FR2163363A1 - L-asparaginase purification - by t-butanol precipitation from aqs solns - Google Patents
L-asparaginase purification - by t-butanol precipitation from aqs solnsInfo
- Publication number
- FR2163363A1 FR2163363A1 FR7145211A FR7145211A FR2163363A1 FR 2163363 A1 FR2163363 A1 FR 2163363A1 FR 7145211 A FR7145211 A FR 7145211A FR 7145211 A FR7145211 A FR 7145211A FR 2163363 A1 FR2163363 A1 FR 2163363A1
- Authority
- FR
- France
- Prior art keywords
- aqs
- solns
- butanol
- degrees
- precipitation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
- C12N9/82—Asparaginase (3.5.1.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Purificn. is by precipitation from aqs. solns. with t-butanol pref. at 20-25 degrees C. The prodt. gives rise to less side-effects when administered as an antitumour agent. The process is simpler than known purifications using heat treatment or primary alcohol treatment. A preferred ratio is 0.3 to 0.6 vols of t-butanol to each volume of aqs. soln contg. 5-30 mg of protein/ml at 21 degrees C plus-or-minus 1 degrees C. A slow precipitation of enzyme in crystalline form is achieved.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR7145211A FR2163363A1 (en) | 1971-12-16 | 1971-12-16 | L-asparaginase purification - by t-butanol precipitation from aqs solns |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR7145211A FR2163363A1 (en) | 1971-12-16 | 1971-12-16 | L-asparaginase purification - by t-butanol precipitation from aqs solns |
Publications (2)
Publication Number | Publication Date |
---|---|
FR2163363A1 true FR2163363A1 (en) | 1973-07-27 |
FR2163363B1 FR2163363B1 (en) | 1975-02-07 |
Family
ID=9087571
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FR7145211A Granted FR2163363A1 (en) | 1971-12-16 | 1971-12-16 | L-asparaginase purification - by t-butanol precipitation from aqs solns |
Country Status (1)
Country | Link |
---|---|
FR (1) | FR2163363A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1088887A1 (en) * | 1999-09-23 | 2001-04-04 | Dsm N.V. | Crosslinked enzyme aggregates |
-
1971
- 1971-12-16 FR FR7145211A patent/FR2163363A1/en active Granted
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1088887A1 (en) * | 1999-09-23 | 2001-04-04 | Dsm N.V. | Crosslinked enzyme aggregates |
Also Published As
Publication number | Publication date |
---|---|
FR2163363B1 (en) | 1975-02-07 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
ST | Notification of lapse |