FR2394553A1 - Nouveau procede d'extraction et de purification de 3 et de 3 -hydroxysteroidedeshydrogenase, enzymes pures ainsi obtenues et applications de ces enzymes a divers dosages et syntheses. - Google Patents

Nouveau procede d'extraction et de purification de 3 et de 3 -hydroxysteroidedeshydrogenase, enzymes pures ainsi obtenues et applications de ces enzymes a divers dosages et syntheses.

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Publication number
FR2394553A1
FR2394553A1 FR7703583A FR7703583A FR2394553A1 FR 2394553 A1 FR2394553 A1 FR 2394553A1 FR 7703583 A FR7703583 A FR 7703583A FR 7703583 A FR7703583 A FR 7703583A FR 2394553 A1 FR2394553 A1 FR 2394553A1
Authority
FR
France
Prior art keywords
estrone
caproate
agarose
alpha
amino
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7703583A
Other languages
English (en)
Other versions
FR2394553B1 (fr
Inventor
Bernard Descompd
Andre Crastes De Paulet
Jean-Claude Nicolas
Beatrice Terouanne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut National de la Sante et de la Recherche Medicale INSERM
Original Assignee
Institut National de la Sante et de la Recherche Medicale INSERM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut National de la Sante et de la Recherche Medicale INSERM filed Critical Institut National de la Sante et de la Recherche Medicale INSERM
Priority to FR7703583A priority Critical patent/FR2394553A1/fr
Publication of FR2394553A1 publication Critical patent/FR2394553A1/fr
Application granted granted Critical
Publication of FR2394553B1 publication Critical patent/FR2394553B1/fr
Granted legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J9/00—Normal steroids containing carbon, hydrogen, halogen or oxygen substituted in position 17 beta by a chain of more than two carbon atoms, e.g. cholane, cholestane, coprostane
    • C07J9/005—Normal steroids containing carbon, hydrogen, halogen or oxygen substituted in position 17 beta by a chain of more than two carbon atoms, e.g. cholane, cholestane, coprostane containing a carboxylic function directly attached or attached by a chain containing only carbon atoms to the cyclopenta[a]hydrophenanthrene skeleton
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Procédé d'extraction et de purification des enzymes 3 alpha HSDH et 3 beta HSDH. L'on extrait des cultures de Pseudomonas Testosteroni ou d'Escherichia Coli, par un tampon de pH voisin de 7, le surnageant obtenu étant saturé par du sulfate d'ammonium et le précipité résultant étant soumis, après redissolution et dialyse, à une chromatographie sur colonne, puis à une chromatographie d'affinité, les deux enzymes adsorbées étant ensuite éluées et séparées par des solutions salines neutres de concentration molaire comprise entre 0,5 et 1,5 M, susceptibles d'augmenter les interactions hydrophobes, notamment par des solutions de sulfate d'ammonium à gradient décroissant. Application des enzymes 3 alpha HSDH et 3 beta HSDH pures obtenues; aux dosages des stéroïdes, et en particulier des acides biliaires, ainsi qu'aux dosages des haptènes et, en ce qui concerne l'enzyme 3 alpha HSDH, à la synthèse de l'acide chenode-soxycholique.
FR7703583A 1977-02-09 1977-02-09 Nouveau procede d'extraction et de purification de 3 et de 3 -hydroxysteroidedeshydrogenase, enzymes pures ainsi obtenues et applications de ces enzymes a divers dosages et syntheses. Granted FR2394553A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7703583A FR2394553A1 (fr) 1977-02-09 1977-02-09 Nouveau procede d'extraction et de purification de 3 et de 3 -hydroxysteroidedeshydrogenase, enzymes pures ainsi obtenues et applications de ces enzymes a divers dosages et syntheses.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7703583A FR2394553A1 (fr) 1977-02-09 1977-02-09 Nouveau procede d'extraction et de purification de 3 et de 3 -hydroxysteroidedeshydrogenase, enzymes pures ainsi obtenues et applications de ces enzymes a divers dosages et syntheses.

Publications (2)

Publication Number Publication Date
FR2394553A1 true FR2394553A1 (fr) 1979-01-12
FR2394553B1 FR2394553B1 (fr) 1983-03-11

Family

ID=9186496

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7703583A Granted FR2394553A1 (fr) 1977-02-09 1977-02-09 Nouveau procede d'extraction et de purification de 3 et de 3 -hydroxysteroidedeshydrogenase, enzymes pures ainsi obtenues et applications de ces enzymes a divers dosages et syntheses.

Country Status (1)

Country Link
FR (1) FR2394553A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2567906A1 (fr) * 1984-07-23 1986-01-24 Toyo Jozo Kk Procede de dosage enzymatique tres sensible

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CA1975 *
CA1976 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2567906A1 (fr) * 1984-07-23 1986-01-24 Toyo Jozo Kk Procede de dosage enzymatique tres sensible

Also Published As

Publication number Publication date
FR2394553B1 (fr) 1983-03-11

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