FR2406199A1 - Procede pour determiner des iso-enzymes - Google Patents
Procede pour determiner des iso-enzymesInfo
- Publication number
- FR2406199A1 FR2406199A1 FR7826096A FR7826096A FR2406199A1 FR 2406199 A1 FR2406199 A1 FR 2406199A1 FR 7826096 A FR7826096 A FR 7826096A FR 7826096 A FR7826096 A FR 7826096A FR 2406199 A1 FR2406199 A1 FR 2406199A1
- Authority
- FR
- France
- Prior art keywords
- isoenzymes
- substrate
- enzymes
- isoenzyme
- determination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/007—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isoenzyme profiles
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
L'invention concerne la détermination d'iso-enzymes. Pour déterminer la présence d'iso-enzymes ayant des charges éléctriques globales différentes, on sépare par chromatographie les iso-enzymes sur une colonne échangeuse d'anions, on élue et on receuille séparément les fractions contenant chacun des iso-enzymes à déterminer en utilisant chaque fraction pour reconstituer un réactif sec de l'iso-enzyme en l'absence d'un substrat de l'iso-enzyme, on ajoute au réactif reconstitué un substrat de l'enzyme et on analyse par spectrophotométrie le réactif reconstitué additionné avec le substrat. L'invention s'applique en particulier à la détermination des iso-enzymes de la créatine-phosphokinase.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US84124877A | 1977-10-11 | 1977-10-11 |
Publications (1)
Publication Number | Publication Date |
---|---|
FR2406199A1 true FR2406199A1 (fr) | 1979-05-11 |
Family
ID=25284400
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7826096A Pending FR2406199A1 (fr) | 1977-10-11 | 1978-09-12 | Procede pour determiner des iso-enzymes |
Country Status (5)
Country | Link |
---|---|
JP (1) | JPS5465096A (fr) |
BE (1) | BE871117A (fr) |
DE (1) | DE2844313A1 (fr) |
FR (1) | FR2406199A1 (fr) |
GB (1) | GB2005830B (fr) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SE7905852L (sv) * | 1979-07-04 | 1981-01-05 | Lkb Produkter Ab | Forfarande for bestemning av kreatinkinas |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2214887A1 (en) * | 1973-01-19 | 1974-08-19 | Boehringer Mannheim Gmbh | Creatine kinase determination - using N-acetyl cystein as activator in reaction of creatine phosphate and ADP to form creatine and ATP |
FR2255601A1 (fr) * | 1973-12-19 | 1975-07-18 | Mercer Donald | |
US4013513A (en) * | 1976-01-30 | 1977-03-22 | E-C Apparatus Corporation | Ion exchange chromatographic isoenzyme separation and isolation |
US4105499A (en) * | 1976-10-06 | 1978-08-08 | Kiyasu John Y | Heart attack screening method, apparatus and kit for same |
-
1978
- 1978-08-13 GB GB7833780A patent/GB2005830B/en not_active Expired
- 1978-09-12 FR FR7826096A patent/FR2406199A1/fr active Pending
- 1978-10-09 BE BE6046632A patent/BE871117A/fr unknown
- 1978-10-09 JP JP12455478A patent/JPS5465096A/ja active Pending
- 1978-10-11 DE DE19782844313 patent/DE2844313A1/de not_active Withdrawn
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2214887A1 (en) * | 1973-01-19 | 1974-08-19 | Boehringer Mannheim Gmbh | Creatine kinase determination - using N-acetyl cystein as activator in reaction of creatine phosphate and ADP to form creatine and ATP |
FR2255601A1 (fr) * | 1973-12-19 | 1975-07-18 | Mercer Donald | |
US4013513A (en) * | 1976-01-30 | 1977-03-22 | E-C Apparatus Corporation | Ion exchange chromatographic isoenzyme separation and isolation |
US4105499A (en) * | 1976-10-06 | 1978-08-08 | Kiyasu John Y | Heart attack screening method, apparatus and kit for same |
Non-Patent Citations (3)
Title |
---|
CA1977 * |
EXBK/68 * |
EXBK/74 * |
Also Published As
Publication number | Publication date |
---|---|
GB2005830A (en) | 1979-04-25 |
DE2844313A1 (de) | 1979-07-26 |
JPS5465096A (en) | 1979-05-25 |
BE871117A (fr) | 1979-04-09 |
GB2005830B (en) | 1982-05-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
TP | Transmission of property |