ES2668910T3 - Enzimas que degradan la lignina de Macrophomina phaseolina y usos de las mismas - Google Patents
Enzimas que degradan la lignina de Macrophomina phaseolina y usos de las mismas Download PDFInfo
- Publication number
- ES2668910T3 ES2668910T3 ES13829529.0T ES13829529T ES2668910T3 ES 2668910 T3 ES2668910 T3 ES 2668910T3 ES 13829529 T ES13829529 T ES 13829529T ES 2668910 T3 ES2668910 T3 ES 2668910T3
- Authority
- ES
- Spain
- Prior art keywords
- sec
- ident
- lignin
- multicore
- polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229920005610 lignin Polymers 0.000 title claims description 92
- 102000004190 Enzymes Human genes 0.000 title claims description 26
- 108090000790 Enzymes Proteins 0.000 title claims description 26
- 241001495426 Macrophomina phaseolina Species 0.000 title description 54
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 92
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 92
- 239000002157 polynucleotide Substances 0.000 claims abstract description 92
- 108010054320 Lignin peroxidase Proteins 0.000 claims abstract description 18
- 239000002773 nucleotide Substances 0.000 claims abstract description 13
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 13
- 239000000203 mixture Substances 0.000 claims abstract description 11
- 108090000623 proteins and genes Proteins 0.000 claims description 77
- 229920001184 polypeptide Polymers 0.000 claims description 56
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 56
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 56
- 230000015556 catabolic process Effects 0.000 claims description 41
- 238000006731 degradation reaction Methods 0.000 claims description 41
- 241000233866 Fungi Species 0.000 claims description 13
- 210000004027 cell Anatomy 0.000 claims description 9
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 8
- 230000001105 regulatory effect Effects 0.000 claims description 5
- 238000013519 translation Methods 0.000 claims description 5
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 2
- 210000003705 ribosome Anatomy 0.000 claims description 2
- 241001175996 Modiolula phaseolina Species 0.000 claims 1
- 230000000977 initiatory effect Effects 0.000 claims 1
- 230000009261 transgenic effect Effects 0.000 claims 1
- 102000004316 Oxidoreductases Human genes 0.000 description 115
- 108090000854 Oxidoreductases Proteins 0.000 description 115
- 108020004414 DNA Proteins 0.000 description 70
- 102000004169 proteins and genes Human genes 0.000 description 45
- 150000001413 amino acids Chemical class 0.000 description 42
- 238000000246 agarose gel electrophoresis Methods 0.000 description 35
- 238000012408 PCR amplification Methods 0.000 description 34
- 239000003550 marker Substances 0.000 description 34
- -1 aryl glycerol Chemical compound 0.000 description 25
- 150000007523 nucleic acids Chemical group 0.000 description 25
- 230000003647 oxidation Effects 0.000 description 24
- 238000007254 oxidation reaction Methods 0.000 description 24
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 23
- 229910052802 copper Inorganic materials 0.000 description 23
- 239000010949 copper Substances 0.000 description 23
- 108010089792 Hemeproteins Proteins 0.000 description 22
- 102000008015 Hemeproteins Human genes 0.000 description 22
- 150000002989 phenols Chemical class 0.000 description 22
- 108091028043 Nucleic acid sequence Proteins 0.000 description 21
- 108010035722 Chloride peroxidase Proteins 0.000 description 18
- 238000000034 method Methods 0.000 description 15
- 102000003992 Peroxidases Human genes 0.000 description 14
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 13
- 102000039446 nucleic acids Human genes 0.000 description 13
- 108020004707 nucleic acids Proteins 0.000 description 13
- 239000013598 vector Substances 0.000 description 13
- 108020005345 3' Untranslated Regions Proteins 0.000 description 12
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 12
- 108040007629 peroxidase activity proteins Proteins 0.000 description 12
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 11
- 239000002299 complementary DNA Substances 0.000 description 10
- 241000222393 Phanerochaete chrysosporium Species 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 108091026890 Coding region Proteins 0.000 description 7
- 108700026244 Open Reading Frames Proteins 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 239000013612 plasmid Substances 0.000 description 7
- 108091034117 Oligonucleotide Proteins 0.000 description 6
- 101710132589 Peroxidase 2 Proteins 0.000 description 6
- 239000012978 lignocellulosic material Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 5
- 239000013604 expression vector Substances 0.000 description 5
- 239000002028 Biomass Substances 0.000 description 4
- 108010029541 Laccase Proteins 0.000 description 4
- 108010059896 Manganese peroxidase Proteins 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 238000006065 biodegradation reaction Methods 0.000 description 4
- 239000002551 biofuel Substances 0.000 description 4
- JMFRWRFFLBVWSI-NSCUHMNNSA-N coniferol Chemical compound COC1=CC(\C=C\CO)=CC=C1O JMFRWRFFLBVWSI-NSCUHMNNSA-N 0.000 description 4
- 230000000593 degrading effect Effects 0.000 description 4
- 230000006870 function Effects 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 102000003886 Glycoproteins Human genes 0.000 description 3
- 108090000288 Glycoproteins Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229920001131 Pulp (paper) Polymers 0.000 description 3
- 108050009144 Secretory peroxidases Proteins 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 229920002678 cellulose Polymers 0.000 description 3
- 239000001913 cellulose Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 230000001590 oxidative effect Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000004753 textile Substances 0.000 description 3
- 108020004635 Complementary DNA Proteins 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerol Natural products OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 108091092195 Intron Proteins 0.000 description 2
- 241000318373 Macrophomina phaseolina MS6 Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 125000003118 aryl group Chemical class 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229940119526 coniferyl alcohol Drugs 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 230000001419 dependent effect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 108010062085 ligninase Proteins 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 239000011122 softwood Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 239000013603 viral vector Substances 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 1
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- 240000004792 Corchorus capsularis Species 0.000 description 1
- 241000702421 Dependoparvovirus Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 108700020962 Peroxidase Proteins 0.000 description 1
- 241000222385 Phanerochaete Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- BFDMCHRDSYTOLE-UHFFFAOYSA-N SC#N.NC(N)=N.ClC(Cl)Cl.OC1=CC=CC=C1 Chemical compound SC#N.NC(N)=N.ClC(Cl)Cl.OC1=CC=CC=C1 BFDMCHRDSYTOLE-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000008049 TAE buffer Substances 0.000 description 1
- 108010006785 Taq Polymerase Proteins 0.000 description 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 108700009124 Transcription Initiation Site Proteins 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 108030002454 Versatile peroxidases Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- HGEVZDLYZYVYHD-UHFFFAOYSA-N acetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid Chemical compound CC(O)=O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O HGEVZDLYZYVYHD-UHFFFAOYSA-N 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 238000007622 bioinformatic analysis Methods 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000006652 catabolic pathway Effects 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- ATUOYWHBWRKTHZ-UHFFFAOYSA-N dimethylmethane Natural products CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 1
- 229940114124 ferulic acid Drugs 0.000 description 1
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 1
- 235000001785 ferulic acid Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 230000007773 growth pattern Effects 0.000 description 1
- 150000003278 haem Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000002479 lignolytic effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000001668 nucleic acid synthesis Methods 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 235000009048 phenolic acids Nutrition 0.000 description 1
- 229930015704 phenylpropanoid Natural products 0.000 description 1
- 150000002995 phenylpropanoid derivatives Chemical class 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 239000001294 propane Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
- C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
- C12N9/0061—Laccase (1.10.3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
- C12Y111/01—Peroxidases (1.11.1)
- C12Y111/01014—Lignin peroxidase (1.11.1.14)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261683913P | 2012-08-16 | 2012-08-16 | |
| US201261683913P | 2012-08-16 | ||
| PCT/US2013/055199 WO2014028773A2 (en) | 2012-08-16 | 2013-08-15 | Lignin degrading enzymes from macrophomina phaseolina and uses thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2668910T3 true ES2668910T3 (es) | 2018-05-23 |
Family
ID=50101610
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES13829529.0T Active ES2668910T3 (es) | 2012-08-16 | 2013-08-15 | Enzimas que degradan la lignina de Macrophomina phaseolina y usos de las mismas |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US9683221B2 (enExample) |
| EP (1) | EP2885404B1 (enExample) |
| JP (1) | JP6441798B2 (enExample) |
| KR (1) | KR102043356B1 (enExample) |
| CN (1) | CN104919043A (enExample) |
| AU (1) | AU2013302536B2 (enExample) |
| BR (1) | BR112015003087B1 (enExample) |
| DK (1) | DK2885404T3 (enExample) |
| ES (1) | ES2668910T3 (enExample) |
| HK (1) | HK1215047A1 (enExample) |
| HU (1) | HUE038643T2 (enExample) |
| IN (1) | IN2015DN02044A (enExample) |
| MY (1) | MY157478A (enExample) |
| NO (1) | NO2982014T3 (enExample) |
| PL (1) | PL2885404T3 (enExample) |
| PT (1) | PT2885404T (enExample) |
| WO (1) | WO2014028773A2 (enExample) |
| ZA (1) | ZA201501633B (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6474063B2 (ja) * | 2014-08-04 | 2019-02-27 | 国立大学法人山口大学 | 耐熱性微生物の作製方法 |
| KR102398203B1 (ko) * | 2020-11-16 | 2022-05-16 | 전남대학교 산학협력단 | 곰팡이 Podospora anserina S mat+에서 유래된 신규 산소 첨가 효소 및 이의 생산 방법 |
| CN112522117B (zh) * | 2020-12-29 | 2022-06-28 | 中国科学院成都生物研究所 | 一株粪壳菌及其应用 |
| CN117467550A (zh) * | 2023-10-25 | 2024-01-30 | 河北农业大学 | 一株具有降解木质素功能的真菌yz4-1及其分离方法和应用 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5298405A (en) * | 1986-04-30 | 1994-03-29 | Alko Limited | Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production |
| US5232845A (en) * | 1988-11-30 | 1993-08-03 | Idaho Research Foundation Inc. | Bacterial extracellular lignin peroxidase |
| ZA967411B (en) * | 1995-09-01 | 1997-04-16 | Novo Nordisk Biotech Inc | Blue copper oxidase mutants with enhanced activity |
| US6221821B1 (en) | 1998-03-18 | 2001-04-24 | Novozymes A/S Patents | Haloperoxidases with altered pH profiles |
| WO1999047651A1 (en) * | 1998-03-18 | 1999-09-23 | Novo Nordisk A/S | HALOPEROXIDASES WITH ALTERED pH PROFILES |
| US7049485B2 (en) * | 2000-10-20 | 2006-05-23 | Board Of Trustees Of Michigan State University | Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars |
| EP1583512B3 (en) * | 2002-12-12 | 2012-12-05 | R.B.T. (Rakuto Bio Technologies) Ltd. | USE of LIGNIN PEROXIDASE IN SKIN AND HAIR LIGHTENING |
| WO2009005564A2 (en) * | 2007-06-29 | 2009-01-08 | The Regents Of The University Of California | Cellulose- and hemicellulose-degradation enzyme -encoding nucleotide sequences with refined translational kinetics and methods of making same |
| US20090105799A1 (en) | 2007-10-23 | 2009-04-23 | Flowmedica, Inc. | Renal assessment systems and methods |
| US10676751B2 (en) * | 2008-02-29 | 2020-06-09 | The Trustees Of The University Of Pennsylvania | Production and use of plant degrading materials |
| JP5833576B2 (ja) * | 2010-02-25 | 2015-12-16 | ノボザイムス アクティーゼルスカブ | リゾチームの変異体及びそれをコードするポリヌクレオチド |
| JP5692740B2 (ja) * | 2010-03-23 | 2015-04-01 | 学校法人中部大学 | リグニン分解細菌 |
| WO2012027262A1 (en) | 2010-08-23 | 2012-03-01 | Google Inc. | Parallel document mining |
| US20130288310A1 (en) * | 2010-08-23 | 2013-10-31 | Codexis, Inc | Recombinant lignocellulose degradation enzymes for the production of soluble sugars from cellulosic biomass |
-
2013
- 2013-08-15 BR BR112015003087-4A patent/BR112015003087B1/pt active IP Right Grant
- 2013-08-15 US US14/421,739 patent/US9683221B2/en not_active Expired - Fee Related
- 2013-08-15 ES ES13829529.0T patent/ES2668910T3/es active Active
- 2013-08-15 KR KR1020157006681A patent/KR102043356B1/ko not_active Expired - Fee Related
- 2013-08-15 HU HUE13829529A patent/HUE038643T2/hu unknown
- 2013-08-15 WO PCT/US2013/055199 patent/WO2014028773A2/en not_active Ceased
- 2013-08-15 HK HK16102946.3A patent/HK1215047A1/zh unknown
- 2013-08-15 JP JP2015527643A patent/JP6441798B2/ja not_active Expired - Fee Related
- 2013-08-15 CN CN201380054125.6A patent/CN104919043A/zh active Pending
- 2013-08-15 AU AU2013302536A patent/AU2013302536B2/en active Active
- 2013-08-15 DK DK13829529.0T patent/DK2885404T3/en active
- 2013-08-15 EP EP13829529.0A patent/EP2885404B1/en active Active
- 2013-08-15 PL PL13829529T patent/PL2885404T3/pl unknown
- 2013-08-15 MY MYPI2015000386A patent/MY157478A/en unknown
- 2013-08-15 IN IN2044DEN2015 patent/IN2015DN02044A/en unknown
- 2013-08-15 PT PT138295290T patent/PT2885404T/pt unknown
-
2014
- 2014-04-03 NO NO14716271A patent/NO2982014T3/no unknown
-
2015
- 2015-03-10 ZA ZA2015/01633A patent/ZA201501633B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| HUE038643T2 (hu) | 2018-11-28 |
| NO2982014T3 (enExample) | 2017-12-30 |
| BR112015003087A2 (pt) | 2018-09-25 |
| WO2014028773A2 (en) | 2014-02-20 |
| EP2885404A4 (en) | 2016-05-11 |
| KR20150042851A (ko) | 2015-04-21 |
| PL2885404T3 (pl) | 2018-08-31 |
| WO2014028773A3 (en) | 2014-04-17 |
| US9683221B2 (en) | 2017-06-20 |
| ZA201501633B (en) | 2016-01-27 |
| EP2885404B1 (en) | 2018-04-25 |
| PT2885404T (pt) | 2018-05-17 |
| US20150291937A1 (en) | 2015-10-15 |
| AU2013302536A1 (en) | 2015-03-26 |
| BR112015003087B1 (pt) | 2023-03-07 |
| CN104919043A (zh) | 2015-09-16 |
| JP6441798B2 (ja) | 2018-12-19 |
| EP2885404A2 (en) | 2015-06-24 |
| JP2015524673A (ja) | 2015-08-27 |
| AU2013302536B2 (en) | 2017-09-28 |
| HK1215047A1 (zh) | 2016-08-12 |
| KR102043356B1 (ko) | 2019-11-11 |
| IN2015DN02044A (enExample) | 2015-08-14 |
| DK2885404T3 (en) | 2018-05-28 |
| MY157478A (en) | 2016-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Knop et al. | The ligninolytic peroxidases in the genus Pleurotus: divergence in activities, expression, and potential applications | |
| Hildén et al. | The two manganese peroxidases Pr-MnP2 and Pr-MnP3 of Phlebia radiata, a lignin-degrading basidiomycete, are phylogenetically and structurally divergent | |
| Saathoff et al. | Downregulation of cinnamyl-alcohol dehydrogenase in switchgrass by RNA silencing results in enhanced glucose release after cellulase treatment | |
| Takeda et al. | Lignin characterization of rice CONIFERALDEHYDE 5‐HYDROXYLASE loss‐of‐function mutants generated with the CRISPR/Cas9 system | |
| US12275975B2 (en) | Conversion of lignin into bioplastics and lipid fuels | |
| ES2668910T3 (es) | Enzimas que degradan la lignina de Macrophomina phaseolina y usos de las mismas | |
| Bertrand et al. | Functional expression, production, and biochemical characterization of a laccase using yeast surface display technology | |
| Wang et al. | Artificial intelligence modeling and molecular docking to analyze the laccase delignification process of rice straw by Comamonas testosteroni FJ17 | |
| Holzbaur et al. | Molecular biology of lignin peroxidases from Phanerochaete chrysosporium | |
| Aza et al. | Multicopper oxidases with laccase-ferroxidase activity: Classification and study of ferroxidase activity determinants in a member from Heterobasidion annosum sl | |
| CN114641565B (zh) | 重组血红素硫醇盐加氧酶 | |
| Ryu et al. | Molecular characteristics of two laccase from the basidiomycete fungus Polyporus brumalis | |
| US10087425B2 (en) | Chimeric enzymes for conversion of lignin-derived chemicals | |
| Kang et al. | Secretory Production of the Hericium erinaceus Laccase from Saccharomyces cerevisiae | |
| Wang et al. | Molecular characterization and overexpression of mnp6 and vp3 from Pleurotus ostreatus revealed their involvement in biodegradation of cotton stalk lignin | |
| JP2016049019A (ja) | 真正担子菌のリグニン分解能増加方法 | |
| US9441256B2 (en) | Lignases and aldo-keto reductases for conversion of lignin-containing materials to fermentable products | |
| Wang et al. | Construction of SHP-GLOX Straw Lignin Degulation System and Its Application Effect in Rice | |
| Rodríguez Escribano | Directed evolution of extremophilic laccases towards industrial lignin valorisation | |
| Capozzi et al. | Screening of Laccase-Mediator Systems by White-Rot Fungi Laccases for Biocatalytic Benzyl Alcohol Oxidation | |
| Agnestisia et al. | Lignin-Degrading Enzymes From a Pathogenic Canker-Rot Fungus, Inonotus Obliquus Strain IO-B2 (NBRC 113408) | |
| KR100797540B1 (ko) | 겨울우산버섯 라카아제 유전자, 라카아제 효소 및 그생산방법 | |
| 유선화 | Cloning and characterization of lignin degrading enzymes in Polyporus brumalis for biological pretreatment of lignocellulosic biomass | |
| CN116897208A (zh) | 麦角硫因的制造方法 | |
| Kumar et al. | Use of gene specific universal primers for isolation of DNA sequences encoding laccase enzyme from a wild isolate of Schizophyllum commune |