ES8707564A1 - Un metodo para obtener expresion de una secuencia de codificacion deseada en celulas de mamifero - Google Patents

Un metodo para obtener expresion de una secuencia de codificacion deseada en celulas de mamifero

Info

Publication number
ES8707564A1
ES8707564A1 ES551913A ES551913A ES8707564A1 ES 8707564 A1 ES8707564 A1 ES 8707564A1 ES 551913 A ES551913 A ES 551913A ES 551913 A ES551913 A ES 551913A ES 8707564 A1 ES8707564 A1 ES 8707564A1
Authority
ES
Spain
Prior art keywords
expression
promoter
produced
desired protein
expression system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES551913A
Other languages
English (en)
Other versions
ES551913A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biotechnology Research Partners Ltd
Original Assignee
Biotechnology Research Partners Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotechnology Research Partners Ltd filed Critical Biotechnology Research Partners Ltd
Publication of ES551913A0 publication Critical patent/ES551913A0/es
Publication of ES8707564A1 publication Critical patent/ES8707564A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6486—Renin (3.4.23.15)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/775—Apolipopeptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/785—Alveolar surfactant peptides; Pulmonary surfactant peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23015—Renin (3.4.23.15)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cardiology (AREA)
  • Physics & Mathematics (AREA)
  • Pulmonology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

METODO PARA LA OBTENCION DE LA EXPRESION DE SECUENCIAS DE GEN DESEADAS EN CELULAS DE MAMIFERO. CONSISTE EN TRANSFORMAR LAS CELULAS EN UNA SECUENCIA DE ADN QUE COMPRENDE EL PROMOTOR METALOTIONEINA-II (MT-II) UNIDO OPERATIVAMENTE A DICHA SECUENCIA DE CODIFICACION HETEROLOGA DESEADA; EN MANTENER DICHAS CELULAS DE MAMIFERO EN UN MEDIO EXENTO DE SUERO; Y EN INDUCIR DICHAS CELULAS CON UNA CANTIDAD EFICAZ DE UN MEDIADOR DE INDUCCION Y UNA CANTIDAD EFICAZ DE IONES METALICOS NO TOXICOS. COMO MEDIADOR DE INDUCCION SE UTILIZA TRANSFERRINA HUMANA EN CONCENTRACION SUPERIOR A 10 MG/ML.
ES551913A 1985-02-13 1986-02-12 Un metodo para obtener expresion de una secuencia de codificacion deseada en celulas de mamifero Expired ES8707564A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70129685A 1985-02-13 1985-02-13
US80167485A 1985-11-25 1985-11-25

Publications (2)

Publication Number Publication Date
ES551913A0 ES551913A0 (es) 1987-08-01
ES8707564A1 true ES8707564A1 (es) 1987-08-01

Family

ID=27106764

Family Applications (1)

Application Number Title Priority Date Filing Date
ES551913A Expired ES8707564A1 (es) 1985-02-13 1986-02-12 Un metodo para obtener expresion de una secuencia de codificacion deseada en celulas de mamifero

Country Status (9)

Country Link
US (1) US5089397A (es)
EP (1) EP0217822B1 (es)
AT (1) ATE89314T1 (es)
AU (1) AU580145B2 (es)
CA (1) CA1338829C (es)
DE (1) DE3688418T2 (es)
DK (1) DK488386A (es)
ES (1) ES8707564A1 (es)
WO (1) WO1986004920A1 (es)

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Also Published As

Publication number Publication date
AU5513586A (en) 1986-09-10
DE3688418T2 (de) 1993-08-26
EP0217822A1 (en) 1987-04-15
CA1338829C (en) 1997-01-07
EP0217822A4 (en) 1989-05-11
WO1986004920A1 (en) 1986-08-28
ES551913A0 (es) 1987-08-01
ATE89314T1 (de) 1993-05-15
AU580145B2 (en) 1989-01-05
DK488386D0 (da) 1986-10-13
DE3688418D1 (de) 1993-06-17
EP0217822B1 (en) 1993-05-12
DK488386A (da) 1986-12-15
US5089397A (en) 1992-02-18

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