ES8702491A1 - Un metodo para expresar el factor supresor de alergias (fsa) - Google Patents

Un metodo para expresar el factor supresor de alergias (fsa)

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Publication number
ES8702491A1
ES8702491A1 ES549057A ES549057A ES8702491A1 ES 8702491 A1 ES8702491 A1 ES 8702491A1 ES 549057 A ES549057 A ES 549057A ES 549057 A ES549057 A ES 549057A ES 8702491 A1 ES8702491 A1 ES 8702491A1
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ES
Spain
Prior art keywords
asf
allergy
human
cultivating
hat
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES549057A
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English (en)
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ES549057A0 (es
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Individual
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Individual
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Publication date
Application filed by Individual filed Critical Individual
Priority to ES549057A priority Critical patent/ES8702491A1/es
Publication of ES8702491A1 publication Critical patent/ES8702491A1/es
Publication of ES549057A0 publication Critical patent/ES549057A0/es
Expired legal-status Critical Current

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Abstract

METODO PARA EXPRESAR EL FACTOR SUPRESOR DE ALERGIAS (FSA). COMPRENDE: A) PREPARACION DE HIBRIDOMA DE CELULAS T HUMANAS CAPACES DE EXPRESAR EL FSA MEDIANTE LAS SIGUIENTES OPERACIONES: AISLAR LINFOCITOS DE SANGRE PERIFERICA HUMANA Y CULTIVARLOS DURANTE 7 DIAS CON MITOGENO DE HIERBA CARMIN, FUSIONANDOLOS DESPUES CON UN CLON DE LA LINEA JKOT CELULAR JURKAT RESISTENTE A TIOGUANINA Y OUABAINA Y SENSIBLE A HIPOXANTINA, AMINOPTERINA Y TIMIDINA (HAT), MEDIANTE POLIETILENGLICOL, CULTIVANDO EN PLACA LAS CELULAS DE ALIMENTACION; B) AISLAMIENTO DE LOS HIBRIDOMAS PREPARADOS EN A), MEDIANTE CULTIVO DE CLONES INDIVIDUALES EN PRESENCIA DE PENICILINA Y ESTREPTOMICINA COLOCANDO ESOS CULTIVOS EN UNA ATMOSFERA HUMANA CON UN 5% DE CO2 DURANTE UNAS 7 SEMANAS EN PRESENCIA O AUSENCIA DE HAT; Y C) RECOGIDA DEL FSA PRODUCIDO DEL FLUIDO SOBRENADANTE DEL CULTIVO DE CELULAS CONSUMIDO DIRECTAMENTE DE LOS CLONES DE HIBRIDOMA EN EL MEDIO.
ES549057A 1985-11-19 1985-11-19 Un metodo para expresar el factor supresor de alergias (fsa) Expired ES8702491A1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES549057A ES8702491A1 (es) 1985-11-19 1985-11-19 Un metodo para expresar el factor supresor de alergias (fsa)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES549057A ES8702491A1 (es) 1985-11-19 1985-11-19 Un metodo para expresar el factor supresor de alergias (fsa)

Publications (2)

Publication Number Publication Date
ES8702491A1 true ES8702491A1 (es) 1986-12-16
ES549057A0 ES549057A0 (es) 1986-12-16

Family

ID=8490220

Family Applications (1)

Application Number Title Priority Date Filing Date
ES549057A Expired ES8702491A1 (es) 1985-11-19 1985-11-19 Un metodo para expresar el factor supresor de alergias (fsa)

Country Status (1)

Country Link
ES (1) ES8702491A1 (es)

Also Published As

Publication number Publication date
ES549057A0 (es) 1986-12-16

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