RU94032791A - Dna fragments, recombinant plasmid dna and a method of nitrile hydratase preparing
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Dna fragments, recombinant plasmid dna and a method of nitrile hydratase preparing
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Publication number
RU94032791A
RU94032791ARU94032791/13ARU94032791ARU94032791ARU 94032791 ARU94032791 ARU 94032791ARU 94032791/13 ARU94032791/13 ARU 94032791/13ARU 94032791 ARU94032791 ARU 94032791ARU 94032791 ARU94032791 ARU 94032791A
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FIELD: molecular biology, genetic engineering, enzymes. SUBSTANCE: invention relates to the amino acid sequence and nucleotide sequence of α- and b-subunits of nitrile hydratase from Rhodococcus rhodochrous J-1. DNA fragment encoding nitrile hydratase is inserted in expression vector and recombinant vector is used for transformation. Transformant has multiple copies of gene and can produce nitrile hydratase at the enhanced expression level as compared with the usual microorganisms used. EFFECT: enhanced expression of enzyme.
Claims (1)
Предложена аминокислотная последовательность и нуклеотидная последовательность α- и b-субъединиц нитрил-гидратазы, происходящей из Rhodococcus rhodochrous J-1. Фрагмент ДНК, кодирующий нитрил-гидратазу, внедряют в вектор экспрессии и рекомбинантный вектор применяют для трансформации. Трансформант содержит множество копий гена и способен продуцировать нитрил-гидратазу на гораздо более высоком уровне по сравнению с обычно применяемыми микроорганизмами.The amino acid sequence and nucleotide sequence of the α- and b-subunits of nitrile hydratase derived from Rhodococcus rhodochrous J-1 are proposed. A DNA fragment encoding a nitrile hydratase is introduced into the expression vector and the recombinant vector is used for transformation. The transformant contains many copies of the gene and is capable of producing nitrile hydratase at a much higher level compared to commonly used microorganisms.
RU94032791/13A1990-02-281994-09-07Dna fragments, recombinant plasmid dna and a method of nitrile hydratase preparing
RU94032791A
(en)
Isolated nucleic acid sequence, nucleic acid construct, vector, recombinant microorganism, and process for the preparation of chiral or aquiral carboxylic acids
A gene encoding a polypeptide having nitrile hydratase activity, a transformant containing the gene and a process for the production of amides using the transformant