ES2721155T3 - Copolímeros para precipitación de proteínas - Google Patents

Copolímeros para precipitación de proteínas Download PDF

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Publication number
ES2721155T3
ES2721155T3 ES13795434T ES13795434T ES2721155T3 ES 2721155 T3 ES2721155 T3 ES 2721155T3 ES 13795434 T ES13795434 T ES 13795434T ES 13795434 T ES13795434 T ES 13795434T ES 2721155 T3 ES2721155 T3 ES 2721155T3
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ES
Spain
Prior art keywords
sample
acid
target molecule
group
precipitate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES13795434T
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English (en)
Inventor
Johann Bauer
Almut Rapp
Bernd Stanislawski
Florian Capito
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Merck Patent GmbH
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Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Publication of ES2721155T3 publication Critical patent/ES2721155T3/es
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)

Abstract

Método de aislamiento de una molécula diana a partir de una muestra que comprende: a) proporcionar la muestra b) mezclar uno o más copolímeros sintetizados a partir de unidades monoméricas de manera que cada unidad monomérica que se usa para sintetizar el copolímero tenga al menos un grupo aniónico o hidrófobo y la unidad monomérica que tiene al menos un grupo esté seleccionada entre bencilacrilamida (BzAAm), metacrilato de bencilo (BzMA), N-isopropilacrilamida (NIPAM), metacrilato de metilo (MMA), acrilato de butilo, acrilato de tercbutilo o ácido 4-(acriloilamido)benzoico (4-ABZ) y la unidad monomérica que tiene al menos un grupo aniónico está seleccionada entre ácido 2-acrilamido-2-metilpropano (AMPS), ácido vinilsulfónico (VS), ácido estiren sulfónico o ácido (met)acrílico, con la muestra de manera que se forma un precipitado de copolímero-molécula diana c) separar el precipitado de la mezcla de la etapa b) de modo que en la etapa a) el pH de la muestra se ajusta a un pH por debajo del punto isoeléctrico de la molécula diana.
ES13795434T 2012-12-20 2013-11-25 Copolímeros para precipitación de proteínas Active ES2721155T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12008475 2012-12-20
PCT/EP2013/003552 WO2014094957A1 (en) 2012-12-20 2013-11-25 Copolymers for protein precipitation

Publications (1)

Publication Number Publication Date
ES2721155T3 true ES2721155T3 (es) 2019-07-29

Family

ID=47519791

Family Applications (1)

Application Number Title Priority Date Filing Date
ES13795434T Active ES2721155T3 (es) 2012-12-20 2013-11-25 Copolímeros para precipitación de proteínas

Country Status (9)

Country Link
US (1) US9926341B2 (es)
EP (1) EP2935309B1 (es)
JP (1) JP6336473B2 (es)
KR (1) KR102097954B1 (es)
CN (1) CN104854123B (es)
CA (1) CA2895584A1 (es)
ES (1) ES2721155T3 (es)
SG (1) SG11201504649RA (es)
WO (1) WO2014094957A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018517559A (ja) 2015-06-05 2018-07-05 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 吸着性バイオプロセス清澄化剤並びにその製造及び使用方法
JP7006318B2 (ja) * 2018-01-31 2022-01-24 株式会社リコー 活性エネルギー線硬化型インク、活性エネルギー線硬化型インクジェットインク、組成物収容容器、２次元または３次元の像形成装置及び像形成方法、硬化物、硬化物の製造方法及び加飾体の製造方法
CN108395567B (zh) * 2018-04-17 2020-07-14 兰州大学 整体凝胶复合材料制备及用于高效分离人血清中蛋白质和小分子物质
EP3816177A4 (en) * 2018-06-29 2021-12-01 Mitsubishi Chemical Corporation POLYPEPTIDE ISOLATION PROCESS, POLYPEPTIDE PRODUCTION PROCESS AND POLYPEPTIDE PURIFICATION APPARATUS
JP6564539B1 (ja) * 2018-09-14 2019-08-21 長瀬産業株式会社 スルホン酸化合物によるペプチド精製方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5360710A (en) * 1992-05-06 1994-11-01 Eastman Kodak Company Color photographic materials containing polymeric couplers
US5922531A (en) * 1994-06-21 1999-07-13 Advanced Research And Technology Polyelectrolyte treated glass for enzyme immobilization and protein purification
US6307013B1 (en) 1998-07-21 2001-10-23 Monsanto Technology Llc Clarification of protein precipitate suspensions using anionic polymeric flocculants
JP2007528784A (ja) * 2004-02-11 2007-10-18 マサチューセッツ・インスティチュート・オブ・テクノロジー 多層ポリマー被覆磁性ナノクラスタ
US8163886B2 (en) 2006-12-21 2012-04-24 Emd Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
EP2120915B1 (en) 2007-01-22 2011-09-28 Genentech, Inc. Polyelectrolyte precipitation and purification of antibodies
EP3597671B1 (en) 2010-05-17 2022-09-21 EMD Millipore Corporation Stimulus responsive polymers for the purification of biomolecules

Also Published As

Publication number Publication date
CN104854123A (zh) 2015-08-19
JP6336473B2 (ja) 2018-06-06
CN104854123B (zh) 2020-04-21
CA2895584A1 (en) 2014-06-26
SG11201504649RA (en) 2015-07-30
US9926341B2 (en) 2018-03-27
WO2014094957A1 (en) 2014-06-26
JP2016501275A (ja) 2016-01-18
EP2935309A1 (en) 2015-10-28
KR102097954B1 (ko) 2020-04-07
US20150329589A1 (en) 2015-11-19
EP2935309B1 (en) 2019-01-23
KR20150096772A (ko) 2015-08-25

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