FI96367B - Biospesifik bestämningsmetod - Google Patents
Biospesifik bestämningsmetod Download PDFInfo
- Publication number
- FI96367B FI96367B FI944865A FI944865A FI96367B FI 96367 B FI96367 B FI 96367B FI 944865 A FI944865 A FI 944865A FI 944865 A FI944865 A FI 944865A FI 96367 B FI96367 B FI 96367B
- Authority
- FI
- Finland
- Prior art keywords
- microparticles
- different
- compounds
- dyes
- biospecific
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 16
- 239000011859 microparticle Substances 0.000 claims abstract description 26
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract 3
- 150000001875 compounds Chemical class 0.000 claims description 36
- 239000000975 dye Substances 0.000 claims description 26
- 230000005284 excitation Effects 0.000 claims description 18
- 239000007850 fluorescent dye Substances 0.000 claims description 13
- 239000002245 particle Substances 0.000 claims description 7
- OJURWUUOVGOHJZ-UHFFFAOYSA-N methyl 2-[(2-acetyloxyphenyl)methyl-[2-[(2-acetyloxyphenyl)methyl-(2-methoxy-2-oxoethyl)amino]ethyl]amino]acetate Chemical compound C=1C=CC=C(OC(C)=O)C=1CN(CC(=O)OC)CCN(CC(=O)OC)CC1=CC=CC=C1OC(C)=O OJURWUUOVGOHJZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000005424 photoluminescence Methods 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- KSFOVUSSGSKXFI-GAQDCDSVSA-N CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O Chemical compound CC1=C/2NC(\C=C3/N=C(/C=C4\N\C(=C/C5=N/C(=C\2)/C(C=C)=C5C)C(C=C)=C4C)C(C)=C3CCC(O)=O)=C1CCC(O)=O KSFOVUSSGSKXFI-GAQDCDSVSA-N 0.000 claims description 3
- HUVXQFBFIFIDDU-UHFFFAOYSA-N aluminum phthalocyanine Chemical compound [Al+3].C12=CC=CC=C2C(N=C2[N-]C(C3=CC=CC=C32)=N2)=NC1=NC([C]1C=CC=CC1=1)=NC=1N=C1[C]3C=CC=CC3=C2[N-]1 HUVXQFBFIFIDDU-UHFFFAOYSA-N 0.000 claims description 3
- VAJVGAQAYOAJQI-UHFFFAOYSA-N 3-[18-(2-carboxylatoethyl)-3,8,13,17-tetramethyl-22,23-dihydroporphyrin-21,24-diium-2-yl]propanoate Chemical compound N1C(C=C2C(C)=CC(N2)=CC=2C(=C(CCC(O)=O)C(=C3)N=2)C)=CC(C)=C1C=C1C(C)=C(CCC(O)=O)C3=N1 VAJVGAQAYOAJQI-UHFFFAOYSA-N 0.000 claims description 2
- NCAJWYASAWUEBY-UHFFFAOYSA-N 3-[20-(2-carboxyethyl)-9,14-diethyl-5,10,15,19-tetramethyl-21,22,23,24-tetraazapentacyclo[16.2.1.1^{3,6}.1^{8,11}.1^{13,16}]tetracosa-1(21),2,4,6(24),7,9,11,13,15,17,19-undecaen-4-yl]propanoic acid Chemical compound N1C2=C(C)C(CC)=C1C=C(N1)C(C)=C(CC)C1=CC(C(C)=C1CCC(O)=O)=NC1=CC(C(CCC(O)=O)=C1C)=NC1=C2 NCAJWYASAWUEBY-UHFFFAOYSA-N 0.000 claims description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims description 2
- 229910052801 chlorine Inorganic materials 0.000 claims description 2
- 239000000460 chlorine Substances 0.000 claims description 2
- HCIIFBHDBOCSAF-UHFFFAOYSA-N octaethylporphyrin Chemical compound N1C(C=C2C(=C(CC)C(C=C3C(=C(CC)C(=C4)N3)CC)=N2)CC)=C(CC)C(CC)=C1C=C1C(CC)=C(CC)C4=N1 HCIIFBHDBOCSAF-UHFFFAOYSA-N 0.000 claims description 2
- 229950003776 protoporphyrin Drugs 0.000 claims description 2
- YNHJECZULSZAQK-UHFFFAOYSA-N tetraphenylporphyrin Chemical compound C1=CC(C(=C2C=CC(N2)=C(C=2C=CC=CC=2)C=2C=CC(N=2)=C(C=2C=CC=CC=2)C2=CC=C3N2)C=2C=CC=CC=2)=NC1=C3C1=CC=CC=C1 YNHJECZULSZAQK-UHFFFAOYSA-N 0.000 claims description 2
- HPQNHLPQBFLGSH-UHFFFAOYSA-N COC1=CC=CC=C1C1=CC2=CC([N]3)=CC=C3C=C(C=C3)NC3=CC([N]3)=CC=C3C=C1N2 Chemical compound COC1=CC=CC=C1C1=CC2=CC([N]3)=CC=C3C=C(C=C3)NC3=CC([N]3)=CC=C3C=C1N2 HPQNHLPQBFLGSH-UHFFFAOYSA-N 0.000 claims 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 1
- 230000003213 activating effect Effects 0.000 claims 1
- 238000010235 multi-parametric assay Methods 0.000 claims 1
- IEQIEDJGQAUEQZ-UHFFFAOYSA-N phthalocyanine Chemical compound N1C(N=C2C3=CC=CC=C3C(N=C3C4=CC=CC=C4C(=N4)N3)=N2)=C(C=CC=C2)C2=C1N=C1C2=CC=CC=C2C4=N1 IEQIEDJGQAUEQZ-UHFFFAOYSA-N 0.000 claims 1
- 239000011701 zinc Substances 0.000 claims 1
- 229910052725 zinc Inorganic materials 0.000 claims 1
- 239000012491 analyte Substances 0.000 abstract description 4
- 239000012535 impurity Substances 0.000 abstract 2
- 238000004020 luminiscence type Methods 0.000 abstract 1
- 238000000295 emission spectrum Methods 0.000 description 8
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 6
- 229910052747 lanthanoid Inorganic materials 0.000 description 6
- 150000002602 lanthanoids Chemical class 0.000 description 6
- 238000003556 assay Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000000695 excitation spectrum Methods 0.000 description 5
- 239000011159 matrix material Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 235000017168 chlorine Nutrition 0.000 description 3
- 229960000956 coumarin Drugs 0.000 description 3
- 235000001671 coumarin Nutrition 0.000 description 3
- 150000004032 porphyrins Chemical class 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000012190 activator Substances 0.000 description 2
- 125000001309 chloro group Chemical class Cl* 0.000 description 2
- 229930002875 chlorophyll Natural products 0.000 description 2
- 235000019804 chlorophyll Nutrition 0.000 description 2
- 239000001752 chlorophylls and chlorophyllins Substances 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 230000005923 long-lasting effect Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000013081 microcrystal Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 239000002344 surface layer Substances 0.000 description 2
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 2
- NNJPGOLRFBJNIW-HNNXBMFYSA-N (-)-demecolcine Chemical compound C1=C(OC)C(=O)C=C2[C@@H](NC)CCC3=CC(OC)=C(OC)C(OC)=C3C2=C1 NNJPGOLRFBJNIW-HNNXBMFYSA-N 0.000 description 1
- 229910052692 Dysprosium Inorganic materials 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229910052693 Europium Inorganic materials 0.000 description 1
- RSPISYXLHRIGJD-UHFFFAOYSA-N OOOO Chemical compound OOOO RSPISYXLHRIGJD-UHFFFAOYSA-N 0.000 description 1
- 229910052772 Samarium Inorganic materials 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- 229910052771 Terbium Inorganic materials 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000005083 Zinc sulfide Substances 0.000 description 1
- 150000004036 bacteriochlorins Chemical class 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- GLNDAGDHSLMOKX-UHFFFAOYSA-N coumarin 120 Chemical compound C1=C(N)C=CC2=C1OC(=O)C=C2C GLNDAGDHSLMOKX-UHFFFAOYSA-N 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 229910021644 lanthanide ion Inorganic materials 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- SIWVEOZUMHYXCS-UHFFFAOYSA-N oxo(oxoyttriooxy)yttrium Chemical compound O=[Y]O[Y]=O SIWVEOZUMHYXCS-UHFFFAOYSA-N 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000000700 radioactive tracer Substances 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 229910052984 zinc sulfide Inorganic materials 0.000 description 1
- DRDVZXDWVBGGMH-UHFFFAOYSA-N zinc;sulfide Chemical compound [S-2].[Zn+2] DRDVZXDWVBGGMH-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Claims (2)
1. Biospesifik multiparametrisk bestämningsmetod, där man använder en blandning av pä förhand tillverkade, i olika kategorier indelade mikropartiklar som fast bärare representerande olika analyter, där mikropartiklar hörande 5 tili olika kategorier är belagda med olika biospecifika reagens, som binder olika analyter och där mikropartiklarna innehäller en eller flera olika fluorescerande färgämnen I\ i en eller flera koncentrationer beroende av mikropartikel-kategorin, i vilken metod man 10. blandar de tili olika kategorier hörande mikropartiklarna och tillsätter provet som skall analyseras tili blandningen och tillsätter med en fotoluminerande markör märkta biospesifika reagens tili blandningen, och - aktiverar de fluorescerande färgämnena Dk och de 15 fotoluminerande markörerna F, och - med en tidsfördröjd fluorimeter mäter styrkan för fluorescensen hos mikropartiklarnas färgämne Dk för bestämning av mikropartikelkategorin och styrkan för fotoluminescensen hos markören F för bestämning av 20 analytmängderna, kännetecknad därav, att man som fluorescerande föreningar Dk valt ett eller flera av de följande tetrapyrrolföreningsklassernas derivat: porfyriner, kloriner, bakteriokloriner, klorofyller, purpuriner, feoforbidider, ftalosyaniner och 25 naftalosyaniner, varvid de valda färgämnena Dk - har gemensam exciteringsväglängd för fluorescensen, och - har skilda smala frän varandra spektrometriskt lätt särskiljbara emissionsband, och - upplösningstiden för fluoresensens exciteringstillständ 30 hos dem är kort jämfört med upplösningstiden för emissionen hos den fotoluminerande markören F.
2. Metod enligt patentkravet 1 kännetecknad därav, att man som fluorescerande föreningar använder ett eller flera av 96367 de följande tetrapyrrolföreningarnas derivat: 1) deuteroporfyrin IX, 2) mesoporfyrin IX, 3) protoporfyrin (IX) dimetylester, 4) oktaetylporfin, 5) tetrafenylporfin, 6. tetra-(2-metoxi)-fenylporfin, 7) koproporfyrin 5 dimetylester klorin 8) koproporfyrin dimetylester bakterioklorin, 9) aluminiumftalosyanin, 10) zinkftalosyanin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI944865A FI96367C (sv) | 1994-10-17 | 1994-10-17 | Biospesifik bestämningsmetod |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FI944865A FI96367C (sv) | 1994-10-17 | 1994-10-17 | Biospesifik bestämningsmetod |
| FI944865 | 1994-10-17 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| FI944865A0 FI944865A0 (sv) | 1994-10-17 |
| FI96367B true FI96367B (sv) | 1996-02-29 |
| FI96367C FI96367C (sv) | 1996-06-10 |
Family
ID=8541601
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FI944865A FI96367C (sv) | 1994-10-17 | 1994-10-17 | Biospesifik bestämningsmetod |
Country Status (1)
| Country | Link |
|---|---|
| FI (1) | FI96367C (sv) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998015830A3 (en) * | 1996-10-04 | 1998-06-18 | Wallac Oy | Homogenous luminescence energy transfer assays |
-
1994
- 1994-10-17 FI FI944865A patent/FI96367C/sv active IP Right Grant
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998015830A3 (en) * | 1996-10-04 | 1998-06-18 | Wallac Oy | Homogenous luminescence energy transfer assays |
Also Published As
| Publication number | Publication date |
|---|---|
| FI944865A0 (sv) | 1994-10-17 |
| FI96367C (sv) | 1996-06-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Patent granted |
Owner name: SOINI, ERKKI JUHANI |
|
| BB | Publication of examined application |