FR2450262A1 - Plasmid(s) contg. DNA fragment attached to operon - for transformation into host bacterium on yeast to produce nutrient proteins, esp. ovalbumin from E. coli (NL 11.12.79) albumin - Google Patents

Plasmid(s) contg. DNA fragment attached to operon - for transformation into host bacterium on yeast to produce nutrient proteins, esp. ovalbumin from E. coli (NL 11.12.79) albumin

Info

Publication number
FR2450262A1
FR2450262A1 FR7905202A FR7905202A FR2450262A1 FR 2450262 A1 FR2450262 A1 FR 2450262A1 FR 7905202 A FR7905202 A FR 7905202A FR 7905202 A FR7905202 A FR 7905202A FR 2450262 A1 FR2450262 A1 FR 2450262A1
Authority
FR
France
Prior art keywords
plasmid
operon
esp
dna fragment
coli
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7905202A
Other languages
French (fr)
Other versions
FR2450262B1 (en
Inventor
Philippe Kourilsky
Odile Puijalon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Pasteur de Lille
Original Assignee
Institut Pasteur de Lille
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Pasteur de Lille filed Critical Institut Pasteur de Lille
Priority to FR7905202A priority Critical patent/FR2450262A1/en
Publication of FR2450262A1 publication Critical patent/FR2450262A1/en
Application granted granted Critical
Publication of FR2450262B1 publication Critical patent/FR2450262B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/77—Ovalbumin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/008—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from microorganisms

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Polymers & Plastics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Novel plasmid contains, in a part non-essential to its life cycle, a DNA fragment derived from a lambda -phage. This DNA fragment (insertion) is attached to one part of a bacterial operon (esp. a lactose operon) which contains at least the bacterial promoter and, attached to this promoter, a structure gene fragment (e.g. the Z-gene which codes for the formation of beta-galactosidase in the lactose operon). The insertion is opt. restricted to the first nucleotide hiplet(s) which code for the first amino acid(s) of the corresp. protein i.e. beta-galactosidase in the case of the lactose operon. The plasmid also contains, in the structure gene fragment, a point of intersection for a specific endonuclease such as EcoRI, and pref. no more characteristic sequences for the same endonuclease in any other part. The DNA fragments, after insertion in a suitable vector (plasmid) and transformation in a host cell lead to the formation of prokaryotic and eukaryotic proteins. This is used to produce nutrient proteins from a bacterium or yeast and is esp. suitable for the prodn. of oralbumin from E. coli. The process is not possible with natural genes. The DNA fragments are considerably larger than the previously produced synthetic somatostatin genes. The process is economical and the greater part of the protein formed is composed of th desired protein fragments.
FR7905202A 1979-02-28 1979-02-28 Plasmid(s) contg. DNA fragment attached to operon - for transformation into host bacterium on yeast to produce nutrient proteins, esp. ovalbumin from E. coli (NL 11.12.79) albumin Granted FR2450262A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7905202A FR2450262A1 (en) 1979-02-28 1979-02-28 Plasmid(s) contg. DNA fragment attached to operon - for transformation into host bacterium on yeast to produce nutrient proteins, esp. ovalbumin from E. coli (NL 11.12.79) albumin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7905202A FR2450262A1 (en) 1979-02-28 1979-02-28 Plasmid(s) contg. DNA fragment attached to operon - for transformation into host bacterium on yeast to produce nutrient proteins, esp. ovalbumin from E. coli (NL 11.12.79) albumin

Publications (2)

Publication Number Publication Date
FR2450262A1 true FR2450262A1 (en) 1980-09-26
FR2450262B1 FR2450262B1 (en) 1982-11-05

Family

ID=9222577

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7905202A Granted FR2450262A1 (en) 1979-02-28 1979-02-28 Plasmid(s) contg. DNA fragment attached to operon - for transformation into host bacterium on yeast to produce nutrient proteins, esp. ovalbumin from E. coli (NL 11.12.79) albumin

Country Status (1)

Country Link
FR (1) FR2450262A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2511033A1 (en) * 1981-08-10 1983-02-11 Genex Corp EXPRESSION VECTORS FOR INTRODUCING A GENE INTO A PROCARYOTE ORGANISM

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2176937A1 (en) * 1972-03-21 1973-11-02 Nestle Sa
FR2180752A1 (en) * 1972-04-18 1973-11-30 Zyma Sa Edible protein compsns - with reduced odour and taste prepd from living unicellular organisms esp yeasts
DE2433930A1 (en) * 1974-07-15 1976-02-05 Unisearch Ltd Enzymatic control of cpds. in soln - in a dual filter appts. with intermediate enzyme treatment stage

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2176937A1 (en) * 1972-03-21 1973-11-02 Nestle Sa
FR2180752A1 (en) * 1972-04-18 1973-11-30 Zyma Sa Edible protein compsns - with reduced odour and taste prepd from living unicellular organisms esp yeasts
DE2433930A1 (en) * 1974-07-15 1976-02-05 Unisearch Ltd Enzymatic control of cpds. in soln - in a dual filter appts. with intermediate enzyme treatment stage

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CA1977 *
CA1978 *
EXBK/77 *
EXBK/78 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2511033A1 (en) * 1981-08-10 1983-02-11 Genex Corp EXPRESSION VECTORS FOR INTRODUCING A GENE INTO A PROCARYOTE ORGANISM

Also Published As

Publication number Publication date
FR2450262B1 (en) 1982-11-05

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