ES8501439A1 - Secuencia de adn y estructuras de adn y procedimiento que las utiliza para la preparacion de penicilin-acilasa - Google Patents

Secuencia de adn y estructuras de adn y procedimiento que las utiliza para la preparacion de penicilin-acilasa

Info

Publication number
ES8501439A1
ES8501439A1 ES526659A ES526659A ES8501439A1 ES 8501439 A1 ES8501439 A1 ES 8501439A1 ES 526659 A ES526659 A ES 526659A ES 526659 A ES526659 A ES 526659A ES 8501439 A1 ES8501439 A1 ES 8501439A1
Authority
ES
Spain
Prior art keywords
dna
preparation
penicillin acylase
structures
dna sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES526659A
Other languages
English (en)
Other versions
ES526659A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GESELLSCHAFT fur BIOTECHNOLOGISCHE FORSCHUNG MGH
Original Assignee
GESELLSCHAFT fur BIOTECHNOLOGISCHE FORSCHUNG MGH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GESELLSCHAFT fur BIOTECHNOLOGISCHE FORSCHUNG MGH filed Critical GESELLSCHAFT fur BIOTECHNOLOGISCHE FORSCHUNG MGH
Publication of ES8501439A1 publication Critical patent/ES8501439A1/es
Publication of ES526659A0 publication Critical patent/ES526659A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROCEDIMIENTO PARA LA PREPARACION DE PENICILIN-ACILASA.COMPRENDE LAS SIGUIENTES OPERACIONES: PRIMERA, SE PREPARARA UNA SUBSECUENCIA DE ADN QUE CODIFICA UNO DE LOS COMPONENTES DE LA PENICILIN-ACILASA EN FORMA DE UNA SUBUNIDAD DE APROXIMADAMENTE 20 KD, CON LA SIGUIENTE SECUENCIA DE PROTEINA AMINO-TERMINAL: H2N-GLU-GLN-SER-SER-SER-GLU-LLE-LYS-LLE-VAL-ARG-ASP- ETC.; SEGUNDA, SE PREPARA UNA SUBSECUENCIA DE ADN QUE CODIFICA EL OTRO COMPONENTE DE LA PENICILIN-ACILASA EN FORMA DE UNA SUBUNIDAD DE APROXIMIDAMENTE 65 KD; Y POR ULTIMO, SE INTERCAMBIA EL PROMOTOR O EL PROMOTOR-DIRECTOR, SEGUN UN ESQUEMA PREDETERMINADO.
ES526659A 1982-10-21 1983-10-21 Secuencia de adn y estructuras de adn y procedimiento que las utiliza para la preparacion de penicilin-acilasa Granted ES526659A0 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3238976 1982-10-21
DE19833330003 DE3330003A1 (de) 1982-10-21 1983-08-19 Dna-sequenz und dna-strukturen und sie verwendendes verfahren zur herstellung von penicillin-acylase

Publications (2)

Publication Number Publication Date
ES8501439A1 true ES8501439A1 (es) 1984-12-01
ES526659A0 ES526659A0 (es) 1984-12-01

Family

ID=25805251

Family Applications (1)

Application Number Title Priority Date Filing Date
ES526659A Granted ES526659A0 (es) 1982-10-21 1983-10-21 Secuencia de adn y estructuras de adn y procedimiento que las utiliza para la preparacion de penicilin-acilasa

Country Status (4)

Country Link
EP (1) EP0107823A3 (es)
DE (1) DE3330003A1 (es)
DK (1) DK482583A (es)
ES (1) ES526659A0 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554250A (en) * 1983-06-30 1985-11-19 E. R. Squibb & Sons, Inc. Method of increased production of penicillin acylase and plasmid employed therein
SE8403929L (sv) * 1984-07-31 1986-02-21 Sten Gatenbeck Rekombinant-dna-molekyl, transformerade mikroorganismer och forfarande for framstellning av penicillin v-amidas
DE3439843A1 (de) * 1984-10-31 1986-04-30 Boehringer Mannheim Gmbh, 6800 Mannheim Plasmide zur erhoehten produktion von penicillin g-amidase
FR2579224B1 (fr) * 1985-03-25 1987-05-22 Genetica Procede de preparation microbiologique de la serum-albumine humaine
IL78531A0 (en) * 1985-04-22 1986-08-31 Lilly Co Eli Recombinant dna expression vectors and dna compounds which encode isopenicillin n synthetase
JPH04228073A (ja) * 1990-04-18 1992-08-18 Gist Brocades Nv ペニシリンｇアシラーゼ、それをコードする遺伝子及び該酵素の製造方法
AU4436900A (en) * 1999-04-29 2000-11-17 Dsm N.V. Expression cassette for efficient production of a protein

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2930794C2 (de) * 1979-07-28 1983-02-10 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Herstellung von Mikroorganismen zur Spaltung oder Synthese von β-Lactamantibiotika
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer

Also Published As

Publication number Publication date
DK482583D0 (da) 1983-10-20
DK482583A (da) 1984-04-22
EP0107823A2 (de) 1984-05-09
ES526659A0 (es) 1984-12-01
EP0107823A3 (de) 1985-03-13
DE3330003A1 (de) 1984-10-31

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