FR3085960B1 - Procede de culture d’un microorganisme d’interet et installation associee - Google Patents

Procede de culture d’un microorganisme d’interet et installation associee Download PDF

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Publication number
FR3085960B1
FR3085960B1 FR1858285A FR1858285A FR3085960B1 FR 3085960 B1 FR3085960 B1 FR 3085960B1 FR 1858285 A FR1858285 A FR 1858285A FR 1858285 A FR1858285 A FR 1858285A FR 3085960 B1 FR3085960 B1 FR 3085960B1
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FR
France
Prior art keywords
microorganism
interest
culture medium
culture
contaminating microorganisms
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1858285A
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English (en)
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FR3085960A1 (fr
Inventor
Pierre-Alain Hoffmann
Evelyne Gleyses
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyanos Biotechnologies
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Kyanos Biotechnologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR1858285A priority Critical patent/FR3085960B1/fr
Application filed by Kyanos Biotechnologies filed Critical Kyanos Biotechnologies
Priority to US17/276,142 priority patent/US20220049211A1/en
Priority to CN201980059989.4A priority patent/CN112689669A/zh
Priority to CA3111895A priority patent/CA3111895C/fr
Priority to BR112021004625-9A priority patent/BR112021004625A2/pt
Priority to PCT/EP2019/074424 priority patent/WO2020053362A1/fr
Priority to AU2019340582A priority patent/AU2019340582B2/en
Priority to EP19769756.8A priority patent/EP3850082A1/fr
Publication of FR3085960A1 publication Critical patent/FR3085960A1/fr
Application granted granted Critical
Publication of FR3085960B1 publication Critical patent/FR3085960B1/fr
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention vise un procédé (20) de culture d'au moins un microorganisme d'intérêt (28), en hétérotrophie ou mixotrophie, dans un milieu de culture (30) aqueux, des microorganismes contaminants (32) se développant naturellement dans ledit milieu de culture (30), caractérisé en ce qu'il comprend : - une étape (21) de prélèvement d'une portion du milieu de culture (30) comprenant le microorganisme d'intérêt (28) et des microorganismes contaminants (32) ; - une étape (22) de séparation physique du microorganisme d'intérêt (28) et des microorganismes contaminants (32) au sein de ladite portion de milieu de culture (30) ; - une étape (24) de lyse des microorganismes contaminants (32) ainsi séparés de sorte à produire un lysat (35) ; - une étape (25) de réintroduction dudit lysat (35) dans le milieu de culture (30).
FR1858285A 2018-09-14 2018-09-14 Procede de culture d’un microorganisme d’interet et installation associee Active FR3085960B1 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
FR1858285A FR3085960B1 (fr) 2018-09-14 2018-09-14 Procede de culture d’un microorganisme d’interet et installation associee
CN201980059989.4A CN112689669A (zh) 2018-09-14 2019-09-12 用于培养目的微生物的方法及相关设备
CA3111895A CA3111895C (fr) 2018-09-14 2019-09-12 Procede de culture d'un microorganisme d'interet et installation associee
BR112021004625-9A BR112021004625A2 (pt) 2018-09-14 2019-09-12 método de cultura de um microrganismo de interesse e instalação associada
US17/276,142 US20220049211A1 (en) 2018-09-14 2019-09-12 Method for cultivating a microorganism of interest and associated facility
PCT/EP2019/074424 WO2020053362A1 (fr) 2018-09-14 2019-09-12 Procédé de culture d'un microorganisme d'intérêt et installation associée
AU2019340582A AU2019340582B2 (en) 2018-09-14 2019-09-12 Method for cultivating a microorganism of interest and associated facility
EP19769756.8A EP3850082A1 (fr) 2018-09-14 2019-09-12 Procédé de culture d'un microorganisme d'intérêt et installation associée

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1858285A FR3085960B1 (fr) 2018-09-14 2018-09-14 Procede de culture d’un microorganisme d’interet et installation associee

Publications (2)

Publication Number Publication Date
FR3085960A1 FR3085960A1 (fr) 2020-03-20
FR3085960B1 true FR3085960B1 (fr) 2020-11-27

Family

ID=64049414

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1858285A Active FR3085960B1 (fr) 2018-09-14 2018-09-14 Procede de culture d’un microorganisme d’interet et installation associee

Country Status (8)

Country Link
US (1) US20220049211A1 (fr)
EP (1) EP3850082A1 (fr)
CN (1) CN112689669A (fr)
AU (1) AU2019340582B2 (fr)
BR (1) BR112021004625A2 (fr)
CA (1) CA3111895C (fr)
FR (1) FR3085960B1 (fr)
WO (1) WO2020053362A1 (fr)

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2756032A1 (de) * 1977-12-15 1979-06-21 Bayer Ag Neue praeparationen von mikroorganismen
US5739019A (en) * 1994-08-08 1998-04-14 Walker; Harrell L. Method of isolating and propagating microorganisms and viruses
FR2789399B1 (fr) * 1999-02-04 2001-04-13 Alpha Biotech Procede de fabrication d'extraits de micro-organismes photosynthetiques tels que notamment de spiruline
US6656721B1 (en) * 2000-08-08 2003-12-02 Roche Vitamins, Inc. Polynucleotide portions of the biotin operon from B. subtilis for use in enhanced fermentation
JP4430914B2 (ja) * 2003-10-02 2010-03-10 昭彦 ▲吉▼田 水生生態系再生工法
CN101765661B (zh) * 2007-06-01 2014-08-06 索拉兹米公司 在微生物中生产油
CN101280278A (zh) * 2008-05-20 2008-10-08 云南大学 溶藻菌的分离方法与应用
CN102089434A (zh) * 2008-07-30 2011-06-08 华盛顿州立大学研究基金会 生物燃料原料生产的集成系统
BRPI1012026A2 (pt) * 2009-05-26 2018-07-17 Solazyme Inc fracionamento de biomassa microbiana contendo óleo
US20110081706A1 (en) * 2009-10-02 2011-04-07 TransAlgae Ltd Method and system for efficient harvesting of microalgae and cyanobacteria
US8617861B2 (en) * 2009-11-23 2013-12-31 Butamax Advanced Biofuels Llc Method for producing butanol using extractive fermentation with electrolyte addition
AU2011274791A1 (en) * 2010-07-07 2013-02-07 Phycal Llc Modification of microalgae for magnetic properties
US9044694B2 (en) * 2010-09-01 2015-06-02 Life Technologies Corporation Device for capture and lysis of microorganisms from liquids
CN101991611B (zh) * 2010-11-18 2012-09-19 秦生巨 活性生物抗菌物及其生产方法
EP2546352A1 (fr) * 2011-07-15 2013-01-16 Neste Oil Oyj Procédé de production de lipides à partir de résidus de production d'huile de palme
JP2013179868A (ja) * 2012-02-29 2013-09-12 Asahi Glass Co Ltd 微生物の培養方法
CA2888493C (fr) * 2012-11-09 2022-06-07 Heliae Development, Llc Procedes de culture de microorganismes dans des conditions mixotrophes non axeniques
CN103232116B (zh) * 2013-04-08 2014-05-28 南京师范大学 用蓝藻水华制备生物净水剂处理重金属废水的方法
ITUB20155703A1 (it) * 2015-11-18 2017-05-18 Bioreweal S R L Processo per la produzione di metano mediante conversione biologica di anidride carbonica.
CN106701580A (zh) * 2017-01-08 2017-05-24 北京工业大学 污水处理厂活性污泥中自养菌与异养菌的分离方法
CN107118966A (zh) * 2017-05-05 2017-09-01 李宜芳 原位环境微生物分离方法、土壤本源石油降解微生物分离与筛选方法

Also Published As

Publication number Publication date
CA3111895A1 (fr) 2020-03-19
CA3111895C (fr) 2023-10-03
BR112021004625A2 (pt) 2021-05-25
US20220049211A1 (en) 2022-02-17
AU2019340582B2 (en) 2023-04-06
FR3085960A1 (fr) 2020-03-20
AU2019340582A1 (en) 2021-05-13
EP3850082A1 (fr) 2021-07-21
CN112689669A (zh) 2021-04-20
WO2020053362A1 (fr) 2020-03-19

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