ES2491965R1 - Procedimiento biológico para la degradación de mezclas complejas de hidrocarburos en fase acuosa - Google Patents

Procedimiento biológico para la degradación de mezclas complejas de hidrocarburos en fase acuosa Download PDF

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Publication number
ES2491965R1
ES2491965R1 ES201330334A ES201330334A ES2491965R1 ES 2491965 R1 ES2491965 R1 ES 2491965R1 ES 201330334 A ES201330334 A ES 201330334A ES 201330334 A ES201330334 A ES 201330334A ES 2491965 R1 ES2491965 R1 ES 2491965R1
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Prior art keywords
hydrocarbons
degradation
biological
degrades
bacterium
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Granted
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ES201330334A
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English (en)
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ES2491965A2 (es
ES2491965B1 (es
Inventor
José Luis NIQUI ARROYO
Sonia FERNÁNDEZ CIFUENTES
Amalia De Arrixaca ROCA HERNÁNDEZ
Jennifer Solano Parada
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Ingenieria y Economia del Transporte SME MP SA
Bio-Iliberis Research and Development SL
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Ingenieria y Economia del Transporte SA
Bio-Iliberis Research and Development SL
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Application filed by Ingenieria y Economia del Transporte SA, Bio-Iliberis Research and Development SL filed Critical Ingenieria y Economia del Transporte SA
Priority to ES201330334A priority Critical patent/ES2491965B1/es
Priority to PCT/ES2014/070170 priority patent/WO2014135735A2/es
Publication of ES2491965A2 publication Critical patent/ES2491965A2/es
Publication of ES2491965R1 publication Critical patent/ES2491965R1/es
Application granted granted Critical
Publication of ES2491965B1 publication Critical patent/ES2491965B1/es
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
    • A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/02—Aerobic processes
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/341—Consortia of bacteria
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/344—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for digestion of mineral oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C12N1/28—Processes using, or culture media containing, hydrocarbons aliphatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • A—HUMAN NECESSITIES
    • A62—LIFE-SAVING; FIRE-FIGHTING
    • A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
    • A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
    • A62D2101/20—Organic substances
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/32—Hydrocarbons, e.g. oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Emergency Management (AREA)
  • Business, Economics & Management (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Molecular Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)

Abstract

Procedimiento biológico para la degradación de mezclas complejas de hidrocarburos en fase acuosa.#El procedimiento biológico para reducir el contenido de hidrocarburos presentes en un medio acuoso que comprende una mezcla de hidrocarburos que contiene, al menos, un alcano alifático; al menos, un hidrocarburo aromático policíclico (HAP); y, al menos, un hidrocarburo aromático seleccionado del grupo formado por benceno, tolueno, etilbenceno, o-xileno, m-xileno, p-xileno y sus combinaciones, comprende poner en contacto dicho medio acuoso contaminado por dicha mezcla de hidrocarburos con una mezcla bacteriana que comprende, al menos, una bacteria que degrada un alcano alifático; al menos una bacteria que degrada un HAP; y, al menos, una bacteria que degrada dicho hidrocarburo aromático, El procedimiento puede incluir un tratamiento químico o fotoquímico previo al biológico del medio acuoso contaminado. Se describen bacterias capaces de degradar dichos hidrocarburos.
ES201330334A 2013-03-08 2013-03-08 Procedimiento biológico para la degradación de mezclas complejas de hidrocarburos en fase acuosa Expired - Fee Related ES2491965B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201330334A ES2491965B1 (es) 2013-03-08 2013-03-08 Procedimiento biológico para la degradación de mezclas complejas de hidrocarburos en fase acuosa
PCT/ES2014/070170 WO2014135735A2 (es) 2013-03-08 2014-03-07 Procedimiento biológico para la degradación de mezclas complejas de hidrocarburos en fase acuosa

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201330334A ES2491965B1 (es) 2013-03-08 2013-03-08 Procedimiento biológico para la degradación de mezclas complejas de hidrocarburos en fase acuosa

Publications (3)

Publication Number Publication Date
ES2491965A2 ES2491965A2 (es) 2014-09-08
ES2491965R1 true ES2491965R1 (es) 2014-12-11
ES2491965B1 ES2491965B1 (es) 2015-11-30

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ES201330334A Expired - Fee Related ES2491965B1 (es) 2013-03-08 2013-03-08 Procedimiento biológico para la degradación de mezclas complejas de hidrocarburos en fase acuosa

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ES (1) ES2491965B1 (es)
WO (1) WO2014135735A2 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105259274A (zh) * 2015-11-18 2016-01-20 云南大学 一种高效液相色谱法测定细菌对菲的降解的方法
EP4107245A4 (en) * 2020-02-19 2024-11-13 The Government of the United States of America, as represented by the Secretary of the Navy BIOFILM BIOREACTOR
CN112725240B (zh) * 2021-02-05 2022-03-29 山东省科学院生态研究所(山东省科学院中日友好生物技术研究中心) 一株活不动杆菌及其菌剂和应用

Citations (3)

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EP2407432A1 (en) * 2010-07-14 2012-01-18 Söll GmbH Composition for triggering microbiological processes in water and method of producing the same
US20120178146A1 (en) * 2009-04-03 2012-07-12 IFP Energies Nouvelles Bacteria capable of degrading multiple petroleum compounds in solution in aqueous effluents and process for treating said effluents
WO2012137220A2 (en) * 2011-04-04 2012-10-11 Indian Oil Corporation Ltd. Bio-augmentation composition and use thereof for improving efficiency of effluent treatment in hydrocarbon processing plant

Family Cites Families (3)

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Publication number Priority date Publication date Assignee Title
JP5251003B2 (ja) * 2007-05-28 2013-07-31 東ソー株式会社 潤滑油分解微生物および微生物コンソーシアム、ならびにそれらを用いた潤滑油汚染土壌の浄化方法
CN102250794B (zh) * 2011-06-21 2013-11-06 中国农业科学院农业环境与可持续发展研究所 一种日本假黄单胞菌和微生物菌剂及它们的应用
CN102250801B (zh) * 2011-06-22 2013-11-06 中国农业科学院农业环境与可持续发展研究所 一种微生物菌剂及其制备方法和应用

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
US20120178146A1 (en) * 2009-04-03 2012-07-12 IFP Energies Nouvelles Bacteria capable of degrading multiple petroleum compounds in solution in aqueous effluents and process for treating said effluents
EP2407432A1 (en) * 2010-07-14 2012-01-18 Söll GmbH Composition for triggering microbiological processes in water and method of producing the same
WO2012137220A2 (en) * 2011-04-04 2012-10-11 Indian Oil Corporation Ltd. Bio-augmentation composition and use thereof for improving efficiency of effluent treatment in hydrocarbon processing plant

Non-Patent Citations (5)

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Also Published As

Publication number Publication date
WO2014135735A2 (es) 2014-09-12
ES2491965A2 (es) 2014-09-08
WO2014135735A3 (es) 2014-11-06
ES2491965B1 (es) 2015-11-30

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