FR3074018B1 - Procede de cryoconservation de cellules a visee therapeutique - Google Patents

Procede de cryoconservation de cellules a visee therapeutique Download PDF

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Publication number
FR3074018B1
FR3074018B1 FR1761213A FR1761213A FR3074018B1 FR 3074018 B1 FR3074018 B1 FR 3074018B1 FR 1761213 A FR1761213 A FR 1761213A FR 1761213 A FR1761213 A FR 1761213A FR 3074018 B1 FR3074018 B1 FR 3074018B1
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FR
France
Prior art keywords
therapeutic
composition
cell cryopreservation
cells
cryopreservation process
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1761213A
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English (en)
Other versions
FR3074018A1 (fr
Inventor
Larichaudy Joffrey De
Nemorin Sandy Cazalon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
LFB SA
Original Assignee
LFB SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by LFB SA filed Critical LFB SA
Priority to FR1761213A priority Critical patent/FR3074018B1/fr
Priority to PCT/FR2018/053012 priority patent/WO2019102172A1/fr
Priority to US16/766,900 priority patent/US11785937B2/en
Priority to EP18826418.8A priority patent/EP3716762A1/fr
Publication of FR3074018A1 publication Critical patent/FR3074018A1/fr
Application granted granted Critical
Publication of FR3074018B1 publication Critical patent/FR3074018B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/15—Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/33—Fibroblasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
    • A61K47/18—Amines; Amides; Ureas; Quaternary ammonium compounds; Amino acids; Oligopeptides having up to five amino acids
    • A61K47/183—Amino acids, e.g. glycine, EDTA or aspartame
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/20—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing sulfur, e.g. dimethyl sulfoxide [DMSO], docusate, sodium lauryl sulfate or aminosulfonic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A61K9/19—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles lyophilised, i.e. freeze-dried, solutions or dispersions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Hematology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cardiology (AREA)
  • Vascular Medicine (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention se rapporte à une composition comprenant, dans un milieu physiologiquement acceptable : a) au moins un saccharide, b) au moins un acide aminé, c) au moins un sel de bicarbonate, d) du DMSO ou au moins un alcanediol en C3-C5, et e) des cellules à visée thérapeutique, ladite composition ayant un pH compris entre 7,0 et 8,3. Elle se rapporte également à un procédé de cryoconservation d'au moins un échantillon de cellules à visée thérapeutique, comprenant les étapes suivantes : i) mélange de l'échantillon de cellules à visée thérapeutique avec les ingrédients a) à d) ci-dessus et un milieu physiologiquement acceptable, afin d'obtenir une composition ayant un pH compris entre 7,0 et 8,3, puis ii) congélation de la composition obtenue à l'étape i).
FR1761213A 2017-11-27 2017-11-27 Procede de cryoconservation de cellules a visee therapeutique Active FR3074018B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR1761213A FR3074018B1 (fr) 2017-11-27 2017-11-27 Procede de cryoconservation de cellules a visee therapeutique
PCT/FR2018/053012 WO2019102172A1 (fr) 2017-11-27 2018-11-27 Procédé de cryoconservation de cellules à visée thérapeutique
US16/766,900 US11785937B2 (en) 2017-11-27 2018-11-27 Method for the cryopreservation of cells for therapeutic purposes
EP18826418.8A EP3716762A1 (fr) 2017-11-27 2018-11-27 Procédé de cryoconservation de cellules à visée thérapeutique

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1761213A FR3074018B1 (fr) 2017-11-27 2017-11-27 Procede de cryoconservation de cellules a visee therapeutique
FR1761213 2017-11-27

Publications (2)

Publication Number Publication Date
FR3074018A1 FR3074018A1 (fr) 2019-05-31
FR3074018B1 true FR3074018B1 (fr) 2020-09-11

Family

ID=61003218

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1761213A Active FR3074018B1 (fr) 2017-11-27 2017-11-27 Procede de cryoconservation de cellules a visee therapeutique

Country Status (4)

Country Link
US (1) US11785937B2 (fr)
EP (1) EP3716762A1 (fr)
FR (1) FR3074018B1 (fr)
WO (1) WO2019102172A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2023203562A1 (fr) * 2022-04-19 2023-10-26 Enlivex Therapeutics R&D Ltd Formulation congelée comprenant des cellules apoptotiques

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE109507T1 (de) * 1989-04-28 1994-08-15 Miles Inc Grossfermenter-inokulation mit gefrorenen zellen.
US6503698B1 (en) * 2000-06-16 2003-01-07 The United States Of America As Represented By The Secretary Of Agriculture Cryopreservation of swine embryos
FR2810045B1 (fr) 2000-06-07 2004-09-03 Assist Publ Hopitaux De Paris Procede d'obtention de population cellulaires caracterisees d'origine musculaire et utilisations
US20080113431A1 (en) 2006-11-15 2008-05-15 Mariposa Biotechnology, Inc. Methods and compositions for cryopreserving oocytes
WO2015066631A2 (fr) 2013-11-01 2015-05-07 University Of Notre Dame Du Lac Milieu de culture cellulaire et optimisation des bioprocessus
FR3038325A1 (fr) 2015-06-30 2017-01-06 Lab Francais Du Fractionnement Procede de cryoconservation de cellules a visee therapeutique
FR3038324B1 (fr) * 2015-06-30 2020-10-30 Lab Francais Du Fractionnement Procede de cryoconservation de cellules a visee therapeutique

Also Published As

Publication number Publication date
US20210244016A1 (en) 2021-08-12
US11785937B2 (en) 2023-10-17
EP3716762A1 (fr) 2020-10-07
WO2019102172A1 (fr) 2019-05-31
FR3074018A1 (fr) 2019-05-31

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