ES2942546T3 - Métodos de alta sensibilidad para la cuantificación paralela precisa de ácidos nucleicos - Google Patents
Métodos de alta sensibilidad para la cuantificación paralela precisa de ácidos nucleicos Download PDFInfo
- Publication number
- ES2942546T3 ES2942546T3 ES21163300T ES21163300T ES2942546T3 ES 2942546 T3 ES2942546 T3 ES 2942546T3 ES 21163300 T ES21163300 T ES 21163300T ES 21163300 T ES21163300 T ES 21163300T ES 2942546 T3 ES2942546 T3 ES 2942546T3
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- Prior art keywords
- probe
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- 238000000034 method Methods 0.000 title claims abstract 22
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract 6
- 108020004707 nucleic acids Proteins 0.000 title claims abstract 5
- 102000039446 nucleic acids Human genes 0.000 title claims abstract 5
- 238000011002 quantification Methods 0.000 title 1
- 239000000523 sample Substances 0.000 claims abstract 54
- 230000002068 genetic effect Effects 0.000 claims abstract 3
- 102000004533 Endonucleases Human genes 0.000 claims 6
- 108010042407 Endonucleases Proteins 0.000 claims 6
- 108091034117 Oligonucleotide Proteins 0.000 claims 5
- 238000009396 hybridization Methods 0.000 claims 5
- 239000007787 solid Substances 0.000 claims 4
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical group N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 108700028369 Alleles Proteins 0.000 claims 1
- 108090001008 Avidin Proteins 0.000 claims 1
- 108010090804 Streptavidin Proteins 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 239000008280 blood Substances 0.000 claims 1
- 210000004369 blood Anatomy 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000006073 displacement reaction Methods 0.000 claims 1
- 238000012165 high-throughput sequencing Methods 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 claims 1
- 102000054765 polymorphisms of proteins Human genes 0.000 claims 1
- 238000011176 pooling Methods 0.000 claims 1
- 238000005096 rolling process Methods 0.000 claims 1
- 210000003296 saliva Anatomy 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 1
- 210000002700 urine Anatomy 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 abstract 1
- 238000007481 next generation sequencing Methods 0.000 abstract 1
- 239000002853 nucleic acid probe Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
- C12Q2535/122—Massive parallel sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2545/00—Reactions characterised by their quantitative nature
- C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP21163300.3A EP4060050B1 (en) | 2021-03-18 | 2021-03-18 | Highly sensitive methods for accurate parallel quantification of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2942546T3 true ES2942546T3 (es) | 2023-06-02 |
Family
ID=75108125
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES21163300T Active ES2942546T3 (es) | 2021-03-18 | 2021-03-18 | Métodos de alta sensibilidad para la cuantificación paralela precisa de ácidos nucleicos |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US11486003B2 (https=) |
| EP (2) | EP4060050B1 (https=) |
| JP (2) | JP7651497B2 (https=) |
| KR (1) | KR20220130592A (https=) |
| CN (1) | CN115109842A (https=) |
| CA (1) | CA3149025A1 (https=) |
| DK (1) | DK4060050T3 (https=) |
| ES (1) | ES2942546T3 (https=) |
| FI (1) | FI4060050T3 (https=) |
| PT (1) | PT4060050T (https=) |
| TW (1) | TWI864378B (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US12435364B2 (en) * | 2021-08-16 | 2025-10-07 | 10X Genomics, Inc. | Probes comprising a split barcode region and methods of use |
| US20240068010A1 (en) * | 2022-08-31 | 2024-02-29 | Genomill Health Oy | Highly sensitive methods for accurate parallel quantification of variant nucleic acids |
| CN117512070B (zh) * | 2022-10-14 | 2024-08-16 | 深圳赛陆医疗科技有限公司 | 在载体上生成探针的方法和样品中靶物质的检测方法 |
| US20250146063A1 (en) * | 2023-11-02 | 2025-05-08 | Genomill Health Oy | Method for high-throughput detection of target nucleotide sequences |
| EP4610367B1 (en) * | 2024-03-01 | 2026-02-04 | Genomill Health Oy | Methods for accurate parallel amplification, detection and quantification of nucleic acids |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1985714B1 (en) * | 1998-03-25 | 2012-02-29 | Olink AB | Method and kit for detecting a target molecule employing at least two affinity probes and rolling circle replication of padlock probes |
| US9856521B2 (en) * | 2015-01-27 | 2018-01-02 | BioSpyder Technologies, Inc. | Ligation assays in liquid phase |
| GB2570412A (en) * | 2016-08-31 | 2019-07-24 | Harvard College | Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing |
| GB201621514D0 (en) * | 2016-12-16 | 2017-02-01 | Q-Linea Ab | Padlock probe detection method |
| WO2018175779A1 (en) * | 2017-03-22 | 2018-09-27 | The Board Of Trustees Of The Leland Stanford Junior University | Molecular profiling using proximity ligation-in situ hybridization |
| EP3447152A1 (en) | 2017-08-25 | 2019-02-27 | Eawag, Swiss Federal Institute of Aquatic Science and Technology | Accurate and massively parallel quantification of nucleic acid |
-
2021
- 2021-03-18 DK DK21163300.3T patent/DK4060050T3/da active
- 2021-03-18 FI FIEP21163300.3T patent/FI4060050T3/fi active
- 2021-03-18 PT PT211633003T patent/PT4060050T/pt unknown
- 2021-03-18 EP EP21163300.3A patent/EP4060050B1/en active Active
- 2021-03-18 ES ES21163300T patent/ES2942546T3/es active Active
-
2022
- 2022-01-21 EP EP22152617.1A patent/EP4060053A1/en active Pending
- 2022-02-16 CA CA3149025A patent/CA3149025A1/en active Pending
- 2022-03-03 US US17/685,964 patent/US11486003B2/en active Active
- 2022-03-10 CN CN202210231400.8A patent/CN115109842A/zh active Pending
- 2022-03-10 KR KR1020220030355A patent/KR20220130592A/ko active Pending
- 2022-03-11 JP JP2022038217A patent/JP7651497B2/ja active Active
- 2022-03-17 TW TW111109836A patent/TWI864378B/zh active
-
2024
- 2024-12-05 JP JP2024212058A patent/JP2025029149A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| TW202305143A (zh) | 2023-02-01 |
| EP4060050B1 (en) | 2023-02-15 |
| PT4060050T (pt) | 2023-05-02 |
| EP4060050A1 (en) | 2022-09-21 |
| JP2025029149A (ja) | 2025-03-05 |
| FI4060050T3 (fi) | 2023-05-04 |
| TWI864378B (zh) | 2024-12-01 |
| CN115109842A (zh) | 2022-09-27 |
| CA3149025A1 (en) | 2022-09-18 |
| DK4060050T3 (da) | 2023-03-27 |
| US20220298569A1 (en) | 2022-09-22 |
| KR20220130592A (ko) | 2022-09-27 |
| US11486003B2 (en) | 2022-11-01 |
| JP7651497B2 (ja) | 2025-03-26 |
| JP2022145606A (ja) | 2022-10-04 |
| EP4060053A1 (en) | 2022-09-21 |
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