FR2966826B1 - Procede de concentration de composes echantillons et d'amplification d'acides nucleiques - Google Patents

Procede de concentration de composes echantillons et d'amplification d'acides nucleiques

Info

Publication number
FR2966826B1
FR2966826B1 FR1159711A FR1159711A FR2966826B1 FR 2966826 B1 FR2966826 B1 FR 2966826B1 FR 1159711 A FR1159711 A FR 1159711A FR 1159711 A FR1159711 A FR 1159711A FR 2966826 B1 FR2966826 B1 FR 2966826B1
Authority
FR
France
Prior art keywords
nucleic acids
amplifying nucleic
sample compounds
concentrating sample
concentrating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1159711A
Other languages
English (en)
Other versions
FR2966826A1 (fr
Inventor
Susanne Muench
Jan Weile
Peter Rothacher
Martina Daub
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
Original Assignee
Robert Bosch GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Robert Bosch GmbH filed Critical Robert Bosch GmbH
Publication of FR2966826A1 publication Critical patent/FR2966826A1/fr
Application granted granted Critical
Publication of FR2966826B1 publication Critical patent/FR2966826B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
FR1159711A 2010-10-27 2011-10-26 Procede de concentration de composes echantillons et d'amplification d'acides nucleiques Active FR2966826B1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102010043015.3A DE102010043015B4 (de) 2010-10-27 2010-10-27 Verfahren zur Konzentration von Probenbestandteilen und Amplifikation von Nukleinsäuren

Publications (2)

Publication Number Publication Date
FR2966826A1 FR2966826A1 (fr) 2012-05-04
FR2966826B1 true FR2966826B1 (fr) 2014-08-08

Family

ID=45373347

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1159711A Active FR2966826B1 (fr) 2010-10-27 2011-10-26 Procede de concentration de composes echantillons et d'amplification d'acides nucleiques

Country Status (5)

Country Link
US (1) US9434980B2 (fr)
CN (1) CN102453710A (fr)
DE (1) DE102010043015B4 (fr)
FR (1) FR2966826B1 (fr)
GB (1) GB2485051B (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103484353A (zh) * 2012-06-12 2014-01-01 清华大学 基于滤纸的生物大分子提取装置
DE102014207775A1 (de) * 2014-04-25 2015-10-29 Robert Bosch Gmbh Verfahren und mikrofluidisches System zum Aufbereiten von organischen Zellen und Herstellungsverfahren zum Herstellen eines mikrofluidischen Systems zum Aufbereiten von organischen Zellen
DE102014207774B4 (de) * 2014-04-25 2015-12-31 Robert Bosch Gmbh Verfahren und Vorrichtung zur Aufreinigung von biologischen Molekülen

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5348855A (en) * 1986-03-05 1994-09-20 Miles Inc. Assay for nucleic acid sequences in an unpurified sample
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
DE19746874A1 (de) * 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
GB2337261B (en) * 1998-05-15 2002-09-18 Fsm Technologies Ltd Separation of nucleic acids
AU2002318631B2 (en) 2001-07-09 2007-08-02 Asahi Kasei Kabushiki Kaisha Method of purifying nucleic acid using nonwoven fabric and detection method
GR1004106B (el) 2002-01-24 2003-01-13 Εκεφε "Δημοκριτος" Ινστιτουτο Μικροηλεκτρονικης Ολοκληρωμενοι θερμικοι αισθητηρες πυριτιου χαμηλης ισχυος και διαταξεις μικρο-ροης βασισμενοι στην χρηση τεχνολογιας κοιλοτητας αερα σφραγισμενης με μεμβρανη πορωδους πυριτιου ή τεχνολογιας μικρο-καναλιων
US7597936B2 (en) * 2002-11-26 2009-10-06 University Of Utah Research Foundation Method of producing a pigmented composite microporous material
EP1592505A2 (fr) 2003-01-21 2005-11-09 Micronics, Inc. Procede et systeme de manipulation microfluidique, amplification et analyse de fluides tels les essais de bacteries et tests antiglobuliniques
EP1871527B1 (fr) * 2004-12-23 2017-09-27 Abbott Point of Care Inc. Systeme de diagnostic moleculaire
FR2885140B1 (fr) * 2005-04-29 2010-12-31 Millipore Corp Procede de detection et de caracterisation de microorgarnismes sur une membrane.
KR100912530B1 (ko) 2007-11-13 2009-08-18 한국전자통신연구원 일회용 다층형 혈장 분리 필터 소자
KR100912531B1 (ko) 2007-12-17 2009-08-18 한국전자통신연구원 필터칩 및 그 제조 방법
FR2926560A1 (fr) * 2008-01-21 2009-07-24 Millipore Corp Procede d'extraction et de purification d'acides nucleiques sur membrane
EP2337852A4 (fr) * 2008-09-30 2012-03-14 Univ Northwestern Procédés et compositions pour isoler un acide nucléique
EP2356249B1 (fr) 2008-10-10 2016-02-17 Picovitro Ab Analyse génétique dans des micropuits

Also Published As

Publication number Publication date
US20120135471A1 (en) 2012-05-31
US9434980B2 (en) 2016-09-06
FR2966826A1 (fr) 2012-05-04
GB201118363D0 (en) 2011-12-07
DE102010043015A1 (de) 2012-05-03
GB2485051B (en) 2015-12-16
CN102453710A (zh) 2012-05-16
DE102010043015B4 (de) 2019-12-19
GB2485051A (en) 2012-05-02

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