ES2538100T3 - Procedimiento para la realización y evaluación de ensayos de mezclado y medición para la medición de cinéticas de reacción así como de concentraciones y afinidades de analitos en formato múltiplex - Google Patents

Procedimiento para la realización y evaluación de ensayos de mezclado y medición para la medición de cinéticas de reacción así como de concentraciones y afinidades de analitos en formato múltiplex Download PDF

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Publication number
ES2538100T3
ES2538100T3 ES08761034.1T ES08761034T ES2538100T3 ES 2538100 T3 ES2538100 T3 ES 2538100T3 ES 08761034 T ES08761034 T ES 08761034T ES 2538100 T3 ES2538100 T3 ES 2538100T3
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ES
Spain
Prior art keywords
fluorescence
microparticles
carrier
populations
different
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08761034.1T
Other languages
English (en)
Spanish (es)
Inventor
Werner Lehmann
Alexander BÖHM
Kai Grossmann
Rico Hiemann
Jörg NITSCHKE
Stefan RÖDIGER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Attomol Molekulare Diagnostika GmbH
Original Assignee
Attomol Molekulare Diagnostika GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE102007031137A external-priority patent/DE102007031137A1/de
Application filed by Attomol Molekulare Diagnostika GmbH filed Critical Attomol Molekulare Diagnostika GmbH
Application granted granted Critical
Publication of ES2538100T3 publication Critical patent/ES2538100T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
ES08761034.1T 2007-06-13 2008-06-13 Procedimiento para la realización y evaluación de ensayos de mezclado y medición para la medición de cinéticas de reacción así como de concentraciones y afinidades de analitos en formato múltiplex Active ES2538100T3 (es)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
DE102007027779 2007-06-13
DE102007027779 2007-06-13
DE102007031137 2007-06-29
DE102007031137A DE102007031137A1 (de) 2007-06-13 2007-06-29 Verfahren und Sonden/Primärsystem zum "real time" Nachweis eines Nukleinsäuretargets
DE102008008485 2008-02-08
DE102008008485 2008-02-08
PCT/EP2008/057515 WO2008152145A1 (de) 2007-06-13 2008-06-13 Verfahren zur durchführung und auswertung von mix & measure assays für die messung von reaktionskinetiken sowie von konzentrationen und affinitäten von analyten im multiplexformat

Publications (1)

Publication Number Publication Date
ES2538100T3 true ES2538100T3 (es) 2015-06-17

Family

ID=40129269

Family Applications (1)

Application Number Title Priority Date Filing Date
ES08761034.1T Active ES2538100T3 (es) 2007-06-13 2008-06-13 Procedimiento para la realización y evaluación de ensayos de mezclado y medición para la medición de cinéticas de reacción así como de concentraciones y afinidades de analitos en formato múltiplex

Country Status (4)

Country Link
US (1) US20100203572A1 (en10)
EP (2) EP2167964B1 (en10)
ES (1) ES2538100T3 (en10)
WO (2) WO2008152144A1 (en10)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9587270B2 (en) 2009-06-29 2017-03-07 Luminex Corporation Chimeric primers with hairpin conformations and methods of using same
EP2483425B1 (en) * 2009-09-28 2016-08-24 Igor Kutyavin Methods and compositions for detection of nucleic acids based on stabilized oligonucleotide probe complexes
SI23321A (sl) * 2010-03-03 2011-09-30 Inštitut za fizikalno biologijo d.o.o. Izboljĺ ano zbiranje fluorescentne svetlobe iz vzorca med pomnoĺ˝evanjem v rt-pcr
GB201007867D0 (en) * 2010-05-11 2010-06-23 Enigma Diagnostics Ltd Signalling system
US20120034703A1 (en) * 2010-08-06 2012-02-09 Affymetrix, Inc. Devices, Systems and Methods for Processing of Magnetic Particles
US9329127B2 (en) 2011-04-28 2016-05-03 Bio-Rad Laboratories, Inc. Fluorescence scanning head with multiband detection
CN108192952A (zh) 2011-09-29 2018-06-22 露美内克丝公司 水解探针
CN113376146A (zh) * 2020-02-25 2021-09-10 上海交通大学 适于生物分子多重检测的检测颗粒及其制备方法与应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0566751B1 (en) * 1992-03-23 1996-01-10 F. Hoffmann-La Roche Ag DNA detection method
EP0892808B1 (en) * 1996-04-12 2008-05-14 PHRI Properties, Inc. Detection probes, kits and assays
PT912766E (pt) * 1996-06-04 2009-07-16 Univ Utah Res Found Monitorização da hibridização durante a pcr
US6327410B1 (en) * 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
GB9812768D0 (en) * 1998-06-13 1998-08-12 Zeneca Ltd Methods
US6514750B2 (en) * 2001-07-03 2003-02-04 Pe Corporation (Ny) PCR sample handling device
AUPS076802A0 (en) * 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Selective nucleic acid amplification
JP4457001B2 (ja) * 2002-05-31 2010-04-28 セクレタリー・デパートメント・オブ・アトミック・エナジー ドナー／アクセプター部分が相補鎖上となる標的核酸検出のｍｅｔ／ｆｒｅｔに基づく方法
WO2004027093A1 (en) * 2002-09-19 2004-04-01 The Chancellor, Master And Scholars Of The University Of Oxford Molecular arrays and single molecule detection
US20070059693A1 (en) * 2003-01-02 2007-03-15 University Of Rochester Hybridization-based biosensor containing hairpin probes and use thereof
CA2545252A1 (en) * 2003-11-06 2005-05-26 University Of Nevada, Reno Improved methods for detecting and measuring specific nucleic acid sequences
WO2006122208A1 (en) * 2005-05-11 2006-11-16 Stratagene California Snapback oligonucleotide probe

Also Published As

Publication number Publication date
EP2167964B1 (de) 2015-03-11
US20100203572A1 (en) 2010-08-12
WO2008152144A1 (de) 2008-12-18
EP2167685B1 (de) 2014-12-17
EP2167964A1 (de) 2010-03-31
WO2008152145A1 (de) 2008-12-18
EP2167685A1 (de) 2010-03-31

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