ES2528673B1 - Péptido con actividad inhibidora de quorum sensing, polinucleótido que codifica dicho péptido y sus aplicaciones - Google Patents

Péptido con actividad inhibidora de quorum sensing, polinucleótido que codifica dicho péptido y sus aplicaciones Download PDF

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Publication number
ES2528673B1
ES2528673B1 ES201331060A ES201331060A ES2528673B1 ES 2528673 B1 ES2528673 B1 ES 2528673B1 ES 201331060 A ES201331060 A ES 201331060A ES 201331060 A ES201331060 A ES 201331060A ES 2528673 B1 ES2528673 B1 ES 2528673B1
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Prior art keywords
peptide
quorum sensing
activity
applications
polinucleótido
Prior art date
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Expired - Fee Related
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ES201331060A
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English (en)
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ES2528673A1 (es
Inventor
Ana María Otero Casal
Manuel Romero Bernárdez
Celia MAYER MAYER
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Universidade de Santiago de Compostela
Fundacion Pedro Barrie de la Maza Conde de Fenosa
Original Assignee
Universidade de Santiago de Compostela
Fundacion Pedro Barrie de la Maza Conde de Fenosa
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Application filed by Universidade de Santiago de Compostela, Fundacion Pedro Barrie de la Maza Conde de Fenosa filed Critical Universidade de Santiago de Compostela
Priority to ES201331060A priority Critical patent/ES2528673B1/es
Priority to ES14822500T priority patent/ES2742307T3/es
Priority to EP14822500.6A priority patent/EP3020814B1/en
Priority to PCT/ES2014/070569 priority patent/WO2015004305A1/es
Priority to US14/904,278 priority patent/US10316302B2/en
Publication of ES2528673A1 publication Critical patent/ES2528673A1/es
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Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Virology (AREA)
  • Pest Control & Pesticides (AREA)
  • Agronomy & Crop Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Communicable Diseases (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Péptido con actividad inhibidora de Quorum Sensing, polinucleótido que codifica dicho péptido y sus aplicaciones.#Se describe la donación, secuenciación y caracterización del gen responsable de la actividad Quorum Quenching (QQ) frente a señales de Quorum Sensing (QS) de la cepa Tenacibacuium sp. 20J (CECT7426). Dicho gen codifica un péptido que presenta al menos actividad lactonasa con un porcentaje de identidad inferior al 38% con las lactonasas descritas hasta el momento para otras especies, así como las secuencias de los genes homólogos presentes en otras especies del género Tenacibacuium. Dicho péptido muestra un amplio espectro de actividad degradando N-acil-homoserin lactonas (AHLs) de 4-14 átomos de carbono en su cadena lateral, opcionalmente sustituidas, es activo a pH comprendido entre 3 y 9, resistente a proteinasa K y quimiotripsina y no interactúa con antibióticos 13{be}-lactámicos.

Description

β-
ES201331060A 2013-07-11 2013-07-11 Péptido con actividad inhibidora de quorum sensing, polinucleótido que codifica dicho péptido y sus aplicaciones Expired - Fee Related ES2528673B1 (es)

Priority Applications (5)

Application Number Priority Date Filing Date Title
ES201331060A ES2528673B1 (es) 2013-07-11 2013-07-11 Péptido con actividad inhibidora de quorum sensing, polinucleótido que codifica dicho péptido y sus aplicaciones
ES14822500T ES2742307T3 (es) 2013-07-11 2014-07-10 Péptido con actividad inhibidora de quorum sensing, polinucleótido que codifica dicho péptido y los usos de los mismos
EP14822500.6A EP3020814B1 (en) 2013-07-11 2014-07-10 Peptide with quorum-sensing inhibitory activity, polynucleotide that encodes said peptide, and the uses thereof
PCT/ES2014/070569 WO2015004305A1 (es) 2013-07-11 2014-07-10 Péptido con actividad inhibidora de quorum sensing, polinucleótido que codifica dicho péptido y sus aplicaciones
US14/904,278 US10316302B2 (en) 2013-07-11 2014-07-10 Peptide with quorum-sensing inhibitory activity, polynucleotide that encodes said peptide, and the uses thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201331060A ES2528673B1 (es) 2013-07-11 2013-07-11 Péptido con actividad inhibidora de quorum sensing, polinucleótido que codifica dicho péptido y sus aplicaciones

Publications (2)

Publication Number Publication Date
ES2528673A1 ES2528673A1 (es) 2015-02-11
ES2528673B1 true ES2528673B1 (es) 2015-11-24

Family

ID=52279385

Family Applications (2)

Application Number Title Priority Date Filing Date
ES201331060A Expired - Fee Related ES2528673B1 (es) 2013-07-11 2013-07-11 Péptido con actividad inhibidora de quorum sensing, polinucleótido que codifica dicho péptido y sus aplicaciones
ES14822500T Active ES2742307T3 (es) 2013-07-11 2014-07-10 Péptido con actividad inhibidora de quorum sensing, polinucleótido que codifica dicho péptido y los usos de los mismos

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES14822500T Active ES2742307T3 (es) 2013-07-11 2014-07-10 Péptido con actividad inhibidora de quorum sensing, polinucleótido que codifica dicho péptido y los usos de los mismos

Country Status (4)

Country Link
US (1) US10316302B2 (es)
EP (1) EP3020814B1 (es)
ES (2) ES2528673B1 (es)
WO (1) WO2015004305A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10081800B1 (en) * 2017-08-03 2018-09-25 Fornia Biosolutions, Inc. Lactonase enzymes and methods of using same
AU2020295036A1 (en) * 2019-06-17 2022-01-06 Migal Galilee Research Institute Ltd. Stabilized mutants of quorum quenching lactonase and use thereof in treatment of pathogens
CN112481236B (zh) * 2020-11-25 2023-06-06 武汉理工大学 一种重组蛋白INP-AidH及其制备方法和应用
WO2022238627A1 (fr) 2021-05-12 2022-11-17 Museum National D'histoire Naturelle Inhibiteurs de quorum sensing et composition les contenant
IL310353A (en) * 2021-07-22 2024-03-01 Migal Galilee Res Institute Ltd Quorum quenching enzyme, and methods of using same
CN117327600A (zh) * 2023-04-18 2024-01-02 宁波大学 一种对虾肠道海泥黏着杆菌菌株及其在对虾养殖中的应用
CN119241670A (zh) * 2024-09-30 2025-01-03 上海交通大学 一种无细胞催化系统构建优化方法及其应用
CN119056411B (zh) * 2024-11-04 2025-01-14 天科院环境科技发展（天津）有限公司 一种降低活性污泥中抗性基因的活性炭及其制备方法和应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003068951A1 (en) 2002-01-23 2003-08-21 Agency For Science, Technology And Research Ralstonia ahl-acylase gene
ES2342807B2 (es) 2008-08-01 2011-03-18 Universidade De Santiago De Compostela Uso de bacterias del genero tenacibaculum para quorum quenching.

Also Published As

Publication number Publication date
ES2528673A1 (es) 2015-02-11
EP3020814B1 (en) 2019-05-22
EP3020814A4 (en) 2016-11-30
WO2015004305A1 (es) 2015-01-15
US20160244735A1 (en) 2016-08-25
ES2742307T3 (es) 2020-02-13
US10316302B2 (en) 2019-06-11
EP3020814A1 (en) 2016-05-18

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