ES2713153T3 - Métodos y composiciones que utilizan la transposición unilateral - Google Patents
Métodos y composiciones que utilizan la transposición unilateral Download PDFInfo
- Publication number
- ES2713153T3 ES2713153T3 ES15734543T ES15734543T ES2713153T3 ES 2713153 T3 ES2713153 T3 ES 2713153T3 ES 15734543 T ES15734543 T ES 15734543T ES 15734543 T ES15734543 T ES 15734543T ES 2713153 T3 ES2713153 T3 ES 2713153T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- nucleic acids
- transposon
- target nucleic
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462019209P | 2014-06-30 | 2014-06-30 | |
| PCT/US2015/038050 WO2016003814A1 (en) | 2014-06-30 | 2015-06-26 | Methods and compositions using one-sided transposition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2713153T3 true ES2713153T3 (es) | 2019-05-20 |
Family
ID=53514440
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES15734543T Active ES2713153T3 (es) | 2014-06-30 | 2015-06-26 | Métodos y composiciones que utilizan la transposición unilateral |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US10577603B2 (https=) |
| EP (1) | EP3161152B1 (https=) |
| JP (1) | JP6652512B2 (https=) |
| CN (2) | CN106661561B (https=) |
| AU (1) | AU2015284464B2 (https=) |
| CA (1) | CA2953791A1 (https=) |
| DK (1) | DK3161152T3 (https=) |
| ES (1) | ES2713153T3 (https=) |
| SG (1) | SG11201610910QA (https=) |
| WO (1) | WO2016003814A1 (https=) |
Families Citing this family (81)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5843614B2 (ja) | 2009-01-30 | 2016-01-13 | オックスフォード ナノポア テクノロジーズ リミテッド | 膜貫通配列決定における核酸構築物のためのアダプター |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| HRP20151294T1 (hr) | 2010-04-05 | 2016-03-11 | Prognosys Biosciences, Inc. | Biološka testiranja sa prostornim kodiranjem |
| AU2012288629B2 (en) | 2011-07-25 | 2017-02-02 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
| US11155860B2 (en) | 2012-07-19 | 2021-10-26 | Oxford Nanopore Technologies Ltd. | SSB method |
| WO2014060483A1 (en) | 2012-10-17 | 2014-04-24 | Spatial Transcriptomics Ab | Methods and product for optimising localised or spatial detection of gene expression in a tissue sample |
| KR102168813B1 (ko) | 2013-03-08 | 2020-10-22 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 효소 정지 방법 |
| GB201314695D0 (en) | 2013-08-16 | 2013-10-02 | Oxford Nanopore Tech Ltd | Method |
| EP3013983B1 (en) | 2013-06-25 | 2023-02-15 | Prognosys Biosciences, Inc. | Spatially encoded biological assays using a microfluidic device |
| GB201403096D0 (en) | 2014-02-21 | 2014-04-09 | Oxford Nanopore Tech Ltd | Sample preparation method |
| GB201418159D0 (en) | 2014-10-14 | 2014-11-26 | Oxford Nanopore Tech Ltd | Method |
| US20180010120A1 (en) | 2015-01-16 | 2018-01-11 | Seqwell, Inc. | Normalized iterative barcoding and sequencing of dna collections |
| US10774374B2 (en) | 2015-04-10 | 2020-09-15 | Spatial Transcriptomics AB and Illumina, Inc. | Spatially distinguished, multiplex nucleic acid analysis of biological specimens |
| US20170283864A1 (en) * | 2016-03-31 | 2017-10-05 | Agilent Technologies, Inc. | Use of transposase and y adapters to fragment and tag dna |
| US11932847B2 (en) * | 2016-04-19 | 2024-03-19 | Kapa Biosystems, Inc. | Transposase competitor control system |
| GB201609220D0 (en) | 2016-05-25 | 2016-07-06 | Oxford Nanopore Tech Ltd | Method |
| US10240196B2 (en) | 2016-05-27 | 2019-03-26 | Agilent Technologies, Inc. | Transposase-random priming DNA sample preparation |
| CN117822128A (zh) | 2016-07-22 | 2024-04-05 | 俄勒冈健康与科学大学 | 单细胞全基因组文库及制备其的组合索引方法 |
| KR20250143875A (ko) | 2017-02-21 | 2025-10-02 | 일루미나, 인코포레이티드 | 링커를 갖는 고정된 트랜스포좀을 사용한 태그먼트화 |
| KR20200004335A (ko) | 2017-05-05 | 2020-01-13 | 스키피오 바이오사이언스 | 하이드로겔 내의 별개의 생물학적 단위를 트래핑하고 바코딩하는 방법 |
| WO2018217625A1 (en) * | 2017-05-23 | 2018-11-29 | Bio-Rad Laboratories, Inc. | Molecular barcoding |
| GB201807793D0 (en) | 2018-05-14 | 2018-06-27 | Oxford Nanopore Tech Ltd | Method |
| IL271454B2 (en) | 2018-05-17 | 2025-04-01 | Illumina Inc | Rapid single-cell genetic sequencing with low Aggregation bias |
| US11519033B2 (en) | 2018-08-28 | 2022-12-06 | 10X Genomics, Inc. | Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample |
| CN112739829A (zh) * | 2018-09-27 | 2021-04-30 | 深圳华大生命科学研究院 | 测序文库的构建方法和得到的测序文库及测序方法 |
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| US12529094B2 (en) | 2018-12-10 | 2026-01-20 | 10X Genomics, Inc. | Imaging system hardware |
| JP7571012B2 (ja) | 2018-12-17 | 2024-10-22 | イルミナ インコーポレイテッド | 配列決定用ライブラリーを調製するための方法および手段 |
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| US11926867B2 (en) | 2019-01-06 | 2024-03-12 | 10X Genomics, Inc. | Generating capture probes for spatial analysis |
| SG11202102801TA (en) | 2019-04-29 | 2021-04-29 | Illumina Inc | Identification and analysis of microbial samples by rapid incubation and nucleic acid enrichment |
| EP3976820A1 (en) | 2019-05-30 | 2022-04-06 | 10X Genomics, Inc. | Methods of detecting spatial heterogeneity of a biological sample |
| MX2021007774A (es) | 2019-07-12 | 2021-08-05 | Illumina Cambridge Ltd | Preparacion de genoteca de acido nucleico usando electroforesis. |
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| WO2021092433A2 (en) | 2019-11-08 | 2021-05-14 | 10X Genomics, Inc. | Enhancing specificity of analyte binding |
| EP4081656A1 (en) | 2019-12-23 | 2022-11-02 | 10X Genomics, Inc. | Compositions and methods for using fixed biological samples in partition-based assays |
| CN115135984B (zh) | 2019-12-23 | 2025-12-23 | 10X基因组学有限公司 | 可逆固定试剂及其使用方法 |
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| US12365942B2 (en) | 2020-01-13 | 2025-07-22 | 10X Genomics, Inc. | Methods of decreasing background on a spatial array |
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| US11732299B2 (en) | 2020-01-21 | 2023-08-22 | 10X Genomics, Inc. | Spatial assays with perturbed cells |
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