FI102295B1 - Molecular cloning and expression of genes encoding lipolytic enzymes - Google Patents

Molecular cloning and expression of genes encoding lipolytic enzymes

Info

Publication number
FI102295B1
FI102295B1 FI895574A FI895574A FI102295B1 FI 102295 B1 FI102295 B1 FI 102295B1 FI 895574 A FI895574 A FI 895574A FI 895574 A FI895574 A FI 895574A FI 102295 B1 FI102295 B1 FI 102295B1
Authority
FI
Finland
Prior art keywords
expression
molecular cloning
genes encoding
lipolytic enzymes
encoding lipolytic
Prior art date
Application number
FI895574A
Other languages
Finnish (fi)
Swedish (sv)
Other versions
FI895574A0 (en
FI102295B (en
Inventor
Peter Michael Andreoli
Maria Mathilde Josephina Cox
Farrokh Farin
Suzanne Wohlfarth-Rippel
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Publication of FI895574A0 publication Critical patent/FI895574A0/en
Application granted granted Critical
Publication of FI102295B1 publication Critical patent/FI102295B1/en
Publication of FI102295B publication Critical patent/FI102295B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Novel microbial host strains are provided which are transformed by a vector molecule comprising a DNA fragment encoding a lipolytic enzyme and a marker for selection, capable of producing active lipase. Said DNA fragment is preferably derived from a Pseudomonas species.
FI895574A 1988-03-25 1989-11-22 Molecular cloning and expression of genes encoding lipolytic enzymes FI102295B (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
EP88200572 1988-03-25
EP88200572 1988-03-25
EP88201982 1988-09-12
EP88201982 1988-09-12
EP8900342 1989-03-28
PCT/EP1989/000342 WO1989009263A1 (en) 1988-03-25 1989-03-28 Molecular cloning and expression of genes encoding lipolytic enzymes

Publications (3)

Publication Number Publication Date
FI895574A0 FI895574A0 (en) 1989-11-22
FI102295B1 true FI102295B1 (en) 1998-11-13
FI102295B FI102295B (en) 1998-11-13

Family

ID=26115007

Family Applications (1)

Application Number Title Priority Date Filing Date
FI895574A FI102295B (en) 1988-03-25 1989-11-22 Molecular cloning and expression of genes encoding lipolytic enzymes

Country Status (8)

Country Link
JP (1) JP3029435B2 (en)
AT (1) ATE153067T1 (en)
AU (1) AU632472B2 (en)
DE (1) DE68928038T3 (en)
DK (1) DK175634B1 (en)
FI (1) FI102295B (en)
NO (1) NO307304B1 (en)
WO (1) WO1989009263A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5278066A (en) * 1985-08-09 1994-01-11 Gist-Brocades Nv Molecular cloning and expression of gene encoding lipolytic enzyme
US5227300A (en) * 1989-03-16 1993-07-13 Olin Corporation Identification, characterization and method of production of a novel microbial lipase
US5658871A (en) * 1989-07-07 1997-08-19 Lever Brothers Company, Division Of Conopco, Inc. Microbial lipase muteins and detergent compositions comprising same
ATE169678T1 (en) * 1991-05-01 1998-08-15 Novo Nordisk As STABILIZED ENZYMES
JP2859520B2 (en) * 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ Lipase, microorganism producing the same, method for producing lipase, and detergent composition containing lipase
GB0030877D0 (en) 2000-12-18 2001-01-31 Unilever Plc Enhancement of air bleaching catalysts
US20030051836A1 (en) 2001-05-21 2003-03-20 Novozymes A/S Enzymatic hydrolysis of a polymer comprising vinyl acetate monomer
US10087401B2 (en) 2012-03-16 2018-10-02 Monosol, Llc Water soluble compositions incorporating enzymes, and method of making same

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0697997B2 (en) * 1985-08-09 1994-12-07 ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ New enzymatic detergent additive
GB2191491B (en) * 1986-04-25 1990-12-05 Labofina Sa Dna segment coding for a specific lipase, vectors for the expression thereof, microorganisms transformed by these vectors and use of these microorganisms for
AU7901387A (en) * 1986-11-19 1988-06-09 Genencor Inc. Hydrolase from pseudomonas

Also Published As

Publication number Publication date
AU632472B2 (en) 1993-01-07
FI895574A0 (en) 1989-11-22
JP3029435B2 (en) 2000-04-04
DE68928038D1 (en) 1997-06-19
DK175634B1 (en) 2004-12-27
DE68928038T3 (en) 2002-09-12
AU3294789A (en) 1989-10-16
NO307304B1 (en) 2000-03-13
ATE153067T1 (en) 1997-05-15
WO1989009263A1 (en) 1989-10-05
DK591989A (en) 1989-11-24
JPH03500845A (en) 1991-02-28
NO894571D0 (en) 1989-11-16
NO894571L (en) 1989-11-16
DE68928038T2 (en) 1997-11-13
DK591989D0 (en) 1989-11-24
FI102295B (en) 1998-11-13

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FG Patent granted

Owner name: GENENCOR INTERNATIONAL INC.

MA Patent expired