FR2847426A1 - Use of fruit and vegetable extracts with peroxidase- and catalase-like ability to destroy hydrogen peroxide as dietary supplements, for skin protection and to prepare cosmetic, dermatological and veterinary products - Google Patents

Abstract

Use of edible fruit and vegetable extracts with peroxidase- and catalase-like ability to destroy hydrogen peroxide (H2O2), producing water and molecular oxygen in hydrophilic media.

Description

It has been widely demonstrated that peroxidative systems such as water

  oxygenated and lipoperoxides, lead to the formation of molecules toxic to the body.

  Free radicals, hydrogen peroxide, are oxidants produced naturally by the body, in the mitochondria. They intervene during phagocytosis, their accumulation is dangerous.

  Such peroxidative systems mainly originate from the intervention of free radicals, such as the superoxide anion 020 which generates hydrogen peroxide H202, leading to hydroxyl radicals which are involved in the lipoperoxidation process.

  However, reactive oxygen species such as the superoxide anion, hydroxyl radicals as well as oxygenated water, cause the formation of inflammatory systems, degrading a

  variety of intra and extra-cellular molecules.

  Also, the deficiency in antioxidants and protective enzymes may in certain circumstances, for example in the case of burns, no longer be able to inactivate neutrophils. Thus for some authors, an increase in the level of hydrogen peroxide and the superoxide radical produced by macrophages, would be the major cause of

  oxidative stress and the cellular damage that results from it.

  So, for example, in case of deficiency of natural antioxidants, such as reduced glutathione, the main antioxidant in the body, it has been found, the presence of hydrogen peroxide in the vitreous humor and in the retina. (MAUGER T, LANSON LJ Toxicol. Cutaneous Ocul 2 0 1992, 44 (4) 369) That epithelial cells subjected to 2 nM hydrogen peroxide show a fall in glutathione reduced by more than 80% (FERRANDELLI J and Col Neuropatho Exp. Neurol. 1971 60 638) That hydrogen peroxide induces the oxidative stress of the heart muscle (JANERO D and Col. J. Cell.Physiol. 1991 149 (3) 247) In plants, at the stage of overcoming When fruits ripen, an increase in hydrogen peroxide is observed, this maturity being able to be delayed by inhibition of hydrogen peroxide (PAULS K THOMPSON J- Plant Physiol. 1984 75 1152)

  In smokers, the activity of macrophages increasing, causes an abnormally high production of oxygenated water. Also a deficiency in catalase or peroxidases (anti-peroxidizing enzymes) can lead to serious medical conditions.

  Today, we know that there is an oxidative diet that it is imperative to fight thanks to the anti-radical and anti-lipoperoxid properties of vegetables and fruits. (patents NO 97 07273 and NO 99 11752) As for the destruction of hydrogen peroxide (H202) apart from specific enzymes, and especially glutathione, it has never been described until now, that food was able to break it down.

m -2

  It has been observed, which is surprising, that certain extracts of vegetables and edible fruits were endowed with a destructive activity of hydrogen peroxide (H202) similar to that of enzymes: peroxidases and catalase, leading to the formation of water and molecular oxygen.

  These plant food extracts have been the subject of work highlighting their anti-free radical properties and particularly their anti-lipoperoxid properties. The middle

  reaction of these activities being lipophilic (oxidation of fatty substances) In the context of the invention, the anti-hydrogen peroxide activity is carried out in a hydrophilic medium.

  The object of the present invention is to produce plant food extracts from

  OJ vegetables or fruits characterized by their anti-hydrogen peroxide activity.

  Here are some examples of the anti-hydrogen peroxide capacities, for 1 g of fruit and vegetable extracts, expressed in micrograms of oxygen; the measurements being carried out by potentiometry.

  Vegetables and Fruit Micrograms of oxygen eliminated Broccoli cabbage 4,800 Peas 3,754 Endive 3,600 Basil 2,813 Onion 2,695 Parsley 2,481 Tomato 2,447 Mas 2084 Green bean 1,855 Strawberry 1,600 Carrot 1,582 Leek 1,543 Orange 658 Pear 310 Extract from a Bordeaux wine 5,200 The present invention has for object the production of extracts intended to be used as

  food supplements, but also for skin application in the fields of cosmetics, human and animal dermatology.

  In the context of the invention, after having carried out measurements of hydrogen antiperoxide activity, the extracts of vegetables and fruits, of which mixtures can be made, will be chosen from those giving the highest results. for the elimination of this type

  active O2 In this case, the activity of active oxygen is systematically inhibited. The number of vegetables or fruits capable of being endowed with high anti-hydrogen peroxide properties is thus limited.

  -3 In order to combat exogenous peroxides, more particularly of food origin, but mainly endogenous hydrogen peroxide, the extracts of vegetables and fruits will be chosen according to their anti-hydrogen peroxide capacities. They will be used as food supplements, in the form of capsules, tablets, capsules, in aqueous solution

for example.

  The extracts of vegetables and fruits characterized by their anti-oxidant action (H202) will advantageously be incorporated into preparations intended to be applied to the skin to protect it from the aggressive effects of solar radiation or any other aggression triggering an inflammatory oxidative process, by activation phagocytic cells, producing IO of oxygenated water (wounds, burns, microbial action)

  Thus, for food supplements, we will use, for example, extracts such as Broccoli, endowed with a strong anti-hydrogen peroxide capacity, while in the context of skin application , we will use for example extracts like those, pea or endive.

  I5 It is well known that solar radiation is aggressive to the skin, causing

  the alteration of the connective tissue and therefore an activation of the neutrophils, producers, of the superoxide anion and of oxygenated water. This shows the advantage of using vegetable and fruit extracts in cosmetic preparations against premature aging of the skin.

  Non-limiting examples of compositions containing extracts of vegetables and fruits destructive of hydrogen peroxide, intended for the production of food supplements or for skin application. For food supplements, extracts are introduced to obtain a minimum anti-hydrogen peroxide capacity of 100 tg / 02 per dose. The extracts used in skin application are introduced at the minimum concentration of

  2 5 1 OOig / O2 of anti-hydrogen peroxide capacity per gram of emulsion.

  In Food Supplements -In the form of capsules of 200 mg Broccoli extract 42 mg Anti-peroxide capacity: 200 J'g / 02 -In the form of tablets of 200 mg Endive extract: 55 mg Anti-peroxide capacity: 200 pig / O2 -4 In Skin Application 1) Excipient ...................... 95g Endive extract .............. 5g (eliminating 3600 kg / 02 per g) Or per g of emulsion, a destructive capacity of peroxide oxygen of 180g 02 2) Excipient. .94g Pea extract. 5g (eliminating 3754 ktg / 02 per g) Mas extract. 7g (eliminating 2084 ptg / 02 per g) Or a destructive capacity of peroxidic oxygen of 188> g / 02 and 146kg / 02 per g of cream -5

Claims (3)

  1- Use of plant extracts, made from edible fruits and vegetables, characterized by their destructive activity of hydrogen peroxide (H202) similar to that of enzymes: Peroxidases and catalase, leading to the formation of water and d molecular oxygen. The anti-hydrogen peroxide activity is carried out in a hydrophilic medium. 2Use of plant extracts according to claim 1, characterized in that they are   selected according to their destructive capacity of hydrogen peroxide expressed in micrograms of oxygen per gram of extract.   3- Use of plant extracts according to claims 1 and 2, as supplements   I0 food, presented in the form of capsules, tablets, capsules or in aqueous solution.   4- Use of plant extracts according to claims 1 and 2, for application   cutaneous, in order to protect the skin from the aggressive effects of solar radiation or any other aggression triggering an inflammatory oxidative process by activation of   I5 phagocytic cells producing hydrogen peroxide.   - Use of plant extracts according to claims 1,2 and 4 in the preparation of   cosmetic, dermatological and veterinary products.