ES2536734T3 - Método para detectar microorganismos del género Kingella - Google Patents

Método para detectar microorganismos del género Kingella Download PDF

Info

Publication number
ES2536734T3
ES2536734T3 ES09783197.8T ES09783197T ES2536734T3 ES 2536734 T3 ES2536734 T3 ES 2536734T3 ES 09783197 T ES09783197 T ES 09783197T ES 2536734 T3 ES2536734 T3 ES 2536734T3
Authority
ES
Spain
Prior art keywords
detect microorganisms
kingella
genus
microorganism
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES09783197.8T
Other languages
English (en)
Inventor
Stéphane BONACORSI
Philippe Bidet
Edouard Bingen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Assistance Publique Hopitaux de Paris APHP
Universite Paris Diderot Paris 7
Original Assignee
Assistance Publique Hopitaux de Paris APHP
Universite Paris Diderot Paris 7
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Assistance Publique Hopitaux de Paris APHP, Universite Paris Diderot Paris 7 filed Critical Assistance Publique Hopitaux de Paris APHP
Application granted granted Critical
Publication of ES2536734T3 publication Critical patent/ES2536734T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un método para detectar la presencia de un microorganismo de Kingella kingae en una muestra biológica de un paciente, que comprende las etapas de a. Llevar a cabo una amplificación PCR del ADN en dicha muestra con el par de cebadores de SEC ID Nº: 2 y SEC ID Nº: 3 b. Detectar la presencia o la ausencia de un ácido nucleico amplificado, en el que la presencia de un ácido nucleico amplificado supone la presencia de un microorganismo de Kingella kingae en dicha muestra biológica.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
imagen8

Claims (1)

  1. imagen1
ES09783197.8T 2008-09-19 2009-09-18 Método para detectar microorganismos del género Kingella Active ES2536734T3 (es)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP08164748 2008-09-19
EP08164748 2008-09-19
EP09163319 2009-06-19
EP09163319 2009-06-19
PCT/EP2009/062142 WO2010031851A1 (en) 2008-09-19 2009-09-18 Method for detecting microorganisms of kingella genus

Publications (1)

Publication Number Publication Date
ES2536734T3 true ES2536734T3 (es) 2015-05-28

Family

ID=41381606

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09783197.8T Active ES2536734T3 (es) 2008-09-19 2009-09-18 Método para detectar microorganismos del género Kingella

Country Status (5)

Country Link
US (1) US8735069B2 (es)
EP (1) EP2342358B1 (es)
JP (1) JP5688021B2 (es)
ES (1) ES2536734T3 (es)
WO (1) WO2010031851A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116072241B (zh) * 2023-02-06 2025-07-22 杭州杰毅生物技术有限公司 一种预测肺炎克雷伯菌对环丙沙星敏感性的系统及方法

Also Published As

Publication number Publication date
US20110229900A1 (en) 2011-09-22
US8735069B2 (en) 2014-05-27
JP5688021B2 (ja) 2015-03-25
EP2342358B1 (en) 2015-02-18
EP2342358A1 (en) 2011-07-13
JP2012502644A (ja) 2012-02-02
WO2010031851A1 (en) 2010-03-25

Similar Documents

Publication Publication Date Title
ES2556595T3 (es) Métodos de amplificación dependiente de helicasa y detección de polinucleótidos
JP7104730B2 (ja) 連続的な増幅反応のための方法および装置
ES2522912T3 (es) Métodos para el enriquecimiento de secuencias basadas en solución y análisis de regiones genómicas
EA201170933A1 (ru) Анализ нуклеиновых кислот, полученных из единичных клеток
MX2022012074A (es) Métodos para la detección de bajos niveles del virus de la covid-19.
WO2008079463A3 (en) Biological organism identification product and methods
PE20110480A1 (es) Microorganismos y vacunas dependientes de replicacion de aminoacidos no naturales
EP3249056A3 (en) Isothermal nucleic acid amplification
ES2422583T3 (es) Detección de parvovirus B19
WO2009086608A3 (en) Method and kit to perform a pcr amplification and micro- array detection in the same medium and/or same chamber
BR112016029907A2 (pt) enriquecimento geneticamente adaptado e reação em cadeia da polimerase para a rápida detecção de microrganismos".
EP4296374A3 (en) Detection of infectious agents from environmental air dust
RU2010144789A (ru) Процесс и способ мониторинга желудочно-кишечной микрофлоры
DE602008006254D1 (de) Doppel-oligonukleotid-nukleinsäurenachweisverfahren
SG161271A1 (en) Method for the detection and characterization of microorganisms on a filter
MX387645B (es) Dispositivo y aparato para recoger cultivos microbianos de una superficie semisólida.
ATE525479T1 (de) Spezifische oligonukleotidsequenzen von neisseria gonorrhoeae
WO2007021250A3 (en) Method and/or apparatus of oligonucleotide design and/or nucleic acid detection
MX389572B (es) Amplificacion y deteccion de acidos nucleicos en una muestra biologica.
ES2536734T3 (es) Método para detectar microorganismos del género Kingella
DK1815013T3 (da) Sammensætning til at amplificere nucleinsyrer
EP2757159A3 (en) Microorganism detecting system and microorganism detecting method
EA201491821A1 (ru) Модифицированные олигонуклеотиды, включающие тиольные функциональные группы, и их применение для детекции нуклеиновых кислот
ATE493510T1 (de) Nachweis von neisseria gonorrhoeae
RU2011152586A (ru) ИСПОЛЬЗОВАНИЕ ГЕНОВ СИСТЕМ ТОКСИН-АНТИТОКСИН RelBE И MazEF ДЛЯ ВИДОВОЙ И ШТАММОВОЙ ИДЕНТИФИКАЦИИ ЛАКТОБАЦИЛЛ