FI942691A0 - Menetelmä tuottaa/erittää proteiinia transformoidun homeen avulla käyttämällä Aspergilluksen endoksylanaasi II-geenistä saatuja ekspressioita/erittämistä sääteleviä alueita - Google Patents

Menetelmä tuottaa/erittää proteiinia transformoidun homeen avulla käyttämällä Aspergilluksen endoksylanaasi II-geenistä saatuja ekspressioita/erittämistä sääteleviä alueita

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Publication number
FI942691A0
FI942691A0 FI942691A FI942691A FI942691A0 FI 942691 A0 FI942691 A0 FI 942691A0 FI 942691 A FI942691 A FI 942691A FI 942691 A FI942691 A FI 942691A FI 942691 A0 FI942691 A0 FI 942691A0
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FI
Finland
Prior art keywords
endoxylanase
expression
aspergillus
secretion
gene
Prior art date
Application number
FI942691A
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English (en)
Swedish (sv)
Other versions
FI942691A (fi
FI111958B (fi
Inventor
Robertus Johannes Gouka
Den Hondel Cornelis Antoni Van
Wouter Musters
Hein Stam
Johannes Maria A Verbakel
Original Assignee
Unilever Nv
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Publication date
Application filed by Unilever Nv filed Critical Unilever Nv
Publication of FI942691A publication Critical patent/FI942691A/fi
Publication of FI942691A0 publication Critical patent/FI942691A0/fi
Application granted granted Critical
Publication of FI111958B publication Critical patent/FI111958B/fi

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • C12N9/20Triglyceride splitting, e.g. by means of lipase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477Hemicellulases not provided in a preceding group
    • C12N9/248Xylanases
    • C12N9/2482Endo-1,4-beta-xylanase (3.2.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008Endo-1,4-beta-xylanase (3.2.1.8)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01031Beta-glucuronidase (3.2.1.31)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01032Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1-3-beta-xylanase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01136Glucuronoarabinoxylan endo-1,4-beta-xylanase (3.2.1.136), i.e. feraxanase or feraxan-endoxylanase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
FI942691A 1991-12-09 1994-06-08 Menetelmä tuottaa/erittää proteiinia transformoidun homeen avulla käyttämällä Aspergilluksen endoksylanaasi II-geenistä saatuja ekspressioita/erittämistä sääteleviä alueita FI111958B (fi)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
NL9102051 1991-12-09
NL9102051 1991-12-09
EP9202896 1992-12-09
PCT/EP1992/002896 WO1993012237A1 (en) 1991-12-09 1992-12-09 Process for producing/secreting a protein by a transformed mould using expression/secretion regulating regions derived from an aspergillus endoxylanase ii gene

Publications (3)

Publication Number Publication Date
FI942691A FI942691A (fi) 1994-06-08
FI942691A0 true FI942691A0 (fi) 1994-06-08
FI111958B FI111958B (fi) 2003-10-15

Family

ID=19860011

Family Applications (1)

Application Number Title Priority Date Filing Date
FI942691A FI111958B (fi) 1991-12-09 1994-06-08 Menetelmä tuottaa/erittää proteiinia transformoidun homeen avulla käyttämällä Aspergilluksen endoksylanaasi II-geenistä saatuja ekspressioita/erittämistä sääteleviä alueita

Country Status (12)

Country Link
US (1) US5705358A (fi)
EP (1) EP0672149B1 (fi)
JP (1) JP2866739B2 (fi)
AT (1) ATE204022T1 (fi)
AU (1) AU664223B2 (fi)
CA (1) CA2123416C (fi)
DE (1) DE69231995T2 (fi)
DK (1) DK0672149T3 (fi)
ES (1) ES2159519T3 (fi)
FI (1) FI111958B (fi)
WO (1) WO1993012237A1 (fi)
ZA (1) ZA929539B (fi)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5571697A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Texas Medical Center Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
IL108175A0 (en) * 1992-12-24 1994-04-12 Gist Brocades Nv Cloning and expression of xylanase B
ES2316882T3 (es) * 1993-03-10 2009-04-16 Novozymes A/S Enzimas de aspergillus aculeatus con una actividad xilanasa.
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
EP0870835A1 (en) * 1997-04-07 1998-10-14 Unilever N.V. Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus
JP4307563B2 (ja) * 1997-04-07 2009-08-05 ユニリーバー・ナームローゼ・ベンノートシャープ 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換
US6015703A (en) 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase
CA2345356C (en) * 1998-10-06 2012-10-02 Mark Aaron Emalfarb Transformation system in the field of filamentous fungal hosts
JP4834554B2 (ja) * 2003-11-06 2011-12-14 ジェネンコー・インターナショナル・インク 糸状菌におけるプロテアーゼ抑制剤及びその変異体の発現
EP1979476B1 (en) * 2006-01-23 2016-08-10 Novozymes A/S Polypeptides having lipase activity and polynucleotides encoding same
CN103451164A (zh) 2006-07-14 2013-12-18 诺维信股份有限公司 用于产生具有生物学活性的分泌型多肽的方法
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
CA2736661A1 (en) * 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
KR20200026878A (ko) 2017-06-06 2020-03-11 지머젠 인코포레이티드 균류 균주를 개량하기 위한 htp 게놈 공학 플랫폼
WO2019236848A1 (en) 2018-06-06 2019-12-12 Zymergen Inc. Manipulation of genes involved in signal transduction to control fungal morphology during fermentation and production
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1341226C (en) * 1988-08-16 2001-05-01 Wim Van Hartingsveldt Gene replacement as a tool for the construction of aspergillus strains
US5171672A (en) * 1989-08-31 1992-12-15 Allelix Biopharmaceuticals Inc. Recombinant DNA system for Aspergillus
EP0421919A3 (en) * 1989-09-02 1992-04-15 Ciba-Geigy Ag Polygalacturonase encoding fungal expression system
JP2995770B2 (ja) * 1989-12-15 1999-12-27 吉富製薬株式会社 異種蛋白質の製造方法
FI103206B1 (fi) * 1990-01-29 1999-05-14 Novartis Ag Uusi ilmentämisjärjestelmä sieniä varten
NL9001388A (nl) * 1990-06-19 1992-01-16 Unilever Nv Recombinant dna, cel die daarvan afgeleid dna bevat, enzym waarvoor het recombinant dna codeert en toepassingen daarvan.
NZ239083A (en) * 1990-07-24 1993-08-26 Gist Brocades Nv Endo-xylanase-containing silage composition

Also Published As

Publication number Publication date
EP0672149A1 (en) 1995-09-20
AU3158393A (en) 1993-07-19
FI942691A (fi) 1994-06-08
DE69231995T2 (de) 2002-04-04
DE69231995D1 (de) 2001-09-13
DK0672149T3 (da) 2001-11-05
US5705358A (en) 1998-01-06
ATE204022T1 (de) 2001-08-15
WO1993012237A1 (en) 1993-06-24
FI111958B (fi) 2003-10-15
ZA929539B (en) 1994-06-09
ES2159519T3 (es) 2001-10-16
CA2123416A1 (en) 1993-06-24
JPH07501453A (ja) 1995-02-16
JP2866739B2 (ja) 1999-03-08
EP0672149B1 (en) 2001-08-08
AU664223B2 (en) 1995-11-09
CA2123416C (en) 2003-05-20

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