ES2482666A1 - Uso de la cepa CECT 7426 para provocar quorum quenching de la señal autoinductor-2 (AI-2) - Google Patents

Uso de la cepa CECT 7426 para provocar quorum quenching de la señal autoinductor-2 (AI-2) Download PDF

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ES2482666A1
ES2482666A1 ES201231552A ES201231552A ES2482666A1 ES 2482666 A1 ES2482666 A1 ES 2482666A1 ES 201231552 A ES201231552 A ES 201231552A ES 201231552 A ES201231552 A ES 201231552A ES 2482666 A1 ES2482666 A1 ES 2482666A1
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Ana María Otero Casal
Manuel Romero Bernárdez
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Universidade de Santiago de Compostela
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    • A—HUMAN NECESSITIES
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    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
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Abstract

Uso de la cepa CECT 7426 para provocar quorum quenching de la señal Autoinductor-2 (AI-2). La invención se refiere al uso de una cepa bacteriana de la especie Tenacibaculum discolor, del extracto celular crudo o del sobrenadante de sus cultivos, para provocar quorum quenching en bacterias a través de la inhibición de señales tipo AI-2, y más concretamente para el tratamiento y/o prevención de enfermedades infecciosas y para inhibir la formación de biofilms, producidos por dichas bacterias.

Description

imagen1
imagen2
La presente invención describe por primera vez la capacidad de una cepa bacteriana de Tenacibaculum discolor para interferir con las señales de quorum sensing de tipo AI-2, y por tanto para inhibir procesos de virulencia o de formación de biofilms por parte de importantes patógenos.
5 Por tanto, un aspecto de la invención se refiere al uso de una cepa bacteriana depositada en la Colección Española de Cultivos Tipo (CECT) con número de depósito CECT 7426, del extracto celular crudo o del sobrenadante de sus cultivos, o cualquiera de sus combinaciones, de ahora en adelante “cepa de la invención” o “cepa 20J”, para provocar quorum quenching en bacterias productoras de la señal AI-2.
10 La cepa depositada en la Colección Española de Cultivos Tipo (CECT) con número de depósito CECT 7426 es la cepa descrita en la solicitud de patente ES2342807B2.
Cualquier bacteria regula su expresión génica en respuesta a diferentes señales medioambientales, una propiedad esencial para competir con otros organismos. En el caso particular de bacterias patógenas, la 15 regulación génica es crucial para permitir la supervivencia de la bacteria en el particular ambiente que le ofrece su hospedador. Los genes de virulencia bacterianos están sujetos a complejos mecanismos de regulación para asegurar la expresión del gen apropiado en el momento apropiado. Los AI-2 son las señales de QS más extendidas en bacterias de todo tipo, y son empleadas por multitud de bacterias patógenas humanas, de plantas y marinas, tanto Gram-positivas como Gram-negativas, para el control de
20 la producción de factores de virulencia.
La proteína sintetizadora de AI-2 es LuxS. Una búsqueda en la base de datos GenBank demuestra que existen numerosas bacterias que poseen homólogos del gen luxS de Vibrio harveyi, la especie de referencia en el estudio de este tipo de señales, y que por tanto son bacterias productoras de AI-2. Las
25 bacterias que poseen homólogos del gen luxS de V. harveyi se muestran en la Tabla 1.
PROTEOBACTERIA
ESPECIE
Porcentaje de homología (%) con el gen luxS de V. harveyi
Vibrio harveyi ATCC BAA-1116
100
Aliivibrio logei
98
Aliivibrio salmonicida LFI1238
86
Alishewanella agri BL06
80
Citrobacter koseri ATCC BAA-895
78
Citrobacter rodentium ICC168
78
Citrobacter sp. 30_2
78
Cronobacter sakazakii ES1
77
Cronobacter turicensis z3032
78
Dickeya dadantii 3937
78
Enterobacter cancerogenus ATCC 35316
77
Enterobacter cloacae EcWSU1
77
Enterobacter hormaechei ATCC 49162
77
Enterobacter mori LMG 25706
77
Enterobacter radicincitans DSM 16656
77
Enterobacter sp. Ag1
78
Escherichia albertii TW07627
79
Escherichia coli CFT073
78
Escherichia coli O157:H7 str. EDL933
77
Escherichia hermannii NBRC 105704
77
Ferrimonas balearica DSM 9799
82
Haemophilus parainfluenzae ATCC 33392
78
Haemophilus pittmaniae HK 85
78
Moritella sp. PE36
78
Neisseria cinerea ATCC 14685
81
Neisseria flavescens NRL30031/H210
80
Neisseria gonorrhoeae FA 1090
81
Neisseria lactamica ATCC 23970
81
Neisseria macacae ATCC 33926
80
Neisseria meningitidis NM3081
81
Neisseria mucosa ATCC 25996
80
Neisseria polysaccharea ATCC 43768
81
Neisseria sicca ATCC 29256
80
Neisseria subflava NJ9703
80
Oceanimonas sp. GK1
79
Pantoea sp. aB
76
Photobacterium leiognathi subsp. mandapamensis svers.1.1
81
Photobacterium phosphoreum
78
Photobacterium profundum 3TCK
85
Proteus mirabilis HI4320
78
Proteus penneri ATCC 35198
78
Providencia stuartii MRSN 2154
78
Rheinheimera nanhaiensis E407-8
81
Salmonella bongori NCTC 12419
77
Salmonella enterica subsp. arizonae serovar 62:z4,z23 str. RSK2980
76
Serratia odorifera DSM 4582
77
Serratia proteamaculans 568
76
Shewanella amazonensis SB2B
78
Shewanella baltica OS625
78
Shewanella benthica KT99
78
Shewanella halifaxensis HAW-EB4]
76
Shewanella loihica PV-4
78
Shewanella oneidensis MR-1
78
Shewanella pealeana ATCC 700345
77
Shewanella piezotolerans WP3
77
Shewanella sediminis HAW-EB3
77
Shewanella violacea DSS12
78
Shewanella woodyi ATCC 51908
78
Shigella flexneri 1235-66
78
Vibrio aestuarianus
85
Vibrio alginolyticus 12G01
95
Vibrio anguillarum 775
85
Vibrio angustum S14
82
Vibrio brasiliensis LMG 20546
91
Vibrio campbellii DS40M4
99
Vibrio cholerae O1 biovar El Tor str. N16961
86
Vibrio cincinnatiensis
86
Vibrio diazotrophicus
87
Vibrio fischeri ES114
87
Vibrio fluvialis
85
Vibrio furnissii
85
Vibrio ichthyoenteri ATCC 700023
87
Vibrio mediterranei
89
Vibrio metschnikovii
84
Vibrio mimicus VM603
85
Vibrio nereis
89
Vibrio nigripulchritudo ATCC 27043
85
Vibrio ordalii ATCC 33509
85
Vibrio parahaemolyticus RIMD 2210633
94
Vibrio pomeroyi
90
Vibrio scophthalmi LMG 19158
88
Vibrio shilonii AK1
88
Vibrio sinaloensis DSM 21326
89
Vibrio splendidus LGP32
90
Vibrio tubiashii
90
Vibrio vulnificus CMCP6
90
Vibrio xuii
92
Yersinia rohdei ATCC 43380
79
SPIROCHAETALES, FIRMICUTES, ACTINOBACTERIDAE
ESPECIE
Porcentaje de homología (%) con el gen luxS de V. harveyi
Vibrio harveyi ATCC BAA-1116
100
Acetohalobium arabaticum DSM 5501
83
Actinomyces georgiae F0490
94
Actinomyces odontolyticus ATCC 17982
93
Actinomyces sp. oral taxon 178 str. F0338
94
Aerococcus viridans ATCC 11563
93
Alkaliphilus oremlandii OhILAs
84
Arthrobacter arilaitensis Re117
91
Bacillus cellulosilyticus DSM 2522
83
Bacillus coagulans 36D1
83
Bacillus macauensis ZFHKF-1
83
Bacillus selenitireducens MLS10
83
Bacillus smithii 7_3_47FAA
83
Bifidobacterium longum subsp. longum BBMN6
41
Bifidobacterium breve CECT 7263
41
Brachybacterium faecium DSM 4810
84
Clostridium botulinum A3 str. Loch Maree
88
Clostridium cellulovorans 743B
88
Clostridium perfringens ATCC 13124
88
Clostridium sporogenes ATCC 15579
88
Enterococcus faecium E980
89
Gemella haemolysans ATCC 10379
84
Gemella morbillorum M424
84
Gemella sanguinis M325
84
Kurthia sp. JC8E
91
Lactobacillus amylovorus GRL 1112
88
Lactobacillus buchneri NRRL B-30929
88
Lactobacillus crispatus JV-V01
88
Lactobacillus farciminis KCTC 3681
88
Lactobacillus gasseri MV-22
88
Lactobacillus helveticus H10
88
Lactobacillus johnsonii NCC 533
88
Lactobacillus kefiranofaciens ZW3
88
Lactobacillus mucosae LM1
88
Lactobacillus ultunensis DSM 16047
88
Lactobacillus versmoldensis KCTC 3814
88
Lentibacillus sp. Grbi
83
Listeria monocytogenes HCC23
83
Oenococcus oeni PSU-1
88
Ornithinibacillus scapharcae TW25
83
Paenibacillus alvei DSM 29
88
Pediococcus pentosaceus ATCC 25745
88
Planococcus antarcticus DSM 14505
84
Planococcus donghaensis MPA1U2
83
Propionibacterium acnes KPA171202
83
Ruminococcus flavefaciens
46
Solibacillus silvestris StLB046
83
Sporosarcina newyorkensis 2681
83
Staphylococcus arlettae CVD059
83
Staphylococcus aureus RF122
84
Staphylococcus capitis SK14
84
Staphylococcus caprae C87
84
Staphylococcus carnosus subsp. carnosus TM300
84
Staphylococcus epidermidis VCU121
84
Staphylococcus haemolyticus JCSC1435
84
Staphylococcus hominis VCU122
84
Staphylococcus lugdunensis HKU09-01
83
Staphylococcus pettenkoferi VCU012
84
Staphylococcus pseudintermedius ED99
84
Staphylococcus saprophyticus subsp. saprophyticus ATCC 15305
84
Staphylococcus simiae CCM 7213
88
Staphylococcus warneri L37603
84
Streptococcus anginosus SK52 = DSM 20563
40
Streptococcus criceti HS-6
40
Streptococcus cristatus ATCC 51100
38
Streptococcus gordonii str. Challis substr. CH1
38
Streptococcus mutans NN2025
38
Streptococcus parasanguinis F0405
37
Streptococcus ratti FA-1
37
Streptococcus sanguinis SK405
39
Thermoanaerobacterium saccharolyticum JW/SL-YS485
84
Thermoanaerobacterium thermosaccharolyticum DSM 571
84
Campylobacter jejuni subsp. jejuni CG8486
75
Campylobacter coli 317/04
75
Corynebacterium casei UCMA 3821
37
Corynebacterium pseudogenitalium ATCC 33035
39
Corynebacterium ammoniagenes DSM 20306
38
Corynebacterium tuberculostearicum SK141
39
Corynebacterium aurimucosum ATCC 700975
38
Corynebacterium glucuronolyticum ATCC 51867
38
Corynebacterium accolens ATCC 49725
38
Corynebacterium kroppenstedtii DSM 44385
39
Corynebacterium striatum ATCC 6940
35
Corynebacterium glutamicum ATCC 13032
29
Gordonia polyisoprenivorans NBRC 16320
41
Mycobacterium sp. MOTT36Y
33
Mycobacterium intracellulare ATCC 13950
33
Helicobacter cinaedi CCUG 18818
68
Helicobacter hepaticus ATCC 51449
69
Helicobacter bilis ATCC 43879
66
Helicobacter winghamensis ATCC BAA-430
70
Helicobacter canadensis MIT 98-5491
66
Helicobacter pullorum MIT 98-5489
65
Helicobacter pylori B38
41
Borrelia valaisiana VS116
33
Borrelia burgdorferi B31
33
Borrelia bissettii DN127
33
Borrelia afzelii PKo
33
Borrelia garinii BgVir
33
Actinobacillus minor NM305
74
Actinobacillus pleuropneumoniae serovar 1 str. 4074]
72
Actinobacillus ureae ATCC 25976
72
Actinobacillus succinogenes 130Z
72
Prevotella ruminicola
100
Prevotella brevis
96
Prevotella tannerae ATCC 51259
34
Prevotella bryantii B14
32
Porphyromonas asaccharolytica PR426713P-I
34
Prevotella buccae ATCC 33574
32
Porphyromonas uenonis 60-3
34
Prevotella ruminicola 23
33
Prevotella bergensis DSM 17361
31
Prevotella multisaccharivorax DSM 17128
32
Prevotella melaninogenica ATCC 25845
33
Prevotella marshii DSM 16973
33
Prevotella oralis ATCC 33269
31
Prevotella timonensis CRIS 5C-B1
32
Prevotella dentalis DSM 3688
33
Porphyromonas gingivalis ATCC 33277
29
Tabla 1: Bacterias que poseen homólogos del gen luxS (gen responsable de la producción de la señal AI2) de V. harveyi (GenBank).
Además, existen bacterias no mostradas en la Tabla 1 pero para las que ya se ha demostrado un efecto
5 fisiológico de AI-2 y/o de la mutación de su sintetasa (LuxS), tales como Serratia marcescens, Streptococcus pneumoniae, Streptococcus oralis, Streptococcus pyogenes, Streptococcus suis, Streptococcus intermedius, Eikenella corrodens, Bacillus subtilis, Klebsiella pneumoniae, Photorhabdus luminescens, Bacteroides sp., Aeromonas hydrophila, Haemophilus influenzae, Lactobacillus rhamnosus y Lactobacillus reuteri (Vendeviile, A. et al. 2005. Nature Reviews Microbiology 3:383-396; Hardie, K.R. and
10 Heurlier, K. 2008. Nature Reviews Microbiology 6:635-643).
Por tanto, en la presente invención se entiende por “bacteria productora de AI-2” cualquier especie seleccionada de la lista que consiste en: Actinomyces georgiae, Actinomyces odontolyticus, Actinomyces sp., Aerococcus viridans, Alkaliphilus oremlandii, Arthrobacter arilaitensis, Bacillus cellulosilyticus, Bacillus 15 coagulans, Bacillus macauensis, Bacillus selenitireducens, Bacillus smithii, Bacillus subtilis, Bifidobacterium longum, Bifidobacterium breve, Brachybacterium faecium, Corynebacterium accolens, Corynebacterium ammoniagenes, Corynebacterium aurimucosum, Corynebacterium casei, Corynebacterium glucuronolyticum, Corynebacterium glutamicum, Corynebacterium kroppenstedtii, Corynebacterium pseudogenitalium, Corynebacterium striatum, Corynebacterium tuberculostearicum, 20 Clostridium botulinum, Clostridium cellulovorans, Clostridium perfringens, Clostridium sporogenes, Enterococcus faecium, Gemella haemolysans, Gemella morbillorum, Gemella sanguinis, Gordonia polyisoprenivorans, Kurthia sp., Lactobacillus amylovorus, Lactobacillus buchneri, Lactobacillus crispatus, Lactobacillus farciminis, Lactobacillus gasseri, Lactobacillus helveticus, Lactobacillus johnsonii, Lactobacillus kefiranofaciens, Lactobacillus mucosae, Lactobacillus rhamnosus, Lactobacillus reuteri, 25 Lactobacillus ultunensis, Lactobacillus versmoldensis, Lentibacillus sp., Listeria monocytogenes, Mycobacterium sp., Mycobacterium intracellulare, Oenococcus oeni, Ornithinibacillus scapharcae, Paenibacillus alvei, Pediococcus pentosaceus, Planococcus antarcticus, Planococcus donghaensis, Propionibacterium acnes, Ruminococcus flavefaciens, Solibacillus silvestris, Sporosarcina newyorkensis, Staphylococcus arlettae, Staphylococcus aureus, Staphylococcus capitis, Staphylococcus caprae, 30 Staphylococcus carnosus, Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus lugdunensis, Staphylococcus pettenkoferi, Staphylococcus pseudintermedius, Staphylococcus saprophyticus, Staphylococcus simiae, Staphylococcus warneri, Streptococcus
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ES201231552A 2012-10-09 2012-10-09 Uso de la cepa CECT 7426 para provocar quorum quenching de la señal autoinductor-2 (AI-2) Active ES2482666B1 (es)

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PCT/ES2013/070662 WO2014057151A1 (es) 2012-10-09 2013-09-23 Uso de la cepa cect 7426 para provocar quorum quenching de la señal autoinductor-2 (ai-2)

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Cited By (3)

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CN110012941A (zh) * 2019-05-23 2019-07-16 光明乳业股份有限公司 一种由类芽孢杆菌制备发酵豆浆的方法及其制备出的发酵豆浆与应用
CN111419829A (zh) * 2020-03-02 2020-07-17 东北农业大学 和厚朴酚在抑制猪链球菌或其生物被膜中的用途
CN113368137A (zh) * 2021-05-06 2021-09-10 华南农业大学 一种天然的细菌群体感应抑制剂及其应用

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CN108452667B (zh) * 2018-05-14 2019-03-19 大江环境股份有限公司 一种包含涅氏短状杆菌的生物活性填料
CN108753646B (zh) * 2018-06-12 2021-10-22 上海交通大学医学院附属仁济医院 一株对革兰阳性耐药菌具有广谱抗菌活性的人葡萄球菌及其筛选方法和应用

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110012941A (zh) * 2019-05-23 2019-07-16 光明乳业股份有限公司 一种由类芽孢杆菌制备发酵豆浆的方法及其制备出的发酵豆浆与应用
CN110012941B (zh) * 2019-05-23 2022-07-15 光明乳业股份有限公司 一种由类芽孢杆菌制备发酵豆浆的方法及其制备出的发酵豆浆与应用
CN111419829A (zh) * 2020-03-02 2020-07-17 东北农业大学 和厚朴酚在抑制猪链球菌或其生物被膜中的用途
CN111419829B (zh) * 2020-03-02 2023-08-18 东北农业大学 和厚朴酚在抑制猪链球菌或其生物被膜中的用途
CN113368137A (zh) * 2021-05-06 2021-09-10 华南农业大学 一种天然的细菌群体感应抑制剂及其应用
CN113368137B (zh) * 2021-05-06 2023-08-18 华南农业大学 一种天然的细菌群体感应抑制剂及其应用

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