FR2280082A1 - Hydrazine-stabilized bilirubin colorimetric assay - by coupling of free or conjugated bilirubin with diazonium salts - Google Patents

Hydrazine-stabilized bilirubin colorimetric assay - by coupling of free or conjugated bilirubin with diazonium salts

Info

Publication number
FR2280082A1
FR2280082A1 FR7425842A FR7425842A FR2280082A1 FR 2280082 A1 FR2280082 A1 FR 2280082A1 FR 7425842 A FR7425842 A FR 7425842A FR 7425842 A FR7425842 A FR 7425842A FR 2280082 A1 FR2280082 A1 FR 2280082A1
Authority
FR
France
Prior art keywords
bilirubin
coupling
hydrazine
free
conjugated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7425842A
Other languages
French (fr)
Other versions
FR2280082B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
REVEILLEAU R
Original Assignee
REVEILLEAU R
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by REVEILLEAU R filed Critical REVEILLEAU R
Priority to FR7425842A priority Critical patent/FR2280082A1/en
Publication of FR2280082A1 publication Critical patent/FR2280082A1/en
Application granted granted Critical
Publication of FR2280082B1 publication Critical patent/FR2280082B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/728—Bilirubin; including biliverdin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Novel method of estimating bilirubin in biological fluids in which bilirubin is coupled with a diazo-cpd. and the coupled prodt. is assayed colorimetrically in an alkaline medium, hydrazine being added as stabiliser to the reaction medium after addn. of the diazo-cpd. at a concn. of >=400 times, and pref. 600 times that of the diazo cpd. The colour developed after coupling is stabilised and a rigid time limit in the assay of free or conjugated bilirubin is avoided, excess diazonium salt present after coupling of the conjugated bilirubin is inhibited, and no adverse colouration is introduced to confuse the assay.
FR7425842A 1974-07-25 1974-07-25 Hydrazine-stabilized bilirubin colorimetric assay - by coupling of free or conjugated bilirubin with diazonium salts Granted FR2280082A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7425842A FR2280082A1 (en) 1974-07-25 1974-07-25 Hydrazine-stabilized bilirubin colorimetric assay - by coupling of free or conjugated bilirubin with diazonium salts

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7425842A FR2280082A1 (en) 1974-07-25 1974-07-25 Hydrazine-stabilized bilirubin colorimetric assay - by coupling of free or conjugated bilirubin with diazonium salts

Publications (2)

Publication Number Publication Date
FR2280082A1 true FR2280082A1 (en) 1976-02-20
FR2280082B1 FR2280082B1 (en) 1977-10-14

Family

ID=9141693

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7425842A Granted FR2280082A1 (en) 1974-07-25 1974-07-25 Hydrazine-stabilized bilirubin colorimetric assay - by coupling of free or conjugated bilirubin with diazonium salts

Country Status (1)

Country Link
FR (1) FR2280082A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2426263A1 (en) * 1978-07-17 1979-12-14 Beckman Instruments Inc

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2043035A5 (en) * 1969-04-07 1971-02-12 American Monitor Corp PROCEDURE FOR THE TEST AND TEST OF BILIRUBIN
FR2159921A5 (en) * 1971-11-01 1973-06-22 Dow Chemical Co

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2043035A5 (en) * 1969-04-07 1971-02-12 American Monitor Corp PROCEDURE FOR THE TEST AND TEST OF BILIRUBIN
FR2159921A5 (en) * 1971-11-01 1973-06-22 Dow Chemical Co

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2426263A1 (en) * 1978-07-17 1979-12-14 Beckman Instruments Inc

Also Published As

Publication number Publication date
FR2280082B1 (en) 1977-10-14

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Legal Events

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