ES2628677R1 - Método y dispositivo para detección de bacterias enteras - Google Patents

Método y dispositivo para detección de bacterias enteras Download PDF

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Publication number
ES2628677R1
ES2628677R1 ES201690063A ES201690063A ES2628677R1 ES 2628677 R1 ES2628677 R1 ES 2628677R1 ES 201690063 A ES201690063 A ES 201690063A ES 201690063 A ES201690063 A ES 201690063A ES 2628677 R1 ES2628677 R1 ES 2628677R1
Authority
ES
Spain
Prior art keywords
whole bacteria
detection
devices
water sample
bacteria
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES201690063A
Other languages
English (en)
Other versions
ES2628677A2 (es
ES2628677B2 (es
Inventor
Klariska GOVINDASAMY
Suretha POTGIETER
Kevin LAND
Shavon KUMAR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Council for Scientific and Industrial Research CSIR
Original Assignee
Council for Scientific and Industrial Research CSIR
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Council for Scientific and Industrial Research CSIR filed Critical Council for Scientific and Industrial Research CSIR
Publication of ES2628677A2 publication Critical patent/ES2628677A2/es
Publication of ES2628677R1 publication Critical patent/ES2628677R1/es
Application granted granted Critical
Publication of ES2628677B2 publication Critical patent/ES2628677B2/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody

Abstract

Método y dispositivo para detección de bacterias enteras.#La presente invención proporciona dispositivos de flujo lateral, dispositivos microfluídicos y métodos para detectar bacterias enteras en una muestra de agua. Los dispositivos y métodos proporcionan la detección rápida in situ de bacterias enteras en una muestra de agua.
ES201690063A 2014-05-30 2015-05-29 Método y dispositivo para detección de bacterias enteras Active ES2628677B2 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ZA2014/03971 2014-05-30
ZA201403971 2014-05-30
PCT/IB2015/054060 WO2015181790A1 (en) 2014-05-30 2015-05-29 Method and device for detection of whole organism bacteria

Publications (3)

Publication Number Publication Date
ES2628677A2 ES2628677A2 (es) 2017-08-03
ES2628677R1 true ES2628677R1 (es) 2017-11-29
ES2628677B2 ES2628677B2 (es) 2018-08-09

Family

ID=53499046

Family Applications (2)

Application Number Title Priority Date Filing Date
ES201690063A Active ES2628677B2 (es) 2014-05-30 2015-05-29 Método y dispositivo para detección de bacterias enteras
ES201830423A Pending ES2696757R1 (es) 2014-05-30 2015-05-29 Método y dispositivo para detección de bacterias enteras

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES201830423A Pending ES2696757R1 (es) 2014-05-30 2015-05-29 Método y dispositivo para detección de bacterias enteras

Country Status (4)

Country Link
ES (2) ES2628677B2 (es)
GB (1) GB2549819B (es)
WO (1) WO2015181790A1 (es)
ZA (1) ZA201607845B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106526176A (zh) * 2016-12-05 2017-03-22 无锡艾科瑞思产品设计与研究有限公司 一种多功能荧光免疫层析快速定量检测卡
CN110806477A (zh) * 2018-08-06 2020-02-18 国家纳米科学中心 一种病原菌检测试纸条、传感器及其应用
CN115232779A (zh) * 2022-07-18 2022-10-25 浙江省农业科学院 一种利用次氯酸钠诱导肠炎沙门氏菌进入vbnc态的方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004065010A2 (en) * 2003-01-21 2004-08-05 Micronics Inc. Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing
WO2013105090A1 (en) * 2012-01-11 2013-07-18 Aptateck Bio Ltd. A versatile lateral flow strip device
US20130236899A1 (en) * 2012-03-09 2013-09-12 Invisible Sentinel, Inc. Methods And Compositions For Detecting Multiple Analytes With A Single Signal
WO2014035270A1 (en) * 2012-08-31 2014-03-06 Wrocławskie Centrum Badań Eit + Spółka Z Organiczona A method of identifying gram-negative bacteria using the maldi-tof ms method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE479096T1 (de) * 2004-07-29 2010-09-15 Relia Diagnostic Systems Llc Querstromsystem und assay
TR201818881T4 (tr) * 2011-11-21 2019-01-21 Abay Sa Sinyal Amplifikasyonu İmmünodeneyleri

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004065010A2 (en) * 2003-01-21 2004-08-05 Micronics Inc. Method and system for microfluidic manipulation, amplification and analysis of fluids, for example, bacteria assays and antiglobulin testing
WO2013105090A1 (en) * 2012-01-11 2013-07-18 Aptateck Bio Ltd. A versatile lateral flow strip device
US20130236899A1 (en) * 2012-03-09 2013-09-12 Invisible Sentinel, Inc. Methods And Compositions For Detecting Multiple Analytes With A Single Signal
WO2014035270A1 (en) * 2012-08-31 2014-03-06 Wrocławskie Centrum Badań Eit + Spółka Z Organiczona A method of identifying gram-negative bacteria using the maldi-tof ms method

Also Published As

Publication number Publication date
GB201621723D0 (en) 2017-02-01
ES2696757R1 (es) 2019-01-21
ES2628677A2 (es) 2017-08-03
ES2696757A2 (es) 2019-01-17
GB2549819A (en) 2017-11-01
ES2628677B2 (es) 2018-08-09
GB2549819B (en) 2019-04-17
WO2015181790A1 (en) 2015-12-03
ZA201607845B (en) 2019-07-31

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