FR3068361B1 - Systeme fluidique de production de vesicules extracellulaires et procede associe - Google Patents

Systeme fluidique de production de vesicules extracellulaires et procede associe Download PDF

Info

Publication number
FR3068361B1
FR3068361B1 FR1756183A FR1756183A FR3068361B1 FR 3068361 B1 FR3068361 B1 FR 3068361B1 FR 1756183 A FR1756183 A FR 1756183A FR 1756183 A FR1756183 A FR 1756183A FR 3068361 B1 FR3068361 B1 FR 3068361B1
Authority
FR
France
Prior art keywords
container
production
liquid medium
extracellular vesicles
fluidic system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1756183A
Other languages
English (en)
Other versions
FR3068361A1 (fr
Inventor
Florence Gazeau
Amanda Karine Andriola Silva
Otto-Wilhelm Merten
Claire Wilhelm
Max Piffoux
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Genethon
Universite Paris Cite
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite Paris Diderot Paris 7
Genethon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR1756183A priority Critical patent/FR3068361B1/fr
Application filed by Centre National de la Recherche Scientifique CNRS, Universite Paris Diderot Paris 7, Genethon filed Critical Centre National de la Recherche Scientifique CNRS
Priority to EP18737565.4A priority patent/EP3645700A1/fr
Priority to CN201880053335.6A priority patent/CN111108186B/zh
Priority to KR1020207002967A priority patent/KR20200034727A/ko
Priority to CA3068614A priority patent/CA3068614A1/fr
Priority to KR1020237038756A priority patent/KR20230170725A/ko
Priority to AU2018294559A priority patent/AU2018294559B2/en
Priority to US16/627,013 priority patent/US20200385665A1/en
Priority to PCT/EP2018/067704 priority patent/WO2019002608A1/fr
Priority to JP2020522409A priority patent/JP7281773B2/ja
Publication of FR3068361A1 publication Critical patent/FR3068361A1/fr
Application granted granted Critical
Publication of FR3068361B1 publication Critical patent/FR3068361B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/14—Rotation or movement of the cells support, e.g. rotated hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00—Means for pre-treatment of biological substances
    • C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products

Abstract

L'invention concerne un système fluidique de production de vésicules extracellulaires à partir de cellules productrices, comprenant au moins un récipient, un milieu liquide contenu par le récipient et des cellules productrices, caractérisé en ce qu'il comprend également des microporteurs en suspension dans le milieu liquide, la majorité des cellules productrices étant adhérentes à la surface des microporteurs, et un agitateur de milieu liquide, l'agitateur et les dimensions du récipient étant adaptés à commander un écoulement turbulent du milieu liquide dans le récipient.
FR1756183A 2017-06-30 2017-06-30 Systeme fluidique de production de vesicules extracellulaires et procede associe Active FR3068361B1 (fr)

Priority Applications (10)

Application Number Priority Date Filing Date Title
FR1756183A FR3068361B1 (fr) 2017-06-30 2017-06-30 Systeme fluidique de production de vesicules extracellulaires et procede associe
PCT/EP2018/067704 WO2019002608A1 (fr) 2017-06-30 2018-06-29 Systeme fluidique de production de vesicules extracellulaires et procede associe
KR1020207002967A KR20200034727A (ko) 2017-06-30 2018-06-29 세포외 소포를 생산하는 유체 시스템 및 관련 방법
CA3068614A CA3068614A1 (fr) 2017-06-30 2018-06-29 Systeme fluidique de production de vesicules extracellulaires et procede associe
KR1020237038756A KR20230170725A (ko) 2017-06-30 2018-06-29 세포외 소포를 생산하는 유체 시스템 및 관련 방법
AU2018294559A AU2018294559B2 (en) 2017-06-30 2018-06-29 Fluid system for producing extracellular vesicles and associated method
EP18737565.4A EP3645700A1 (fr) 2017-06-30 2018-06-29 Systeme fluidique de production de vesicules extracellulaires et procede associe
CN201880053335.6A CN111108186B (zh) 2017-06-30 2018-06-29 制备细胞外囊泡的流体系统及相关方法
JP2020522409A JP7281773B2 (ja) 2017-06-30 2018-06-29 細胞外小胞を生産する流体システムおよびそれに関連する方法
US16/627,013 US20200385665A1 (en) 2017-06-30 2018-06-29 Fluid system for producing extracellular vesicles and associated method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1756183A FR3068361B1 (fr) 2017-06-30 2017-06-30 Systeme fluidique de production de vesicules extracellulaires et procede associe
FR1756183 2017-06-30

Publications (2)

Publication Number Publication Date
FR3068361A1 FR3068361A1 (fr) 2019-01-04
FR3068361B1 true FR3068361B1 (fr) 2021-10-15

Family

ID=60202109

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1756183A Active FR3068361B1 (fr) 2017-06-30 2017-06-30 Systeme fluidique de production de vesicules extracellulaires et procede associe

Country Status (9)

Country Link
US (1) US20200385665A1 (fr)
EP (1) EP3645700A1 (fr)
JP (1) JP7281773B2 (fr)
KR (2) KR20200034727A (fr)
CN (1) CN111108186B (fr)
AU (1) AU2018294559B2 (fr)
CA (1) CA3068614A1 (fr)
FR (1) FR3068361B1 (fr)
WO (1) WO2019002608A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107964509A (zh) * 2016-10-20 2018-04-27 湖北盛齐安生物科技股份有限公司 促进细胞释放囊泡的设备及其促进细胞释放囊泡的方法
TW202122569A (zh) * 2019-08-09 2021-06-16 日商宇部興產股份有限公司 應用小片多孔膜的細胞培養法
WO2021099575A1 (fr) 2019-11-21 2021-05-27 INSERM (Institut National de la Santé et de la Recherche Médicale) Vésicules extracellulaires dérivées de cellules souches mésenchymateuses chargées d'au moins un photosensibilisant et leurs utilisations pour le traitement de la carcinomatose péritonéale
EP4149440A1 (fr) 2020-05-11 2023-03-22 Erytech Pharma Vésicules extracellulaires de globules rouges (vecgr) contenant des cargos et procédés d'utilisation et de production de celles-ci
FR3112147A1 (fr) 2020-07-02 2022-01-07 Universite De Paris Procédé de calibration d’un système fluidique pour la production de vésicules extracellulaires et système fluidique de production associé
WO2024018003A1 (fr) 2022-07-21 2024-01-25 Institut National de la Santé et de la Recherche Médicale Vésicules extracellulaires fonctionnalisées avec une syncytine d'erv et leurs utilisations pour l'administration de charge

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3529203A1 (de) * 1984-08-24 1986-02-27 Damon Biotech, Inc., Needham Heights, Mass. Gefaess zum kultivieren von zellen auf mikrotraegern oder in kapseln
JPS61257181A (ja) * 1985-05-09 1986-11-14 Teijin Ltd 動物細胞の培養装置
US4806484A (en) * 1987-08-07 1989-02-21 Igb Products, Ltd. Perfusion airlift bioreactor
GB0116038D0 (en) * 2001-06-29 2001-08-22 Middelberg Anton A protein folding reactor
GB0907260D0 (en) * 2009-04-28 2009-06-10 Ge Healthcare Uk Ltd Method and apparatus for maintaining microcarrier beads in suspension
US9745548B2 (en) * 2012-03-15 2017-08-29 Flodesign Sonics, Inc. Acoustic perfusion devices
US9567559B2 (en) * 2012-03-15 2017-02-14 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
GB201211256D0 (en) * 2012-06-25 2012-08-08 Glaxosmithkline Biolog Sa Fermentation process
US9829483B2 (en) * 2013-09-26 2017-11-28 The General Hospital Corporation Methods of isolating extracellular vesicles
WO2016168680A1 (fr) * 2015-04-17 2016-10-20 Exocyte Therapeutics Pte Ltd. Procédé de développement de vaccins à base d'exosomes
US20170121685A1 (en) 2015-11-02 2017-05-04 Tigenix S.A.U. Mesenchymal stem cell-derived exosomes and their uses

Also Published As

Publication number Publication date
KR20230170725A (ko) 2023-12-19
AU2018294559B2 (en) 2023-09-28
AU2018294559A1 (en) 2020-02-13
EP3645700A1 (fr) 2020-05-06
CN111108186A (zh) 2020-05-05
US20200385665A1 (en) 2020-12-10
JP7281773B2 (ja) 2023-05-26
CN111108186B (zh) 2023-10-24
WO2019002608A1 (fr) 2019-01-03
FR3068361A1 (fr) 2019-01-04
JP2020528763A (ja) 2020-10-01
CA3068614A1 (fr) 2019-01-03
KR20200034727A (ko) 2020-03-31

Similar Documents

Publication Publication Date Title
FR3068361B1 (fr) Systeme fluidique de production de vesicules extracellulaires et procede associe
IN2014MN02555A (fr)
EA201390238A1 (ru) Устройство и способ распределения жидкого продукта, наносимого на поверхность
MA34550B1 (fr) Procede de filtration sur membrane presentant une recuperation d'eau elevee
BR112015019492A2 (pt) câmara de gotejamento iv com filtro e reservatório de fundo
EP2639483A3 (fr) Contrôle du flux de liquide de dépôt de film
GB2521293A (en) Subsea production system with downhole equipment suspension system
CY1112902T1 (el) Συστημα καλλιεργειας και αναπαραγωγης μικροοργανισμων (για παραδειγμα αλγη) μεσω εντασης φωτος
MY165268A (en) Method for separating liquid from suspended matter in a sludge and installation therefor
MA38927B1 (fr) Système et procédés pour la propagation continue et la production de masse des champignons mycorhiziens arbusculaires
MY167049A (en) Improved flow module placement between a subsea tree and a tubing hanger spool
MA35602B1 (fr) Dispersion dans l'huile de sulfoximines pour la lutte contre les insectes
NZ729931A (en) Liquid analyser
FR3030547B1 (fr) Sirop (meth)acrylique liquide, procede d'impregnation d'un substrat fibreux par ledit sirop, et materiau composite obtenu apres polymerisation dudit sirop d'impregnation
FR3091295B1 (fr) Systeme fluidique de production de vesicules extracellulaires et procede associe
FR3047998B1 (fr) Procede de culture d'organismes photosynthetiques a l'aide d'une source de co2.
MY182774A (en) Algae culturing apparatus and algae culturing system
MA39373A1 (fr) Procédé et appareil pour la production en continu de panneaux sandwich à noyau en mousse pir/puir/pur
FR3091296B1 (fr) Systeme fluidique de production de vesicules extracellulaires comprenant un agent therapeutique ou d’imagerie et procede associe
MX2016008839A (es) Aplicacion de multi-bomba de velocidad variable para proveer ahorro de energia al calcular y compensar la perdida por friccion usando referencia de velocidad.
MX357886B (es) Equipo y procedimiento para calentar óhmicamente un líquido que contiene partículas.
FR2953686B1 (fr) Procede pour reduire la teneur bacterienne d'un milieu alimentaire et/ou biologique d'interet, contenant des gouttelettes lipidiques
FR2993096A1 (fr) Dispositif et procede de support individuel de composants
FR3076979B1 (fr) Dispositif de production de larves
FR2983491B1 (fr) Procede de multiplication de microorganismes phyto-benefiques

Legal Events

Date Code Title Description
PLSC Publication of the preliminary search report

Effective date: 20190104

PLFP Fee payment

Year of fee payment: 3

PLFP Fee payment

Year of fee payment: 4

TQ Partial transmission of property

Owner name: GENETHON, FR

Effective date: 20201127

Owner name: UNIVERSITE DE PARIS, FR

Effective date: 20201127

Owner name: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (, FR

Effective date: 20201127

PLFP Fee payment

Year of fee payment: 5

PLFP Fee payment

Year of fee payment: 6

CD Change of name or company name

Owner name: UNIVERSITE PARIS CITE, FR

Effective date: 20221006

Owner name: GENETHON, FR

Effective date: 20221006

Owner name: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (, FR

Effective date: 20221006

PLFP Fee payment

Year of fee payment: 7