ES2721535T3 - Procedimiento de purificación de virus a gran escala - Google Patents
Procedimiento de purificación de virus a gran escala Download PDFInfo
- Publication number
- ES2721535T3 ES2721535T3 ES15816413T ES15816413T ES2721535T3 ES 2721535 T3 ES2721535 T3 ES 2721535T3 ES 15816413 T ES15816413 T ES 15816413T ES 15816413 T ES15816413 T ES 15816413T ES 2721535 T3 ES2721535 T3 ES 2721535T3
- Authority
- ES
- Spain
- Prior art keywords
- virus
- gradient
- purification procedure
- virus purification
- percentage
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
- C12N7/02—Recovery or purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16051—Methods of production or purification of viral material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Un procedimiento de purificación de un virus que comprende al menos las siguientes etapas: a) obtener un fluido que contiene virus, b) proporcionar un gradiente de densidad lineal preformado de un intervalo dado X% -Y% (v/p) (siendo X el límite inferior y Y el límite superior) en un rotor de centrífuga con una capacidad de al menos 8 L, c) agregar el fluido al gradiente de densidad lineal preformado, d) centrifugar para separar el virus de las impurezas contaminantes, y e) recolectar las fracciones que comprenden el virus purificado, en el que la parte lineal del gradiente del intervalo dado X%-Y% abarca el porcentaje de densidad correspondiente al porcentaje, en el gradiente, en el que el virus que se purificará migrará.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462092413P | 2014-12-16 | 2014-12-16 | |
| PCT/EP2015/079534 WO2016096688A1 (en) | 2014-12-16 | 2015-12-14 | A method for a large scale virus purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2721535T3 true ES2721535T3 (es) | 2019-08-01 |
Family
ID=55024091
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES15816413T Active ES2721535T3 (es) | 2014-12-16 | 2015-12-14 | Procedimiento de purificación de virus a gran escala |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20170369854A1 (es) |
| EP (1) | EP3234113B1 (es) |
| JP (1) | JP6719468B2 (es) |
| BE (1) | BE1023903B1 (es) |
| ES (1) | ES2721535T3 (es) |
| WO (1) | WO2016096688A1 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949671A (zh) * | 2018-08-02 | 2018-12-07 | 重庆市畜牧科学院 | 鹌鹑成纤维细胞培养基及其制备方法 |
| CN113025584A (zh) * | 2019-12-09 | 2021-06-25 | 上海青赛生物科技有限公司 | 狂犬病毒纯化方法与应用 |
| CN112704733A (zh) * | 2020-12-29 | 2021-04-27 | 深圳康泰生物制品股份有限公司 | 四价流感病毒鸡胚尿囊液纯化工艺、四价流感病毒裂解疫苗及其制备方法 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5310356Y2 (es) * | 1973-08-03 | 1978-03-18 | ||
| US4118477A (en) * | 1977-02-14 | 1978-10-03 | Merck & Co., Inc. | Hepatitis B antigen |
| DK0382271T3 (da) | 1989-02-04 | 1995-05-01 | Akzo Nobel Nv | Tocoler som adjuvanser i vacciner |
| FR2808803B1 (fr) | 2000-05-11 | 2004-12-10 | Agronomique Inst Nat Rech | Cellules es modifiees et gene specifique de cellules es |
| GB0024089D0 (en) | 2000-10-02 | 2000-11-15 | Smithkline Beecham Biolog | Novel compounds |
| EP1528101A1 (en) | 2003-11-03 | 2005-05-04 | ProBioGen AG | Immortalized avian cell lines for virus production |
| FR2884255B1 (fr) | 2005-04-11 | 2010-11-05 | Vivalis | Utilisation de lignees de cellules souches aviaires ebx pour la production de vaccin contre la grippe |
| CN104694489A (zh) | 2007-05-04 | 2015-06-10 | 巴克斯特国际公司 | 用于病毒纯化用连续超速离心的糖溶液配方 |
| FR2918074B1 (fr) | 2007-06-27 | 2009-10-09 | Sanofi Pasteur | Procede de purification d'une suspension virale. |
| EP2393922A1 (en) * | 2009-02-06 | 2011-12-14 | GlaxoSmithKline Biologicals S.A. | Purification of virus or viral antigens by density gradient ultracentrifugation |
-
2015
- 2015-12-14 US US15/534,903 patent/US20170369854A1/en not_active Abandoned
- 2015-12-14 JP JP2017531819A patent/JP6719468B2/ja active Active
- 2015-12-14 BE BE2015/5818A patent/BE1023903B1/fr not_active IP Right Cessation
- 2015-12-14 ES ES15816413T patent/ES2721535T3/es active Active
- 2015-12-14 WO PCT/EP2015/079534 patent/WO2016096688A1/en active Application Filing
- 2015-12-14 EP EP15816413.7A patent/EP3234113B1/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016096688A1 (en) | 2016-06-23 |
| US20170369854A1 (en) | 2017-12-28 |
| JP6719468B2 (ja) | 2020-07-08 |
| BE1023903A1 (fr) | 2017-09-07 |
| EP3234113B1 (en) | 2019-01-23 |
| JP2018500023A (ja) | 2018-01-11 |
| BE1023903B1 (fr) | 2017-09-08 |
| EP3234113A1 (en) | 2017-10-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK3394393T3 (da) | Olie-, vand-, gas- og faststofpartikelseparering inden for olie- og/eller gasproduktion | |
| BR112015016136A2 (pt) | métodos para isolar microvesículas | |
| CR20160078A (es) | Asiento para bicicleta | |
| BR112014032177A2 (pt) | separador | |
| CL2017000404A1 (es) | Rueda pelton | |
| AR091538A1 (es) | Purificacion de particulas tipo virus | |
| JP2016182547A5 (es) | ||
| UA115769C2 (uk) | Пристрій для відведення текучого середовища пристроєм центрифугування | |
| ES2721535T3 (es) | Procedimiento de purificación de virus a gran escala | |
| AR089463A1 (es) | Metodo y aparato para retener una brida en un medidor de flujo | |
| AR097627A1 (es) | Métodos y dispositivos para la codificación conjunta de canales múltiples | |
| DK3440310T3 (da) | Faststofpartikelseparering inden for olie- og/eller gasproduktion | |
| TW201614209A (en) | Collection component and sample processing kit having the same | |
| DK3242933T3 (da) | Genetisk modificeret mesenkymale stamceller udtrykkende alfa-1 antitrypsin (AAT) | |
| AR104737A1 (es) | Reconfiguración en una red de malla inalámbrica | |
| DK3548099T3 (da) | Ovn til kontinuerlig fjernelse af plantesundhedsmæssige skadevoldere, der forekommer i organiske partikler af vegetabilsk oprindelse | |
| FR3007038B1 (fr) | Polypeptide ayant la capacite de former des branchements d'unites glucosyle en alpha-1,3 sur un accepteur | |
| BR112015020041A2 (pt) | coletor de pó, e, método de remoção de partículas do ar sujo | |
| ES2466571A8 (es) | Membrana cerámica de filtración | |
| AR102853A1 (es) | Purificación de gelatina | |
| CL2018000076A1 (es) | Mejora del metabolismo de las microalgas de xilosa. | |
| EA201991117A1 (ru) | Системы и способы улучшенного поддержания высокоэффективной конфигурации с обращенным полем с вакуумированием с захватом многомасштабного типа | |
| EA201692555A1 (ru) | Применение декстрансульфата, имеющего среднюю молекулярную массу менее 10000 да, для индуцирования ангиогенеза у субъекта | |
| MY179359A (en) | Methods for purification of a virus produced in vitro and clearance assay for the virus | |
| JP2017535495A5 (es) |