FR3032719B1 - DIAGNOSTIC DEVICE BY ISOTHERMAL DNA AMPLIFICATION - Google Patents
DIAGNOSTIC DEVICE BY ISOTHERMAL DNA AMPLIFICATION Download PDFInfo
- Publication number
- FR3032719B1 FR3032719B1 FR1551189A FR1551189A FR3032719B1 FR 3032719 B1 FR3032719 B1 FR 3032719B1 FR 1551189 A FR1551189 A FR 1551189A FR 1551189 A FR1551189 A FR 1551189A FR 3032719 B1 FR3032719 B1 FR 3032719B1
- Authority
- FR
- France
- Prior art keywords
- zone
- diagnostic
- sample
- dna amplification
- diagnostic device
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
- B01L2300/126—Paper
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
Abstract
Le dispositif (10) de diagnostic par amplification isotherme d'ADN comporte : - un substrat (105) poreux comportant : - une zone (110) comportant au moins un composé (115) réactif réagissant avec un composé (120) cible d'un échantillon, dite « zone de diagnostic », - une zone (125) de dépôt de fluide transporté, par capillarité, dans la zone de dépôt d'échantillon et dans la zone de diagnostic, - la zone (130) de dépôt d'échantillon positionnée entre la zone de dépôt de fluide et la zone de diagnostic, et - deux canaux (135, 140), positionnés dans l'épaisseur du substrat poreux, respectivement entre, d'une part, la zone de dépôt de fluide et la zone de dépôt d'échantillon, d'autre part, la zone de dépôt d'échantillon et la zone de diagnostic ; et - un moyen (205) d'échauffement isotherme local la zone de diagnostic.The isothermal DNA amplification diagnostic device (10) comprises: - a porous substrate (105) comprising: - a zone (110) comprising at least one reactive compound (115) reacting with a target compound (120) of a sample, known as the “diagnostic zone”, - a zone (125) for depositing transported fluid, by capillary action, in the sample deposit zone and in the diagnostic zone, - the sample deposit zone (130) positioned between the fluid deposition zone and the diagnostic zone, and - two channels (135, 140), positioned in the thickness of the porous substrate, respectively between, on the one hand, the fluid deposition zone and the zone on the other hand, the sample deposition area and the diagnostic area; and - a means (205) of local isothermal heating of the diagnostic zone.
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1551189A FR3032719B1 (en) | 2015-02-13 | 2015-02-13 | DIAGNOSTIC DEVICE BY ISOTHERMAL DNA AMPLIFICATION |
EP16705099.6A EP3256253A1 (en) | 2015-02-13 | 2016-02-12 | Paper device for genetic diagnosis |
PCT/EP2016/053077 WO2016128570A1 (en) | 2015-02-13 | 2016-02-12 | Paper device for genetic diagnosis |
US15/550,716 US20180245144A1 (en) | 2015-02-13 | 2016-02-12 | Paper device for genetic diagnosis |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1551189 | 2015-02-13 | ||
FR1551189A FR3032719B1 (en) | 2015-02-13 | 2015-02-13 | DIAGNOSTIC DEVICE BY ISOTHERMAL DNA AMPLIFICATION |
Publications (2)
Publication Number | Publication Date |
---|---|
FR3032719A1 FR3032719A1 (en) | 2016-08-19 |
FR3032719B1 true FR3032719B1 (en) | 2020-05-15 |
Family
ID=52829148
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR1551189A Expired - Fee Related FR3032719B1 (en) | 2015-02-13 | 2015-02-13 | DIAGNOSTIC DEVICE BY ISOTHERMAL DNA AMPLIFICATION |
Country Status (1)
Country | Link |
---|---|
FR (1) | FR3032719B1 (en) |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998022625A1 (en) * | 1996-11-20 | 1998-05-28 | The Regents Of The University Of Michigan | Microfabricated isothermal nucleic acid amplification devices and methods |
GB0919159D0 (en) * | 2009-11-02 | 2009-12-16 | Sec Dep For Environment Food A | Device and apparatus |
WO2013067272A1 (en) * | 2011-11-04 | 2013-05-10 | Diagnostics For All, Inc. | Low cost, disposable molecular diagnostic devices |
-
2015
- 2015-02-13 FR FR1551189A patent/FR3032719B1/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
FR3032719A1 (en) | 2016-08-19 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PLFP | Fee payment |
Year of fee payment: 2 |
|
PLSC | Publication of the preliminary search report |
Effective date: 20160819 |
|
PLFP | Fee payment |
Year of fee payment: 3 |
|
TQ | Partial transmission of property |
Owner name: ECOLE SUPERIEURE DE PHYSIQUE ET DE CHIMIE INDU, FR Effective date: 20170324 Owner name: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, FR Effective date: 20170324 |
|
PLFP | Fee payment |
Year of fee payment: 4 |
|
PLFP | Fee payment |
Year of fee payment: 6 |
|
PLFP | Fee payment |
Year of fee payment: 7 |
|
ST | Notification of lapse |
Effective date: 20221005 |