FR3099769B1 - Mutagénèse bactérienne par conjugaison en milieu liquide - Google Patents

Mutagénèse bactérienne par conjugaison en milieu liquide Download PDF

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Publication number
FR3099769B1
FR3099769B1 FR1908976A FR1908976A FR3099769B1 FR 3099769 B1 FR3099769 B1 FR 3099769B1 FR 1908976 A FR1908976 A FR 1908976A FR 1908976 A FR1908976 A FR 1908976A FR 3099769 B1 FR3099769 B1 FR 3099769B1
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Prior art keywords
conjugation
liquid medium
bacterial
bacterial mutagenesis
strain
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Active
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FR1908976A
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FR3099769A1 (fr
Inventor
Caroline Ranquet
Emilie Clastres
William Merre
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Bgene Genetics
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Bgene Genetics
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

L’invention concerne un procédé de conjugaison bactérienne entre une souche bactérienne donneuse et une souche bactérienne receveuse caractérisé en ce que la conjugaison est réalisée en milieu liquide et le plasmide de conjugaison est un plasmide intégratif apte à s’intégrer dans le génome de la souche bactérienne receveuse afin de produire des transconjugants. L’invention concerne en outre une utilisation du procédé selon l’une quelconque des revendications précédentes pour réaliser une mutagénèse dirigée. Pas de figure
FR1908976A 2019-08-05 2019-08-05 Mutagénèse bactérienne par conjugaison en milieu liquide Active FR3099769B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR1908976A FR3099769B1 (fr) 2019-08-05 2019-08-05 Mutagénèse bactérienne par conjugaison en milieu liquide

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1908976A FR3099769B1 (fr) 2019-08-05 2019-08-05 Mutagénèse bactérienne par conjugaison en milieu liquide
FR1908976 2019-08-05

Publications (2)

Publication Number Publication Date
FR3099769A1 FR3099769A1 (fr) 2021-02-12
FR3099769B1 true FR3099769B1 (fr) 2021-11-05

Family

ID=69810899

Family Applications (1)

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FR1908976A Active FR3099769B1 (fr) 2019-08-05 2019-08-05 Mutagénèse bactérienne par conjugaison en milieu liquide

Country Status (1)

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FR (1) FR3099769B1 (fr)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4626504A (en) 1983-07-01 1986-12-02 Lubrizol Genetics, Inc. DNA transfer vector for gram-negative bacteria
US4680264A (en) 1983-07-01 1987-07-14 Lubrizol Genetics, Inc. Class II mobilizable gram-negative plasmid
US4686184A (en) 1983-07-01 1987-08-11 Lubrizol Genetics, Inc. Gene transfer vector
DE3841453A1 (de) 1988-12-09 1990-06-13 Degussa Verfahren zum konjugativen transfer von mobilisierbaren vektoren aus e.coli in gram-positive bakterien und dafuer geeignete vektoren
EP0900271B1 (fr) 1996-01-19 2002-07-17 Novozymes Biotech, Inc. Cellules donneuses bacteriennes s'utilisant en conjugaison
AU2002306849A1 (en) * 2001-03-21 2002-10-08 Elitra Pharmaceuticals, Inc. Identification of essential genes in microorganisms
EP1652929A1 (fr) 2004-10-27 2006-05-03 Combinature Biopharm AG Plasmides pour le transfert de gènes avec la conjugaison bacterienne
PL230884B1 (pl) 2012-09-10 2018-12-31 Inst Biochemii I Biofizyki Polskiej Akademii Nauk Plazmid pomocniczy do mobilizacji, szczep bakteryjny, system o szerokim spektrum gospodarzy do wydajnej mobilizacji plazmidów, ich zastosowania oraz zestaw molekularny
FR3050997B1 (fr) * 2016-05-04 2020-11-20 Bgene Genetics Procede de mutagenese sans cicatrice
IT201600116012A1 (it) 2016-11-17 2018-05-17 St Superiore Di Sanita Vettori coniugativi selezionabili da fruttoligosaccaridi.

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Publication number Publication date
FR3099769A1 (fr) 2021-02-12

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