ES2669021T3 - Procedimiento para la generación de muestras de elevado título del virus de la hepatitis E y ensayo de titulación para el virus de la hepatitis E - Google Patents

Procedimiento para la generación de muestras de elevado título del virus de la hepatitis E y ensayo de titulación para el virus de la hepatitis E Download PDF

Info

Publication number
ES2669021T3
ES2669021T3 ES16178844.3T ES16178844T ES2669021T3 ES 2669021 T3 ES2669021 T3 ES 2669021T3 ES 16178844 T ES16178844 T ES 16178844T ES 2669021 T3 ES2669021 T3 ES 2669021T3
Authority
ES
Spain
Prior art keywords
hev
approximately
vhe
reaction mixture
copies
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES16178844.3T
Other languages
English (en)
Spanish (es)
Inventor
Brett Buno
Terri Journigan
Joann Hotta
Michael Burdick
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Grifols SA
Original Assignee
Grifols SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Grifols SA filed Critical Grifols SA
Application granted granted Critical
Publication of ES2669021T3 publication Critical patent/ES2669021T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/05—Adjuvants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/28011—Hepeviridae
    • C12N2770/28111—Hepevirus, e.g. hepatitis E virus
    • C12N2770/28121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/28011—Hepeviridae
    • C12N2770/28111—Hepevirus, e.g. hepatitis E virus
    • C12N2770/28151—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Communicable Diseases (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Oncology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
ES16178844.3T 2015-07-23 2016-07-11 Procedimiento para la generación de muestras de elevado título del virus de la hepatitis E y ensayo de titulación para el virus de la hepatitis E Active ES2669021T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562195936P 2015-07-23 2015-07-23
US201562195936P 2015-07-23

Publications (1)

Publication Number Publication Date
ES2669021T3 true ES2669021T3 (es) 2018-05-23

Family

ID=56409494

Family Applications (1)

Application Number Title Priority Date Filing Date
ES16178844.3T Active ES2669021T3 (es) 2015-07-23 2016-07-11 Procedimiento para la generación de muestras de elevado título del virus de la hepatitis E y ensayo de titulación para el virus de la hepatitis E

Country Status (22)

Country Link
US (1) US10208291B2 (cg-RX-API-DMAC7.html)
EP (1) EP3121267B1 (cg-RX-API-DMAC7.html)
JP (1) JP6588397B2 (cg-RX-API-DMAC7.html)
KR (1) KR102154380B1 (cg-RX-API-DMAC7.html)
CN (1) CN106367397B (cg-RX-API-DMAC7.html)
AR (1) AR106015A1 (cg-RX-API-DMAC7.html)
AU (1) AU2016204734B2 (cg-RX-API-DMAC7.html)
BR (1) BR102016016677A2 (cg-RX-API-DMAC7.html)
CA (1) CA2937088C (cg-RX-API-DMAC7.html)
CL (1) CL2016001768A1 (cg-RX-API-DMAC7.html)
ES (1) ES2669021T3 (cg-RX-API-DMAC7.html)
HU (1) HUE036699T2 (cg-RX-API-DMAC7.html)
IL (1) IL246634B (cg-RX-API-DMAC7.html)
MX (1) MX375106B (cg-RX-API-DMAC7.html)
MY (1) MY176204A (cg-RX-API-DMAC7.html)
NZ (1) NZ722213A (cg-RX-API-DMAC7.html)
PL (1) PL3121267T3 (cg-RX-API-DMAC7.html)
PT (1) PT3121267T (cg-RX-API-DMAC7.html)
RU (1) RU2691123C1 (cg-RX-API-DMAC7.html)
SG (1) SG10201605867UA (cg-RX-API-DMAC7.html)
TW (1) TWI666322B (cg-RX-API-DMAC7.html)
UY (1) UY36778A (cg-RX-API-DMAC7.html)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102158641B1 (ko) * 2019-03-06 2020-09-22 대한민국 야누스 인산화효소 저해제 및 헥사디메트린 브로마이드를 포함하는 바이러스 배양용 조성물 및 이를 이용한 바이러스 배양 방법

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6506604B2 (en) * 1993-06-11 2003-01-14 Cell Genesys, Inc. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
US6217858B1 (en) * 1997-02-11 2001-04-17 Hadasit & Medical Research Services & Development Company, Ltd. Pharmaceutical composition for treating hepatitis B virus (HBV) infection
WO2003001198A1 (en) * 2001-06-21 2003-01-03 Regents Of The University Of California Immortalization of human cells by the ectopic expression of human telomerase reverse transcriptase
WO2007046962A2 (en) * 2005-09-02 2007-04-26 The University Of Maryland, Baltimore Cell lines harboring hepatitis virus and methods for using thereof
WO2010040001A1 (en) * 2008-10-03 2010-04-08 The Rockefeller University New hcv entry factor, occludin
EP2663328B1 (en) 2011-01-10 2018-09-19 The Government of The United States of America, as represented by The Secretary, Department of Health and Human Services Infectious hepatitis e virus genotype 3 or 1 recombinants
DK2717922T3 (en) * 2011-06-10 2019-01-07 Bluebird Bio Inc GENERATIVE VECTORS FOR ADDRENOLEUCODYSTROPHY AND ADDRENOMYELONE EUROPE

Also Published As

Publication number Publication date
TW201704468A (zh) 2017-02-01
IL246634A0 (en) 2016-12-29
AU2016204734A1 (en) 2017-02-09
CA2937088C (en) 2022-06-21
CN106367397B (zh) 2021-07-20
JP2017023143A (ja) 2017-02-02
RU2691123C1 (ru) 2019-06-11
AR106015A1 (es) 2017-12-06
CL2016001768A1 (es) 2017-05-12
NZ722213A (en) 2019-04-26
HUE036699T2 (hu) 2018-07-30
BR102016016677A2 (pt) 2019-02-12
IL246634B (en) 2020-08-31
KR20170012140A (ko) 2017-02-02
PT3121267T (pt) 2018-04-26
MX375106B (es) 2025-03-06
UY36778A (es) 2016-09-30
MY176204A (en) 2020-07-24
SG10201605867UA (en) 2017-02-27
MX2016009048A (es) 2017-01-23
JP6588397B2 (ja) 2019-10-09
EP3121267A1 (en) 2017-01-25
US10208291B2 (en) 2019-02-19
KR102154380B1 (ko) 2020-09-10
AU2016204734B2 (en) 2019-11-21
EP3121267B1 (en) 2018-03-28
TWI666322B (zh) 2019-07-21
PL3121267T3 (pl) 2018-07-31
CN106367397A (zh) 2017-02-01
US20170022481A1 (en) 2017-01-26
CA2937088A1 (en) 2017-01-23

Similar Documents

Publication Publication Date Title
Zhou et al. Antiviral activities of ISG20 in positive-strand RNA virus infections
Yoo et al. Transfection of a differentiated human hepatoma cell line (Huh7) with in vitro-transcribed hepatitis C virus (HCV) RNA and establishment of a long-term culture persistently infected with HCV
Sanada et al. Transmission of HBV DNA mediated by ceramide-triggered extracellular vesicles
Tomaselli et al. ADARs and the balance game between virus infection and innate immune cell response
Kenney et al. Replacement of the hepatitis E virus ORF3 protein PxxP motif with heterologous late domain motifs affects virus release via interaction with TSG101
Yen et al. Cellular proteins specifically bind to the 5′-noncoding region of hepatitis C virus RNA
Kato et al. Systems to culture hepatitis C virus
JP7369343B2 (ja) 豚感染ウイルスの製造方法
CA2937088C (en) Method for generating high-titer hepatitis e virus stocks and titration assay for hepatitis e virus
US9284555B2 (en) Aptazyme capable of selective silencing of target miRNA by releasing an antisense sequence in hepatitis C virus-infected cells and use thereof
Otsuki et al. Elucidation of the full genetic information of Japanese rubella vaccines and the genetic changes associated with in vitro and in vivo vaccine virus phenotypes
HK1230654B (en) Method for generating high-titer hepatitis e virus stocks and titration assay for hepatitis e virus
Jin et al. Replication kinetics of coxsackievirus A16 in human rhabdomyosarcoma cells
CN108055827A (zh) 高成长肠病毒71型病毒株及其疫苗
HK1230654A1 (en) Method for generating high-titer hepatitis e virus stocks and titration assay for hepatitis e virus
Si et al. Construction of an infectious cDNA clone of a swine genotype 3 HEV strain isolated in Shanghai, China
Carreño et al. Hepatitis delta virus infection: molecular biology and treatment
Silberstein et al. Persistent growth of a human plasma-derived hepatitis C virus genotype 1b isolate in cell culture
US20130101986A1 (en) In Vitro Method for obtaining Intrahepatic Fibroblasts Infected with Hepatitis C Virus
KR101122244B1 (ko) 효율적으로 복제할 수 있는 변이 c형 간염 바이러스, 리포터 유전자를 포함하는 변이 c형 간염 바이러스, 상기 변이 c형 간염 바이러스를 이용한 c형 간염 바이러스 백신 제조방법 및 상기 변이 c형 간염 바이러스를 이용한 항c형 간염 바이러스 조성물의 스크리닝 방법
JP2003516142A (ja) ウイルスによる真核細胞のinvitro感染
Harms The role of host cellular and viral evolutionary factors influencing the replication mechanisms of chronic hepatitis E virus infection in vivo and in vitro
Ahmad et al. Diagnostic Utility of Genes Associated with SARS-CoV-2: A Review
Kachko et al. Inhibition of West Nile virus replication by short interfering RNAs.
Lister Corona Virus 229E, NL63 And OC43 Infection Of Human Monocyte-Derived Dendritic Cells: Modulation of Immune Effector Function