FI116838B - Tyypittömän haemophiluksen molekyylipainoltaan suurta pintaproteiinia koodaava geeni ja geeniryhmä - Google Patents
Tyypittömän haemophiluksen molekyylipainoltaan suurta pintaproteiinia koodaava geeni ja geeniryhmä Download PDFInfo
- Publication number
- FI116838B FI116838B FI944273A FI944273A FI116838B FI 116838 B FI116838 B FI 116838B FI 944273 A FI944273 A FI 944273A FI 944273 A FI944273 A FI 944273A FI 116838 B FI116838 B FI 116838B
- Authority
- FI
- Finland
- Prior art keywords
- protein
- gene
- sequence
- hmw1
- encoded
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 150
- 241000606790 Haemophilus Species 0.000 title claims description 17
- 108010052285 Membrane Proteins Proteins 0.000 title abstract description 5
- 102000018697 Membrane Proteins Human genes 0.000 title abstract description 3
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 78
- 241000606768 Haemophilus influenzae Species 0.000 claims abstract description 18
- 101100017567 Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) hlp1 gene Proteins 0.000 claims abstract description 8
- 101100395360 Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) hlp3 gene Proteins 0.000 claims abstract description 8
- 229940047650 haemophilus influenzae Drugs 0.000 claims abstract description 8
- 101150051207 hmw3 gene Proteins 0.000 claims abstract description 8
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 20
- 239000002773 nucleotide Substances 0.000 claims description 17
- 125000003729 nucleotide group Chemical group 0.000 claims description 17
- 239000012634 fragment Substances 0.000 claims description 15
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 14
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 11
- 239000000427 antigen Substances 0.000 claims description 10
- 102000036639 antigens Human genes 0.000 claims description 10
- 108091007433 antigens Proteins 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 108020004414 DNA Proteins 0.000 claims description 9
- 229960005486 vaccine Drugs 0.000 claims description 8
- 208000037386 Typhoid Diseases 0.000 claims description 6
- 201000008297 typhoid fever Diseases 0.000 claims description 6
- 206010033078 Otitis media Diseases 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 230000014509 gene expression Effects 0.000 claims description 5
- 230000001681 protective effect Effects 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 150000004676 glycans Chemical class 0.000 claims description 4
- 229920001282 polysaccharide Polymers 0.000 claims description 4
- 239000005017 polysaccharide Substances 0.000 claims description 4
- 238000012217 deletion Methods 0.000 claims description 3
- 230000037430 deletion Effects 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 230000035772 mutation Effects 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 238000005516 engineering process Methods 0.000 claims description 2
- 230000001900 immune effect Effects 0.000 claims description 2
- 230000036039 immunity Effects 0.000 claims description 2
- 201000009890 sinusitis Diseases 0.000 claims description 2
- 230000002194 synthesizing effect Effects 0.000 claims description 2
- 101150109178 M1 gene Proteins 0.000 claims 1
- 229940045808 haemophilus influenzae type b Drugs 0.000 claims 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims 1
- 230000002163 immunogen Effects 0.000 abstract 1
- 101150045401 hmw1 gene Proteins 0.000 description 58
- 101150107814 hmw2 gene Proteins 0.000 description 39
- 101100017570 Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) hlp2 gene Proteins 0.000 description 38
- 239000013612 plasmid Substances 0.000 description 20
- 241000588724 Escherichia coli Species 0.000 description 17
- 210000004027 cell Anatomy 0.000 description 14
- 101710154643 Filamentous hemagglutinin Proteins 0.000 description 11
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 9
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 9
- 229940027941 immunoglobulin g Drugs 0.000 description 8
- 210000002966 serum Anatomy 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 108020004705 Codon Proteins 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 238000003556 assay Methods 0.000 description 5
- 230000029087 digestion Effects 0.000 description 5
- 229930027917 kanamycin Natural products 0.000 description 5
- 229960000318 kanamycin Drugs 0.000 description 5
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 5
- 229930182823 kanamycin A Natural products 0.000 description 5
- 238000011144 upstream manufacturing Methods 0.000 description 5
- 241000588832 Bordetella pertussis Species 0.000 description 4
- 241000283707 Capra Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 238000001962 electrophoresis Methods 0.000 description 4
- 210000002919 epithelial cell Anatomy 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 230000009257 reactivity Effects 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 229910019142 PO4 Inorganic materials 0.000 description 3
- 238000012300 Sequence Analysis Methods 0.000 description 3
- 206010006451 bronchitis Diseases 0.000 description 3
- 239000013599 cloning vector Substances 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 210000000959 ear middle Anatomy 0.000 description 3
- 239000002054 inoculum Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
- 239000010452 phosphate Substances 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 238000012805 post-processing Methods 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 238000001262 western blot Methods 0.000 description 3
- 206010010741 Conjunctivitis Diseases 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 241001529936 Murinae Species 0.000 description 2
- 239000000020 Nitrocellulose Substances 0.000 description 2
- 108700026244 Open Reading Frames Proteins 0.000 description 2
- 208000005141 Otitis Diseases 0.000 description 2
- 201000005702 Pertussis Diseases 0.000 description 2
- 229920002684 Sepharose Polymers 0.000 description 2
- 108091081024 Start codon Proteins 0.000 description 2
- 102400000368 Surface protein Human genes 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000853 adhesive Substances 0.000 description 2
- 230000001070 adhesive effect Effects 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 239000002775 capsule Substances 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 230000002950 deficient Effects 0.000 description 2
- 208000019258 ear infection Diseases 0.000 description 2
- 239000013613 expression plasmid Substances 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000002523 gelfiltration Methods 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 238000002649 immunization Methods 0.000 description 2
- 238000003119 immunoblot Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229920001220 nitrocellulos Polymers 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000012723 sample buffer Substances 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000001488 sodium phosphate Substances 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 101150055869 25 gene Proteins 0.000 description 1
- HQFLTUZKIRYQSP-UHFFFAOYSA-N 3-ethyl-2h-1,3-benzothiazole-6-sulfonic acid Chemical compound OS(=O)(=O)C1=CC=C2N(CC)CSC2=C1 HQFLTUZKIRYQSP-UHFFFAOYSA-N 0.000 description 1
- 108020005029 5' Flanking Region Proteins 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 241000588807 Bordetella Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000700114 Chinchillidae Species 0.000 description 1
- 108010060123 Conjugate Vaccines Proteins 0.000 description 1
- 241000699802 Cricetulus griseus Species 0.000 description 1
- 101710173353 Cytotoxicity-associated immunodominant antigen Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- 241001198387 Escherichia coli BL21(DE3) Species 0.000 description 1
- 101001065501 Escherichia phage MS2 Lysis protein Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000958664 Homo sapiens Nucleus accumbens-associated protein 1 Proteins 0.000 description 1
- 101000631760 Homo sapiens Sodium channel protein type 1 subunit alpha Proteins 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 201000009906 Meningitis Diseases 0.000 description 1
- 101100005280 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cat-3 gene Proteins 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- 102100028910 Sodium channel protein type 1 subunit alpha Human genes 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 101500022167 Youcai mosaic virus Replicase small subunit Proteins 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 230000002788 anti-peptide Effects 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000019219 chocolate Nutrition 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 229940031670 conjugate vaccine Drugs 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 206010015037 epilepsy Diseases 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- BTIJJDXEELBZFS-QDUVMHSLSA-K hemin Chemical compound CC1=C(CCC(O)=O)C(C=C2C(CCC(O)=O)=C(C)\C(N2[Fe](Cl)N23)=C\4)=N\C1=C/C2=C(C)C(C=C)=C3\C=C/1C(C)=C(C=C)C/4=N\1 BTIJJDXEELBZFS-QDUVMHSLSA-K 0.000 description 1
- 229940025294 hemin Drugs 0.000 description 1
- 108010037896 heparin-binding hemagglutinin Proteins 0.000 description 1
- 229940098197 human immunoglobulin g Drugs 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 150000002605 large molecules Chemical class 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000001926 lymphatic effect Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 102000013415 peroxidase activity proteins Human genes 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000009145 protein modification Effects 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229940126577 synthetic vaccine Drugs 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/285—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pasteurellaceae (F), e.g. Haemophilus influenza
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/851—Haemophilus
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Communicable Diseases (AREA)
- Biophysics (AREA)
- Pharmacology & Pharmacy (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Veterinary Medicine (AREA)
- Biotechnology (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Epidemiology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB929205704A GB9205704D0 (en) | 1992-03-16 | 1992-03-16 | High molecular weight membrane proteins of non-typeable haemophilus |
GB9205704 | 1992-03-16 | ||
PCT/US1993/002166 WO1993019090A1 (en) | 1992-03-16 | 1993-03-16 | High molecular weight surface proteines of non-typeable haemophilus |
US9302166 | 1993-03-16 |
Publications (3)
Publication Number | Publication Date |
---|---|
FI944273A0 FI944273A0 (fi) | 1994-09-15 |
FI944273A FI944273A (fi) | 1994-11-15 |
FI116838B true FI116838B (fi) | 2006-03-15 |
Family
ID=10712229
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
FI944273A FI116838B (fi) | 1992-03-16 | 1994-09-15 | Tyypittömän haemophiluksen molekyylipainoltaan suurta pintaproteiinia koodaava geeni ja geeniryhmä |
Country Status (17)
Country | Link |
---|---|
US (2) | US5549897A (ru) |
EP (1) | EP0632814B1 (ru) |
JP (1) | JP2810235B2 (ru) |
KR (1) | KR100219126B1 (ru) |
AT (1) | ATE222955T1 (ru) |
AU (1) | AU669360B2 (ru) |
BR (1) | BR9306109A (ru) |
CA (1) | CA2131837C (ru) |
DE (1) | DE69332243T2 (ru) |
DK (1) | DK0632814T3 (ru) |
ES (1) | ES2183812T3 (ru) |
FI (1) | FI116838B (ru) |
GB (1) | GB9205704D0 (ru) |
NO (1) | NO316623B1 (ru) |
PT (1) | PT632814E (ru) |
RU (1) | RU2157816C2 (ru) |
WO (1) | WO1993019090A1 (ru) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020164354A1 (en) * | 1998-09-30 | 2002-11-07 | Barenkamp Stephen J. | High molecular weight surface proteins of non-typeable haemphilus |
US5977336A (en) * | 1993-03-16 | 1999-11-02 | St. Louis University | High molecular weight surface proteins of non-typeable haemophilus |
US5869065A (en) * | 1993-03-16 | 1999-02-09 | Board Of Trustees Of The Leland Stanford Junior University | High molecular weight surface proteins of non-typeable haemophilus |
US5834187A (en) * | 1994-07-19 | 1998-11-10 | Bactex, Inc. | Sequence and analysis of LKP pilin structural genes and the LKP pili operon of nontypable Haemophilus influenzae |
US5643725A (en) * | 1994-07-19 | 1997-07-01 | American Cyanamid Company | Sequence and analysis of LKP pilin structural genes and the LKP pili operon of nontypable haemophilus influenzae |
ATE315648T1 (de) * | 1994-07-19 | 2006-02-15 | Wyeth Corp | Haemophilus influenzae (nt hi) lkp tip adhesin protein und nukleinsäure |
US5968769A (en) * | 1994-07-19 | 1999-10-19 | American Cyanamid Co. And Bactex, Inc. | Sequence and analysis of LKP pilin structural genes and the LKP pili operon of nontypable Haemophilus influenzae |
US6245337B1 (en) * | 1994-08-25 | 2001-06-12 | Washington University | Haemophilus adherence and penetration proteins |
US6355450B1 (en) | 1995-04-21 | 2002-03-12 | Human Genome Sciences, Inc. | Computer readable genomic sequence of Haemophilus influenzae Rd, fragments thereof, and uses thereof |
US6468765B1 (en) * | 1995-04-21 | 2002-10-22 | Human Genome Sciences, Inc. | Selected Haemophilus influenzae Rd polynucleotides and polypeptides |
WO1999016473A1 (en) * | 1997-05-19 | 1999-04-08 | Duke University | Lipid a 4' kinase |
US6432669B1 (en) | 1998-10-07 | 2002-08-13 | Aventis Pasteur Limited | Protective recombinant Haemophilus influenzae high molecular weight proteins |
NZ511360A (en) * | 1998-10-07 | 2004-01-30 | Aventis Pasteur | Protective recombinant haemophilus influenzae high molecular weight proteins |
US6974581B1 (en) * | 1998-12-15 | 2005-12-13 | Aventis Pasteur Limited | Multi-component vaccine comprising at least two antigens from haemophilus influenzae to protect against disease |
GB9904183D0 (en) * | 1999-02-24 | 1999-04-14 | Smithkline Beecham Biolog | Novel compounds |
US7785609B1 (en) | 1999-03-16 | 2010-08-31 | Sanofi Pasteur Limited | Recombinant Haemophilus influenzae adhesin proteins |
CN1374905A (zh) | 1999-10-29 | 2002-10-16 | 岛屿涂层有限公司 | 刻花辊及其加工方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9224584D0 (en) | 1992-11-23 | 1993-01-13 | Connaught Lab | Use of outer membrane protein d15 and its peptides as vaccine against haempohilus influenzae diseases |
GB9304399D0 (en) * | 1993-03-04 | 1993-04-21 | Smithkline Beecham Biolog | Novel process |
US5869065A (en) * | 1993-03-16 | 1999-02-09 | Board Of Trustees Of The Leland Stanford Junior University | High molecular weight surface proteins of non-typeable haemophilus |
-
1992
- 1992-03-16 GB GB929205704A patent/GB9205704D0/en active Pending
-
1993
- 1993-03-16 KR KR1019940703224A patent/KR100219126B1/ko not_active IP Right Cessation
- 1993-03-16 ES ES93908295T patent/ES2183812T3/es not_active Expired - Lifetime
- 1993-03-16 EP EP93908295A patent/EP0632814B1/en not_active Expired - Lifetime
- 1993-03-16 AU AU39168/93A patent/AU669360B2/en not_active Expired
- 1993-03-16 WO PCT/US1993/002166 patent/WO1993019090A1/en active IP Right Grant
- 1993-03-16 JP JP5516604A patent/JP2810235B2/ja not_active Expired - Lifetime
- 1993-03-16 BR BR9306109A patent/BR9306109A/pt not_active IP Right Cessation
- 1993-03-16 DE DE69332243T patent/DE69332243T2/de not_active Expired - Lifetime
- 1993-03-16 AT AT93908295T patent/ATE222955T1/de active
- 1993-03-16 PT PT93908295T patent/PT632814E/pt unknown
- 1993-03-16 CA CA002131837A patent/CA2131837C/en not_active Expired - Lifetime
- 1993-03-16 US US08/038,682 patent/US5549897A/en not_active Expired - Lifetime
- 1993-03-16 RU RU94042726/13A patent/RU2157816C2/ru active
- 1993-03-16 DK DK93908295T patent/DK0632814T3/da active
-
1994
- 1994-09-15 NO NO19943431A patent/NO316623B1/no not_active IP Right Cessation
- 1994-09-15 FI FI944273A patent/FI116838B/fi not_active IP Right Cessation
-
1996
- 1996-09-25 US US08/719,641 patent/US6218141B1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
AU669360B2 (en) | 1996-06-06 |
ES2183812T3 (es) | 2003-04-01 |
DE69332243D1 (de) | 2002-10-02 |
CA2131837C (en) | 2002-02-12 |
DE69332243T2 (de) | 2003-04-30 |
NO943431D0 (no) | 1994-09-15 |
WO1993019090A1 (en) | 1993-09-30 |
FI944273A (fi) | 1994-11-15 |
GB9205704D0 (en) | 1992-04-29 |
BR9306109A (pt) | 1997-11-18 |
CA2131837A1 (en) | 1993-09-17 |
ATE222955T1 (de) | 2002-09-15 |
NO316623B1 (no) | 2004-03-15 |
KR950700936A (ko) | 1995-02-20 |
EP0632814B1 (en) | 2002-08-28 |
US5549897A (en) | 1996-08-27 |
US6218141B1 (en) | 2001-04-17 |
KR100219126B1 (ko) | 1999-10-01 |
AU3916893A (en) | 1993-10-21 |
DK0632814T3 (da) | 2002-10-07 |
NO943431L (no) | 1994-11-10 |
EP0632814A1 (en) | 1995-01-11 |
RU2157816C2 (ru) | 2000-10-20 |
JPH07506248A (ja) | 1995-07-13 |
PT632814E (pt) | 2003-01-31 |
JP2810235B2 (ja) | 1998-10-15 |
FI944273A0 (fi) | 1994-09-15 |
EP0632814A4 (en) | 1996-03-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
FI116838B (fi) | Tyypittömän haemophiluksen molekyylipainoltaan suurta pintaproteiinia koodaava geeni ja geeniryhmä | |
Barenkamp et al. | Identification of a second family of high‐molecular‐weight adhesion proteins expressed by non‐typable Haemophilus influenzae | |
JP3771265B2 (ja) | モラクセラ属(Moraxella)の高分子量主要外膜タンパク | |
JP4109939B2 (ja) | 髄膜炎菌(Neisseriameningitidis)のプロテイナーゼK抵抗性表面蛋白質 | |
US5173294A (en) | Dna probe for the identification of haemophilus influenzae | |
US5932705A (en) | Methods and compositions for the treatment and diagnosis of shipping fever | |
US5766608A (en) | DNA molecules which encode the fimbrin protein of Haemophilus influenzae | |
CA2040544C (en) | Actinobacillus pleuropneumoniae subunit vaccine | |
EP0656014B1 (en) | Protein rib, a cell surface protein that confers immunity to many strains of the group b streptococcus; process for purification of the protein, reagent kit and pharmaceutical composition | |
KR960016208B1 (ko) | 인플루엔자균(Haemophilus influenzae)의 백신 및 진단방법. | |
EP0432220B1 (en) | Vaccines and diagnostic assays for haemophilus influenzae | |
US5869065A (en) | High molecular weight surface proteins of non-typeable haemophilus | |
JP2001503602A (ja) | 非共通形態型好血菌属の高分子量表面タンパク質 | |
US5876733A (en) | High molecular weight surface proteins of non-typeable haemophilus | |
US20050053618A1 (en) | High molecular weight surface proteins of non-typeable Haemophilus | |
NZ272660A (en) | Pasteurella multocida antigens, antibodies, their production and use | |
Chin | The Expression of Haemophilus Influenzae Type B Porin and Porin Deletions in the Baculovirus Expression Vector System |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG | Patent granted |
Ref document number: 116838 Country of ref document: FI |
|
MA | Patent expired |