ES2936844T3 - Sistema de electroforesis en gel para electroforesis en gel unicelular - Google Patents
Sistema de electroforesis en gel para electroforesis en gel unicelular Download PDFInfo
- Publication number
- ES2936844T3 ES2936844T3 ES16715229T ES16715229T ES2936844T3 ES 2936844 T3 ES2936844 T3 ES 2936844T3 ES 16715229 T ES16715229 T ES 16715229T ES 16715229 T ES16715229 T ES 16715229T ES 2936844 T3 ES2936844 T3 ES 2936844T3
- Authority
- ES
- Spain
- Prior art keywords
- gel electrophoresis
- support plate
- chamber
- support
- electrophoresis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001502 gel electrophoresis Methods 0.000 title claims abstract description 93
- 238000003927 comet assay Methods 0.000 title claims abstract description 27
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- 239000000463 material Substances 0.000 claims description 11
- 231100000170 comet assay Toxicity 0.000 claims description 9
- 238000001816 cooling Methods 0.000 claims description 7
- 239000003513 alkali Substances 0.000 claims description 6
- 230000005660 hydrophilic surface Effects 0.000 claims description 5
- 229920006267 polyester film Polymers 0.000 claims description 5
- 230000020169 heat generation Effects 0.000 claims description 4
- 238000012805 post-processing Methods 0.000 claims description 2
- 238000001962 electrophoresis Methods 0.000 description 83
- 239000000499 gel Substances 0.000 description 66
- 125000006850 spacer group Chemical group 0.000 description 44
- 239000002585 base Substances 0.000 description 14
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- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
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- 231100000025 genetic toxicology Toxicity 0.000 description 1
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- 231100000350 mutagenesis Toxicity 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/54—Supports specially adapted for pipettes and burettes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44708—Cooling
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/56—Means for indicating position of a recipient or sample in an array
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH3132015 | 2015-03-06 | ||
| PCT/CH2016/000043 WO2016141495A2 (de) | 2015-03-06 | 2016-03-07 | Gelelektrophorese-system für einzelzell-gelelektrophorese |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2936844T3 true ES2936844T3 (es) | 2023-03-22 |
Family
ID=55699274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES16715229T Active ES2936844T3 (es) | 2015-03-06 | 2016-03-07 | Sistema de electroforesis en gel para electroforesis en gel unicelular |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US10591439B2 (enExample) |
| EP (1) | EP3265796B1 (enExample) |
| JP (1) | JP6875052B2 (enExample) |
| CN (1) | CN107430087B (enExample) |
| ES (1) | ES2936844T3 (enExample) |
| PL (1) | PL3265796T3 (enExample) |
| WO (1) | WO2016141495A2 (enExample) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| USD840053S1 (en) * | 2017-01-25 | 2019-02-05 | Heathrow Scientific Llc | Test tube holding device |
| CH713402A1 (de) * | 2017-01-30 | 2018-07-31 | 4D Lifetec Ag | Trägerplatte für Laborgeräte. |
| EP3508841A1 (en) | 2018-01-08 | 2019-07-10 | 4D Lifetec AG | Single cell gel electrophoresis |
| CN211292712U (zh) * | 2019-12-16 | 2020-08-18 | 江苏悠然生物科技有限公司 | 电泳预制胶基片用结合结构 |
| US20230383362A1 (en) | 2020-10-22 | 2023-11-30 | 4D Lifetec Ag | Dna damage sensitivity as a new biomarker for detection of cancer and cancer susceptibility |
| TR202018906A2 (tr) * | 2020-11-24 | 2022-06-21 | Trakya Ueniversitesi Rektoerluegue | Tek hücre jel elektroforezi̇ akiş si̇tometri̇ |
| CN116136511A (zh) * | 2021-11-17 | 2023-05-19 | 中国科学院大连化学物理研究所 | 一种具有防脱落固定式彗星电泳装置及使用方法 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5165898A (en) * | 1986-08-13 | 1992-11-24 | The Board Of Trustees Of The Leland Stanford Junior University | Electrophoresis using contour-clamped electric fields |
| US4775003A (en) * | 1987-03-27 | 1988-10-04 | Koh John K | Laboratory materials and apparatus support and laminar flow thermal transfer device |
| GB9000444D0 (en) * | 1990-01-09 | 1990-03-07 | Hybaid Ltd | Analysis of biological molecule samples |
| GB9024428D0 (en) * | 1990-11-09 | 1991-01-02 | Elchrom Ltd | Apparatus and method for submerged gel electrophoresis |
| US5158661A (en) * | 1991-03-22 | 1992-10-27 | Fotodyne Incorporated | Electrophoresis temperature control apparatus |
| CN100410658C (zh) | 2005-09-12 | 2008-08-13 | 陕西师范大学 | 单细胞凝胶电泳装置 |
| ES2327587B1 (es) | 2006-08-11 | 2010-08-03 | Universidad Del Pais Vasco- Euskal Herriko Unibersitatea (80%) | Dispositivo parcialmente desechable para electroforesis. |
| DE102007046615B4 (de) | 2007-09-27 | 2013-12-24 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Gelträger zur Durchführung des COMET-Assays mit Automatisierungsmöglichkeit im Roboter-gestützten Analysebetrieb zur Realisierung eines Hochdurchsatz-Verfahrens |
| JP5135530B2 (ja) * | 2008-04-14 | 2013-02-06 | フォレクシー | 非浸漬電気泳動のための装置及び方法 |
| US20090272657A1 (en) * | 2008-05-01 | 2009-11-05 | Massachusetts Institute Of Technology | Devices and processes for analyzing nucleic acid damage and repair using electrophoresis |
| EP2484749A1 (en) | 2011-02-07 | 2012-08-08 | Aarhus Universitet | Gel electrophoresis assay system |
| US9128046B2 (en) * | 2011-10-31 | 2015-09-08 | Cellomics, Inc. | Slide holder assembly for comet assay |
| CN202720211U (zh) | 2012-07-27 | 2013-02-06 | 宁波出入境检验检疫局检验检疫技术中心 | 彗星试验装置 |
| KR20140123243A (ko) * | 2013-04-12 | 2014-10-22 | 주식회사 나노엔텍 | 단일세포 전기영동측정칩, 칩 홀딩유닛 및 단일세포 전기영동처리장치 |
| WO2015079048A1 (en) | 2013-11-29 | 2015-06-04 | Ge Healthcare Bio-Sciences Ab | Electrophoresis system |
-
2016
- 2016-03-07 PL PL16715229.7T patent/PL3265796T3/pl unknown
- 2016-03-07 ES ES16715229T patent/ES2936844T3/es active Active
- 2016-03-07 JP JP2017543818A patent/JP6875052B2/ja active Active
- 2016-03-07 CN CN201680013900.7A patent/CN107430087B/zh active Active
- 2016-03-07 WO PCT/CH2016/000043 patent/WO2016141495A2/de not_active Ceased
- 2016-03-07 EP EP16715229.7A patent/EP3265796B1/de active Active
- 2016-03-07 US US15/554,582 patent/US10591439B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20180095055A1 (en) | 2018-04-05 |
| EP3265796A2 (de) | 2018-01-10 |
| JP6875052B2 (ja) | 2021-05-19 |
| CN107430087B (zh) | 2020-11-10 |
| CN107430087A (zh) | 2017-12-01 |
| PL3265796T3 (pl) | 2023-03-20 |
| US10591439B2 (en) | 2020-03-17 |
| WO2016141495A2 (de) | 2016-09-15 |
| EP3265796B1 (de) | 2022-11-09 |
| JP2018508779A (ja) | 2018-03-29 |
| WO2016141495A3 (de) | 2016-11-03 |
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