FR2433751A1 - Methode pour determiner une transaminase dans un fluide biologique et combinaison de reactifs a utiliser dans la methode - Google Patents
Methode pour determiner une transaminase dans un fluide biologique et combinaison de reactifs a utiliser dans la methodeInfo
- Publication number
- FR2433751A1 FR2433751A1 FR7920611A FR7920611A FR2433751A1 FR 2433751 A1 FR2433751 A1 FR 2433751A1 FR 7920611 A FR7920611 A FR 7920611A FR 7920611 A FR7920611 A FR 7920611A FR 2433751 A1 FR2433751 A1 FR 2433751A1
- Authority
- FR
- France
- Prior art keywords
- glutamate
- transaminase
- fluid
- adenine dinucleotide
- beta
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
La présente invention concerne une méthode pour déterminer le glutamate oxalacétate transaminase ou glutamate pyruvate transaminase dans un fluide biologique, où du L-glutamate est produit à partir d'alpha-cétoglutarate par transamination en présence de la transaminase dans le fluide, et le L-glutamate est désaminé par oxydation en présence de glutamate déhydrogénase avec production simultanée de béta-nicotinamide adénine dinucléotide réduit en une quantité proportionnelle à la teneur de la transaminase dans le fluide, ce fluide contenant également une substance endogène oxydée en présence de glutamate déhydrogénase avec production simultanée de béta-nicotinamide adénine dinucléotide réduit, interférant ainsi avec la détermination. Selon l'invention, on obvie à l'interférence provoquée par la substance endogène en oxydant cette substance en présence de glutamate déhydrogénase avec réduction simultanée de béta-nicotinamide adénine dinucléotide, on oxyde le béta-nicotinamide adénine dinucléotide réduit résultant en présence de lactate déhydrogénase avec réduction simultanée de pyruvate en lactate, et on inhibe ensuite l'activité de lactate déhydrogénase, ensuite on produit une certaine quantité de L-glutamate à partir de l'apha-cétoglutarate par la transamination pour la désamination par oxydation et production simultanée de bétanicotinamide adénine dinucléotide réduit en une quantité proportionnelle à la teneur de la transaminase dans le fluide. L'invention s'applique notamment à l'analyse du sérum du sang.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US05/933,183 US4241179A (en) | 1978-08-14 | 1978-08-14 | Method for determining a transaminase in a biological fluid and reagent combination for use in the method |
Publications (1)
Publication Number | Publication Date |
---|---|
FR2433751A1 true FR2433751A1 (fr) | 1980-03-14 |
Family
ID=25463521
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7920611A Pending FR2433751A1 (fr) | 1978-08-14 | 1979-08-13 | Methode pour determiner une transaminase dans un fluide biologique et combinaison de reactifs a utiliser dans la methode |
Country Status (7)
Country | Link |
---|---|
US (1) | US4241179A (fr) |
JP (1) | JPS5526899A (fr) |
BE (1) | BE878224A (fr) |
DE (1) | DE2932748A1 (fr) |
FR (1) | FR2433751A1 (fr) |
GB (1) | GB2028500A (fr) |
NL (1) | NL7906143A (fr) |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2913553C2 (de) * | 1979-04-04 | 1981-09-17 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und Reagenz zur enzymatischen Bestimmung von Enzymsubstraten |
IT1162349B (it) * | 1979-07-17 | 1987-03-25 | Biodata Spa | Metodo per la determinazione delle transaminasi e relativo kit diagnostico |
US4450232A (en) * | 1982-02-04 | 1984-05-22 | Eastman Kodak Company | Incorporation of pyridoxal phosphate in dry analytical elements for the determination of enzymes |
US4575488A (en) * | 1982-06-25 | 1986-03-11 | Technicon Instruments Corp. | Interference free transaminase assay |
JPS59183698A (ja) * | 1983-04-02 | 1984-10-18 | Wako Pure Chem Ind Ltd | 基質又は酵素活性の定量方法 |
JPS6394994A (ja) * | 1986-10-09 | 1988-04-26 | Fuji Photo Film Co Ltd | 酸化型補酵素含有乾式分析要素 |
CA2053868A1 (fr) * | 1990-03-01 | 1992-08-28 | Kenneth J. Gruys | Methode d'utilisation d'un sous-produit de reaction pour etalonner des dosages enzymatiques |
US5834226A (en) * | 1991-01-31 | 1998-11-10 | Xytronyx, Inc. | One-step test for aspartate aminotransferase |
US5366870A (en) * | 1993-04-02 | 1994-11-22 | Xytronyx, Inc. | Limited enzyme assay for aminotransferases |
US5705045A (en) * | 1995-08-29 | 1998-01-06 | Lg Electronics Inc. | Multi-biosensor for GPT and got activity |
JP3756832B2 (ja) * | 2002-03-12 | 2006-03-15 | 株式会社三菱化学ヤトロン | アラニンアミノトランスフェラーゼ活性測定試薬 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4024021A (en) * | 1973-07-19 | 1977-05-17 | The Dow Chemical Company | Determination of glutamate and glutamic transaminases |
AT339505B (de) * | 1974-03-14 | 1977-10-25 | Boehringer Mannheim Gmbh | Enzymatisches analysenverfahren |
US3956069A (en) * | 1974-04-29 | 1976-05-11 | Abbott Laboratories | Enzymatic assays for glucose, creatine phosphokinase or plasma ammonia |
JPS5441520B2 (fr) * | 1974-10-23 | 1979-12-08 | ||
US4086142A (en) * | 1976-12-06 | 1978-04-25 | The Dow Chemical Company | Decarboxylation of endogenous serum glutamate in transaminase assays |
-
1978
- 1978-08-14 US US05/933,183 patent/US4241179A/en not_active Expired - Lifetime
-
1979
- 1979-08-13 NL NL7906143A patent/NL7906143A/nl not_active Application Discontinuation
- 1979-08-13 BE BE0/196717A patent/BE878224A/fr unknown
- 1979-08-13 FR FR7920611A patent/FR2433751A1/fr active Pending
- 1979-08-13 GB GB7928173A patent/GB2028500A/en not_active Withdrawn
- 1979-08-13 DE DE19792932748 patent/DE2932748A1/de not_active Withdrawn
- 1979-08-13 JP JP10307379A patent/JPS5526899A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
US4241179A (en) | 1980-12-23 |
GB2028500A (en) | 1980-03-05 |
NL7906143A (nl) | 1980-02-18 |
BE878224A (fr) | 1980-02-13 |
DE2932748A1 (de) | 1980-03-06 |
JPS5526899A (en) | 1980-02-26 |
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