FI91449C - Förfarande för bestämning av biologisk substans in situ - Google Patents

Förfarande för bestämning av biologisk substans in situ Download PDF

Info

Publication number
FI91449C
FI91449C FI924905A FI924905A FI91449C FI 91449 C FI91449 C FI 91449C FI 924905 A FI924905 A FI 924905A FI 924905 A FI924905 A FI 924905A FI 91449 C FI91449 C FI 91449C
Authority
FI
Finland
Prior art keywords
phosphorescence
fluorescence
reaction
reagent
situ
Prior art date
Application number
FI924905A
Other languages
English (en)
Finnish (fi)
Other versions
FI924905A0 (sv
FI91449B (sv
Inventor
Alexander Savitsky
Geley Ponomarev
Erkki Soini
Ilkka Hemmilae
Original Assignee
Wallac Oy
Alexander Savitsky
Geley Ponomarev
Erkki Soini
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wallac Oy, Alexander Savitsky, Geley Ponomarev, Erkki Soini filed Critical Wallac Oy
Priority to FI924905A priority Critical patent/FI91449C/sv
Publication of FI924905A0 publication Critical patent/FI924905A0/sv
Priority to PCT/FI1993/000414 priority patent/WO1994010568A1/en
Application granted granted Critical
Publication of FI91449B publication Critical patent/FI91449B/sv
Publication of FI91449C publication Critical patent/FI91449C/sv

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Claims (6)

1. In situ -metod f6r kvantitativ eller kvalitativ bestamning av ett biologiskt Mmne med hjalp av en biospecifik bindningsreaktion, varvid ett reagens, som Mr markt med ett fotoluminescerande metalloporfyrin antingen 5 kovalent eller indirekt med hjMlp av till reagenset kopplade brobildande antikroppar, varvid namnda reagens omsattes med det biologiska reagerande Mmnet kannetecknad darav, att mMtningen av fotoluminescensemissionen utfors på en yta under fdrhållanden, som ar Mgnade att minska både a) 10 frigdringen av det fotoluminescerande markningsMmnet eller av reagenset mMrkt med det fotoluminescerande markningsMmnet och b) utslMckningen av det fotoluminescerande metalloporfyrinmårkningsMmnet, varvid matningen utfors antingen 15 - i ett torrt prov, eller - under en vattenhaltig detergentlosning, från vilken syre avlagsnats, eller - i ett polymeriserande vattenfritt tackmaterial.
2. Metod enligt patentkravet 1 kMnnetecknad darav, att man 20 anvander ett fosforescerande metalloporfyrin.
3. Metod enligt något av patentkraven 1-2 kannetecknad dårav, att den i metalloporfyrinet befintliga kelaterade metallen har utvalts från en grupp i vilken ingår Pd, Pt, Y, Lu, Sn, Zn, Al eller Yb.
4. Metod enligt något av patentkraven 1-3 kannetecknad darav, att den biospecifika bindningsreaktionen a) baserar sig på antikroppar, b) ar en receptorbindningsbestamning eller c) Mr en bestamning som grundar sig på DNA- eller RNA-prober.
5. Metod enligt något av patentkraven 1-4 kannetecknad dSrav, att ytan ar en inom immunbestamnig anvånd plastyta, ett inom immunbestMmning eller DNA-proberbestamning anvant 91449 membran eller filter, en våvnadssnitsel eller en cellyta.
6. Metod enligt något av patentkraven 1-5 kannetecknad darav, att fotoluminescensen, som ar fosforescens eller tidsfordrojd fluorescens, mats med en tidsfordrojd 5 fluorimeter, ett fluorescensmikroskop, bildanalys eller en svepfluorimeter.
FI924905A 1992-10-29 1992-10-29 Förfarande för bestämning av biologisk substans in situ FI91449C (sv)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FI924905A FI91449C (sv) 1992-10-29 1992-10-29 Förfarande för bestämning av biologisk substans in situ
PCT/FI1993/000414 WO1994010568A1 (en) 1992-10-29 1993-10-12 METHOD FOR THE DETERMINATION OF A BIOLOGICAL SUBSTANCE $i(IN SITU)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI924905A FI91449C (sv) 1992-10-29 1992-10-29 Förfarande för bestämning av biologisk substans in situ
FI924905 1992-10-29

Publications (3)

Publication Number Publication Date
FI924905A0 FI924905A0 (sv) 1992-10-29
FI91449B FI91449B (sv) 1994-03-15
FI91449C true FI91449C (sv) 1994-06-27

Family

ID=8536128

Family Applications (1)

Application Number Title Priority Date Filing Date
FI924905A FI91449C (sv) 1992-10-29 1992-10-29 Förfarande för bestämning av biologisk substans in situ

Country Status (2)

Country Link
FI (1) FI91449C (sv)
WO (1) WO1994010568A1 (sv)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10138267B2 (en) 2015-04-06 2018-11-27 Hunan Skyworld Biotechnologies Co. Ltd. Bioconjugates of heterocyclic compounds

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0350948B1 (en) * 1988-07-14 1998-03-18 Toyohakka Kogyo Kabushiki Kaisha Porphyrin derivatives

Also Published As

Publication number Publication date
FI924905A0 (sv) 1992-10-29
WO1994010568A1 (en) 1994-05-11
FI91449B (sv) 1994-03-15

Similar Documents

Publication Publication Date Title
CN102072891B (zh) 金属修饰的光子晶体生物检测薄膜及其制备方法和用途
US6306589B1 (en) Biological assays for analyte detection
Fähnrich et al. Recent applications of electrogenerated chemiluminescence in chemical analysis
US7332344B2 (en) Luminescence assays
US7517701B2 (en) Luminescent lanthanide complexes
US20090227043A1 (en) Fluorescence Resonance Energy Transfer Assay Based on Modified Solid Surface
AU686490B2 (en) Long lifetime anisotropy (polarization) probes for clinical chemistry, immunoassays, affinity assays and biomedical research
Mayr et al. Dual lifetime referenced optical sensor membrane for the determination of copper (II) ions
JPH07502820A (ja) 生体特異的多変数検定法
JPH10307141A (ja) プラズモン共鳴および蛍光検出を用いた生体分子相互作用の同時検出法
US20180074047A1 (en) Detecting target molecules in a sample
JP6010110B2 (ja) 発光重合体の反復増幅
EP3295174B1 (en) Method for re-using test probe and reagents in an immunoassay
Turel et al. Direct UV-LED lifetime pH sensor based on a semi-permeable sol–gel membrane immobilized luminescent Eu3+ chelate complex
CN110268247A (zh) 单分子水平的蛋白质-蛋白质相互作用的测量
RU2379691C1 (ru) Способ многоаналитного иммуноанализа с использованием микрочастиц
Matsumoto Recent developments in lanthanide chelates as luminescent labels for biomedical analyses
FI91449C (sv) Förfarande för bestämning av biologisk substans in situ
US7629179B2 (en) Surfaces coated with target-induced fluorescent compounds for detection of target elements
Soper et al. Molecular fluorescence, phosphorescence, and chemiluminescence spectrometry
Crystall et al. Time resolved evanescent wave induced fluorescence measurements of surface adsorbed bovine serum albumin
Soini et al. Two-photon fluorescence excitation in detection of biomolecules
WO1997020213A1 (en) Luminescent probes for protein detection
Savitsky et al. Solid surface measurement of room temperature phosphorescence of Pd-coproporphyrin and its application for time-resolved microscopy
RU2197732C1 (ru) Способ обнаружения и идентификации клеток микроорганизмов и вирусов

Legal Events

Date Code Title Description
BB Publication of examined application