FR2548685A1 - New multi-cell plate which can be used in particular for immunological and biological reactions - Google Patents

New multi-cell plate which can be used in particular for immunological and biological reactions Download PDF

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Publication number
FR2548685A1
FR2548685A1 FR8311151A FR8311151A FR2548685A1 FR 2548685 A1 FR2548685 A1 FR 2548685A1 FR 8311151 A FR8311151 A FR 8311151A FR 8311151 A FR8311151 A FR 8311151A FR 2548685 A1 FR2548685 A1 FR 2548685A1
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France
Prior art keywords
plate
tanks
tank
height
washing fluid
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Granted
Application number
FR8311151A
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French (fr)
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FR2548685B1 (en
Inventor
Gabriel Peltre
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Institut Pasteur de Lille
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Institut Pasteur de Lille
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Application filed by Institut Pasteur de Lille filed Critical Institut Pasteur de Lille
Priority to FR8311151A priority Critical patent/FR2548685B1/en
Publication of FR2548685A1 publication Critical patent/FR2548685A1/en
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Publication of FR2548685B1 publication Critical patent/FR2548685B1/en
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
    • G01N30/94—Development

Abstract

Multi-cell plate, whose cells are isolated from each other, which can be used for immunological and biological reaction. It comprises a plurality of flat-bottomed cells 2, 15, oblong in shape, which are parallel to each other, whose length is substantially equal to one of the dimensions of the plate 1, 8, the said cells being separated from each other by walls 6, 20 manufactured in one piece, whose thickness is sufficient to ensure the flatness and, where appropriate, the rigidity of the plate. Application to the simultaneous incubation and washing of a plurality of supports obtained from a physico-chemical separation operation such as electrophoresis or chromatography.

Description

La présente invention est relative à une nouvelle plaque à cuves multiples utilisable, en particulier, pour des réactions immunologiques ou biologiques, et notamment pour l'incubation et le lavage de bandelettes de papier ou de tout autre support provenant d'opérations de séparation physicochimiques telles que électrophorèse ou chromatographie. The present invention relates to a new multi-cell plate usable, in particular, for immunological or biological reactions, and in particular for the incubation and washing of strips of paper or of any other support originating from physicochemical separation operations such as than electrophoresis or chromatography.

Conformement à l'Art antérieur, on met en contact de telles bandelettes avec des volumes de liquide relativement faibles, dans des boîtes de Petri places sur un tambour rotatif à axe incliné par rapport à l'horizontale. Toutefois, cette solution nécessite une consommation importante de boîtes de Pétri dont l'encombrement est considerable, en sorte que le nombre de botes de Petri que peut porter un tambour est limite. De plus, le lavage de ces bandelettes ne peut pas se faire dans de bonnes conditions, attendu qu'elles ne permettent pas d'avoir une arrivee et une évacuation d'eau en continu. In accordance with the prior art, such strips are brought into contact with relatively small volumes of liquid, in Petri dishes placed on a rotary drum with an axis inclined relative to the horizontal. However, this solution requires a significant consumption of Petri dishes whose size is considerable, so that the number of Petri dishes that can carry a drum is limited. In addition, the washing of these strips can not be done in good conditions, since they do not allow to have a continuous arrival and discharge of water.

La presente invention s'est en consequence donne pour but de pourvoir à une nouvelle plaque à cuves multiples à usages biologiques, qui repond mieux aux necessites de la pratique que les moyens utilises jusqu'à pressent, notamment pour l'incubation et le lavage de bandelettes de papier, en particulier de papier filtre provenant de processus d'elec- trophorèse, notamment en ce qu'elle présente un faible encombrement et permet la realisation simultanee d'une pluralité de réactions biologiques ou immunologiques sur une plaque unique, dans des conditions d'etancheité optimales des cuves les unes par rapport aux autres ; - en ce qu'elle permet un lavage en continu et une evacuation en continu des fluides de lavage, - en ce qu'elle est conçue de manière à permettre la superposition par empilement d'une pluralité de plaques les unes sur les autres, augmentant ainsi le nombre de rections ralisables en l'espace d'un cycle de traitement donne, - en ce qu'elle peut être fabriquee en série, par moulage, à un prix de revient relativement bas. The present invention has therefore given itself the aim of providing a new plate with multiple tanks for biological uses, which responds better to the necessities of the practice than the means used until pressent, in particular for the incubation and the washing of strips of paper, in particular of filter paper coming from electrophoresis processes, in particular in that it has a small footprint and allows the simultaneous realization of a plurality of biological or immunological reactions on a single plate, under conditions optimal sealing of the tanks relative to each other; - in that it allows continuous washing and continuous evacuation of washing fluids, - in that it is designed so as to allow the superposition by stacking of a plurality of plates on each other, increasing thus the number of rections achievable in the space of a treatment cycle gives, - in that it can be manufactured in series, by molding, at a relatively low cost price.

La pressente invention a pour objet une plaque à cu ves multiples isolees les unes des autres, à usages biologiques, caracterisee en ce qu'elle comprend une pluralite de cuves de forme oblongue parallèles entre elles, dont la longueur est sensiblement égale à l'une des dimensions (longueur ou largeur) de la plaque et en ce que lesdites cuves sont se- parees les unes des autres par des parois venues de fabrication, dont l'epaisseur est suffisante pour assurer la plane te de la plaque et eventuellement la rigidite de celle-ci. The subject of the present invention is a plate with multiple cells isolated from each other, for biological uses, characterized in that it comprises a plurality of oblong-shaped tanks parallel to each other, the length of which is substantially equal to one dimensions (length or width) of the plate and in that said tanks are separated from each other by walls which have come from manufacture, the thickness of which is sufficient to ensure the plane of the plate and possibly the rigidity of this one.

Selon un mode de realisation avantageux de la plaque à cuves multiples conforme à l'invention, le fond des cuves est plat. According to an advantageous embodiment of the multi-cell plate according to the invention, the bottom of the cells is flat.

Selon encore un mode de réalisation avantageux de la plaque à cuves multiples conforme à l'invention, la plaque comporte entre les parois d'extremite des cuves et l'arete correspondante de la plaque, une plage plane propre à recevoir des indications d'identification de chacune des cuves. According to another advantageous embodiment of the multi-cell plate in accordance with the invention, the plate comprises between the end walls of the cells and the corresponding edge of the plate, a flat area suitable for receiving identification indications of each of the tanks.

Selon une disposition avantageuse de ce mode de realisation, ladite plage plane est délimitez entre une seule des extremités des cuves et l'arête correspondante de la plaque. According to an advantageous arrangement of this embodiment, said flat area is delimited between only one of the ends of the tanks and the corresponding edge of the plate.

Selon un mode de realisation avantageux de la plaque à cuves multiples conforme à l'invention, le rapport hauteur/largeur de chaque cuve est compris entre 2/1 et 2,5/1. According to an advantageous embodiment of the multi-cell plate according to the invention, the height / width ratio of each cell is between 2/1 and 2.5 / 1.

Selon un autre mode de~realisation avantageux de la plaque à cuves multiples conforme à l'invention, le rapport longeur/hauteur/largeur de chaque cuve est compris entre 16/2/1 et 18/2,5/1.  According to another advantageous embodiment of the multi-tank plate according to the invention, the length / height / width ratio of each tank is between 16/2/1 and 18 / 2.5 / 1.

Selon encore un autre mode de realisation avantageux de la plaque à cuves multiples conforme à l'invention, le rapport largeur de cuve/epaisseur de paroi longitudinale de chaque cuve est de l'ordre de 2,5/1 à 3,5/1.  According to yet another advantageous embodiment of the multi-tank plate according to the invention, the ratio of tank width / longitudinal wall thickness of each tank is of the order of 2.5 / 1 to 3.5 / 1 .

Selon un autre mode de réalisation avantageux de la plaque à cuves multiples conforme à l'invention, celle-ci est pourvue sur le pourtour de sa base d'un rebord propre à permettre son empilement sur une autre plaque identique. According to another advantageous embodiment of the multi-tank plate according to the invention, it is provided on the periphery of its base with a rim capable of stacking on another identical plate.

Conformement à l'invention, la plaque à cuves mul tiples est pourvue de moyens de raccordement avec un tuyau d'alimentation en fluide de lavage et de moyens d'evacuation du fluide de lavage. In accordance with the invention, the multiple tank plate is provided with connection means with a hose for supplying washing fluid and with means for discharging the washing fluid.

Selon une disposition avantageuse de ce mode de realisation, lesdits moyens de raccordement comprennent un orifice tube menage en partie haute de la plaque, connecte à un tuyau d'alimentation en fluide de lavage. According to an advantageous arrangement of this embodiment, said connection means comprise a tube orifice in the upper part of the plate, connected to a pipe for supplying washing fluid.

Selon une autre disposition avantageuse de ce mode de réalisation, lesdits moyens d'evacuation comprennent en partie basse de la plaque une collecte en pente à l'extremite inférieure de laquelle est branche un tuyau d'évacuation du fluide de lavage. According to another advantageous arrangement of this embodiment, said evacuation means comprise in the lower part of the plate a sloping collection at the lower end of which is connected a hose for evacuating the washing fluid.

Selon un mode de realisation avantageux de la plaque à cuves multiples conforme à l'invention, celle-ci comporte du côte de la plaque adjacent aux moyens de raccordement avec un tuyau d'alimentation en fluide de lavage, une zone reservoir à partir de laquelle le fluide de lavage se deverse dans les cuves. According to an advantageous embodiment of the multi-tank plate according to the invention, the latter comprises on the side of the plate adjacent to the connection means with a hose for supplying washing fluid, a reservoir zone from which the washing fluid spills into the tanks.

Selon une disposition avantageuse de ce mode de realisation, la zone reservoir est délimitée exterieurement par la paroi latérale externe correspondante de la plaque, et intérieurement par la paroi d'extrémité correspondante de chaque cuve, dont la hauteur est legèrement inférieure à la hauteur de la paroi latérale externe de la plaque afin de créer un trop-plein en direction des cuves, le fond du reser- voir étant formé par une entretoise qui relie les deux parois ci-dessus et qui est située à une hauteur sensiblement comprise entre le quart et le tiers de la hauteur de la paroi d'extremite des cuves. According to an advantageous arrangement of this embodiment, the reservoir zone is delimited externally by the corresponding external lateral wall of the plate, and internally by the corresponding end wall of each tank, the height of which is slightly less than the height of the external side wall of the plate in order to create an overflow in the direction of the tanks, the bottom of the tank being formed by a spacer which connects the two above walls and which is situated at a height substantially between a quarter and a third of the height of the end wall of the tanks.

Selon une modalite avantageuse de ce mode de realisation, les entretoises susdites reçoivent des indications d'identification de chacune des cuves. According to an advantageous modality of this embodiment, the above-mentioned spacers receive identification indications from each of the tanks.

Selon un mode de réalisation avantageux de la plaque à cuves multiples conforme à 11 invention, celle-ci comporte sur le pourtour de sa partie supérieure un decrochement pour la mise en place d'un couvercle de fermeture.  According to an advantageous embodiment of the plate with multiple tanks in accordance with the invention, it comprises on the periphery of its upper part a recess for the installation of a closing cover.

Outre les dispositions qui precèdent, l'invention comprend encore d'autres dispositions, qui ressortiront de la description qui va suivre. In addition to the foregoing provisions, the invention also comprises other provisions, which will emerge from the description which follows.

L'invention vise plus particulièrement les plaques à cuves multiples conformes aux dispositions qui précèdent, ainsi que les enceintes d'expérimentation dans lesquelles lesdites plaques sont incluses. The invention relates more particularly to multi-cell plates in accordance with the above provisions, as well as to the experimental chambers in which said plates are included.

L'invention sera mieux comprise à l'aide du complement de description qui va suivre, qui se refère aux dessins annexes dans lesquels
- la figure 1 est une vue en perspective d'un mode de realisation de la plaque à cuves multiples conforme à l'invention
- la figure 2 est une vue en coupe partielle pratiquee perpendiculairement à l'axe des cuves, du mode de realisation de la figure i
- la figure 3 est une vue en perspective d'un autre mode de realisation de la plaque à cuves multiples conforme à l'invention, dont
- la figure 4 est une vue en coupe partielle analogue à celle de la figure 2 ; ;
- la figure 5 est une vue en perspective d'un troisième mode de realisation de la plaque à cuves multiples conforme à l'invention, dont
- la figure 6 est une vue en coupe suivant VI-VI de la figure 5 et dont
- la figure 7 est une vue en coupe suivant VII-VII de la figure 5.The invention will be better understood using the additional description which follows, which refers to the accompanying drawings in which
- Figure 1 is a perspective view of an embodiment of the multi-tank plate according to the invention
- Figure 2 is a partial sectional view taken perpendicular to the axis of the tanks, of the embodiment of Figure i
- Figure 3 is a perspective view of another embodiment of the multi-tank plate according to the invention, of which
- Figure 4 is a partial sectional view similar to that of Figure 2; ;
- Figure 5 is a perspective view of a third embodiment of the multi-tank plate according to the invention, of which
- Figure 6 is a sectional view along VI-VI of Figure 5 and of which
- Figure 7 is a sectional view along VII-VII of Figure 5.

Il doit être bien entendu, toutefois, que ces dessins et les parties descriptives correspondantes, sont donnes uniquement à titre d'illustration de l'objet de l'invention, dont ils ne constituent en aucune-manière une limitation. It should be understood, however, that these drawings and the corresponding descriptive parts, are given solely by way of illustration of the subject of the invention, of which they do not in any way constitute a limitation.

La plaque conforme à l'invention designee d'une fa çon generale par la reference 1, comprend une pluralité de cuves 2 dont la longueur est sensiblement égale à l'une des dimensions de la plaque, dans l'exemple represente à la lar geur de la plaque 1, une zone plane 3 etant délimitée entre l'arete supérieure 4 de la plaque et les bords supérieures des parois d'extremite des cuves 2, pour recevoir des indications d'identification des cuves, telles que des numéros par exemple. The plate according to the invention generally designated by the reference 1, comprises a plurality of tanks 2 whose length is substantially equal to one of the dimensions of the plate, in the example represented by the width of the plate 1, a flat area 3 being delimited between the upper edge 4 of the plate and the upper edges of the end walls of the tanks 2, to receive indications of identification of the tanks, such as numbers for example.

Le fond 5 de chaque cuve 2 est plat, pour recevoir par exemple des bandelettes de papier-filtre provenant d'une electrophorèse, et est dimensionne pour recevoir lesdites bandelettes rigoureusement à plat. Les parois 6 de séparation des cuves 2 adjacentes sont dimensionnées de manière à isoler les cuves 2 les unes des autres et leur epaisseur est calcu leè de telle manière qu'elles jouent un rôle de rigidification de la plaque conforme à l'invention. The bottom 5 of each tank 2 is flat, for receiving, for example, strips of filter paper from an electrophoresis, and is dimensioned to receive said strips strictly flat. The walls 6 of separation of the adjacent tanks 2 are dimensioned so as to isolate the tanks 2 from each other and their thickness is calculated in such a way that they play a role of stiffening the plate according to the invention.

Selon un mode de realisation avantageux de l'invention, la plaque 1 peut être pourvue d'un rebord 7 (cf. figure 3) pour permettre son empilement sur des plaques identiques. According to an advantageous embodiment of the invention, the plate 1 can be provided with a flange 7 (cf. FIG. 3) to allow it to be stacked on identical plates.

Les cuves 2 de forme oblongue que comporte la plaque 1 sont de forme parallelepipedique rectangle ou bien elles peuvent être arrondies à leurs extremites pour offrir le minimum d'asperites et faciliter l'insertion et la sortie des bandelettes. The oblong-shaped tanks 2 that the plate 1 comprises have a rectangular parallelepipedal shape or else they can be rounded at their ends to offer the minimum of asperites and facilitate the insertion and exit of the strips.

La plaque 8 à cuves multiples representee aux figures 5 à 7 comprend une pluralite de cuves 15 parallèles jux taposees, isolees les unes des autres par des parois 20 elle comporte un orifice 9 tube destine à être raccordé à un tuyau 10 d'alimentation en fluide de lavage tel que de l'eau, et qui déverse ledit fluide dans un reservoir 11 forme par la paroi externe 12 de la plaque 8, la paroi d'extremite 13 correspondante de chaque cuve 15 et par une entretoise 14 qui relie les parois 12 et 13 entre elles. L'epaisseur de l'entretoise 14 est au moins egale à celle des parois 13, afin d'assurer une bonne rigidite à la plaque 8.Dans ce mode de realisation, ce sont avantageusement les entretoises 14 qui portent les indications d'identification des cuves. La paroi d'extremite 13 de chacune des cuves parallèles juxtaposees est de hauteur legèrement inferieure à celle de la paroi 12 de la plaque, creant ainsi un trop-plein par lequel le fluide de lavage penètre à partir du reservoir 11 dans les cuves 15 ainsi alimentees par ce reservoir.Ainsi le fluide de lavage alimente en continu par le tuyau 10 et l'orifice 9, penètre en continu dans le reservoir 11 et, de là, dans les cuves 15; il sort, de même, en continu des cuves 15 en raison du fait que la paroi d'extremite 16 de chaque cuve, situee à l'oppose de la paroi d'extremite 13, est de faible hauteur afin de permettre au fluide de lavage de se déverser à cette extremi- té de la cuve 15, dans la collecte 17 dont la pente facilite l'evacuation du fluide de lavage par le tuyau d'evacuation 18 raccorde à la collecte 17. The plate 8 with multiple tanks represented in FIGS. 5 to 7 comprises a plurality of tanks 15 parallel jux taposees, isolated from each other by walls 20 it comprises an orifice 9 tube intended to be connected to a pipe 10 for supplying fluid of washing such as water, and which pours said fluid into a reservoir 11 formed by the external wall 12 of the plate 8, the corresponding end wall 13 of each tank 15 and by a spacer 14 which connects the walls 12 and 13 between them. The thickness of the spacer 14 is at least equal to that of the walls 13, in order to ensure good rigidity to the plate 8. In this embodiment, it is advantageously the spacers 14 which bear the indications for identifying the tanks. The end wall 13 of each of the juxtaposed parallel tanks is slightly lower in height than that of the wall 12 of the plate, thus creating an overflow through which the washing fluid penetrates from the tank 11 into the tanks 15 thus supplied by this reservoir. Thus the washing fluid continuously feeds through the pipe 10 and the orifice 9, penetrates continuously into the tank 11 and, from there, into the tanks 15; it also leaves continuously from the tanks 15 owing to the fact that the end wall 16 of each tank, located opposite the end wall 13, is of low height in order to allow the washing fluid pouring out at this end of the tank 15, into the collection 17 whose slope facilitates the evacuation of the washing fluid by the evacuation pipe 18 connected to the collection 17.

A noter que dans les modes de realisation representes aux figures 1 à 4, qui ne comportent pas d'alimentation et d'évacuation en continu du fluide de lavage, les cuves 2 peuvent avantageusement être alimentes en fluide de lavage à l'aide d'une batterie de pipettes montes en série connue sous le nom de "FINN PIPET", l'évacuation étant alors reali- see manuellement. Note that in the embodiments shown in Figures 1 to 4, which do not include continuous supply and discharge of the washing fluid, the tanks 2 can advantageously be supplied with washing fluid using a battery of pipettes connected in series known as "FINN PIPET", the evacuation then being carried out manually.

La plaque 8 à cuves multiples, comporte sur le pourtour de sa base, de même que la plaque 2 des figures 3 et 4, un rebord 19 qui permet son empilement sur d'autres plaques identiques à la plaque 8 ou à la plaque 2. The plate 8 with multiple tanks, comprises on the periphery of its base, like the plate 2 of FIGS. 3 and 4, a flange 19 which allows it to be stacked on other plates identical to the plate 8 or to the plate 2.

Les plaques à cuves multiples conformes à l'invention sont avantageusement realisees en matière plastique rigide ou semi-rigide, telle, notamment que poly(chlorure de vinyle) ou autre matière plastique similaire, par moulage, ce qui permet de les fabriquer à l'echelle industrielle à bas prix. The multi-tank plates according to the invention are advantageously made of rigid or semi-rigid plastic material, such as in particular poly (vinyl chloride) or other similar plastic material, by molding, which makes it possible to manufacture them by industrial scale at low prices.

Ainsi que cela ressort de ce qui precède, l'invention ne se limite nullement a' ceux de ses modes de realisa- tion et d'application qui viennent d'être decrits de façon plus explicite ; elle en embrasse au contraire toutes les variantes qui peuvent venir à l'esprit du technicien en la matière, sans s'ecarter du cadre, ni de la portée, de la presente invention.  As is apparent from the above, the invention is in no way limited to those of its embodiments and of application which have just been described more explicitly; on the contrary, it embraces all the variants which may come to the mind of the technician in the matter, without departing from the framework, or the scope, of the present invention.

Claims (3)

REVENDICATIONS 1") Plaque à cuves multiples isolees les unes des autres, à usages biologiques, caracterisee en ce qu'elle comprend une pluralitg de cuves (2,15) de forme oblongue parallèles entre elles, dont la longueur est sensiblement egale à l'une des dimensions (longueur ou largeur) de la plaque (1,8) et en ce que lesdites cuves (2,15) sont separees les unes des autres par des parois (6,20) venues de fabrication, dont l'epaisseur est suffisante pour assurer la planeite et eventuellement la rigidite de la plaque. 2") Plaque à cuves multiples selon la revendication 1, caracterisee en ce que le fond (5) des cuves (2,15) est plat. 3e) Plaque à cuves multiples selon la revendication 1 ou la revendication 2, caracterisee en ce qu'elle comporte entre les parois d'extremite des cuves (2) et l'arrête correspondante de la plaque (1! une plage plane (3) propre à recevoir des indications d'identification de chacune des cuves (2). 4 ) Plaque à cuves multiples selon la revendication 3, caracterisee en ce que ladite plage plane (3) est delimitee entre une seule des extremites des cuves (2) et l'arrête (4) correspondante de la plaque (1). 5 ) Plaque à cuves multiples selon l'une quelconque des revendications 1 à 4, caractérisee en ce que le rapport hauteur/largeur de chaque cuve (2,15) est compris entre 2/1 et 2,5/1. 6 ) Plaque à cuves multiples selon l'une quelconque des revendications 1 à 5, caractérisée en ce que le rapport longueur/hauteur/largeur de chaque cuve (2,15) est compris entre 16/2/1 et 18/2,5/1. 7 ) Plaque à cuves multiples selon l'une quelconque des revendications 1 à 6, caracterisee en ce que le rapport largeur de cuve/epaisseur de paroi longitudinale de chaque cuve (2,15) est de l'ordre de 2,5/1 à 3,5/1. 8 ) Plaque à cuves multiples selon l'une quelconque des revendications 1 à 7, caracterisee en ce qu'elle est pourvue sur le pourtour de sa base d'un rebord (7,19) propre à permettre son empilement sur une autre plaque identique. 9 ) Plaque à cuves multiples selon l'une quelconque des revendications 1 à 8, caractérisée en ce qu'elle est pourvue de moyens de raccordement avec un tuyau d'alimentation en fluide de lavage et de moyens d'evacuation du fluide de lavage. 10 ) Plaque à cuves multiples selon la revendication 9, caracterisee en ce que lesdits moyens de raccordement comprennent un orifice tube (9) menage en partie haute de la plaque (8), connecte à un tuyau (10) d'alimentation en fluide de lavage. 11") Plaque à cuves multiples selon la revendication 9, caracterisee en ce que lesdits moyens d'evacuation comprennent en partie basse de la plaque (8) une collecte (17) en pente à I'extremité inferieure de laquelle est branche un tuyau (18) dlevacuation du fluide de lavage. 12") Plaque à cuves multiples selon l'une quelconque des revendications 9 à 11, caracterisee en ce qu'elle comporte du côte de la plaque (8) adjacent aux moyens de raccordement avec un tuyau (10) d'alimentation en fluide de lavage, une zone reservoir (11) à partir de laquelle le fluide de lavage se deverse dans les cuves (15). 13 ) Plaque à cuves multiples selon la revendication 12, caracterisee en ce que la zone reservoir (11) est delimi- tee exterieurement par la paroi laterale externe (12) correspondante de la plaque (8), et interieurement par la paroi d'extremité 113) correspondante de chaque cuve, dont la hauteur est legèrement inférieure à la hauteur de la paroi late- rale externe (12) de la plaque (8) afin de creer un tropplein en direction des cuves (15), le fond du réservoir (11) tant forme par une entretoise (14) qui relie les deux parois (12,13) et qui est située à une hauteur sensiblement comprise entre le quart et le tiers de la hauteur delta paroi d'extre- mite (13) des cuves.CLAIMS 1 ") Plate with multiple tanks isolated from each other, for biological use, characterized in that it comprises a plurality of tanks (2,15) of oblong shape parallel to each other, the length of which is substantially equal to the one of the dimensions (length or width) of the plate (1.8) and in that said tanks (2.15) are separated from each other by walls (6.20) coming from manufacture, the thickness of which is sufficient to ensure the flatness and possibly the rigidity of the plate. 2 ") Plate with multiple tanks according to claim 1, characterized in that the bottom (5) of the tanks (2,15) is flat. 3e) multi-tank plate according to claim 1 or claim 2, characterized in that it comprises between the end walls of the tanks (2) and the corresponding stop of the plate (1! A flat area (3) suitable for receiving identification indications from each of the tanks (2) 4) Multi-tank plate according to claim 3, characterized in that said flat area (3) is delimited between only one of the ends of the tanks (2) and the corresponding stop (4) of the plate (1). 5) Plate with multiple tanks according to any one of claims 1 to 4, characterized in that the height / width ratio of each tank (2.15) is between 2/1 and 2.5 / 1. 6) Plate with multiple tanks according to any one of claims 1 to 5, characterized in that the length / height / width ratio of each tank (2.15) is between 16/2/1 and 18 / 2,5 / 1. 7) Plate with multiple tanks according to any one of claims 1 to 6, characterized in that the ratio tank width / longitudinal wall thickness of each tank (2.15) is of the order of 2.5 / 1 at 3.5 / 1. 8) Plate with multiple tanks according to any one of claims 1 to 7, characterized in that it is provided on the periphery of its base with a flange (7,19) suitable for allowing its stacking on another identical plate . 9) Plate with multiple tanks according to any one of claims 1 to 8, characterized in that it is provided with connection means with a hose for supplying washing fluid and means for discharging the washing fluid. 10) multiple-tank plate according to claim 9, characterized in that said connection means comprise a tube orifice (9) leading in the upper part of the plate (8), connected to a pipe (10) for supplying fluid to washing. 11 ") Plate with multiple tanks according to claim 9, characterized in that said evacuation means comprise in the lower part of the plate (8) a collection (17) sloping at the lower end of which is connected a pipe ( 18) discharge of the washing fluid. 12 ") Multi-tank plate according to any one of claims 9 to 11, characterized in that it comprises on the side of the plate (8) adjacent to the connection means with a pipe ( 10) washing fluid supply, a reservoir area (11) from which the washing fluid spills into the tanks (15). 13) Multi-tank plate according to claim 12, characterized in that the reservoir zone (11) is delimited externally by the corresponding external lateral wall (12) of the plate (8), and internally by the end wall 113) corresponding to each tank, the height of which is slightly less than the height of the external lateral wall (12) of the plate (8) in order to create an overflow in the direction of the tanks (15), the bottom of the tank ( 11) both formed by a spacer (14) which connects the two walls (12,13) and which is situated at a height substantially between a quarter and a third of the height delta end wall (13) of the tanks . 140) Plaque à cuves multiples selon la revendication 13, caractérisée en ce que les entretoises susdites (14) reçoivent des indications d'identification de chacune des cuves. 140) Plate with multiple tanks according to claim 13, characterized in that the aforesaid spacers (14) receive identification indications from each of the tanks. 150) Plaque à cuves multiples selon la revendication 1, caractérisée en ce qu'elle comporte sur le pourtour de sa partie supérieure, un décrochement pour la mise en place d'un couvercle de fermeture. 150) Plate with multiple tanks according to claim 1, characterized in that it comprises on the periphery of its upper part, a recess for the establishment of a closure cover. 160) Application de la plaque à cuves multiples selon l'une quelconque des Revendications 1 à 15, à l'incubation et au lavage de bandelettes de papier ou de tout autre support, provenant d'une opération de séparation physico-chimique telle que électrophorese ou chromato- graphie.  160) Application of the multi-cell plate according to any one of Claims 1 to 15, to the incubation and washing of strips of paper or any other support, originating from a physicochemical separation operation such as electrophoresis or chromatography.
FR8311151A 1983-07-05 1983-07-05 NOVEL MULTIPLE CELL PLATE, PARTICULARLY FOR IMMUNOLOGICAL AND BIOLOGICAL REACTIONS Expired FR2548685B1 (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2652286A1 (en) * 1989-09-25 1991-03-29 Labsystems Oy Device and method for cleaning rods attached in rows to a plate in a laboratory apparatus
US6051423A (en) * 1996-03-13 2000-04-18 University Technologies International Inc. Biofilm assay
WO2002102963A3 (en) * 2001-06-14 2003-04-17 Millipore Corp Feeding tray for multiwell test apparatus
US6596505B2 (en) 2000-04-17 2003-07-22 University Technologies International, Inc. Apparatus and methods for testing effects of materials and surface coatings on the formation of biofilms
US6599696B2 (en) 2000-04-17 2003-07-29 University Technologies International, Inc. Effects of materials and surface coatings on encrustation and biofilm formation
US7166257B2 (en) * 2001-06-14 2007-01-23 Millipore Corporation Multiwell test apparatus

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA972302A (en) * 1973-04-12 1975-08-05 Daniel H. Atwood Core tray

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA972302A (en) * 1973-04-12 1975-08-05 Daniel H. Atwood Core tray

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2652286A1 (en) * 1989-09-25 1991-03-29 Labsystems Oy Device and method for cleaning rods attached in rows to a plate in a laboratory apparatus
US6051423A (en) * 1996-03-13 2000-04-18 University Technologies International Inc. Biofilm assay
US6326190B1 (en) * 1996-03-13 2001-12-04 University Technologies International, Inc. Biofilm assay
US7041470B2 (en) 1996-03-13 2006-05-09 University Technologies International, Inc. Method of assaying the susceptibility of biofilms to anti-microbial agents
US6596505B2 (en) 2000-04-17 2003-07-22 University Technologies International, Inc. Apparatus and methods for testing effects of materials and surface coatings on the formation of biofilms
US6599696B2 (en) 2000-04-17 2003-07-29 University Technologies International, Inc. Effects of materials and surface coatings on encrustation and biofilm formation
WO2002102963A3 (en) * 2001-06-14 2003-04-17 Millipore Corp Feeding tray for multiwell test apparatus
US7148060B2 (en) 2001-06-14 2006-12-12 Millipore Corporation Feeding tray for multiwell test apparatus
US7166257B2 (en) * 2001-06-14 2007-01-23 Millipore Corporation Multiwell test apparatus

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