AU2013302536B2 - Lignin degrading enzymes from Macrophomina phaseolina and uses thereof - Google Patents
Lignin degrading enzymes from Macrophomina phaseolina and uses thereof Download PDFInfo
- Publication number
- AU2013302536B2 AU2013302536B2 AU2013302536A AU2013302536A AU2013302536B2 AU 2013302536 B2 AU2013302536 B2 AU 2013302536B2 AU 2013302536 A AU2013302536 A AU 2013302536A AU 2013302536 A AU2013302536 A AU 2013302536A AU 2013302536 B2 AU2013302536 B2 AU 2013302536B2
- Authority
- AU
- Australia
- Prior art keywords
- seq
- polynucleotide
- phaseolina
- group
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229920005610 lignin Polymers 0.000 title claims abstract description 90
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 34
- 102000004190 Enzymes Human genes 0.000 title claims abstract description 33
- 241001495426 Macrophomina phaseolina Species 0.000 title abstract description 113
- 230000000593 degrading effect Effects 0.000 title abstract description 18
- 108091033319 polynucleotide Proteins 0.000 claims abstract description 114
- 102000040430 polynucleotide Human genes 0.000 claims abstract description 114
- 239000002157 polynucleotide Substances 0.000 claims abstract description 114
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 110
- 229920001184 polypeptide Polymers 0.000 claims abstract description 73
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 73
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 73
- 230000015556 catabolic process Effects 0.000 claims abstract description 44
- 238000006731 degradation reaction Methods 0.000 claims abstract description 44
- 239000002773 nucleotide Substances 0.000 claims abstract description 34
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 34
- 241000233866 Fungi Species 0.000 claims abstract description 11
- 230000009261 transgenic effect Effects 0.000 claims abstract description 3
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 13
- 210000004027 cell Anatomy 0.000 claims description 11
- 230000001105 regulatory effect Effects 0.000 claims description 7
- 210000003705 ribosome Anatomy 0.000 claims description 3
- 108700039691 Genetic Promoter Regions Proteins 0.000 claims description 2
- 238000013519 translation Methods 0.000 claims description 2
- 241001175996 Modiolula phaseolina Species 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 15
- 230000000295 complement effect Effects 0.000 abstract description 8
- 238000004519 manufacturing process Methods 0.000 abstract description 8
- 239000002028 Biomass Substances 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 3
- 208000035240 Disease Resistance Diseases 0.000 abstract 1
- 238000003976 plant breeding Methods 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 120
- 102000043368 Multicopper oxidase Human genes 0.000 description 81
- 108700020788 multicopper oxidase Proteins 0.000 description 81
- 102000004169 proteins and genes Human genes 0.000 description 67
- 150000001413 amino acids Chemical class 0.000 description 42
- 230000002441 reversible effect Effects 0.000 description 39
- 108020005345 3' Untranslated Regions Proteins 0.000 description 37
- 239000011543 agarose gel Substances 0.000 description 35
- 238000012408 PCR amplification Methods 0.000 description 34
- 102000004316 Oxidoreductases Human genes 0.000 description 28
- 108090000854 Oxidoreductases Proteins 0.000 description 28
- 108020003589 5' Untranslated Regions Proteins 0.000 description 27
- 108050006227 Haem peroxidases Proteins 0.000 description 26
- -1 cation radicals Chemical class 0.000 description 25
- 150000007523 nucleic acids Chemical group 0.000 description 25
- 230000003647 oxidation Effects 0.000 description 24
- 238000007254 oxidation reaction Methods 0.000 description 24
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 23
- 229910052802 copper Inorganic materials 0.000 description 23
- 239000010949 copper Substances 0.000 description 23
- 150000003278 haem Chemical class 0.000 description 23
- 150000002989 phenols Chemical class 0.000 description 22
- 108010035722 Chloride peroxidase Proteins 0.000 description 18
- 108091028043 Nucleic acid sequence Proteins 0.000 description 16
- 102000003992 Peroxidases Human genes 0.000 description 16
- 102000039446 nucleic acids Human genes 0.000 description 15
- 108020004707 nucleic acids Proteins 0.000 description 15
- 108010054320 Lignin peroxidase Proteins 0.000 description 14
- 239000013598 vector Substances 0.000 description 13
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 12
- 108040007629 peroxidase activity proteins Proteins 0.000 description 12
- 239000000047 product Substances 0.000 description 11
- 239000002299 complementary DNA Substances 0.000 description 10
- 241000222393 Phanerochaete chrysosporium Species 0.000 description 9
- 239000000203 mixture Substances 0.000 description 9
- 108091026890 Coding region Proteins 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 8
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 8
- 108700026244 Open Reading Frames Proteins 0.000 description 7
- 239000013612 plasmid Substances 0.000 description 7
- 101710132589 Peroxidase 2 Proteins 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 239000012978 lignocellulosic material Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 108091034117 Oligonucleotide Proteins 0.000 description 5
- 238000006065 biodegradation reaction Methods 0.000 description 5
- 239000013604 expression vector Substances 0.000 description 5
- 108700020962 Peroxidase Proteins 0.000 description 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 4
- 239000002551 biofuel Substances 0.000 description 4
- JMFRWRFFLBVWSI-NSCUHMNNSA-N coniferol Chemical compound COC1=CC(\C=C\CO)=CC=C1O JMFRWRFFLBVWSI-NSCUHMNNSA-N 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 102000003886 Glycoproteins Human genes 0.000 description 3
- 108090000288 Glycoproteins Proteins 0.000 description 3
- 108091092195 Intron Proteins 0.000 description 3
- 108010059896 Manganese peroxidase Proteins 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 229920001131 Pulp (paper) Polymers 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000001419 dependent effect Effects 0.000 description 3
- 230000001747 exhibiting effect Effects 0.000 description 3
- 230000002538 fungal effect Effects 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 230000001590 oxidative effect Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000003248 secreting effect Effects 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 230000002103 transcriptional effect Effects 0.000 description 3
- NGSWKAQJJWESNS-UHFFFAOYSA-N 4-coumaric acid Chemical compound OC(=O)C=CC1=CC=C(O)C=C1 NGSWKAQJJWESNS-UHFFFAOYSA-N 0.000 description 2
- 108020004635 Complementary DNA Proteins 0.000 description 2
- 241000318373 Macrophomina phaseolina MS6 Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 125000003118 aryl group Chemical class 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 229940119526 coniferyl alcohol Drugs 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 108010062085 ligninase Proteins 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- ODLMAHJVESYWTB-UHFFFAOYSA-N propylbenzene Chemical compound CCCC1=CC=CC=C1 ODLMAHJVESYWTB-UHFFFAOYSA-N 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 239000011122 softwood Substances 0.000 description 2
- 239000004753 textile Substances 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- LZFOPEXOUVTGJS-ONEGZZNKSA-N trans-sinapyl alcohol Chemical compound COC1=CC(\C=C\CO)=CC(OC)=C1O LZFOPEXOUVTGJS-ONEGZZNKSA-N 0.000 description 2
- 230000001131 transforming effect Effects 0.000 description 2
- 239000013603 viral vector Substances 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 1
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 1
- 102100025230 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial Human genes 0.000 description 1
- NGSWKAQJJWESNS-ZZXKWVIFSA-M 4-Hydroxycinnamate Natural products OC1=CC=C(\C=C\C([O-])=O)C=C1 NGSWKAQJJWESNS-ZZXKWVIFSA-M 0.000 description 1
- 101710109578 Acetolactate synthase 1, chloroplastic Proteins 0.000 description 1
- DFYRUELUNQRZTB-UHFFFAOYSA-N Acetovanillone Natural products COC1=CC(C(C)=O)=CC=C1O DFYRUELUNQRZTB-UHFFFAOYSA-N 0.000 description 1
- 229930024421 Adenine Natural products 0.000 description 1
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
- 108010087522 Aeromonas hydrophilia lipase-acyltransferase Proteins 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 229920003043 Cellulose fiber Polymers 0.000 description 1
- 240000004792 Corchorus capsularis Species 0.000 description 1
- 101000916289 Ctenocephalides felis Salivary antigen 1 Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 241000702421 Dependoparvovirus Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 101000629913 Homo sapiens Translocon-associated protein subunit beta Proteins 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 101001098066 Naja melanoleuca Basic phospholipase A2 1 Proteins 0.000 description 1
- 241000222385 Phanerochaete Species 0.000 description 1
- 101710121972 Prolactin-3D1 Proteins 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 239000008049 TAE buffer Substances 0.000 description 1
- 108091036066 Three prime untranslated region Proteins 0.000 description 1
- 108700019146 Transgenes Proteins 0.000 description 1
- 102100026229 Translocon-associated protein subunit beta Human genes 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 108030002454 Versatile peroxidases Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- ZOKIJEBQDZFGMW-PSXMRANNSA-N [(2R)-2-[12-(4-azido-2-nitroanilino)dodecanoyloxy]-3-tetradecanoyloxypropyl] 2-(trimethylazaniumyl)ethyl phosphate Chemical compound CCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCCCCCNc1ccc(cc1[N+]([O-])=O)N=[N+]=[N-] ZOKIJEBQDZFGMW-PSXMRANNSA-N 0.000 description 1
- HGEVZDLYZYVYHD-UHFFFAOYSA-N acetic acid;2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid Chemical compound CC(O)=O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O HGEVZDLYZYVYHD-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 229960000643 adenine Drugs 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 125000003158 alcohol group Chemical group 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 238000007622 bioinformatic analysis Methods 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000006652 catabolic pathway Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- HEDRZPFGACZZDS-UHFFFAOYSA-N chloroform Substances ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 1
- LZFOPEXOUVTGJS-UHFFFAOYSA-N cis-sinapyl alcohol Natural products COC1=CC(C=CCO)=CC(OC)=C1O LZFOPEXOUVTGJS-UHFFFAOYSA-N 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004590 computer program Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 229940104302 cytosine Drugs 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 1
- 229940114124 ferulic acid Drugs 0.000 description 1
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 1
- 235000001785 ferulic acid Nutrition 0.000 description 1
- 230000007773 growth pattern Effects 0.000 description 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-O guanidinium Chemical compound NC(N)=[NH2+] ZRALSGWEFCBTJO-UHFFFAOYSA-O 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000002479 lignolytic effect Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000001668 nucleic acid synthesis Methods 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 229930015763 p-coumaryl alcohol Natural products 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 150000007965 phenolic acids Chemical class 0.000 description 1
- 235000009048 phenolic acids Nutrition 0.000 description 1
- 229930015704 phenylpropanoid Natural products 0.000 description 1
- 150000002995 phenylpropanoid derivatives Chemical class 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 1
- PTNLHDGQWUGONS-UHFFFAOYSA-N trans-p-coumaric alcohol Natural products OCC=CC1=CC=C(O)C=C1 PTNLHDGQWUGONS-UHFFFAOYSA-N 0.000 description 1
- PTNLHDGQWUGONS-OWOJBTEDSA-N trans-p-coumaryl alcohol Chemical compound OC\C=C\C1=CC=C(O)C=C1 PTNLHDGQWUGONS-OWOJBTEDSA-N 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 241000701161 unidentified adenovirus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0055—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10)
- C12N9/0057—Oxidoreductases (1.) acting on diphenols and related substances as donors (1.10) with oxygen as acceptor (1.10.3)
- C12N9/0061—Laccase (1.10.3.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
- C12Y111/01—Peroxidases (1.11.1)
- C12Y111/01014—Lignin peroxidase (1.11.1.14)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261683913P | 2012-08-16 | 2012-08-16 | |
| US61/683,913 | 2012-08-16 | ||
| PCT/US2013/055199 WO2014028773A2 (en) | 2012-08-16 | 2013-08-15 | Lignin degrading enzymes from macrophomina phaseolina and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU2013302536A1 AU2013302536A1 (en) | 2015-03-26 |
| AU2013302536B2 true AU2013302536B2 (en) | 2017-09-28 |
Family
ID=50101610
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2013302536A Active AU2013302536B2 (en) | 2012-08-16 | 2013-08-15 | Lignin degrading enzymes from Macrophomina phaseolina and uses thereof |
Country Status (18)
| Country | Link |
|---|---|
| US (1) | US9683221B2 (enExample) |
| EP (1) | EP2885404B1 (enExample) |
| JP (1) | JP6441798B2 (enExample) |
| KR (1) | KR102043356B1 (enExample) |
| CN (1) | CN104919043A (enExample) |
| AU (1) | AU2013302536B2 (enExample) |
| BR (1) | BR112015003087B1 (enExample) |
| DK (1) | DK2885404T3 (enExample) |
| ES (1) | ES2668910T3 (enExample) |
| HK (1) | HK1215047A1 (enExample) |
| HU (1) | HUE038643T2 (enExample) |
| IN (1) | IN2015DN02044A (enExample) |
| MY (1) | MY157478A (enExample) |
| NO (1) | NO2982014T3 (enExample) |
| PL (1) | PL2885404T3 (enExample) |
| PT (1) | PT2885404T (enExample) |
| WO (1) | WO2014028773A2 (enExample) |
| ZA (1) | ZA201501633B (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6474063B2 (ja) * | 2014-08-04 | 2019-02-27 | 国立大学法人山口大学 | 耐熱性微生物の作製方法 |
| KR102398203B1 (ko) * | 2020-11-16 | 2022-05-16 | 전남대학교 산학협력단 | 곰팡이 Podospora anserina S mat+에서 유래된 신규 산소 첨가 효소 및 이의 생산 방법 |
| CN112522117B (zh) * | 2020-12-29 | 2022-06-28 | 中国科学院成都生物研究所 | 一株粪壳菌及其应用 |
| CN117467550A (zh) * | 2023-10-25 | 2024-01-30 | 河北农业大学 | 一株具有降解木质素功能的真菌yz4-1及其分离方法和应用 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5298405A (en) * | 1986-04-30 | 1994-03-29 | Alko Limited | Enzyme preparations with recombinantly-altered cellulose profiles and methods for their production |
| US5232845A (en) * | 1988-11-30 | 1993-08-03 | Idaho Research Foundation Inc. | Bacterial extracellular lignin peroxidase |
| ZA967411B (en) * | 1995-09-01 | 1997-04-16 | Novo Nordisk Biotech Inc | Blue copper oxidase mutants with enhanced activity |
| US6221821B1 (en) | 1998-03-18 | 2001-04-24 | Novozymes A/S Patents | Haloperoxidases with altered pH profiles |
| WO1999047651A1 (en) * | 1998-03-18 | 1999-09-23 | Novo Nordisk A/S | HALOPEROXIDASES WITH ALTERED pH PROFILES |
| US7049485B2 (en) * | 2000-10-20 | 2006-05-23 | Board Of Trustees Of Michigan State University | Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars |
| EP1583512B3 (en) * | 2002-12-12 | 2012-12-05 | R.B.T. (Rakuto Bio Technologies) Ltd. | USE of LIGNIN PEROXIDASE IN SKIN AND HAIR LIGHTENING |
| WO2009005564A2 (en) * | 2007-06-29 | 2009-01-08 | The Regents Of The University Of California | Cellulose- and hemicellulose-degradation enzyme -encoding nucleotide sequences with refined translational kinetics and methods of making same |
| US20090105799A1 (en) | 2007-10-23 | 2009-04-23 | Flowmedica, Inc. | Renal assessment systems and methods |
| US10676751B2 (en) * | 2008-02-29 | 2020-06-09 | The Trustees Of The University Of Pennsylvania | Production and use of plant degrading materials |
| JP5833576B2 (ja) * | 2010-02-25 | 2015-12-16 | ノボザイムス アクティーゼルスカブ | リゾチームの変異体及びそれをコードするポリヌクレオチド |
| JP5692740B2 (ja) * | 2010-03-23 | 2015-04-01 | 学校法人中部大学 | リグニン分解細菌 |
| WO2012027262A1 (en) | 2010-08-23 | 2012-03-01 | Google Inc. | Parallel document mining |
| US20130288310A1 (en) * | 2010-08-23 | 2013-10-31 | Codexis, Inc | Recombinant lignocellulose degradation enzymes for the production of soluble sugars from cellulosic biomass |
-
2013
- 2013-08-15 BR BR112015003087-4A patent/BR112015003087B1/pt active IP Right Grant
- 2013-08-15 US US14/421,739 patent/US9683221B2/en not_active Expired - Fee Related
- 2013-08-15 ES ES13829529.0T patent/ES2668910T3/es active Active
- 2013-08-15 KR KR1020157006681A patent/KR102043356B1/ko not_active Expired - Fee Related
- 2013-08-15 HU HUE13829529A patent/HUE038643T2/hu unknown
- 2013-08-15 WO PCT/US2013/055199 patent/WO2014028773A2/en not_active Ceased
- 2013-08-15 HK HK16102946.3A patent/HK1215047A1/zh unknown
- 2013-08-15 JP JP2015527643A patent/JP6441798B2/ja not_active Expired - Fee Related
- 2013-08-15 CN CN201380054125.6A patent/CN104919043A/zh active Pending
- 2013-08-15 AU AU2013302536A patent/AU2013302536B2/en active Active
- 2013-08-15 DK DK13829529.0T patent/DK2885404T3/en active
- 2013-08-15 EP EP13829529.0A patent/EP2885404B1/en active Active
- 2013-08-15 PL PL13829529T patent/PL2885404T3/pl unknown
- 2013-08-15 MY MYPI2015000386A patent/MY157478A/en unknown
- 2013-08-15 IN IN2044DEN2015 patent/IN2015DN02044A/en unknown
- 2013-08-15 PT PT138295290T patent/PT2885404T/pt unknown
-
2014
- 2014-04-03 NO NO14716271A patent/NO2982014T3/no unknown
-
2015
- 2015-03-10 ZA ZA2015/01633A patent/ZA201501633B/en unknown
Non-Patent Citations (1)
| Title |
|---|
| SCHALCH H ET AL, MOLECULAR CLONING AND SEQUENCES OF LIGNIN PEROXIDASE GENES OF PHANEROCHAETE-CHRYSOSPORIUM, MOLECULAR AND CELLULAR BIOLOGY, VOL. 9, NR. 6, PAGE(S) 2743-2747. * |
Also Published As
| Publication number | Publication date |
|---|---|
| HUE038643T2 (hu) | 2018-11-28 |
| NO2982014T3 (enExample) | 2017-12-30 |
| BR112015003087A2 (pt) | 2018-09-25 |
| WO2014028773A2 (en) | 2014-02-20 |
| EP2885404A4 (en) | 2016-05-11 |
| KR20150042851A (ko) | 2015-04-21 |
| PL2885404T3 (pl) | 2018-08-31 |
| ES2668910T3 (es) | 2018-05-23 |
| WO2014028773A3 (en) | 2014-04-17 |
| US9683221B2 (en) | 2017-06-20 |
| ZA201501633B (en) | 2016-01-27 |
| EP2885404B1 (en) | 2018-04-25 |
| PT2885404T (pt) | 2018-05-17 |
| US20150291937A1 (en) | 2015-10-15 |
| AU2013302536A1 (en) | 2015-03-26 |
| BR112015003087B1 (pt) | 2023-03-07 |
| CN104919043A (zh) | 2015-09-16 |
| JP6441798B2 (ja) | 2018-12-19 |
| EP2885404A2 (en) | 2015-06-24 |
| JP2015524673A (ja) | 2015-08-27 |
| HK1215047A1 (zh) | 2016-08-12 |
| KR102043356B1 (ko) | 2019-11-11 |
| IN2015DN02044A (enExample) | 2015-08-14 |
| DK2885404T3 (en) | 2018-05-28 |
| MY157478A (en) | 2016-06-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Hildén et al. | The two manganese peroxidases Pr-MnP2 and Pr-MnP3 of Phlebia radiata, a lignin-degrading basidiomycete, are phylogenetically and structurally divergent | |
| AU2013302536B2 (en) | Lignin degrading enzymes from Macrophomina phaseolina and uses thereof | |
| Meng et al. | The transcription factor FvHmg1 negatively regulates fruiting body development in winter mushroom Flammulina velutipes | |
| Wang et al. | Transcriptomics analysis reveals the high biodegradation efficiency of white-rot fungus Phanerochaete sordida YK-624 on native lignin | |
| Wang et al. | Artificial intelligence modeling and molecular docking to analyze the laccase delignification process of rice straw by Comamonas testosteroni FJ17 | |
| WO2010117843A2 (en) | Termite enzymes and uses thereof for in vitro conversion of lignin-containing materials to fermentable products | |
| CN112250740B (zh) | 葡萄糖转运蛋白及其在提高有机酸生产中的应用 | |
| JP2009027999A (ja) | シス−アコニット酸デカルボキシラーゼをコードするｄｎａ、シス−アコニット酸デカルボキシラーゼの製造方法、及び、イタコン酸の製造方法 | |
| CN120026012B (zh) | 角蛋白酶突变体、其编码基因和表达载体及其应用 | |
| Hilden et al. | Expression on wood, molecular cloning and characterization of three lignin peroxidase (LiP) encoding genes of the white rot fungus Phlebia radiata | |
| CN102719417A (zh) | 一种耐高温阿拉伯呋喃糖苷酶Abf51B8及其基因和应用 | |
| Tang et al. | Cloning and expression of cellulase gene EG1 from Rhizopus stolonifer var. reflexus TP-02 in Escherichia coli | |
| US10087425B2 (en) | Chimeric enzymes for conversion of lignin-derived chemicals | |
| EP2970869A2 (en) | Low-phosphate repressible promoter | |
| KR101262250B1 (ko) | 라카아제를 생산하는 콜트리시아 퍼렌니스, 그 균주 유래 라카아제 효소 및 그 효소 생산 방법 | |
| US20150184211A1 (en) | Novel lignases and aldo-keto reductases for conversion of lignin-containing materials to fermentable products | |
| JP2016049019A (ja) | 真正担子菌のリグニン分解能増加方法 | |
| CN103233025A (zh) | 一种用毕赤酵母工程菌联合降解植物的木质素的方法 | |
| KR100797540B1 (ko) | 겨울우산버섯 라카아제 유전자, 라카아제 효소 및 그생산방법 | |
| JP5567360B2 (ja) | リグニンペルオキシダーゼ遺伝子およびマンガンペルオキシダーゼ遺伝子を検出するためのオリゴヌクレオチド並びにこれを用いたリグニンペルオキシダーゼ遺伝子およびマンガンペルオキシダーゼ遺伝子のスクリーニング方法 | |
| AU2013302535B2 (en) | Pectin degrading enzymes from Macrophomina phaseolina and uses thereof | |
| KR20090092517A (ko) | 내분비장애 물질의 생분해능이 향상된 백색부후균형질전환체 및 이의 제조방법 | |
| Yun-hang et al. | Isolation and identification of lignin decomposing bacteria and cloning of lip gene in termite gut | |
| 유선화 | Cloning and characterization of lignin degrading enzymes in Polyporus brumalis for biological pretreatment of lignocellulosic biomass | |
| WO2016058171A1 (en) | Method for directional cloning |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGA | Letters patent sealed or granted (standard patent) |