FR3079846B1 - Procede de purification de proteines a partir d’extrait de microalgues - Google Patents

Procede de purification de proteines a partir d’extrait de microalgues Download PDF

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Publication number
FR3079846B1
FR3079846B1 FR1853053A FR1853053A FR3079846B1 FR 3079846 B1 FR3079846 B1 FR 3079846B1 FR 1853053 A FR1853053 A FR 1853053A FR 1853053 A FR1853053 A FR 1853053A FR 3079846 B1 FR3079846 B1 FR 3079846B1
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FR
France
Prior art keywords
purifying proteins
microalgae extract
microalgae
extract
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1853053A
Other languages
English (en)
Other versions
FR3079846A1 (fr
Inventor
Sebastien Chollet
Luc Marchal
Anthony Masse
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite de Nantes
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite de Nantes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Universite de Nantes filed Critical Centre National de la Recherche Scientifique CNRS
Priority to FR1853053A priority Critical patent/FR3079846B1/fr
Priority to PCT/FR2019/050811 priority patent/WO2019197760A1/fr
Priority to EP19720982.8A priority patent/EP3775252A1/fr
Publication of FR3079846A1 publication Critical patent/FR3079846A1/fr
Application granted granted Critical
Publication of FR3079846B1 publication Critical patent/FR3079846B1/fr
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1864—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns
    • B01D15/1885—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using two or more columns placed in parallel
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • B01D15/1892—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns the sorbent material moving as a whole, e.g. continuous annular chromatography, true moving beds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/405—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Procédé de purification de protéines comprenant une étape de purification mettant en œuvre une chromatographie de partage centrifuge, dite CPC, à partir d'un extrait de microalgue dispersé dans un mélange comprenant un système aqueux de solvants à deux phases.
FR1853053A 2018-04-09 2018-04-09 Procede de purification de proteines a partir d’extrait de microalgues Active FR3079846B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR1853053A FR3079846B1 (fr) 2018-04-09 2018-04-09 Procede de purification de proteines a partir d’extrait de microalgues
PCT/FR2019/050811 WO2019197760A1 (fr) 2018-04-09 2019-04-08 Procédé de purification de protéines à partir d'extrait de microalgues
EP19720982.8A EP3775252A1 (fr) 2018-04-09 2019-04-08 Procédé de purification de protéines à partir d'extrait de microalgues

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1853053 2018-04-09
FR1853053A FR3079846B1 (fr) 2018-04-09 2018-04-09 Procede de purification de proteines a partir d’extrait de microalgues

Publications (2)

Publication Number Publication Date
FR3079846A1 FR3079846A1 (fr) 2019-10-11
FR3079846B1 true FR3079846B1 (fr) 2020-12-25

Family

ID=62873485

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1853053A Active FR3079846B1 (fr) 2018-04-09 2018-04-09 Procede de purification de proteines a partir d’extrait de microalgues

Country Status (3)

Country Link
EP (1) EP3775252A1 (fr)
FR (1) FR3079846B1 (fr)
WO (1) WO2019197760A1 (fr)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2791578B1 (fr) 1999-03-31 2001-06-08 S E A B Societes D Etudes Et D Dispositif ameliore de chromatographie de partage centrifuge a cellules
MXNL06000062A (es) 2006-09-06 2008-10-09 Itesm Recuperacion y purificacion de b-ficoeritrina producida por porphyridium cruentum utilizando sistemas de dos fases acuosas y precipitacion isoelectrica.
KR20110119071A (ko) 2010-04-26 2011-11-02 한국과학기술연구원 원심분리 분배 크로마토그래피를 사용하여 해조류에서 푸코잔틴을 분리하는 방법
FR3025202A1 (fr) 2014-08-28 2016-03-04 Algobiotech Procede d'obtention d'un precipite stable enrichi en phycobili proteines

Also Published As

Publication number Publication date
EP3775252A1 (fr) 2021-02-17
WO2019197760A1 (fr) 2019-10-17
FR3079846A1 (fr) 2019-10-11

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