FR2414729A1 - Determination of creatine kinase isoenzyme(s) - using immobilised isoenzyme and a marked specific antibody associated with an enzyme - Google Patents

Determination of creatine kinase isoenzyme(s) - using immobilised isoenzyme and a marked specific antibody associated with an enzyme

Info

Publication number
FR2414729A1
FR2414729A1 FR7900344A FR7900344A FR2414729A1 FR 2414729 A1 FR2414729 A1 FR 2414729A1 FR 7900344 A FR7900344 A FR 7900344A FR 7900344 A FR7900344 A FR 7900344A FR 2414729 A1 FR2414729 A1 FR 2414729A1
Authority
FR
France
Prior art keywords
isoenzyme
enzyme
creatine kinase
immobilised
specific antibody
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
FR7900344A
Other languages
French (fr)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SmithKline Instrument Inc
Original Assignee
SmithKline Instrument Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SmithKline Instrument Inc filed Critical SmithKline Instrument Inc
Publication of FR2414729A1 publication Critical patent/FR2414729A1/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/70—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving creatine or creatinine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/007—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isoenzyme profiles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

Abstract

Creatine kinase isoenzymes are detected and determined in a liq. sample. A creatine kinase isoenzyme, immobilised on a water-insoluble support is incubated with a specific antibody for the isoenzyme sub-unit which is associated covalently with an enzyme, and also with the sample of liq. contg. the isoenzyme which is to be determined. The insoluble support is sepd., and the activity of the enzyme associated with the antibody fixed on the insoluble support is determined, giving a measure of the isoenzyme sub-unit present in the sample. Used for diagnosis of specific disorders, such as myocardial infarctus.
FR7900344A 1978-01-16 1979-01-08 Determination of creatine kinase isoenzyme(s) - using immobilised isoenzyme and a marked specific antibody associated with an enzyme Pending FR2414729A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US86973378A 1978-01-16 1978-01-16

Publications (1)

Publication Number Publication Date
FR2414729A1 true FR2414729A1 (en) 1979-08-10

Family

ID=25354161

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7900344A Pending FR2414729A1 (en) 1978-01-16 1979-01-08 Determination of creatine kinase isoenzyme(s) - using immobilised isoenzyme and a marked specific antibody associated with an enzyme

Country Status (6)

Country Link
JP (1) JPS54119291A (en)
BE (1) BE873465A (en)
DE (1) DE2901391A1 (en)
DK (1) DK10579A (en)
FR (1) FR2414729A1 (en)
SE (1) SE7900335L (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0467782A1 (en) * 1990-07-18 1992-01-22 International Immunoassay Laboratories, Inc. Myocardial infarction immunoassay

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108548926B (en) * 2018-03-22 2019-11-08 北京九强生物技术股份有限公司 A kind of creatine kinase isozyme detection kit
CN116731185B (en) * 2023-06-27 2023-11-10 安徽千诚生物技术有限公司 Creatine kinase isozyme antibody and mitochondria CK detection kit

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3654090A (en) * 1968-09-24 1972-04-04 Organon Method for the determination of antigens and antibodies
FR2192144A1 (en) * 1972-07-10 1974-02-08 Miles Lab Specific isoenzyme antibodies polymn - for determination of isoenzyme in liq specimens
US3932221A (en) * 1971-06-09 1976-01-13 Merck Patent Gesellschaft Mit Beschrankter Haftung Immunological isozyme determination method
US4012285A (en) * 1972-12-01 1977-03-15 Merck Patent Gesellschaft Mit Beschrankter Haftung Analysis of isoenzyme patterns

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3654090A (en) * 1968-09-24 1972-04-04 Organon Method for the determination of antigens and antibodies
US3654090B1 (en) * 1968-09-24 1982-07-20
US3932221A (en) * 1971-06-09 1976-01-13 Merck Patent Gesellschaft Mit Beschrankter Haftung Immunological isozyme determination method
FR2192144A1 (en) * 1972-07-10 1974-02-08 Miles Lab Specific isoenzyme antibodies polymn - for determination of isoenzyme in liq specimens
US4012285A (en) * 1972-12-01 1977-03-15 Merck Patent Gesellschaft Mit Beschrankter Haftung Analysis of isoenzyme patterns

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CA1975 *
CA1977 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0467782A1 (en) * 1990-07-18 1992-01-22 International Immunoassay Laboratories, Inc. Myocardial infarction immunoassay

Also Published As

Publication number Publication date
DK10579A (en) 1979-07-17
BE873465A (en) 1979-07-16
DE2901391A1 (en) 1979-07-19
SE7900335L (en) 1979-09-20
JPS54119291A (en) 1979-09-17

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