FR2074169A5 - Proteolytic enzyme prodn - Google Patents

Proteolytic enzyme prodn

Info

Publication number
FR2074169A5
FR2074169A5 FR7046263A FR7046263A FR2074169A5 FR 2074169 A5 FR2074169 A5 FR 2074169A5 FR 7046263 A FR7046263 A FR 7046263A FR 7046263 A FR7046263 A FR 7046263A FR 2074169 A5 FR2074169 A5 FR 2074169A5
Authority
FR
France
Prior art keywords
degrees
proteolytic enzyme
prodn
spectrum
proteolytic activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
FR7046263A
Other languages
French (fr)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19691965281 external-priority patent/DE1965281C3/en
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Application granted granted Critical
Publication of FR2074169A5 publication Critical patent/FR2074169A5/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C14—SKINS; HIDES; PELTS; LEATHER
    • C14C—CHEMICAL TREATMENT OF HIDES, SKINS OR LEATHER, e.g. TANNING, IMPREGNATING, FINISHING; APPARATUS THEREFOR; COMPOSITIONS FOR TANNING
    • C14C1/00—Chemical treatment prior to tanning
    • C14C1/06—Facilitating unhairing, e.g. by painting, by liming
    • C14C1/065—Enzymatic unhairing
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

Proteolytic enzyme, obtained by culturing Tritirachium album LIMBER aerobically (submerged) on a carbohydrate/org. N/inorg. N medium at 15-32 degrees C, at pH 5-8 is exocellular and has an element analysis of 41.4% C., 7.1% H, 13.3% N and 1.5% S., a m.wt. 20,000-30, 0, and I-R spectrum with absorption bands at 3300, 297 , 1645; 1537; 1514, 1452, 1405, 1238, and 1060 cm-1; a U. V. spectrum with a maximum at 36200 cm-1 (e1%1cm = 8.75); an isoelectric point at about pH 9.5; proteolytic activity at between pH 3.5 and 12.5 stability in aqs. soln buffered at pH 8-11 and contg. 1-5 x 10-3 Mol/l. Ca2+ at 25 degrees C., of at least 30 days the optimum temp for the proteolytic activity at pH 8 is 65-70 degrees C.
FR7046263A 1969-12-29 1970-12-22 Proteolytic enzyme prodn Expired FR2074169A5 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19691965281 DE1965281C3 (en) 1969-12-29 Proteolytic enzyme and process for its production

Publications (1)

Publication Number Publication Date
FR2074169A5 true FR2074169A5 (en) 1971-10-01

Family

ID=5755209

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7046263A Expired FR2074169A5 (en) 1969-12-29 1970-12-22 Proteolytic enzyme prodn

Country Status (3)

Country Link
BE (1) BE760902A (en)
FR (1) FR2074169A5 (en)
NL (1) NL7015726A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0317307A2 (en) * 1987-11-18 1989-05-24 Unilever Plc Enzymatic liquid detergent composition

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0317307A2 (en) * 1987-11-18 1989-05-24 Unilever Plc Enzymatic liquid detergent composition
EP0317307A3 (en) * 1987-11-18 1990-10-17 Unilever Plc Enzymatic liquid detergent composition

Also Published As

Publication number Publication date
BE760902A (en) 1971-06-28
DE1965281B2 (en) 1977-07-07
NL7015726A (en) 1971-07-01
DE1965281A1 (en) 1971-07-01

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Legal Events

Date Code Title Description
ST Notification of lapse