FR2822380A1 - Preventing adhesion of microbes to skin and mucosa, useful in cosmetics and for treating e.g. acne, using mixture of metal ions, hydrogencarbonate and o-diphenol - Google Patents

Abstract

Use of a mixture (A) containing manganese(II) and/or zinc(II) compounds; a hydrogencarbonate (I) and at least one o-diphenol (II) in (or to prepare) a composition for modifying adhesion of microorganisms to the skin and/or mucosa.

Description

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The invention relates to the use of a mixture comprising Mn (II) and / or Zn (II) in combination with hydrogencarbonate and at least one ortho-diphenol in or for the preparation of a composition, as an agent decreasing the adhesion of microorganisms, particularly bacteria, on the skin and / or the mucous membranes. In particular the compositions of the invention are intended to promote the imination of bad body odors or to fight against all cutaneous infections involving microorganisms, such as for example acne and / or dandruff.

 It is well known that the skin is covered with a flora responsible for a whole series of inconveniences ranging from the simple production of odor to more or less severe pathologies such as acne and / or dandruff.

 Commensal microorganisms living on or in the skin may be part of a resident (normal) microflora that is transient. Resident organisms normally grow on or in the skin. Their presence is established in well-defined distribution profiles. Temporarily present microorganisms are called transients. Usually, these organisms do not attach themselves firmly; they are unable to multiply and normally die after a few hours.

 The anatomy and physiology of the skin varies from one part of the body to another and the resident microflora reflects these variations.

 Most skin bacteria are present on superficial squamous epidermis, colonizing dead cells or closely associated with sebaceous and sweat glands. Excretions from these glands provide water, amino acids, urea, electrolytes, and specific fatty acids as nutrients primarily for Staphylococcus epidermidis and aerobic corynebacteria.

Gram-negative bacteria are generally present in wetter regions.

Yeasts Pityrosporum ovale and Pityrosporum orbiculare are normally present on the epicranium.

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Some dermatophytic fungi can colonize the skin and cause fungal infections, such as athlete's foot and ringworm.

ailleurs. De la même manière, Clostridium perfringens colonise habituellement le périnée et les cuisses, particulièrement chez les patients souffrant du diabète. Some pathogens present on or in the skin are transitory residents colonizing the areas around the orifices. Staphylococcus aureus is the best example. 11 ast present in the nostrils and perianal region but survives poorly

elsewhere. In the same way, Clostridium perfringens usually colonizes the perineum and thighs, particularly in patients with diabetes.

 Theoretically, the epidermis is not a favorable environment for colonization by microorganisms. Several factors such as the periodic drying of the skin, the slightly acidic pH of the skin, the high concentration of sodium chloride in sweat, certain natural inhibitory substances (bactericidal and / or bacteriostatic) are responsible for this hostile micro-environment.

 The absence of moisture induces dormancy in many residents of the microflora. However, on some parts of the body (epicranium, ears, axillary regions, genitourinary and anal regions, perineum and palms), the humidity is high enough to allow the existence of resident microflora .

 The acid pH (4-6) of the skin, due to the organic acids produced by staphylococci and sebaceous and sweat gland secretions, discourages colonization by many microorganisms.

 Sweat contains sodium chloride in a concentration that establishes hyperosmotic conditions on the surface of the skin and osmotically weighs on most microorganisms.

 Finally, some natural inhibitory substances help control colonization, overgrowth, and skin surface infection by resident microorganisms. For example, sweat glands excrete

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lysozyme qui Iyse Staphylococcus epidermidis et d'autres bactéries Gram-positives.

Lysozyme that lyse Staphylococcus epidermidis and other Gram-positive bacteria.

Some Gram-positive bacteria (Propionibacterium acnes) can change lipids secreted by the sebaceous glands into unsaturated fatty acids, such as oleic acid, which have strong antimicrobial activity on Gram-negative bacteria and fungi.

However, under certain conditions this natural defense system can, while being effective, present inconvenience or even be in default.

For example, certain natural inhibitory substances (bactericidal and / or bacteriostatic) due to the partial degradation of complex lipids secreted by the sweat glands are volatile and can be associated with a strong odor that is customary to fight. Certainly, many deodorants contain antibacterial substances that selectively act on gram-positive bacteria responsible for these degradations to reduce the production of unsaturated aromatic fatty acids and body odor. But deodorants can alter the microflora, mainly to gram-negative bacteria, and trigger infections as a result.

 It remains interesting to be able to dispose in the context of the treatment of body odors compounds and / or effective compositions and having no side effects.

 Another example is Propionibacterium acnes, the most commonly associated bacterium with cutaneous glands that is a Gram-positive, anaerobic and lipophilic rod. Bacteria caterpillar is usually harmless. However, it has been associated with a skin disease, juvenile acne. Acne usually appears during adolescence when the endocrine system is very active. Hormonal activity stimulates the overproduction of sebum, a fluid secreted by the sebaceous glands. A large volume of sebum accumulates in the glands and provides an ideal microenvironment for Propionibacterium acnes. In some individuals, this accumulation triggers an inflammatory response

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provoquant une rougeur et un gonflement du canal glandulaire et produisant un comédon, un bouchon de sébum et de kératine dans le canal. 11 en résulte des lésions inflammatoires (papules, pustules, nodules), communément appelées"points noirs".

causing redness and swelling of the glandular canal and producing a comedone, a plug of sebum and keratin in the canal. This results in inflammatory lesions (papules, pustules, nodules), commonly known as "blackheads".

Propionibacterium acnes seems to be the organism producing lipases that break down triglycerides of sebum into free fatty acids. These derivatives are particularly irritating because they can penetrate the dermis and promote inflammation.

 Yeasts Pityrosporum ovale and Pityrosporum orbiculare are commonly associated with the formation of dandruff.

 Strains of Staphylococcus aureus are known as super antigens, which promotes the appearance of irritation reactions and inflammatory processes.

 Of course, if necessary, it has long been known to use compounds such as antibiotics or certain natural or synthetic derivatives of vitamin A to overcome the deficiencies of this natural defense system.

 But, again, it is known that the use of such compounds has significant negative aspects. The misuse of antibiotics can lead to the emergence of resistant microorganisms over which they are no longer effective.

As for derivatives of vitamin A, it is known that they generally have significant side effects, which makes their use delicate.

 Thus, again, there is a need for compounds and / or compositions effective and having no side effects.

 Moreover, it is known that one of the key elements for a pathogen to induce an infectious disease is that it must be able to adhere to the host that it will colonize and invade.

et les tissus de l'hôte. La colonisation dépend de la capacité qu'a le germe pathogène à concurrencer avec succès la microflore normale de l'hôte pour les éléments nutritifs essentiels. Des structures spécialisées, permettant d'entrer en concurrence pour Indeed, after being transmitted to a suitable host, the pathogen must be able to bind and colonize the cells

and the tissues of the host. Colonization depends on the ability of the pathogen to successfully compete with the normal host microflora for essential nutrients. Specialized structures, allowing to compete for

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des sites d'attachement en surface, sont également nécessaires à la colonisation.

sites of attachment on the surface, are also necessary for colonization.

 Pathogenic organisms, like many non-pathogens, attach themselves very specifically to particular tissues. Adhesion structures are one of the elements of this specificity. They are specialized and present on the surface of the pathogen that bind to receptors specific host cells.

 It is therefore understood that one of the possible ways to treat the discomfort and / or skin infections is to fight against the adhesion of microorganisms to the cells of the skin and / or mucous membranes.

 Surprisingly and unexpectedly, the Applicant has discovered that a mixture comprising Mn (11) and / or Zn (11) in combination with hydrogen carbonate and at least one orthodiphenol has the particularity of modifying the attachment of microorganisms. on cells especially on skin cells and / or mucous membranes.

j'adhesion des micro-organismes sur la peau etlou les muqueuses. The subject of the invention is therefore the use, as active principle, in or for the preparation of a composition, of an effective amount of at least one such mixture as defined above, this mixture or the composition being intended to modify

I join microorganisms on the skin and / or mucous membranes.

 The composition is preferably intended for cosmetic or dermatological use, advantageously cosmetic.

 By active ingredient is meant any molecule or composition capable of modifying or modulating the operation of at least one biological system.

 By "modifying the adhesion of micro-organisms" he meant the ability to prevent, totally or partially, the adhesion of micro-organisms, or the curative capacity to facilitate the detachment of micro-organisms.

 Thus, the subject of the invention is the use, as active ingredient, in or for the preparation of a composition of an effective amount of at least one mixture as defined above,

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ce mélange ou la composition étant destiné à prévenir, totalement ou partiellement, I'adhésion des micro-organismes sur la peau et/ou les muqueuses.

this mixture or the composition being intended to prevent, totally or partially, adhesion of microorganisms on the skin and / or the mucous membranes.

ce mélange ou la composition étant destiné à faciliter le détachement des micro-organismes de la peau et/ou les muqueuses. Similarly, the subject of the invention is the use, as active ingredient, in or for the preparation of a composition of an effective amount of at least one mixture as defined above,

this mixture or the composition being intended to facilitate the detachment of microorganisms from the skin and / or the mucous membranes.

 In particular, the mixture or the composition containing it is used according to the invention for topical application to the skin and / or the mucous membranes.

 We have seen previously that the adhesion of microorganisms to the skin and / or mucous membranes has consequences that range from simple inconvenience (the smell) to more or less serious diseases.

 One of the aspects of the invention is thus to propose the use of at least one mixture as defined above as an active ingredient in or for the preparation of a composition, this mixture or the composition being intended for the care personal hygiene, including intimate care.

de les maintenir en bon état, d'en modifier l'aspect, de les parfumer ou d'en corriger l'odeur. Body care means any substance or preparation intended to be brought into contact with the various superficial parts of the human body and / or with the teeth and / or the mucous membranes in order to clean them, to protect them,

to keep them in good condition, to modify their appearance, to perfume them or to correct their odor.

 In particular, the subject of the invention is the use of at least one mixture as defined above as an active ingredient in or for the preparation of a composition, this mixture or the composition being intended to reduce bad odors. body.

 We have seen previously that the bacterial flora of the surface of the skin is responsible for a large number of disorders.

 Thus, the invention also relates to the use of at least one mixture as defined above as an active ingredient in or

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pour la préparation d'une composition, ce mélange ou la composition étant destiné à lutter contre les mycoses, I'acné, particulièrement l'acné juvénile et/ou les pellicules.

for the preparation of a composition, this mixture or the composition being intended to combat fungal infections, acne, especially juvenile acne and / or dandruff.

composition étant destiné ä lutter contre l'acné et/ou les pellicules. In particular, the subject of the invention is the use of at least one mixture as defined above as an active ingredient in or for the preparation of a composition, this mixture or the

composition for controlling acne and / or dandruff.

According to the invention, the molar concentration of Mn (II), Zn (II), or Mn (h) + Zn (H) in the final composition generally varies from 10-3 to 10 mM / 1, preferably from 10 to 10 mM. 2 to 1 MM / 1.

When using only Mn (11), the molar concentration of Mn (II) in the final composition is typically 10-3 to 10 mM, preferably 10-2 to 10-1 mM / l.

Preferably, when using only Zn (!!), The concentration of Zn (II) in the final composition is 5. 10-2 to 10 mM /), more preferably 5. 10-1 to 1 mM / 1 .

 The Mn (II) and / or Zn (II) of the mixture generally originates from a silica and / or an oxide of Mu (11) or Zn (II).

 Among the Mn (II) and Zn (II) salts suitable for the present invention, there may be mentioned chloride, fluoride, iodide, sulfate, phosphate, nitrate and perchlorate, carboxylic acid salts and mixtures thereof.

de Mn (il), t'acetate de Mn (ll) tétrahydraté, le lactate de Mn (H) trihydrat, le phosphate de Mn (il), le perchlorate de Mn (tut) tetrahydrat et le sulfate de Mn (lI) monohydrat. By way of example, mention may be made of manganese chloride, manganese carbonate (for example rhodochrosite), difluoride

Mn (II), Mn (II) acetate tetrahydrate, Mn (H) trihydrate lactate, Mn (II) phosphate, Mn (tet) tetrahydrate perchlorate and Mn (II) sulfate monohydrate .

Particularly preferred gels sani MnCI2 and Zoos.

 The carboxylic acid salts also include hydroxyl carboxylic acid salts such as gluconate.

 The hydrogencarbonate generally comes from an alkaline or alkaline earth hydrogencarbonate.

 Among the alkaline and alkaline-earth hydrogenocarbonates, mention may be made of the Na, K, Mg, Ca hdyrogenocarbonates and mixtures thereof, preferentially Na hdyrogenocarbonate.

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According to the invention, the molar concentrations of Mn (II), Zn (II) and HCO3 in the final mixture or composition are such that:

où [Mn ( !))], [Zn ()))] et [HCO3] représentent respectivement les concentrations molaires en Mn (li), Zn (ll) et HCO3 dans la composition.

where [Mn (!))], [Zn ())]] and [HCO3] respectively represent the molar concentrations of Mn (Li), Zn (II) and HCO3 in the composition.

Generally, the ratio ['- varies from 10-5 to 10', from [HCOg] preferably from 10-3 to 10-2 and is typically of the order of 5. 10-3.

In the case of Zn (!!), The ratio [Zn "is in general of an IHCO31 order 10 to 100 times higher than the ratio in the case of Mn (II).

Typically, this ratio is 10-4 or more, preferably 10-3 or higher, and preferably of the order of 5. 10-1.

In the Gas of a mixture of Mn (II) and Zn (Li), the ratio generally varies from 10-5 to 10-1, preferably 10-3 to 10-2, this ratio being chosen higher when the proportion Zn (II) in the Si mixture increases.

Generally, the molar concentration of Mn (Li), Zn (II) or Mn (II) + Zn (H) in the final composition ranges from 10-3 to 10 mM / preferably from 10 to 1 MM /.

The ortho-diphenols of the mixtures of the invention may be represented by formula (I):

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4 dans laquelle les substituants R1 ä R4, identiques ou différents, représentent un atome d'hydrogène, un radical halogène, hydroxyle, carboxyle, carboxylate d'alkyle, amino éventuellement substitué, alkyle linéaire ou ramifié éventuellement substitué, alcényle linéaire ou ramifié éventuellement substitué, cycloalkyle éventuellement substitué, alcoxy, alcoxyalkyle, alcoxyaryle, le groupe aryle pouvant être éventuellement substitué, aryle, aryle substitué, un radical hétéroxyclique éventuellement substitué, un radical contenant un ou plusieurs atomes de silicium, où deux des substituants R1 R R4 forment conjointement un cycle saturé ou insaturé contenant éventuellement un ou plusieurs hétéroatomes et éventuellement condensé avec un ou plusieurs cycles saturés ou insaturés contenant éventuellement un ou plusieurs hétéroatomes.

Wherein the substituents R 1 to R 4, which may be identical or different, represent a hydrogen atom, a halogen, hydroxyl, carboxyl, alkyl carboxylate, optionally substituted amino, optionally substituted linear or branched alkyl radical, optionally substituted linear or branched alkenyl radical; , optionally substituted cycloalkyl, alkoxy, alkoxyalkyl, alkoxyaryl, the optionally substituted aryl group, aryl, substituted aryl, an optionally substituted heteroxyclic radical, a radical containing one or more silicon atoms, wherein two of the R 1 R 4 substituents together form a saturated or unsaturated ring optionally containing one or more heteroatoms and optionally condensed with one or more saturated or unsaturated rings optionally containing one or more heteroatoms.

 The saturated or unsaturated cycles, optionally condensed, may also be optionally substituted.

 The alkyl radicals are generally the C1-C10 alkyl radicals, preferably the C1-C6 alkyl radicals, such as methyl, ethyl, propyl, butyl, pentyl and hexyl.

 The alkoxy radicals are in general C 1 -C 20 alkoxy radicals, such as methoxy, ethoxy, propoxy and butoxy.

a ! coxy (Ci-Czo) a ! kyte (Ci-C2o), te ! s que méthoxyméthyle, éthoxyméthyle, méthoxyéthyle, éthoxyéthyle, etc. Alkoxy radicals are preferably radicals

at ! coxy (Ci-Czo) a! kyte (Ci-C2o), te! Methoxymethyl, ethoxymethyl, methoxyethyl, ethoxyethyl, etc.

 The cycloalkyl radicals are in general the C 4 -C 8 cycloalkyl radicals, preferably the cyclopentyl and cyclohexyl radicals. The cycloalkyl radicals may be substituted cycloalkyl radicals, in particular with alkyl, alkoxy, carboxylic acid, hydroxyl, amine and ketone groups.

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The alkenyl radicals are preferably C1-C20 radicals such as ethylene, propylene, butylene, pentylene, methyl-2-propylene and decylene.

The radicals containing one or more silicon atoms are preferably polydimethylsiloxane, polydiphenylsiloxane, polydimethylphenylsiloxane and steroxydimethicone radicals.

The heterocyclic radicals are in general radicals comprising one or more heteroatoms chosen from O, N and S, preferably O or N, optionally substituted with one or more alkyl, alkoxy, carboxylic acid, hydroxyl, amine or ketone groups.

Among the preferred heterocyclic radicals, mention may be made of furyl, pyranyl, pyrrolyl, imidazolyl, pyrazolyl, pyridyl and thienyl groups.

More preferably, the heterocyclic groups are fused groups such as benzofuranyl, chromenyl, xanthenyl, indolyl, isoindolyl, quinolyl, isoquinolyl, chromannyl, isochromannyl, indolinyl, isoindolinyl, coumarinyl, isocoumarinyl, these groups may be substituted, in particular by one or more OH groups.

The preferred orthodiphenols are: - flavanols, catechin and epichatechin gallate, - flavonols such as quercetin, - anthocyanidins such as peonidine, - anthocyanins, for example oenine, - hydroxybenzoates, e.g. gallic acid, flavones such as luteolin, iridoids such as oleuropein, these products may be osylated (for example glucosylated) and lau in the form of oligomers (procyanidines); hydroxystilbenes, for example tetrahydroxy-3,3 ', 4,5'-stilbene, optionally osylated (for example glucosylated); 3,4-dihydroxyphenylalanine and its derivatives;

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- la 2, 3-dihydroxyphenylalanine et ses dérivés ; - la 4, 5-dihydroxyphenylalanine et ses dérivés ; ces dérivés pouvant être de la forme L (L-DOPA) ou D (D-DOPA), -le 4, 5-dihydroxyindole et ses dérivés ;

- le 5, 6-dihydroxyindole et ses dérivés ; -le 6, 7-dihydroxyindole et ses dérivés ; -le 2, 3-dihydroxyindole et ses dérivés ; - les dihydroxycinnnamates tels que !'acide caféique et l'acide chlorogénique ; - tes hydroxycoumarines ; - les hydroxyisocoumarines ; -les hydroxycoumarones ; - les hydroxyisocoumarines ; ; - les hydroxychalcones ; - les hydroxychromones ; -lesanthocyanes -les quinones ; - les hydroxyxanthones ; et - leg mélanges de ceux-ci.

2,3-dihydroxyphenylalanine and its derivatives; 4,5-dihydroxyphenylalanine and its derivatives; these derivatives may be of the form L (L-DOPA) or D (D-DOPA), -5,5-dihydroxyindole and its derivatives;

- 5,6-dihydroxyindole and its derivatives; 6,7-dihydroxyindole and its derivatives; 2,3-dihydroxyindole and its derivatives; dihydroxycinnnamates such as caffeic acid and chlorogenic acid; - your hydroxycoumarines; hydroxyisocoumarines; hydroxycoumarones; hydroxyisocoumarines; ; hydroxychalcones; - hydroxychromones; the anthocyanins; the quinones; hydroxyxanthones; and - mixtures of these.

 Where ortho-diphenols have D and L forms, both forms may be used in the compositions according to the invention.

 Orthodiphenols can be extracts of plants, fruits, citrus fruits, vegetables and mixtures of these extracts which contain many polyphenols as defined above.

rose et de thé. Among the plant extracts, mention may be made of the extracts of

rose and tea.

 Examples of fruit extracts include apple, grape (especially grape seed) and banana extracts.

 Among the vegetable extracts, mention may be made of potato extract.

 Mixtures of plant and / or fruit extracts such as mixtures of apple and tea extracts and mixtures of grape and apple extracts may also be used.

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Orthodiphenous! prefere is) a catechine.

The amount of orthodiphenol in the final composition can vary widely.

 In general, the amount of orthodiphenol in the final composition is at least 10 micromoles per milliliter of the final blend or composition.

 The compositions according to the invention may also contain an effective amount of at least one amino acid comprising at least one thiol group (SH) and preferably only one thiol group, these amino acids possibly being in the form of hydrochlorides.

 The amino acids that are preferred according to the invention are the amino acids which contain an amine function in position a with respect to a carboxylic acid function.

dans laquelle-R est un radical hydrocarboné divalent, linéaire ou ramifié, par exemple en C1-C10, de préférence en Ci-Ce, tel qu'un radical méthylène,

éthylène, butylène, éthylidène, propylidène, un radical cyclique saturé divalent, éventuellement substitué, par exemple en C4-C8, un groupe aromatique divalent, éventuellement substitué, tel qu'un radical phénylène, tolylène, xylylène. The preferred amino acids may be represented by formula (II):

in which-R is a divalent hydrocarbon radical, linear or branched, for example C 1 -C 10, preferably C 1 -C 6, such as a methylene radical,

ethylene, butylene, ethylidene, propylidene, a divalent saturated cyclic radical, optionally substituted, for example C 4 -C 8, an optionally substituted divalent aromatic group, such as a phenylene, tolylene, xylylene radical.

 Preferred amino acids for the compositions of the invention include cysteine and its derivatives, particularly L-cysteine and L-cysteine hydrochloride, and glutathione and its derivatives.

 The relative proportions of amino acid and oxidation dye precursor in the compositions of the invention may vary widely depending on the desired color. Generally, the amino acid / dye precursor molar ratio will range from 0.001 to 50, preferably from 0.01 to 5, and most preferably from 0.05 to 2.5.

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In general, the thiol amino acid content in the final composition is at least 0.01 micromoles per milliliter, preferably at least 0.1 micromoles / ml.

 By varying the nature of the dye precursors and amino acids of the composition and the relative proportion of amino acid and dye precursor, a variety of hues and in particular shades close to those of the natural tan can be obtained.

 Thus, the combination of cysteine and dihydroyxindole provides a brown shade, closer to that of a natural tan than the use of only dihydroxyindole which leads to a color only black.

 This association makes it possible to have a natural coloration of the skin and the hair which is a mixture of eumelanin and pheomelanin in variable proportions.

 The constituents of the mixture according to the invention can be introduced into the composition directly or in the form of solutions in a suitable solvent or mixture of solvents.

 Among the solvents suitable for the mixture according to the invention, mention may be made of water, alcohols, polar solvents and mixtures thereof.

 The alcohols are preferably lower (C1-C6) alkanols such as ethanol and isopropanol and alkanediols such as ethylene glycol, propylene glycol and pentane diol.

 Among the polar solvents, mention may be made of ethers, esters (in particular acetates), dimethylsulfoxide (DMSO), N-methylpyrrolidone (NMP), ketones (in particular acetone) and mixtures thereof.

eau/éthanol. The solvent preferably comprises water (in particular distilled or permuted) or a water / alcohol mixture, in particular

water / ethanol.

The amount of alcohol in the water / alcohol mixture may be up to 80% by weight of the water-alcohol mixture, preferably 1 to 50% by weight and more preferably 5 to 20% by weight.

 The subject of the invention is also a cosmetic process for treating disorders related to the adhesion of microorganisms consisting in applying a cosmetic composition to the skin.

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comprenant une quantité effective d'un mélange selon l'invention dans un milieu cosmétiquement acceptable.

comprising an effective amount of a mixture according to the invention in a cosmetically acceptable medium.

 By cosmetically acceptable medium is meant compatible with the skin, the scalp mucous membranes, nails, and hair.

 It may be in all the galenical terms normally used for topical application, especially in the form of an aqueous, hydroalcoholic or oily solution, an oil-in-water or water-in-oil or multiple emulsion, a aqueous or oily gel, a liquid anhydrous, pasty or solid product, an oil dispersion in an azide aqueous phase of spherules, these spherules may be polymeric nanoparticles such as nanospheres and nanocapsules or better lipid vesicles of ionic and / or nonionic type.

 This composition may be more or less fluid and have the appearance of a white or colored cream, an ointment, a milk, a lotion, a serum, a paste, a mousse . It can optionally be applied to the skin in the form of an aerosol.

It can also be in solid form, and for example in the form of a stick. It can be used as a care product, as a cleaning product, as a makeup product or as a simple deodorant product.

mélange selon l'invention. Thus, the subject of the invention is a cosmetic care, cleaning, makeup or deodorant composition comprising a

mixture according to the invention.

 In particular, the subject of the invention is a deodorant cosmetic composition comprising a mixture according to the invention.

 Even more particularly, the deodorant composition of the invention is in the form of a stick.

 In the case where a coloration of the skin and / or the hair is not desirable, the composition will contain a formulation avoiding penetration into the stratum corneum or the hair fiber. Orthodiphenols which form weakly or non-colored compounds, for example, caffeic acid, will preferably be used.

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acryliques tals qua las copolymères d'acrylates/alkylacrylates, las polyacrylamides, les polysaccharides, les gommes naturelles et les argiles, et, comme gélifiants lipophiles, on peut citer les argiles modifiées comme les bentones, les gels métalliques d'acides gras, la silice hydrophobe et les polyéthylènes.

acrylics, acrylates / alkyl acrylates, polyacrylamides, polysaccharides, natural gums and clays, and, as lipophilic gelling agents, modified clays such as bentones, metal gels of fatty acids, silica, hydrophobic and polyethylenes.

 As active agents, it is possible to use, in particular, moisturizers such as polyols (for example glycerin), vitamins (for example D-panthenol), anti-inflammatory agents, soothing agents (allantoin, cornflower water), filters UVA and UVB, matting agents (eg partially crosslinked polydimethylorganosiloxanes sold under the name KSG & comm. By Shin Etsu), and mixtures thereof.

produits tenseurs tels que les protéines végétales et leurs hydrolysas, en particulier l'extrait de protéine de soja vendue sous le nom d'Eleseryl (D par la sociätä LSN ou le dérivé d'avoine vendu sous la dénomination Reductine&commat; par la société Silab. It is also possible to add anti-wrinkle

tensor products such as vegetable proteins and their hydrolysas, in particular the soy protein extract sold under the name Eleseryl® (D) by the company LSN or the oat derivative sold under the name Reductin® by the company Silab.

The compositions according to the invention may be in various forms, such as in the form of liquids, creams, gels, or in any other suitable form.

According to a first embodiment, the compositions according to the invention may be packaged in the form of a single-compartment aerosol containing all the ingredients of the composition and a conventional inert propellant gas such as nitrogen, a saturated hydrocarbon such as isopropane or a fluorinated hydrocarbon, for example a Freon &commat;.

t'un contenant Mn ( !)) et/ou Zn (lI) et l'orthodiphänol, et éventuellement le ou les acides aminés, I'autre renfermantl'hydrogénocarbonate, le contenu des deux compartiments étant mélangés ou appliqués successivement au moment de l'emploi. In a second embodiment, the composition according to the invention may be packaged in the form of a kit comprising two separate containers,

one containing Mn (!)) and / or Zn (lI) and the orthodiphenol, and optionally the amino acid (s), the other containing the hydrogencarbonate, the contents of the two compartments being mixed or applied successively at the time of l 'employment.

 In a third embodiment, the composition may be contained in a single chamber pump system, without air intake, or in a two compartment pump system, Mn (II) and / or Zn (H) and orthodiphenol being in a compartment and hydrogen carbonate in

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)'autre.

)'other.

Other features and advantages of the invention will emerge more clearly from the examples which follow and the appended figures, given for illustrative and non-limiting purposes. The proportions are given in weight percent.

EXAMPLE 1 Measurement of the activity of a mixture according to the invention on the adhesion of microorganisms on the skin or on the human mucous membranes
The activity of the mixture according to the invention on the adhesion of microorganisms on the skin or on the human mucosa is tested on a bacteria / skin adhesion model, as described below.

 This ex vitro bacteria / skin adhesion model is based on the use of intact human skin explants sandwiched between a bottomless 96-well plate and a glass plate. Before assembly, the hypodermis was removed from the skin and frozen at -80 ° C. After thawing, the skin fragment was dialysed in three baths of phosphate buffered saline (PSB) at 4 ° C. for three hours. The skin is mounted just before the test. To avoid drying out, the whole experience takes place with the skin immersed in a bath of PBS.

50 μl of a diluted bacterial suspension of Staphylococcus eperdermidis (ATCC 12228) is distributed on the skin in the sealed wells. After two hours of incubation at 20oC, the wells are washed three times with phosphate buffered saline (PBS) to remove non-adherent bacteria.

100 μl of the following solutions are distributed in the wells and incubated again for one hour at 20 ° C. The products and free bacteria are then removed and the wells are washed three times with PBS. Bacteria still adherent are detached from the skin with 100 μl of chaotropic guanidine 4 MIR buffer; 5 mM EDTA / in 50 mM Tris-HCl, pH 8.0).
The solutions used had the following compositions: 1. MnCl 2 solution at 1 mM / l in distilled water,

<Desc / Clms Page number 17>

 2. 100 mM / l NaHCO3 solution in distilled water; 3. Catechise solution at 100 mMII in ethanol; 4. Mixture according to the invention constitutes 180 μg of solution 1, 180 μl of solution 2 and 40 μl of solution 3.

 The positive control (guanidine) is also included in the study as a reference.

 20 μl of each sample of unhooked bacteria are transferred onto nitrocellulose membranes using a "dot-blot" matrix allowing subsequent densitometric quantification. The membranes are then saturated overnight at 4 ° C. with a PBS / 0 solution. 005% Tween (PBST) containing 1% by weight of skimmed milk. After washing with PBST, the bacteria are labeled with a streptavidin-peroxidase conjugate for 1 hour at 20 ° C. After extensive washing, the immobilized biotins were revealed by chemiluminescence on Kodak MP film. Image capture is then performed on Gel Print 2000i and densitometric measurements of the percentage of adherent bacteria are obtained using One-D-Scan software.

 The results are given in Table I below.

BOARD)

<Tb>
<tb> Control <SEP> Control <SEP> Solution <SEP> 1 <SEP> Solution <SEP> 2 <SEP> Solution <SEP> 3 <SEP> Blend <SEP> 4
<tb> positive <SEP> MnCl2 <SEP> naHCO3 <SEP> Catechin
<tb> (guanidine
<tb>)
<tb> Bacteria <SEP> 100 <SEP> 4 <SEP> 60 <SEP> 87 <SEP> 42 <SEP> 0
<tb> adherents <SEP> (p <0, <SEP> 01) <SEP>(p> 0, <SEP> 01) <SEP> (p <0, <SEP> 05) <SEP> (p <0 , <SEP> 01) <SEP> (p <0, <SEP> 01)
<tb>%
<Tb>
Control: PBS buffer alone Positive control: Guanidine guanidine 4M /; EDTA 5mM / l in TrisMCl 50 mM / l, pH 8,0-Stimulates the desorption of bacteria and inhibits adhesion.

 The results show that if the solution 1 (Mn) and the solution 3 (catechise) substantially reduce the adhesion of the bacteria, the mixture 4 according to the invention containing the three active ingredients, Mn, HCO3 and Catechin, makes the adhesion completely disappear. .

Claims (28)

1. Use in or for the preparation of a composition as an active ingredient of an effective amount of a mixture comprising Mn (11) and / or Zn (11) in combination with hydrogen carbonate and at least an orthodiphenol, the mixture or composition being intended to modify the adhesion of microorganisms on the skin and / or mucous membranes.  2. Use in or for the preparation of a composition as an active ingredient of an effective amount of a mixture comprising Mn (II) and / or Zn (II) in combination with hydrogencarbonate and at least one orthodiphenol, the mixture or the composition being intended to prevent, totally or partially, adhesion of microorganisms on the skin and / or mucous membranes.  3. Use in or for the preparation of a composition as an active ingredient of an effective amount of a mixture comprising M n (11) and / or Zn (11) in combination with hydrogencarbonate and less an orthodiphenol, this mixture or the composition being intended to facilitate the detachment of microorganisms from the skin and / or the mucous membranes.  4. Use according to any one of the preceding claims, characterized in that the mixture or the composition is used topically on the skin and / or mucous membranes.  5. Use in or for the preparation of a composition as an active ingredient of an effective amount of a mixture comprising Mn (11) and / or Zn (11) in combination with hydrogencarbonate and at least an orthodiphenol, this mixture or the composition being intended for personal hygiene care. <Desc / Clms Page number 19>

 6. Use in or for the preparation of a composition, as an active ingredient, of an effective amount of a mixture comprising Mn (II) and / or Zn (II) in combination with hydrogen carbonate and at least one an orthodiphenol, this mixture or the composition being intended for intimate hygiene care.  7. Use in or for the preparation of a composition, as an active ingredient of an effective amount, of a mixture comprising Mn (II) and / or Zn (II) in combination with hydrogencarbonate and at least an orthodiphenol, the mixture or composition being intended to reduce odors.  8. Use in or for the preparation of a composition, as an active ingredient, of an effective amount of a mixture comprising Mn (II) and / or Zn (II) in combination with hydrogencarbonate and at least orthodiphenol, a mixture or

 the composition being intended to combat fungal infections and / or achilles and / or dandruff.  9. Use according to the preceding claim, characterized in that the composition is intended to combat juvenile age.  10. Use according to any one of the preceding claims, characterized in that the molar concentrations [Mn () t)], [Zn (11)] and [HCOg] in e meiange or the final composition are such that:

<Desc / Clms Page number 20>

 where [Mn () i)], [Zn (i))] and [HCO3] respectively represent the molar concentrations of Mn (Li), Zn (II) and HCO3 in the mixture or composition.

11. Use according to claim 10, characterized in that the ratio '-'-. varies from 10-5 to 10-1, preferably from 10-3 to [HCO3] 10-2 and more preferably in the range of 5-10-3. 12. Use according to claim 10 or 11 characterized in that the ratio is at least 10-4, preferably [HCO3] of at least 10-3, and more preferably of the order of 5. 10- . 13. Use according to claim 10, characterized in that the ratio [Mn (H) + Zn (H)] varies from 10-5 to 10-1, preferably [HCO2] 10 'to 10'. 14. Use according to any one of the preceding claims, characterized in that orthodiphenol is a compound of formula (I):

 in which the substituents R1 to R4, which are identical or different, represent a hydrogen atom, a halogen radical,

<Desc / Clms Page number 21>  hydroxyl, carboxyl, alkyl carboxylate, optionally substituted amino, optionally substituted linear or branched alkyl, optionally substituted linear or branched alkenyl, optionally substituted cycloalkyl, alkoxy, alkoxyalkyl, alkoxyaryl, the optionally substituted aryl group, aryl, substituted aryl, an optionally substituted heteroxyclic radical, a radical containing one or more silicon atoms, wherein two of the substituents R1 to R4 together form a saturated or unsaturated ring optionally containing one or more heteroatoms and optionally condensed with one or more saturated or unsaturated rings optionally containing a or more heteroatoms.

 15. Use according to any one of claims 1 to 13, characterized in that Ilorthodiphenol is chosen from

 flavanols, flavonols, anthocyaninidines, anthocyanins, hydroxybenzoates, flavones, iridoids, compounds which may optionally be osylated and / or in the form of oligomers, optionally osylated hydroxystilbenes, 3,4-dihydroxyphenylalanine and its derivatives, 2,3-dihydroxyphenylalanine and its derivatives, 4,5-dihydroxyphenylalanine and its derivatives, 4,5-dihydroxyindole and its derivatives, 5,6-dihydroxyindole and its derivatives, 6,7-dihydroxyindole and its derivatives, 2-dihydroxyphenylalanine and its derivatives; , 3-dihydroxyindole and its derivatives, dihydroxycinnamates, hydroxycoumarines, hydroxyisocoumarines, hydroxycoumarones, hydroxyisocoumarones, hydroxychalcones, hydroxychromones, anthocyanins, quinones, hydroxyxantones, and mixtures of two or more of the foregoing compounds.  16. Use according to any one of claims 1 to 3, characterized in that the orthodiphenol is selected from extracts of plants, fruits, citrus fruits, vegetables and mixtures thereof. <Desc / Clms Page number 22>

17. Use according to claim 16, characterized in that orthodiphenol is selected from tea extracts, grape, apple, banana, potato and mixtures thereof.  18. Use according to any one of the preceding claims, characterized in that the orthodiphenol is catechin.  19. Use according to any one of the preceding claims, characterized in that orthodiphenol is present in an amount of at least 10 micromoles per milliliter of mixture or composition.  20. Use according to any one of the preceding claims, characterized in that the composition is in the form of an aqueous-alcoholic or oily aqueous solution.  21. Use according to any one of claims 1 to 15, characterized in that the composition is in the form of an oil-in-water emulsion or water-in-oil or multiple.  22. Use according to any one of claims 1 to 15, characterized in that the composition is in the form of an aqueous or oily gel.  23. Use according to any one of claims 1 to 19, characterized in that the composition is in the form of a liquid anhydrous product, pasty or solid.  24. Use according to any one of claims 1 to 19, characterized in that the composition is in the form of an oil dispersion in an aqueous phase using spherules. <Desc / Clms Page number 23>

25. Use according to any one of the preceding claims, characterized in that the composition contains, in addition, at least one adjuvant selected from hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic active agents, preservatives, antioxidants, solvents , perfumes, fillers, filters, pigments, chelating agents, odor absorbers, dyestuffs, matting agents and mixtures thereof.  26. Cosmetic treatment process for treating the disorders linked to the adhesion of microorganisms consisting in applying to the skin a cosmetic composition comprising at least one mixture of Mn (II) and / or Zn (II) in combination with hydrogencarbonate and at least one orthodiphenol in a cosmetically acceptable medium.  27. A deodorant cosmetic composition comprising an effective amount of a mixture comprising Mn (it) and / or Zn (II) in combination with hydrogencarbonate and at least one orthodiphenol, this mixture or the composition being intended to modify the adhesion microorganisms on the skin and / or mucous membranes.

28. A deodorant stick comprising an effective amount of a mixture comprising Mn (II) and / or Zn (it) in association with hydrogencarbonate and at least one orthodiphenol, said mixture or composition being intended to modify the adhesion of the microorganisms on the skin and / or mucous membranes.