KR930000687A - Structure and Expression Characteristics of Highly Active Amplified Promoter Gene Isolated from Chromosome DNA of Alkali-Resistant Bacillus - Google Patents

Structure and Expression Characteristics of Highly Active Amplified Promoter Gene Isolated from Chromosome DNA of Alkali-Resistant Bacillus Download PDF

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KR930000687A
KR930000687A KR1019910009779A KR910009779A KR930000687A KR 930000687 A KR930000687 A KR 930000687A KR 1019910009779 A KR1019910009779 A KR 1019910009779A KR 910009779 A KR910009779 A KR 910009779A KR 930000687 A KR930000687 A KR 930000687A
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alkali
promoter gene
highly active
expression characteristics
chromosome dna
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KR1019910009779A
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KR930010772B1 (en
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김진만
배동훈
오두환
박영서
박희경
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유주현
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

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Abstract

내용 없음No content

Description

알칼리 내성 바실러스속의 염색체 DNA로부터 분리된 고활성 중복 프로모터 유전자의 구조 및 발현 특성Structure and Expression Characteristics of Highly Active Double Promoter Gene Isolated from Chromosome DNA of Alkali-resistant Bacillus

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 플라스미드 pYJ에 삽입된 0.92 kb 프로모터 단면의 유전자 염기배열 및 RNA 중합효소를 특이 인식 프로모터의 위치를 표시한 도해이다.1 is a diagram showing the positions of gene sequencing and RNA polymerase specific recognition promoters of the 0.92 kb promoter cross-section inserted into the plasmid pYJ.

제2도는 플라스미드 pYJ로부터 제한효소 부위를 이용하여 분획된 유도체를 pYJ △f1, △f2, △f3, △f4 및 △f5를 제조해 나가는 과정에 대한 설명도이다.2 is an explanatory diagram of a process for preparing pYJ Δf1, Δf2, Δf3, Δf4, and Δf5 from derivatives fractionated from the plasmid pYJ using restriction enzyme sites.

제3도는 플라스미드 pYJ에 존재하는 프로모터들의 분획에 의하여 제조된 유도체들의 설명도이다.3 is an explanatory diagram of derivatives prepared by fractionation of promoters present in plasmid pYJ.

Claims (2)

플라스미드 pYJ에 삽입된 호알칼이성 바실러스 스페시스 YA-14유래 0.92kb BamHi-Sall DNA 단편의 염기배열과 동일한 염기배열 또는 일부를 갖는 프로모터 유전자와 이를 함유하는 미생물.A promoter gene having the same nucleotide sequence or part as the nucleotide sequence of a kal-14 Bacillus spesis YA-14-derived 0.92 kb BamHi-Sall DNA fragment inserted into plasmid pYJ, and a microorganism containing the same. 플라스미드 pYJ △f1 또는 그 유도체들의 프로모터에 미생물 및 동식물 유래의 유용 유전자를 결합시켜 효소 및 단백질등을 대량생산하는 방법.A method of mass-producing enzymes and proteins by binding useful genes derived from microorganisms and plants to promoters of plasmid pYJ Δf1 or derivatives thereof. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019910009779A 1991-06-14 1991-06-14 Promoter & plasmid from bacillus sp KR930010772B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019910009779A KR930010772B1 (en) 1991-06-14 1991-06-14 Promoter & plasmid from bacillus sp

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Application Number Priority Date Filing Date Title
KR1019910009779A KR930010772B1 (en) 1991-06-14 1991-06-14 Promoter & plasmid from bacillus sp

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KR930000687A true KR930000687A (en) 1993-01-15
KR930010772B1 KR930010772B1 (en) 1993-11-10

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