FR3085960B1 - Procede de culture d’un microorganisme d’interet et installation associee - Google Patents

Procede de culture d’un microorganisme d’interet et installation associee Download PDF

Info

Publication number
FR3085960B1
FR3085960B1 FR1858285A FR1858285A FR3085960B1 FR 3085960 B1 FR3085960 B1 FR 3085960B1 FR 1858285 A FR1858285 A FR 1858285A FR 1858285 A FR1858285 A FR 1858285A FR 3085960 B1 FR3085960 B1 FR 3085960B1
Authority
FR
France
Prior art keywords
microorganism
interest
culture medium
culture
contaminating microorganisms
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1858285A
Other languages
English (en)
Other versions
FR3085960A1 (fr
Inventor
Pierre-Alain Hoffmann
Evelyne Gleyses
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyanos Biotechnologies
Original Assignee
Kyanos Biotechnologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR1858285A priority Critical patent/FR3085960B1/fr
Application filed by Kyanos Biotechnologies filed Critical Kyanos Biotechnologies
Priority to EP19769756.8A priority patent/EP3850082A1/fr
Priority to CN201980059989.4A priority patent/CN112689669A/zh
Priority to US17/276,142 priority patent/US20220049211A1/en
Priority to PCT/EP2019/074424 priority patent/WO2020053362A1/fr
Priority to BR112021004625-9A priority patent/BR112021004625A2/pt
Priority to AU2019340582A priority patent/AU2019340582B2/en
Priority to CA3111895A priority patent/CA3111895C/fr
Publication of FR3085960A1 publication Critical patent/FR3085960A1/fr
Application granted granted Critical
Publication of FR3085960B1 publication Critical patent/FR3085960B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention vise un procédé (20) de culture d'au moins un microorganisme d'intérêt (28), en hétérotrophie ou mixotrophie, dans un milieu de culture (30) aqueux, des microorganismes contaminants (32) se développant naturellement dans ledit milieu de culture (30), caractérisé en ce qu'il comprend : - une étape (21) de prélèvement d'une portion du milieu de culture (30) comprenant le microorganisme d'intérêt (28) et des microorganismes contaminants (32) ; - une étape (22) de séparation physique du microorganisme d'intérêt (28) et des microorganismes contaminants (32) au sein de ladite portion de milieu de culture (30) ; - une étape (24) de lyse des microorganismes contaminants (32) ainsi séparés de sorte à produire un lysat (35) ; - une étape (25) de réintroduction dudit lysat (35) dans le milieu de culture (30).
FR1858285A 2018-09-14 2018-09-14 Procede de culture d’un microorganisme d’interet et installation associee Active FR3085960B1 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
FR1858285A FR3085960B1 (fr) 2018-09-14 2018-09-14 Procede de culture d’un microorganisme d’interet et installation associee
CN201980059989.4A CN112689669A (zh) 2018-09-14 2019-09-12 用于培养目的微生物的方法及相关设备
US17/276,142 US20220049211A1 (en) 2018-09-14 2019-09-12 Method for cultivating a microorganism of interest and associated facility
PCT/EP2019/074424 WO2020053362A1 (fr) 2018-09-14 2019-09-12 Procédé de culture d'un microorganisme d'intérêt et installation associée
EP19769756.8A EP3850082A1 (fr) 2018-09-14 2019-09-12 Procédé de culture d'un microorganisme d'intérêt et installation associée
BR112021004625-9A BR112021004625A2 (pt) 2018-09-14 2019-09-12 método de cultura de um microrganismo de interesse e instalação associada
AU2019340582A AU2019340582B2 (en) 2018-09-14 2019-09-12 Method for cultivating a microorganism of interest and associated facility
CA3111895A CA3111895C (fr) 2018-09-14 2019-09-12 Procede de culture d'un microorganisme d'interet et installation associee

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1858285A FR3085960B1 (fr) 2018-09-14 2018-09-14 Procede de culture d’un microorganisme d’interet et installation associee

Publications (2)

Publication Number Publication Date
FR3085960A1 FR3085960A1 (fr) 2020-03-20
FR3085960B1 true FR3085960B1 (fr) 2020-11-27

Family

ID=64049414

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1858285A Active FR3085960B1 (fr) 2018-09-14 2018-09-14 Procede de culture d’un microorganisme d’interet et installation associee

Country Status (8)

Country Link
US (1) US20220049211A1 (fr)
EP (1) EP3850082A1 (fr)
CN (1) CN112689669A (fr)
AU (1) AU2019340582B2 (fr)
BR (1) BR112021004625A2 (fr)
CA (1) CA3111895C (fr)
FR (1) FR3085960B1 (fr)
WO (1) WO2020053362A1 (fr)

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2756032A1 (de) * 1977-12-15 1979-06-21 Bayer Ag Neue praeparationen von mikroorganismen
US5739019A (en) * 1994-08-08 1998-04-14 Walker; Harrell L. Method of isolating and propagating microorganisms and viruses
FR2789399B1 (fr) * 1999-02-04 2001-04-13 Alpha Biotech Procede de fabrication d'extraits de micro-organismes photosynthetiques tels que notamment de spiruline
US6656721B1 (en) * 2000-08-08 2003-12-02 Roche Vitamins, Inc. Polynucleotide portions of the biotin operon from B. subtilis for use in enhanced fermentation
JP4430914B2 (ja) * 2003-10-02 2010-03-10 昭彦 ▲吉▼田 水生生態系再生工法
US8802422B2 (en) * 2007-06-01 2014-08-12 Solazyme, Inc. Renewable diesel and jet fuel from microbial sources
CN101280278A (zh) * 2008-05-20 2008-10-08 云南大学 溶藻菌的分离方法与应用
WO2010014797A2 (fr) * 2008-07-30 2010-02-04 Washington State University Research Foundation Système intégré de production de charge de biogazole
US8580540B2 (en) * 2009-05-26 2013-11-12 Solazyme, Inc. Fractionation of oil-bearing microbial biomass
US20110081706A1 (en) * 2009-10-02 2011-04-07 TransAlgae Ltd Method and system for efficient harvesting of microalgae and cyanobacteria
JP2013511283A (ja) * 2009-11-23 2013-04-04 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー 電解質の添加を伴う抽出発酵を用いたブタノールの生成方法
AU2011274791A1 (en) * 2010-07-07 2013-02-07 Phycal Llc Modification of microalgae for magnetic properties
US9044694B2 (en) * 2010-09-01 2015-06-02 Life Technologies Corporation Device for capture and lysis of microorganisms from liquids
CN101991611B (zh) * 2010-11-18 2012-09-19 秦生巨 活性生物抗菌物及其生产方法
EP2546352A1 (fr) * 2011-07-15 2013-01-16 Neste Oil Oyj Procédé de production de lipides à partir de résidus de production d'huile de palme
JP2013179868A (ja) * 2012-02-29 2013-09-12 Asahi Glass Co Ltd 微生物の培養方法
NZ707059A (en) * 2012-11-09 2019-05-31 Heliae Dev Llc Methods of culturing microorganisms in non-axenic mixotrophic conditions
CN103232116B (zh) * 2013-04-08 2014-05-28 南京师范大学 用蓝藻水华制备生物净水剂处理重金属废水的方法
ITUB20155703A1 (it) * 2015-11-18 2017-05-18 Bioreweal S R L Processo per la produzione di metano mediante conversione biologica di anidride carbonica.
CN106701580A (zh) * 2017-01-08 2017-05-24 北京工业大学 污水处理厂活性污泥中自养菌与异养菌的分离方法
CN107118966A (zh) * 2017-05-05 2017-09-01 李宜芳 原位环境微生物分离方法、土壤本源石油降解微生物分离与筛选方法

Also Published As

Publication number Publication date
AU2019340582B2 (en) 2023-04-06
FR3085960A1 (fr) 2020-03-20
CA3111895A1 (fr) 2020-03-19
US20220049211A1 (en) 2022-02-17
AU2019340582A1 (en) 2021-05-13
CN112689669A (zh) 2021-04-20
EP3850082A1 (fr) 2021-07-21
CA3111895C (fr) 2023-10-03
WO2020053362A1 (fr) 2020-03-19
BR112021004625A2 (pt) 2021-05-25

Similar Documents

Publication Publication Date Title
EP1574527A4 (fr) Procede de purification d'emulsions aqueuses de fluoropolymere, emulsions purifiees et produits finis fluores
WO2007075836A3 (fr) Procedes de detection de troubles genetiques
WO2005060696A3 (fr) Procedes et appareil permettant d'ameliorer la detection d'echantillons fondee sur des ions au moyen d'une pre-separation et d'une amplification
WO2002006529A3 (fr) Detection et traitement de polykystose renale
WO2013036140A3 (fr) Système de retenue de filière pour une mordache et son procédé d'utilisation
FR3085960B1 (fr) Procede de culture d’un microorganisme d’interet et installation associee
Paul et al. Direct, quantitative detection of Listeria monocytogenes in fresh raw whole milk by qPCR
FR2847589B1 (fr) Procede de detection universelle de microorganismes et milieu reactionnel permettant la mise en oeuvre du procede
GB0509611D0 (en) Method and device for imaging a sample
ATE403663T1 (de) Nukleinsäureamplifikation mit duplexzwischenprodukten
MA31431B1 (fr) Installation de traitement d'eau par flottation, et procede de traitement d'eau correspondant
Iwai et al. One-pot three-step continuous enzymatic synthesis of 5-fluoro-5-deoxy-d-ribose
Giacomazzi et al. Comparison of three methods of DNA extraction from cold‐smoked salmon and impact of physical treatments
FR2819266B1 (fr) Milieu de culture pour la detection et/ou la discrimination des bacteries du genre listeria et procede de mise en oeuvre
AUPR536501A0 (en) Method for removing oil, fat and grease from water
WO2016051177A3 (fr) Procédés et kits
AU2018250570A1 (en) Methods for multiplex detection of alleles associated with corneal dystrophy
Vanfraechem et al. Conferencing at the crossroads between rehabilitation and restorative justice
FR3061129B1 (fr) Procede de fabrication d'un module d'isolation thermophonique pour aeronef comprenant une etape de cintrage
WO2004029299A3 (fr) Procede d'identification et de differenciation de bacteries
WO2017200333A3 (fr) Système et procédé d'analyse sémantique du comportement dans un espace
Van Rompay et al. Deep convolutional networks in view of an automatic cyberspace monitoring system
CN220533590U (zh) 一种球阀阀体加工冷却装置
Hussain Detection and Molecular Characterization of gyrA and gyrB Genes of MDR-Salmonella typhi Isolated from Clinical Sample in Sudan
AU2002351524A1 (en) New primers for the detection and identification of bacterial indicator groups and virulence factors

Legal Events

Date Code Title Description
PLFP Fee payment

Year of fee payment: 2

PLSC Publication of the preliminary search report

Effective date: 20200320

PLFP Fee payment

Year of fee payment: 3

PLFP Fee payment

Year of fee payment: 4

PLFP Fee payment

Year of fee payment: 5

PLFP Fee payment

Year of fee payment: 6