FR2443679A1 - Procede pour la determination de la concentration bacterienne par luminescence - Google Patents

Procede pour la determination de la concentration bacterienne par luminescence

Info

Publication number
FR2443679A1
FR2443679A1 FR7834680A FR7834680A FR2443679A1 FR 2443679 A1 FR2443679 A1 FR 2443679A1 FR 7834680 A FR7834680 A FR 7834680A FR 7834680 A FR7834680 A FR 7834680A FR 2443679 A1 FR2443679 A1 FR 2443679A1
Authority
FR
France
Prior art keywords
microbial
reactive
extracted
luminescence
type cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
FR7834680A
Other languages
English (en)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Vitatect Corp
Original Assignee
Vitatect Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vitatect Corp filed Critical Vitatect Corp
Priority to FR7834680A priority Critical patent/FR2443679A1/fr
Publication of FR2443679A1 publication Critical patent/FR2443679A1/fr
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G01N21/763—Bioluminescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2304/00—Chemical means of detecting microorganisms
    • C12Q2304/60—Chemiluminescent detection using ATP-luciferin-luciferase system

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plasma & Fusion (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procédé pour déterminer des faibles teneurs de cellules microbiennes dans des échantillons de fluides et dans des solides désagrégés. Selon l'invention, on traite l'échantillon de façon à broyer tous les solides présents dans l'échantillon, on fait éclater les parois de toutes les cellules non microbiennes pour extraire la matière microbienne qui s'y trouve, on filtre l'échantillon pour en éliminer toute la matière extraite, on fait éclater les parois des cellules microbiennes pour libérer la matière réactive qui s'y trouve, on introduit un réactif provoquant une réaction de luminescence pour saturer la matière réactive microbienne extraite, de préférence tandis que le résidu se trouve sur la surface du filtre et on décèle et enregistre une indication de l'intensité de luminescence émise par la réaction entre l'agent réactif et la matière réactive microbienne.
FR7834680A 1978-12-08 1978-12-08 Procede pour la determination de la concentration bacterienne par luminescence Pending FR2443679A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7834680A FR2443679A1 (fr) 1978-12-08 1978-12-08 Procede pour la determination de la concentration bacterienne par luminescence

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7834680A FR2443679A1 (fr) 1978-12-08 1978-12-08 Procede pour la determination de la concentration bacterienne par luminescence

Publications (1)

Publication Number Publication Date
FR2443679A1 true FR2443679A1 (fr) 1980-07-04

Family

ID=9215869

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7834680A Pending FR2443679A1 (fr) 1978-12-08 1978-12-08 Procede pour la determination de la concentration bacterienne par luminescence

Country Status (1)

Country Link
FR (1) FR2443679A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0105413A2 (fr) * 1982-09-30 1984-04-18 Miles Laboratories, Inc. Détection de cellules par dosage de la dinucléotide adénine flavine
EP0113103A1 (fr) * 1982-12-27 1984-07-11 EASTMAN KODAK COMPANY (a New Jersey corporation) Inhibition des activités réductrices de leucocytes
EP0126019A2 (fr) * 1983-05-16 1984-11-21 Packard Instrument Company, Inc. Méthode à haute résolution pour la mesure de l'ATP et pour la concentration et mesure d'organismes unicellulaires
EP0566010A1 (fr) * 1992-04-13 1993-10-20 BIOLAC GmbH Gesellschaft für industrielle Nutzung von Milchinhaltsstoffen Procédé et kit d'analyse pour déterminer le nombre de germes bactériens dans le petit-lait et ses composants

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0105413A2 (fr) * 1982-09-30 1984-04-18 Miles Laboratories, Inc. Détection de cellules par dosage de la dinucléotide adénine flavine
EP0105413A3 (fr) * 1982-09-30 1984-12-05 Miles Laboratories, Inc. Détection de cellules par dosage de la dinucléotide adénine flavine
EP0113103A1 (fr) * 1982-12-27 1984-07-11 EASTMAN KODAK COMPANY (a New Jersey corporation) Inhibition des activités réductrices de leucocytes
EP0126019A2 (fr) * 1983-05-16 1984-11-21 Packard Instrument Company, Inc. Méthode à haute résolution pour la mesure de l'ATP et pour la concentration et mesure d'organismes unicellulaires
EP0126019A3 (fr) * 1983-05-16 1986-06-11 Packard Instrument Company, Inc. Méthode à haute résolution pour la mesure de l'ATP et pour la concentration et mesure d'organismes unicellulaires
EP0566010A1 (fr) * 1992-04-13 1993-10-20 BIOLAC GmbH Gesellschaft für industrielle Nutzung von Milchinhaltsstoffen Procédé et kit d'analyse pour déterminer le nombre de germes bactériens dans le petit-lait et ses composants

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