FR2214887A1 - Creatine kinase determination - using N-acetyl cystein as activator in reaction of creatine phosphate and ADP to form creatine and ATP - Google Patents

Creatine kinase determination - using N-acetyl cystein as activator in reaction of creatine phosphate and ADP to form creatine and ATP

Info

Publication number
FR2214887A1
FR2214887A1 FR7401262A FR7401262A FR2214887A1 FR 2214887 A1 FR2214887 A1 FR 2214887A1 FR 7401262 A FR7401262 A FR 7401262A FR 7401262 A FR7401262 A FR 7401262A FR 2214887 A1 FR2214887 A1 FR 2214887A1
Authority
FR
France
Prior art keywords
creatine
activator
adp
atp
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
FR7401262A
Other languages
French (fr)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of FR2214887A1 publication Critical patent/FR2214887A1/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/50—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving creatine phosphokinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

In determination of creatine kinase by reaction of creatine phosphate with ADP to form creatine and aTP, in the presence of an SH gp-contg. activator and measuring quantitative change of a reactant N-acetyl cystein is used as the activator. Stability of N-acetyl cystein allows prepn. of reagents for medical diagnosis based on detecting CK-activity changes in serum, e.g. determining severity of heart attacks.
FR7401262A 1973-01-19 1974-01-15 Creatine kinase determination - using N-acetyl cystein as activator in reaction of creatine phosphate and ADP to form creatine and ATP Withdrawn FR2214887A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2302721A DE2302721C2 (en) 1973-01-19 1973-01-19 Method for the determination of creatine kinase

Publications (1)

Publication Number Publication Date
FR2214887A1 true FR2214887A1 (en) 1974-08-19

Family

ID=5869472

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7401262A Withdrawn FR2214887A1 (en) 1973-01-19 1974-01-15 Creatine kinase determination - using N-acetyl cystein as activator in reaction of creatine phosphate and ADP to form creatine and ATP

Country Status (9)

Country Link
JP (1) JPS49106397A (en)
AR (1) AR197431A1 (en)
BE (1) BE809499A (en)
BR (1) BR7309969D0 (en)
DD (1) DD108377A5 (en)
DE (1) DE2302721C2 (en)
FR (1) FR2214887A1 (en)
HU (1) HU165888B (en)
NL (1) NL7314996A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2299644A1 (en) * 1975-01-28 1976-08-27 Akzo Nv EVIDENCE AND DEBT
FR2406199A1 (en) * 1977-10-11 1979-05-11 American Hospital Supply Corp METHOD FOR DETERMINING ISO-ENZYMES
EP0015437A1 (en) * 1979-03-02 1980-09-17 Roche Diagnostics GmbH Method and reagent for the determination of creatine kinase

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1102225A (en) * 1976-09-13 1981-06-02 Ivan E. Modrovich Stabilized liquid enzyme and coenzyme compositions and method of preparing same
JPS5982398A (en) * 1982-11-01 1984-05-12 Toyobo Co Ltd Method for stabilizing coenzyme
JPS59162898A (en) * 1983-03-09 1984-09-13 Hitachi Ltd Method and apparatus for determination of solute using immobilized enzyme

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2299644A1 (en) * 1975-01-28 1976-08-27 Akzo Nv EVIDENCE AND DEBT
FR2406199A1 (en) * 1977-10-11 1979-05-11 American Hospital Supply Corp METHOD FOR DETERMINING ISO-ENZYMES
EP0015437A1 (en) * 1979-03-02 1980-09-17 Roche Diagnostics GmbH Method and reagent for the determination of creatine kinase

Also Published As

Publication number Publication date
DE2302721C2 (en) 1975-01-23
JPS49106397A (en) 1974-10-08
AR197431A1 (en) 1974-04-05
BE809499A (en) 1974-07-08
HU165888B (en) 1974-12-28
DD108377A5 (en) 1974-09-12
BR7309969D0 (en) 1974-09-24
NL7314996A (en) 1974-07-23
DE2302721B1 (en) 1974-05-30

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Legal Events

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ST Notification of lapse