt and a pyrophosphatase

Family Applications After (1)

Application Number Title Priority Date Filing Date
GB0209052A Expired - Fee Related GB2377991B (en) 2001-04-30 2002-04-22 DNA amplification in the presence of a pyrophosphate salt and a pyrophosphatase

Country Status (14)

Country Link
US (2) US6951744B2 (enExample)
EP (1) EP1390532B1 (enExample)
JP (1) JP4177118B2 (enExample)
KR (2) KR20100005245A (enExample)
CN (1) CN1318604C (enExample)
AR (1) AR035238A1 (enExample)
AT (1) ATE502118T1 (enExample)
AU (1) AU2002249444B2 (enExample)
CA (1) CA2445864C (enExample)
DE (1) DE60239456D1 (enExample)
GB (2) GB0110501D0 (enExample)
NZ (1) NZ528919A (enExample)
TW (1) TWI323284B (enExample)
WO (1) WO2002088387A2 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0112868D0 (en) 2001-05-25 2001-07-18 Secr Defence Detection system
CN1500887A (zh) * 2002-10-01 2004-06-02 ���µ�����ҵ��ʽ���� 引物伸长反应检测方法、碱基种类判别方法及其装置
EP1634965B1 (en) 2004-09-09 2010-01-20 Roche Diagnostics GmbH Real time PCR with the addition of pyrophosphatase
US20060051796A1 (en) * 2004-09-09 2006-03-09 Inga Boell Real time PCR with the addition of pyrophosphatase
GB0502010D0 (en) * 2005-02-01 2005-03-09 Enigma Diagnostics Ltd Biochemical reagents and their uses
DE602007013223D1 (de) 2006-06-01 2011-04-28 Trilink Biotechnologies San Diego Chemisch modifizierte oligonukleotidprimer zur nukleinsäureamplifikation
KR101098764B1 (ko) * 2007-10-29 2011-12-26 (주)바이오니아 안정화된 핫스타트 ｐｃｒ용 건조 조성물
CN102105481B (zh) 2008-05-27 2016-02-24 垂林克生物技术公司 用于核酸热启动扩增的化学修饰的核苷5’-三磷酸
EP2566959A1 (en) * 2010-04-30 2013-03-13 Roche Diagniostics GmbH System and method for purification and use of inorganic pyrophosphatase from aquifex aeolicus
US8349277B2 (en) * 2010-06-17 2013-01-08 Geneasys Pty Ltd Test module with microfluidic device having LOC and dialysis device for separating pathogens from other constituents in a biological sample
KR101870311B1 (ko) * 2012-03-09 2018-06-25 (주)바이오니아 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물
US9951378B2 (en) 2012-06-14 2018-04-24 Life Technologies Corporation Compositions, methods and kits for real time polymerase chain reaction (PCR)
GB201301457D0 (en) 2013-01-28 2013-03-13 Fluorogenics Ltd Freeze-dried composition
JP6467829B2 (ja) * 2014-09-03 2019-02-13 東洋紡株式会社 改良されたｒｔ−ｐｃｒ反応
CN104862288B (zh) * 2015-05-08 2019-10-29 苏州大学 一种新型温度响应性无机焦磷酸酶偶联物的合成及其在增强聚合酶链式反应中的应用
WO2017121836A1 (en) 2016-01-15 2017-07-20 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
US11618891B2 (en) 2017-06-26 2023-04-04 Thermo Fisher Scientific Baltics Uab Thermophilic DNA polymerase mutants
JP7234598B2 (ja) * 2018-01-26 2023-03-08 東ソー株式会社 核酸増幅試薬および当該試薬を用いた核酸増幅制御方法
CN113481180A (zh) * 2021-07-05 2021-10-08 吉林大学 碱性嗜热无机焦磷酸酶及其在增强聚合酶链式反应和合成udp-半乳糖反应中的应用
WO2025117716A1 (en) * 2023-11-30 2025-06-05 Watchmaker Genomics, Inc. Taq dna polymerase hotstart antibodies and methods of use thereof

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4868103A (en) 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
CH670709A5 (enExample) * 1986-04-24 1989-06-30 Univ Moskovsk
US5498523A (en) * 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
WO1990012111A1 (en) * 1989-04-12 1990-10-18 President And Fellows Of Harvard College Improved primer extension reactions
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
FI923911A7 (fi) * 1992-09-01 1994-03-02 Timo Korpela DNA-molekyylien in vitro-synteesi
US5565339A (en) 1992-10-08 1996-10-15 Hoffmann-La Roche Inc. Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5665551A (en) * 1995-09-13 1997-09-09 Roche Molecular Systems, Inc. Purified nucleic acid encoding a thermostable pyrophosphatase
US6291164B1 (en) * 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
GB9716052D0 (en) 1996-12-06 1997-10-01 Secr Defence Reaction vessels
GB9725237D0 (en) 1997-11-29 1998-01-28 Secr Defence Amplification system
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
GB9803382D0 (en) 1998-02-19 1998-04-15 Secr Defence Detection system
US6183998B1 (en) 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
KR100292883B1 (ko) * 1999-02-09 2001-06-15 박한오 피로포스페이트와 피로포스파타제를 포함하는 핫 스타트 ｐｃｒ 반응혼합물
AU4162101A (en) 2000-02-23 2001-09-03 Hope City Pyrophosphorolysis activated polymerization (pap): application to allele-specific amplification and nucleic acid sequence determination
KR20050005626A (ko) * 2003-07-07 2005-01-14 주식회사 포스코 코크스 오븐에서의 장입탄 밀도 향상방법

Also Published As

Publication number Publication date
EP1390532A2 (en) 2004-02-25
AR035238A1 (es) 2004-05-05
KR20040015216A (ko) 2004-02-18
GB2377991B (en) 2004-01-28
ATE502118T1 (de) 2011-04-15
US7449312B2 (en) 2008-11-11
WO2002088387A3 (en) 2003-12-11
CN1318604C (zh) 2007-05-30
NZ528919A (en) 2005-07-29
US20060057617A1 (en) 2006-03-16
JP2004524853A (ja) 2004-08-19
KR101039563B1 (ko) 2011-06-09
CA2445864A1 (en) 2002-11-07
CA2445864C (en) 2011-08-02
GB2377991A (en) 2003-01-29
GB0209052D0 (en) 2002-05-29
EP1390532B1 (en) 2011-03-16
CN1522305A (zh) 2004-08-18
JP4177118B2 (ja) 2008-11-05
US20030049655A1 (en) 2003-03-13
US6951744B2 (en) 2005-10-04
AU2002249444B2 (en) 2007-07-12
WO2002088387A2 (en) 2002-11-07
DE60239456D1 (de) 2011-04-28
TWI323284B (en) 2010-04-11
KR20100005245A (ko) 2010-01-14

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Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)