ES2959127T3 - Secuenciación de ácidos nucleicos de alto rendimiento por expansión - Google Patents

Secuenciación de ácidos nucleicos de alto rendimiento por expansión Download PDF

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Publication number
ES2959127T3
ES2959127T3 ES15164186T ES15164186T ES2959127T3 ES 2959127 T3 ES2959127 T3 ES 2959127T3 ES 15164186 T ES15164186 T ES 15164186T ES 15164186 T ES15164186 T ES 15164186T ES 2959127 T3 ES2959127 T3 ES 2959127T3
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tether
xpandomer
probe
template
substrate
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Mark Stamatios Kokoris
Robert N Mcruer
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F Hoffmann La Roche AG
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES15164186T 2007-06-19 2008-06-19 Secuenciación de ácidos nucleicos de alto rendimiento por expansión Active ES2959127T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US94503107P 2007-06-19 2007-06-19
US98191607P 2007-10-23 2007-10-23
US30507P 2007-10-25 2007-10-25

Publications (1)

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ES2959127T3 true ES2959127T3 (es) 2024-02-20

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ES15164186T Active ES2959127T3 (es) 2007-06-19 2008-06-19 Secuenciación de ácidos nucleicos de alto rendimiento por expansión
ES08771483.8T Active ES2559313T3 (es) 2007-06-19 2008-06-19 Secuenciación de ácidos nucleicos de alto rendimiento por expansión

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US (8) US7939259B2 (show.php)
EP (2) EP2171088B1 (show.php)
JP (1) JP5745842B2 (show.php)
KR (1) KR101685208B1 (show.php)
CN (1) CN102083998B (show.php)
AU (1) AU2008265691B2 (show.php)
CA (1) CA2691364C (show.php)
DK (1) DK2171088T3 (show.php)
ES (2) ES2959127T3 (show.php)
HK (1) HK1217736A1 (show.php)
IL (2) IL202821A (show.php)
WO (1) WO2008157696A2 (show.php)

Families Citing this family (203)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060030036A1 (en) 2004-05-28 2006-02-09 Victor Joseph Chips for multiplex analyses
WO2007041621A2 (en) * 2005-10-03 2007-04-12 Xingsheng Sean Ling Hybridization assisted nanopore sequencing
PL1979407T3 (pl) * 2006-01-19 2015-03-31 Allexcel Inc Solubizacja i ukierunkowane dostarczanie leków samoorganizującymi się polimerami amfifilowymi
WO2007146158A1 (en) * 2006-06-07 2007-12-21 The Trustees Of Columbia University In The City Of New York Dna sequencing by nanopore using modified nucleotides
EP3543357A1 (en) 2007-05-08 2019-09-25 Trustees of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
EP2171088B1 (en) * 2007-06-19 2015-10-14 Stratos Genomics Inc. High throughput nucleic acid sequencing by expansion
EP2201136B1 (en) * 2007-10-01 2017-12-06 Nabsys 2.0 LLC Nanopore sequencing by hybridization of probes to form ternary complexes and variable range alignment
US8592182B2 (en) * 2007-10-23 2013-11-26 Stratos Genomics Inc. High throughput nucleic acid sequencing by spacing
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
EP2342362B1 (en) * 2008-09-03 2017-03-01 Nabsys 2.0 LLC Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
CN102365367A (zh) * 2009-01-29 2012-02-29 斯特拉托斯基因公司 通过展开进行高通量核酸测序和相关方法
US8455260B2 (en) * 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
EP2411536B1 (en) * 2009-03-27 2014-09-17 Nabsys, Inc. Methods for analyzing biomolecules and probes bound thereto
WO2010114842A1 (en) 2009-03-30 2010-10-07 Ibis Biosciences, Inc. Bioagent detection systems, devices, and methods
EP2425240A4 (en) 2009-04-30 2012-12-12 Good Start Genetics Inc METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
US8246799B2 (en) * 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
AU2010301128B2 (en) 2009-09-30 2014-09-18 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US8586301B2 (en) 2010-06-30 2013-11-19 Stratos Genomics, Inc. Multiplexed identification of nucleic acid sequences
US9309556B2 (en) 2010-09-24 2016-04-12 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
WO2012058647A1 (en) * 2010-10-28 2012-05-03 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
US8865404B2 (en) * 2010-11-05 2014-10-21 President And Fellows Of Harvard College Methods for sequencing nucleic acid molecules
WO2012067911A1 (en) 2010-11-16 2012-05-24 Nabsys, Inc. Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
US8805095B2 (en) 2010-12-03 2014-08-12 International Business Machines Corporation Analysing character strings
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
CN103502463B (zh) 2010-12-27 2016-03-16 艾比斯生物科学公司 核酸样品的制备方法及组合物
US9581563B2 (en) 2011-01-24 2017-02-28 Genia Technologies, Inc. System for communicating information from an array of sensors
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
WO2012109574A2 (en) * 2011-02-11 2012-08-16 Nabsys, Inc. Assay methods using dna binding proteins
WO2013022778A1 (en) 2011-08-05 2013-02-14 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
CN104066850B (zh) 2011-09-23 2017-11-10 牛津楠路珀尔科技有限公司 包含聚合物单元的聚合物的分析
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
WO2013096838A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids
ES2952728T3 (es) 2011-12-22 2023-11-03 Harvard College Métodos para la detección de analitos
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
WO2013096819A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
WO2013096799A1 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
WO2013101935A1 (en) 2011-12-27 2013-07-04 Ibis Biosciences, Inc. Bioagent detection oligonucleotides
WO2013102091A1 (en) 2011-12-28 2013-07-04 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
WO2013102081A2 (en) 2011-12-29 2013-07-04 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
WO2013101741A1 (en) 2011-12-30 2013-07-04 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
JP2015503923A (ja) 2012-01-09 2015-02-05 オスロ ウニヴェルスィテーツスィーケフース ハーエフOslo Universitetssykehus Hf 結腸直腸癌の解析のための方法およびバイオマーカー
EP3434789A1 (en) 2012-01-13 2019-01-30 Data2Bio Genotyping by next-generation sequencing
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
EP2834370B1 (en) 2012-04-03 2019-01-02 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and crohn's disease
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
WO2013166303A1 (en) 2012-05-02 2013-11-07 Ibis Biosciences, Inc. Dna sequencing
US20150133310A1 (en) 2012-05-02 2015-05-14 Ibis Biosciences, Inc. Nucleic acid sequencing systems and methods
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
CA2876095A1 (en) 2012-06-15 2013-12-19 Genia Technologies, Inc. Chip set-up and high-accuracy nucleic acid sequencing
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
WO2014014991A2 (en) 2012-07-19 2014-01-23 President And Fellows Of Harvard College Methods of storing information using nucleic acids
WO2014062835A1 (en) 2012-10-16 2014-04-24 Abbott Molecular Inc. Methods and apparatus to sequence a nucleic acid
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
US9605309B2 (en) * 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
WO2014074922A1 (en) 2012-11-09 2014-05-15 Stratos Genomics, Inc. Concentrating a target molecule for sensing by a nanopore
GB201222928D0 (en) * 2012-12-19 2013-01-30 Oxford Nanopore Tech Ltd Analysis of a polynucleotide
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
EP2956550B1 (en) 2013-01-18 2020-04-08 Nabsys 2.0 LLC Enhanced probe binding
WO2014112950A1 (en) * 2013-01-18 2014-07-24 Nanyang Technological University Method of preparing a keratin-based biomaterial and keratin-based biomaterial formed thereof
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US10138509B2 (en) 2013-03-12 2018-11-27 President And Fellows Of Harvard College Method for generating a three-dimensional nucleic acid containing matrix
EP2971154A4 (en) 2013-03-14 2017-03-01 Ibis Biosciences, Inc. Nucleic acid control panels
JP2016512437A (ja) 2013-03-14 2016-04-28 アボツト・モレキユラー・インコーポレイテツド 多重メチル化特異的増幅システムおよび方法
CA2905410A1 (en) 2013-03-15 2014-09-25 Abbott Molecular Inc. Systems and methods for detection of genomic copy number changes
EP2971140B1 (en) 2013-03-15 2019-01-16 Ibis Biosciences, Inc. Methods to assess contamination in dna sequencing
WO2014190322A2 (en) * 2013-05-24 2014-11-27 Quantapore, Inc. Nanopore-based nucleic acid analysis with mixed fret detection
CA3176690A1 (en) 2013-06-04 2014-12-11 President And Fellows Of Harvard College Rna-guided transcriptional regulation
US20150037787A1 (en) * 2013-07-31 2015-02-05 International Business Machines Corporation Polynucleotide configuration for reliable electrical and optical sensing
WO2015026853A2 (en) 2013-08-19 2015-02-26 Abbott Molecular Inc. Next-generation sequencing libraries
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US10174381B2 (en) 2013-10-18 2019-01-08 The Regents Of The University Of Michigan Systems and methods for determining a treatment course of action
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
CA2926138A1 (en) 2013-10-23 2015-04-30 Genia Technologies, Inc. High speed molecular sensing with nanopores
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
WO2015095355A2 (en) 2013-12-17 2015-06-25 The Brigham And Women's Hospital, Inc. Detection of an antibody against a pathogen
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
JP2017514467A (ja) * 2014-04-23 2017-06-08 チルドレンズ メディカル センター コーポレーション 核酸キャリパーを用いた高スループット構造決定
US10934581B2 (en) 2014-04-30 2021-03-02 International Business Machines Corporation Bow tie DNA compositions and methods
US20150315637A1 (en) * 2014-04-30 2015-11-05 International Business Machines Corporation Bow tie dna compositions and methods
EP3143158A1 (en) 2014-05-14 2017-03-22 Stratos Genomics, Inc. Translocation control for sensing by a nanopore
EP3151733B1 (en) 2014-06-06 2020-04-15 The Regents Of The University Of Michigan Compositions and methods for characterizing and diagnosing periodontal disease
US11155809B2 (en) 2014-06-24 2021-10-26 Bio-Rad Laboratories, Inc. Digital PCR barcoding
JP2017521654A (ja) 2014-06-27 2017-08-03 アボット・ラボラトリーズAbbott Laboratories ヒトペギウイルス2(HPgV−2)を検出するための組成物および方法
WO2016007839A1 (en) 2014-07-11 2016-01-14 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
EP3835430B1 (en) 2014-07-24 2023-08-16 Abbott Molecular Inc. Compositions and methods for the detection and analysis of mycobacterium tuberculosis
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
AU2015319825A1 (en) * 2014-09-26 2017-04-27 Nooma Bio, Inc. Target sequence detection by nanopore sensing of synthetic probes
WO2016053881A1 (en) * 2014-10-03 2016-04-07 Life Technologies Corporation Genetic sequence verification compositions, methods and kits
AU2015330841B2 (en) 2014-10-10 2019-08-15 Quantapore, Inc. Nanopore-based polymer analysis with mutually-quenching fluorescent labels
KR102551897B1 (ko) 2014-10-16 2023-07-06 옥스포드 나노포어 테크놀로지즈 피엘씨 폴리머의 분석
EP3209800B1 (en) 2014-10-24 2022-02-16 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
AU2015349730C1 (en) 2014-11-20 2020-04-30 F. Hoffmann-La Roche Ag Nulceoside phosphoroamidate esters and derivatives thereof, use and synthesis thereof
CN107077533B (zh) * 2014-12-10 2021-07-27 深圳华大生命科学研究院 测序数据处理装置和方法
CN107077538B (zh) * 2014-12-10 2020-08-07 深圳华大生命科学研究院 测序数据处理装置和方法
CN107075564A (zh) * 2014-12-10 2017-08-18 深圳华大基因研究院 确定肿瘤核酸浓度的方法和装置
US10066259B2 (en) * 2015-01-06 2018-09-04 Good Start Genetics, Inc. Screening for structural variants
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
US10641772B2 (en) 2015-02-20 2020-05-05 Takara Bio Usa, Inc. Method for rapid accurate dispensing, visualization and analysis of single cells
EP3322812B1 (en) 2015-07-13 2022-05-18 President and Fellows of Harvard College Methods for retrievable information storage using nucleic acids
EP3322483A4 (en) 2015-07-14 2019-01-02 Abbott Molecular Inc. Compositions and methods for identifying drug resistant tuberculosis
WO2017011538A1 (en) 2015-07-14 2017-01-19 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides
HUE061388T2 (hu) 2015-07-30 2023-06-28 Massachusetts Eye & Ear Infirmary Õsi vírusszekvenciák és alkalmazásaik
MX2018005611A (es) 2015-11-03 2018-11-09 Harvard College Metodo y aparato para la formacion de imagenes volumetricas de una matriz tridimensional que contiene acido nucleico.
CA3004310A1 (en) * 2015-11-04 2017-05-11 Atreca, Inc. Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells
CA3004883A1 (en) 2015-11-16 2017-05-26 Stratos Genomics, Inc. Dp04 polymerase variants
EP3400298B1 (en) 2016-01-08 2024-03-06 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
AU2017218431B2 (en) 2016-02-08 2022-08-04 RGENE, Inc. Multiple ligase compositions, systems, and methods
EP3414347A4 (en) * 2016-02-11 2019-10-09 Qiagen Sciences, LLC ADDITIVE FOR IMPROVING SEQUENCING THROUGH SYNTHETIC PERFORMANCE
EP3430154B1 (en) 2016-03-14 2020-11-11 Rgene, Inc. Hyper-thermostable lysine-mutant ssdna/rna ligases
US11486873B2 (en) 2016-03-31 2022-11-01 Ontera Inc. Multipore determination of fractional abundance of polynucleotide sequences in a sample
CN116200465A (zh) 2016-04-25 2023-06-02 哈佛学院董事及会员团体 用于原位分子检测的杂交链反应方法
EP3482196B1 (en) 2016-07-05 2022-02-23 Quantapore, Inc. Optically based nanopore sequencing
WO2018013509A1 (en) 2016-07-11 2018-01-18 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
US10781175B2 (en) 2016-07-15 2020-09-22 Am Chemicals Llc Solid supports and phosphoramidite building blocks for oligonucleotide conjugates
US11460405B2 (en) 2016-07-21 2022-10-04 Takara Bio Usa, Inc. Multi-Z imaging and dispensing with multi-well devices
WO2018042251A1 (en) 2016-08-29 2018-03-08 Oslo Universitetssykehus Hf Chip-seq assays
WO2018045181A1 (en) 2016-08-31 2018-03-08 President And Fellows Of Harvard College Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing
CN109923216B (zh) 2016-08-31 2024-08-02 哈佛学院董事及会员团体 将生物分子的检测组合到使用荧光原位测序的单个试验的方法
US12116570B2 (en) 2016-09-29 2024-10-15 Roche Sequencing Solutions, Inc. Directed evolution of enzymes by plasmid tagging in droplets
WO2018069484A2 (en) 2016-10-13 2018-04-19 F. Hoffmann-La Roche Ag Molecular detection and counting using nanopores
EP3555290B1 (en) 2016-12-19 2022-11-02 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
ES2987024T3 (es) 2017-05-04 2024-11-13 Hoffmann La Roche Variantes de polimerasa DP04
CA3066645A1 (en) 2017-06-08 2018-12-13 The Brigham And Women's Hospital, Inc Methods and compositions for identifying epitopes
EP3642363B1 (en) 2017-06-20 2025-12-24 Bio-Rad Laboratories, Inc. Mda using bead oligonucleotide
WO2019030149A1 (en) 2017-08-07 2019-02-14 Dna Script VARIANTS OF FAMILY A DNA POLYMERASE AND USES THEREOF
EP3704247B1 (en) 2017-11-02 2023-01-04 Bio-Rad Laboratories, Inc. Transposase-based genomic analysis
US11131646B2 (en) 2017-11-03 2021-09-28 Robert Bosch Gmbh Electrochemical sequencing of DNA using an edge electrode
CA3084812A1 (en) 2017-12-11 2019-06-20 Stratos Genomics, Inc. Dpo4 polymerase variants with improved accuracy
CN111836904A (zh) * 2017-12-21 2020-10-27 豪夫迈·罗氏有限公司 用于单向核酸测序的组合物和方法
EP3735477B1 (en) 2018-01-05 2024-10-30 Roche Diagnostics GmbH Enhancement of nucleic acid polymerization by minor groove binding moieties
US11970731B2 (en) 2018-01-05 2024-04-30 Roche Sequencing Solutions, Inc. Enhancement of nucleic acid polymerization by aromatic compounds
CA3087714A1 (en) 2018-01-08 2019-07-11 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
JP6559814B2 (ja) * 2018-01-26 2019-08-14 株式会社日立ハイテクノロジーズ 生体分子測定方法
US12209277B2 (en) * 2018-01-31 2025-01-28 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
US12037707B2 (en) 2018-04-05 2024-07-16 Massachusetts Eye And Ear Infirmary Methods of making and using combinatorial barcoded nucleic acid libraries having defined variation
US11512002B2 (en) 2018-04-18 2022-11-29 University Of Virginia Patent Foundation Silica materials and methods of making thereof
US12235273B2 (en) 2018-05-04 2025-02-25 Abbott Laboratories HBV diagnostic, prognostic, and therapeutic methods and products
WO2020020608A1 (en) 2018-07-23 2020-01-30 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
CN112770776B (zh) 2018-07-30 2025-08-19 瑞德库尔有限责任公司 用于样品处理或分析的方法和系统
US11479816B2 (en) 2018-08-20 2022-10-25 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
WO2020102513A1 (en) 2018-11-14 2020-05-22 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for characterizing and treating cancer
AU2019398307A1 (en) 2018-12-13 2021-06-10 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
AU2020223456A1 (en) 2019-02-12 2021-08-12 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.
CA3131115A1 (en) * 2019-02-21 2020-08-27 Stratos Genomics, Inc. Methods, compositions, and devices for solid-state synthesis of expandable polymers for use in single molecule sequencing
EP3972976A4 (en) * 2019-05-23 2023-07-19 Stratos Genomics Inc. TRANSLOCATION CONTROL ELEMENTS, REPORTER CODES AND OTHER TRANSLOCATION CONTROL MEANS FOR USE IN NANOPOORE SEQUENCING
WO2020254672A1 (en) 2019-06-19 2020-12-24 Therycell Gmbh Spatial characterisation of target structures in a sample
US11755922B2 (en) * 2019-10-04 2023-09-12 The Board Of Trustees Of The University Of Illinois On-chip nanoscale storage system using chimeric DNA
WO2021087118A1 (en) 2019-10-30 2021-05-06 Stratos Genomics, Inc. Methods and compositions for assembly of biological nanopores
JP7344786B2 (ja) * 2019-12-19 2023-09-14 株式会社日立製作所 溶液中の任意のdna配列を同定する方法
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
US20230203592A1 (en) 2020-05-05 2023-06-29 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
WO2021249985A1 (en) 2020-06-10 2021-12-16 F. Hoffmann-La Roche Ag Faradaic systems and methods for self-limiting protein pore insertion in a membrane
EP4185301A4 (en) 2020-07-24 2025-05-07 The Regents Of The University Of Michigan Compositions and methods for detecting and treating high grade subtypes of uterine cancer
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants
CN112201305B (zh) * 2020-10-12 2021-12-07 冬青南京生物科技有限公司 一种单细胞核中基因数目测定设备
GB202103605D0 (en) 2021-03-16 2021-04-28 Oxford Nanopore Tech Ltd Alignment of target and reference sequences of polymer units
US20240377380A1 (en) 2021-03-31 2024-11-14 Illumina, Inc. Nanopore sensor devices
US20230014008A1 (en) * 2021-07-13 2023-01-19 10X Genomics, Inc. Methods for improving spatial performance
JP7787988B2 (ja) 2021-10-04 2025-12-17 エフ. ホフマン-ラ ロシュ アーゲー オンラインベースコール圧縮
US20240361297A1 (en) 2021-11-08 2024-10-31 Illumina, Inc. Identifying nucleotides using changes in impedance between electrodes
CA3253952A1 (en) 2022-03-28 2023-10-05 F. Hoffmann-La Roche Ag SYNTHESIS OF PHOSPHORAMIDITES BASED ON ISOMERICALLY PURE POLYOLS
US20250223637A1 (en) 2022-03-31 2025-07-10 Illumina, Inc. Devices including osmotically balanced barriers, and methods of making and using the same
WO2023187112A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Amphiphilic block-co-polymers cross-linked with different cross-linkers consecutively forming a cross-linking hierarchy
US20230312856A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Nanopore devices including barriers using diblock or triblock copolymers, and methods of making the same
WO2023187081A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Methods for inserting nanopores into polymeric membranes using chaotropic solvents
US20240076322A1 (en) 2022-03-31 2024-03-07 Illumina Cambridge Limited Barriers including cross-linked amphiphilic molecules, and methods of making the same
US20250205655A1 (en) 2022-03-31 2025-06-26 Illumina, Inc. Amphiphilic polymers to be used in barriers and preparation thereof, barriers with nanopores and preparation thereof
JP2025517595A (ja) 2022-03-31 2025-06-10 イルミナ インコーポレイテッド Dna配列決定のための生物学的ナノポアを含むバリアであって、末端基及び/又は中間基を有するコポリマーから作製されるバリア、並びにその作製方法
AU2023264238A1 (en) * 2022-05-02 2024-10-10 F. Hoffmann-La Roche Ag Compositions and methods that reduce prussian blue formation during nanopore sequencing
EP4599083A1 (en) 2022-10-03 2025-08-13 F. Hoffmann-La Roche AG Single molecule multi-molecular trace methods and systems
EP4605545A1 (en) 2022-10-21 2025-08-27 F. Hoffmann-La Roche AG Detection of modified nucleobases in nucleic acid samples
AU2024207414A1 (en) 2023-01-13 2025-06-12 F. Hoffmann-La Roche Ag Detection of modified nucleobases in dna samples
AU2024246785A1 (en) 2023-03-30 2025-09-11 F. Hoffmann-La Roche Ag Modulation of target molecule-lipid bilayer interactions
CN121240921A (zh) 2023-05-04 2025-12-30 豪夫迈·罗氏有限公司 用于纳米孔测序的混合三嵌段共聚物膜组合物和方法
CN121057766A (zh) 2023-05-04 2025-12-02 豪夫迈·罗氏有限公司 经修饰的三嵌段共聚物化合物及其使用方法
WO2024242847A1 (en) 2023-05-25 2024-11-28 Illumina, Inc. Methods of making barriers including nanopores and cross¬ linked amphiphilic molecules, and barriers formed using same
WO2025082960A1 (en) 2023-10-18 2025-04-24 F. Hoffmann-La Roche Ag Dpo4 polymerase variants with improved properties
US20250161882A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Fluidic devices, nanopore instruments, and methods
WO2025137293A2 (en) 2023-12-22 2025-06-26 Roche Sequencing Solutions, Inc. Enhancement of nucleic acid polymerization by aromatic compounds
WO2025132780A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025132779A2 (en) 2023-12-22 2025-06-26 F. Hoffmann-La Roche Ag Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion
WO2025149478A1 (en) 2024-01-12 2025-07-17 F. Hoffmann-La Roche Ag Compositions of modified nucleoside triphosphates
WO2025149479A1 (en) 2024-01-12 2025-07-17 Roche Diagnostics Gmbh Synthesis of modified nucleoside triphosphates
WO2025174660A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Recharging for nanopore system
WO2025174659A1 (en) 2024-02-12 2025-08-21 Illumina, Inc. Net-zero current scheme for nanopore system
EP4603180A1 (en) 2024-02-15 2025-08-20 Roche Diagnostics International AG Techniques for synthesizing a macromolecule from a sample
WO2025212586A1 (en) * 2024-04-02 2025-10-09 Roche Sequencing Solutions, Inc. High throughput inramolecular consensus reads
WO2025240460A2 (en) 2024-05-14 2025-11-20 Roche Molecular Systems, Inc. Assay for detection of mutations conferring resistance to treatment with an immunotherapeutic agent
CN118904278A (zh) * 2024-10-08 2024-11-08 祥符实验室 一种核酸固相合成提取磁珠及其制备方法和应用

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4415732A (en) 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
US5378825A (en) 1990-07-27 1995-01-03 Isis Pharmaceuticals, Inc. Backbone modified oligonucleotide analogs
US5614617A (en) 1990-07-27 1997-03-25 Isis Pharmaceuticals, Inc. Nuclease resistant, pyrimidine modified oligonucleotides that detect and modulate gene expression
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5432272A (en) 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
JP3739785B2 (ja) 1991-11-26 2006-01-25 アイシス ファーマシューティカルズ,インコーポレイティド 修飾されたピリミジンを含有するオリゴマーを使用する増強された三重らせんおよび二重らせんの成形
FR2702874B1 (fr) 1993-03-18 1995-06-09 Centre Nat Rech Scient Cellule memoire insensible aux rayonnements.
WO1994022892A1 (en) 1993-03-30 1994-10-13 Sterling Winthrop Inc. 7-deazapurine modified oligonucleotides
AU6632094A (en) 1993-04-19 1994-11-08 Gilead Sciences, Inc. Enhanced triple-helix and double-helix formation with oligomers containing modified purines
FR2705099B1 (fr) 1993-05-12 1995-08-04 Centre Nat Rech Scient Oligonucléotides phosphorothioates triesters et procédé de préparation.
DE4438918A1 (de) 1994-11-04 1996-05-09 Hoechst Ag Modifizierte Oligonukleotide, deren Herstellung sowie deren Verwendung
US6150510A (en) 1995-11-06 2000-11-21 Aventis Pharma Deutschland Gmbh Modified oligonucleotides, their preparation and their use
WO1998023733A2 (en) 1996-11-27 1998-06-04 University Of Washington Thermostable polymerases having altered fidelity
US7399844B2 (en) * 1998-07-09 2008-07-15 Agilent Technologies, Inc. Method and reagents for analyzing the nucleotide sequence of nucleic acids
US6242589B1 (en) 1998-07-14 2001-06-05 Isis Pharmaceuticals, Inc. Phosphorothioate oligonucleotides having modified internucleoside linkages
WO2000034527A2 (en) 1998-12-11 2000-06-15 The Regents Of The University Of California Targeted molecular bar codes
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
DE60041642D1 (de) 1999-03-18 2009-04-09 Complete Genomics As Methoden zur klonierung und herstelung von kettenfragmenten mit lesbarem inhalt an information
AU5631200A (en) * 1999-06-22 2001-01-09 President And Fellows Of Harvard College Control of solid state dimensional features
EP1072679A3 (en) 1999-07-20 2002-07-31 Agilent Technologies, Inc. (a Delaware corporation) Method of producing nucleic acid molecules with reduced secondary structure
MXPA02002163A (es) 1999-09-01 2004-04-21 Yeda Res & Dev Polimerizacion de acido nucleico dependiente de molde usando bloques de construccion de oligonucleotidos trifosfato.
US6329178B1 (en) 2000-01-14 2001-12-11 University Of Washington DNA polymerase mutant having one or more mutations in the active site
US6951720B2 (en) 2001-05-10 2005-10-04 San Diego State University Foundation Use of phosphorothiolate polynucleotides in ligating nucleic acids
GB0308852D0 (en) 2003-04-16 2003-05-21 Lingvitae As Method
US20070048748A1 (en) 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
WO2006076650A2 (en) * 2005-01-12 2006-07-20 Applera Corporation Compositions, methods, and kits for selective amplification of nucleic acids
WO2006084132A2 (en) * 2005-02-01 2006-08-10 Agencourt Bioscience Corp. Reagents, methods, and libraries for bead-based squencing
CN101189345A (zh) * 2005-02-01 2008-05-28 Ab先进基因分析公司 珠基测序的试剂、方法和文库
EP2171088B1 (en) * 2007-06-19 2015-10-14 Stratos Genomics Inc. High throughput nucleic acid sequencing by expansion
CN201189345Y (zh) * 2008-05-23 2009-02-04 北京航天长峰股份有限公司 一种氧浓度调节装置

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