GB2005830A - Isoenzyme determination - Google Patents

Isoenzyme determination

Info

Publication number
GB2005830A
GB2005830A GB7833780A GB7833780A GB2005830A GB 2005830 A GB2005830 A GB 2005830A GB 7833780 A GB7833780 A GB 7833780A GB 7833780 A GB7833780 A GB 7833780A GB 2005830 A GB2005830 A GB 2005830A
Authority
GB
United Kingdom
Prior art keywords
isoenzymes
substrate
isoenzyme
reagent
reconstituted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB7833780A
Other versions
GB2005830B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
American Hospital Supply Corp
Original Assignee
American Hospital Supply Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Hospital Supply Corp filed Critical American Hospital Supply Corp
Publication of GB2005830A publication Critical patent/GB2005830A/en
Application granted granted Critical
Publication of GB2005830B publication Critical patent/GB2005830B/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/007—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving isoenzyme profiles

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for determining the presence of isoenzymes of different net electrical charges comprising chromatographically separating the isoenzymes on an anion-exchange column, separately eluting and collecting fractions containing the isoenzymes to be determined, using each fraction to reconstitute a dry reagent for the isoenzyme in the absence of a substrate for the isoenzyme, adding to the reconstituted reagent a substrate for the enzyme, and analyzing the reconstituted reagent and substrate spectrophotometrically.
GB7833780A 1977-10-11 1978-08-13 Isoenzyme determination Expired GB2005830B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US84124877A 1977-10-11 1977-10-11

Publications (2)

Publication Number Publication Date
GB2005830A true GB2005830A (en) 1979-04-25
GB2005830B GB2005830B (en) 1982-05-26

Family

ID=25284400

Family Applications (1)

Application Number Title Priority Date Filing Date
GB7833780A Expired GB2005830B (en) 1977-10-11 1978-08-13 Isoenzyme determination

Country Status (5)

Country Link
JP (1) JPS5465096A (en)
BE (1) BE871117A (en)
DE (1) DE2844313A1 (en)
FR (1) FR2406199A1 (en)
GB (1) GB2005830B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0022432A1 (en) * 1979-07-04 1981-01-14 LKB-Produkter AB Method for determination of creatine kinase

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2302721C2 (en) * 1973-01-19 1975-01-23 Boehringer Mannheim Gmbh, 6800 Mannheim Method for the determination of creatine kinase
SE7408786L (en) * 1973-12-19 1975-06-23 Donald William Mercer
US4013513A (en) * 1976-01-30 1977-03-22 E-C Apparatus Corporation Ion exchange chromatographic isoenzyme separation and isolation
US4105499A (en) * 1976-10-06 1978-08-08 Kiyasu John Y Heart attack screening method, apparatus and kit for same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0022432A1 (en) * 1979-07-04 1981-01-14 LKB-Produkter AB Method for determination of creatine kinase

Also Published As

Publication number Publication date
GB2005830B (en) 1982-05-26
FR2406199A1 (en) 1979-05-11
DE2844313A1 (en) 1979-07-26
BE871117A (en) 1979-04-09
JPS5465096A (en) 1979-05-25

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Legal Events

Date Code Title Description
732 Registration of transactions, instruments or events in the register (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee