ES2654188T3 - Procedimiento universal para determinar los valores de umbral del ciclo de PCR en tiempo real - Google Patents
Procedimiento universal para determinar los valores de umbral del ciclo de PCR en tiempo real Download PDFInfo
- Publication number
- ES2654188T3 ES2654188T3 ES13779153.9T ES13779153T ES2654188T3 ES 2654188 T3 ES2654188 T3 ES 2654188T3 ES 13779153 T ES13779153 T ES 13779153T ES 2654188 T3 ES2654188 T3 ES 2654188T3
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- ES
- Spain
- Prior art keywords
- function
- point
- pcr
- calculate
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- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F17/00—Digital computing or data processing equipment or methods, specially adapted for specific functions
- G06F17/10—Complex mathematical operations
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- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F17/00—Digital computing or data processing equipment or methods, specially adapted for specific functions
- G06F17/10—Complex mathematical operations
- G06F17/17—Function evaluation by approximation methods, e.g. inter- or extrapolation, smoothing, least mean square method
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- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B40/00—ICT specially adapted for biostatistics; ICT specially adapted for bioinformatics-related machine learning or data mining, e.g. knowledge discovery or pattern finding
- G16B40/10—Signal processing, e.g. from mass spectrometry [MS] or from PCR
Landscapes
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Theoretical Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Medical Informatics (AREA)
- Data Mining & Analysis (AREA)
- General Physics & Mathematics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Evolutionary Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Mathematical Physics (AREA)
- General Engineering & Computer Science (AREA)
- Databases & Information Systems (AREA)
- Software Systems (AREA)
- Mathematical Analysis (AREA)
- Mathematical Optimization (AREA)
- Pure & Applied Mathematics (AREA)
- Computational Mathematics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Algebra (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioethics (AREA)
- Public Health (AREA)
- Evolutionary Computation (AREA)
- Epidemiology (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/633,813 US10176293B2 (en) | 2012-10-02 | 2012-10-02 | Universal method to determine real-time PCR cycle threshold values |
| US201213633813 | 2012-10-02 | ||
| PCT/EP2013/070413 WO2014053467A1 (en) | 2012-10-02 | 2013-10-01 | Universal method to determine real-time pcr cycle threshold values |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2654188T3 true ES2654188T3 (es) | 2018-02-12 |
Family
ID=49385222
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES13779153.9T Active ES2654188T3 (es) | 2012-10-02 | 2013-10-01 | Procedimiento universal para determinar los valores de umbral del ciclo de PCR en tiempo real |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10176293B2 (enExample) |
| EP (1) | EP2904110B1 (enExample) |
| JP (1) | JP6280124B2 (enExample) |
| CN (1) | CN104662174B (enExample) |
| CA (1) | CA2883825A1 (enExample) |
| ES (1) | ES2654188T3 (enExample) |
| WO (1) | WO2014053467A1 (enExample) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10993418B2 (en) | 2012-08-13 | 2021-05-04 | Life Genetics Lab, Llc | Method for measuring tumor burden in patient derived xenograft (PDX) mice |
| US9957557B2 (en) | 2012-08-13 | 2018-05-01 | Life Genetics Lab, Llc | Development of a highly sensitive quantification system for assessing DNA degradation and quality in forensic samples |
| CN104237291B (zh) * | 2014-08-14 | 2017-03-01 | 超威电源有限公司 | 一种评价胶体电解质凝胶时间的方法 |
| EP3770272B8 (en) * | 2014-08-27 | 2021-10-27 | Roche Diagnostics GmbH | An analysis method and system for analyzing a nucleic acid amplification reaction |
| CN107083426A (zh) * | 2017-03-30 | 2017-08-22 | 杭州晶格科学仪器有限公司 | 一种荧光定量检测方法 |
| KR102420644B1 (ko) * | 2017-09-28 | 2022-07-13 | 주식회사 씨젠 | 시료 내 타겟 분석물을 분석하는 방법 및 장치 |
| CN110231254A (zh) * | 2019-06-12 | 2019-09-13 | 刘华云 | 一种用于接触角测量仪的内置计算方法 |
| DE102020202361A1 (de) | 2020-02-25 | 2021-08-26 | Robert Bosch Gesellschaft mit beschränkter Haftung | Verfahren und Vorrichtung zur Auswertung einer qPCR-Kurve |
| DE102020202358B4 (de) | 2020-02-25 | 2023-09-21 | Robert Bosch Gesellschaft mit beschränkter Haftung | Verfahren und Vorrichtung zur Durchführung eines qPCR-Verfahrens |
| DE102020202360B4 (de) | 2020-02-25 | 2024-03-21 | Robert Bosch Gesellschaft mit beschränkter Haftung | Verfahren und Vorrichtung zur Durchführung eines qPCR-Verfahrens |
| DE102020202363A1 (de) | 2020-02-25 | 2021-08-26 | Robert Bosch Gesellschaft mit beschränkter Haftung | Verfahren und Vorrichtung zur Durchführung eines qPCR-Verfahrens |
| CN111593098B (zh) * | 2020-05-29 | 2021-09-21 | 成都瀚辰光翼科技有限责任公司 | 一种qpcr实时荧光数据定量分析的方法 |
| DE102020116178A1 (de) | 2020-06-18 | 2021-12-23 | Analytik Jena Gmbh | Verfahren zum Erkennen einer Amplifikationsphase in einer Amplifikation |
| CN112782395B (zh) * | 2020-12-30 | 2021-11-09 | 北京中科生仪科技有限公司 | 一种pcr检测仪及其方法 |
| CN115312123A (zh) * | 2021-05-07 | 2022-11-08 | 台达电子工业股份有限公司 | 数据预筛方法及其数据预筛装置 |
| CN113851189B (zh) * | 2021-09-24 | 2022-09-23 | 中元汇吉生物技术股份有限公司 | 一种自适应检测pcr荧光基线的方法、装置及其设备 |
| CN114058683B (zh) * | 2021-11-17 | 2022-09-30 | 杭州优思达生物技术有限公司 | 一种核酸扩增结果判定方法 |
| CN114065120B (zh) * | 2022-01-14 | 2022-04-19 | 深圳市刚竹医疗科技有限公司 | 测定pcr的直线-s形生长曲线的循环阈值的方法 |
| WO2024019471A1 (ko) * | 2022-07-18 | 2024-01-25 | 아주대학교산학협력단 | 지수함수를 이용한 생존곡선 생성 시스템 및 그 방법 |
| CN116189784A (zh) * | 2023-01-18 | 2023-05-30 | 杭州奥盛仪器有限公司 | 核酸扩增曲线分析方法、装置、设备及存储介质 |
| AU2024267362A1 (en) * | 2023-05-05 | 2025-11-13 | Gen-Probe Incorporated | Method and system for improving specificity of analyte detection using real-time nucleic acid amplification |
| US20250110640A1 (en) * | 2023-10-03 | 2025-04-03 | Yang Yang | Method and system to perform storage capacity planning in hyper-converged infrastructure environment |
| WO2025143555A1 (ko) * | 2023-12-26 | 2025-07-03 | 주식회사 씨젠 | 샘플 내 타겟 분석물질을 검출하는 방법 |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5373457A (en) | 1993-03-29 | 1994-12-13 | Motorola, Inc. | Method for deriving a piecewise linear model |
| AU726501B2 (en) | 1996-06-04 | 2000-11-09 | University Of Utah Research Foundation | Monitoring hybridization during PCR |
| ATE390491T1 (de) | 1996-06-04 | 2008-04-15 | Univ Utah Res Found | Behälter zum durchführen und beobachten biologischer prozesse |
| GB9809839D0 (en) | 1998-05-09 | 1998-07-08 | Glaxo Group Ltd | Antibody |
| GB9821989D0 (en) | 1998-10-08 | 1998-12-02 | Hybaid Ltd | Detection of nucleic acid polymorphism |
| US6303305B1 (en) | 1999-03-30 | 2001-10-16 | Roche Diagnostics, Gmbh | Method for quantification of an analyte |
| US6783934B1 (en) | 2000-05-01 | 2004-08-31 | Cepheid, Inc. | Methods for quantitative analysis of nucleic acid amplification reaction |
| US7043461B2 (en) * | 2001-01-19 | 2006-05-09 | Genalytics, Inc. | Process and system for developing a predictive model |
| US7228237B2 (en) | 2002-02-07 | 2007-06-05 | Applera Corporation | Automatic threshold setting and baseline determination for real-time PCR |
| US7788039B2 (en) | 2003-09-25 | 2010-08-31 | Roche Molecular Systems, Inc. | Quantitation of nucleic acids using growth curves |
| US20060009916A1 (en) | 2004-07-06 | 2006-01-12 | Xitong Li | Quantitative PCR data analysis system (QDAS) |
| US7680604B2 (en) | 2005-03-11 | 2010-03-16 | Roche Molecular Systems, Inc. | PCR elbow determination by rotational transform after zero slope alignment |
| CN101292245B (zh) | 2005-05-13 | 2013-04-17 | 生物辐射实验室股份有限公司 | 识别统计线性数据的设备和方法 |
| US7991561B2 (en) | 2005-09-29 | 2011-08-02 | Roche Molecular Systems, Inc. | Ct determination by cluster analysis with variable cluster endpoint |
| US7668663B2 (en) | 2005-12-20 | 2010-02-23 | Roche Molecular Systems, Inc. | Levenberg-Marquardt outlier spike removal method |
| US7991562B2 (en) | 2005-12-20 | 2011-08-02 | Roche Molecular Systems, Inc. | PCR elbow determination using curvature analysis of a double sigmoid |
| US7844403B2 (en) | 2005-12-20 | 2010-11-30 | Roche Molecular Systems, Inc. | Temperature step correction with double sigmoid Levenberg-Marquardt and robust linear regression |
| EP1804172B1 (en) | 2005-12-20 | 2021-08-11 | Roche Diagnostics GmbH | PCR elbow determination using curvature analysis of a double sigmoid |
| US7680868B2 (en) | 2005-12-20 | 2010-03-16 | Roche Molecular Systems, Inc. | PCR elbow determination by use of a double sigmoid function curve fit with the Levenburg-Marquardt algorithm and normalization |
| US20090119020A1 (en) | 2007-09-25 | 2009-05-07 | Roche Molecular Systems, Inc. | Pcr elbow determination using quadratic test for curvature analysis of a double sigmoid |
| US8374795B2 (en) | 2008-05-13 | 2013-02-12 | Roche Molecular Systems, Inc. | Systems and methods for step discontinuity removal in real-time PCR fluorescence data |
| US8219324B2 (en) * | 2008-09-12 | 2012-07-10 | Roche Molecular Systems, Inc. | Real-time PCR elbow calling by equation-less algorithm |
| US8219366B2 (en) | 2009-08-26 | 2012-07-10 | Roche Molecular Sytems, Inc. | Determination of elbow values for PCR for parabolic shaped curves |
| US20130189702A1 (en) | 2010-04-21 | 2013-07-25 | Siemens Healthcare Diagnostics Inc. | Curve Processor Algorithm for the Quality Control of (RT-) qPCR Curves |
-
2012
- 2012-10-02 US US13/633,813 patent/US10176293B2/en active Active
-
2013
- 2013-10-01 WO PCT/EP2013/070413 patent/WO2014053467A1/en not_active Ceased
- 2013-10-01 CN CN201380051030.9A patent/CN104662174B/zh active Active
- 2013-10-01 EP EP13779153.9A patent/EP2904110B1/en active Active
- 2013-10-01 CA CA2883825A patent/CA2883825A1/en not_active Abandoned
- 2013-10-01 ES ES13779153.9T patent/ES2654188T3/es active Active
- 2013-10-01 JP JP2015534977A patent/JP6280124B2/ja active Active
-
2018
- 2018-11-28 US US16/203,070 patent/US11615863B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CA2883825A1 (en) | 2014-04-10 |
| US20190095576A1 (en) | 2019-03-28 |
| JP6280124B2 (ja) | 2018-02-14 |
| WO2014053467A1 (en) | 2014-04-10 |
| JP2016500863A (ja) | 2016-01-14 |
| CN104662174B (zh) | 2018-03-02 |
| US20140095080A1 (en) | 2014-04-03 |
| CN104662174A (zh) | 2015-05-27 |
| US10176293B2 (en) | 2019-01-08 |
| EP2904110B1 (en) | 2017-08-30 |
| WO2014053467A9 (en) | 2014-08-28 |
| EP2904110A1 (en) | 2015-08-12 |
| US11615863B2 (en) | 2023-03-28 |
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