ES2692726T3 - Formulaciones para la estabilización de ácidos nucleicos en sustratos sólidos - Google Patents
Formulaciones para la estabilización de ácidos nucleicos en sustratos sólidos Download PDFInfo
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- ES2692726T3 ES2692726T3 ES13865596.4T ES13865596T ES2692726T3 ES 2692726 T3 ES2692726 T3 ES 2692726T3 ES 13865596 T ES13865596 T ES 13865596T ES 2692726 T3 ES2692726 T3 ES 2692726T3
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- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 125000001183 hydrocarbyl group Chemical group 0.000 description 1
- 239000012948 isocyanate Substances 0.000 description 1
- 150000002513 isocyanates Chemical class 0.000 description 1
- 150000002540 isothiocyanates Chemical class 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 150000002596 lactones Chemical class 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- SXTGAOTXVOMSFW-UHFFFAOYSA-L magnesium;dithiocyanate Chemical compound [Mg+2].[S-]C#N.[S-]C#N SXTGAOTXVOMSFW-UHFFFAOYSA-L 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 108091070501 miRNA Proteins 0.000 description 1
- 239000002679 microRNA Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 150000002923 oximes Chemical class 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical class OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229940116357 potassium thiocyanate Drugs 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- KCXFHTAICRTXLI-UHFFFAOYSA-N propane-1-sulfonic acid Chemical compound CCCS(O)(=O)=O KCXFHTAICRTXLI-UHFFFAOYSA-N 0.000 description 1
- 230000001012 protector Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 206010040882 skin lesion Diseases 0.000 description 1
- 231100000444 skin lesion Toxicity 0.000 description 1
- 239000004055 small Interfering RNA Substances 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 125000005504 styryl group Chemical group 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 150000008053 sultones Chemical class 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- DHCDFWKWKRSZHF-UHFFFAOYSA-L thiosulfate(2-) Chemical compound [O-]S([S-])(=O)=O DHCDFWKWKRSZHF-UHFFFAOYSA-L 0.000 description 1
- 238000005809 transesterification reaction Methods 0.000 description 1
- 229910052723 transition metal Inorganic materials 0.000 description 1
- 150000003624 transition metals Chemical class 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
- MLVWCBYTEFCFSG-UHFFFAOYSA-L zinc;dithiocyanate Chemical compound [Zn+2].[S-]C#N.[S-]C#N MLVWCBYTEFCFSG-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/721,948 US9040675B2 (en) | 2012-04-30 | 2012-12-20 | Formulations for nucleic acid stabilization on solid substrates |
| US201213721948 | 2012-12-20 | ||
| PCT/US2013/065821 WO2014099121A1 (en) | 2012-12-20 | 2013-10-21 | Formulations for nucleic acid stabilization on solid substrates |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2692726T3 true ES2692726T3 (es) | 2018-12-04 |
Family
ID=50978990
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES13865596.4T Active ES2692726T3 (es) | 2012-12-20 | 2013-10-21 | Formulaciones para la estabilización de ácidos nucleicos en sustratos sólidos |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP2935583B1 (enExample) |
| JP (1) | JP6544860B2 (enExample) |
| ES (1) | ES2692726T3 (enExample) |
| WO (1) | WO2014099121A1 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201515355D0 (en) * | 2015-08-28 | 2015-10-14 | Ge Healthcare Ltd | A method and kit for analyte detection |
| ES2912965T3 (es) | 2015-09-09 | 2022-05-30 | Drawbridge Health Inc | Dispositivos para la recopilación, estabilización y conservación de muestras |
| EP3528950A4 (en) * | 2016-10-24 | 2020-05-27 | Biomatrica, INC. | STABILIZATION OF NUCLEIC ACIDS ON PAPER |
| CN210383905U (zh) | 2017-01-10 | 2020-04-24 | 集联健康有限公司 | 一种用于从受试者收集流体样品的装置以及运输套筒 |
| CN112662663B (zh) * | 2021-01-27 | 2022-11-08 | 苏州赛普生物科技有限公司 | 一种适于常温运输的rna提取试剂盒及提取方法 |
| CN115074356A (zh) * | 2022-08-18 | 2022-09-20 | 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） | 一种新冠病毒核酸提取促进剂及其应用 |
| EP4495250A1 (en) * | 2023-07-18 | 2025-01-22 | Centogene GmbH | Method for preparing an rna preparation and use thereof |
| SE2351278A1 (en) * | 2023-11-08 | 2025-05-09 | Sequrna Ab | Dry storage |
| CN120741115B (zh) * | 2025-09-04 | 2025-11-18 | 宁波天润生物药业有限公司 | 一种血清预埋方法及其应用 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5496562A (en) * | 1988-10-05 | 1996-03-05 | Flinders Technologies Pty Ltd | Solid medium and method for DNA storage |
| DE96915910T1 (de) * | 1995-06-07 | 2004-04-22 | Whatman Plc, Maidstone | Trockenes festes medium zur lagerung und analyse von genetischem material |
| US5939259A (en) * | 1997-04-09 | 1999-08-17 | Schleicher & Schuell, Inc. | Methods and devices for collecting and storing clinical samples for genetic analysis |
| AU2002365229A1 (en) * | 2001-11-15 | 2003-09-02 | Whatman, Inc. | Methods and materials for detecting genetic material |
| US20040126783A1 (en) * | 2002-07-10 | 2004-07-01 | Massachusetts Institute Of Technology | Apparatus and method for isolating a nucleic acid from a sample |
| EP1590362B8 (en) * | 2003-01-29 | 2015-06-03 | Epigenomics AG | Improved method for bisulfite treatment |
| JP3714940B2 (ja) * | 2003-11-07 | 2005-11-09 | 株式会社日立ハイテクノロジーズ | Ｒｎａ抽出方法、および生体材料の分析方法 |
| EP1584923A3 (en) * | 2004-04-07 | 2006-01-04 | Roche Diagnostics GmbH | Stabilization of biomolecules in samples |
| GB0505909D0 (en) * | 2005-03-23 | 2005-04-27 | Univ Leeds | Formulations |
| CA2756820A1 (en) * | 2009-04-17 | 2010-10-21 | Colby Pharmaceutical Company | Pharmaceutically active compositions comprising oxidative stress modulators (osm), new chemical entities, compositions and uses |
| US8222397B2 (en) * | 2009-08-28 | 2012-07-17 | Promega Corporation | Methods of optimal purification of nucleic acids and kit for use in performing such methods |
| EP2388312A1 (en) * | 2010-05-17 | 2011-11-23 | Curetis AG | Universally applicable lysis buffer and processing methods for the lysis of bodily samples |
| WO2012018638A2 (en) * | 2010-07-26 | 2012-02-09 | Biomatrica, Inc. | Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures |
-
2013
- 2013-10-21 EP EP13865596.4A patent/EP2935583B1/en active Active
- 2013-10-21 ES ES13865596.4T patent/ES2692726T3/es active Active
- 2013-10-21 WO PCT/US2013/065821 patent/WO2014099121A1/en not_active Ceased
- 2013-10-21 JP JP2015549377A patent/JP6544860B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2935583A1 (en) | 2015-10-28 |
| JP2016505262A (ja) | 2016-02-25 |
| WO2014099121A1 (en) | 2014-06-26 |
| EP2935583B1 (en) | 2018-09-12 |
| JP6544860B2 (ja) | 2019-07-17 |
| EP2935583A4 (en) | 2016-07-27 |
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